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Citations for GE Life Sciences :
1851 - 1900 of 2507 citations for 2 4 Methyl 5 thiazolyl ethyl decanoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... The unbound fluorophores were removed by gel filtration on a NAP-5 column (Sephadex-25 medium, GE Healthcare, Munich, Germany). The final antibody concentration and degree of labelling was analyzed on a spectrophotometer (NanoDrop 2000 UV-Vis ...
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bioRxiv - Biochemistry 2022Quote: ... monomeric C9 samples were passed through a Superdex 200 Increase 5/150 GL size-exclusion column (GE Healthcare Life Sciences) in 10 mM HEPESNaOH ...
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bioRxiv - Cell Biology 2022Quote: ... The resultant supernatant was filtered using a 0.45 µm filter and the protein was subsequently purified by IMAC on a 5 ml HP HisTrap column (GE Healthcare) according to manufacturer’s information ...
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bioRxiv - Immunology 2024Quote: ... The lysate was clarified by centrifugation at 47,000 x g for 45min and applied to 5 ml His-Trap columns (GE Healthcare). After washing with a buffer excluding urea ...
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bioRxiv - Molecular Biology 2023Quote: ... Then the samples were subjected to electrophoresis through 5% native polyacrylamide gel in 0.5x TBE buffer and imaged with 9410 Typhoon variable mode imager (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... pH 8.0) and further purified via size exclusion chromatography (SEC) in an S75 5/150 GL increase column (GE Healthcare). Protein collected from SEC was normalized to 1 μM where possible.
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bioRxiv - Biophysics 2024Quote: ... HRP-secondary antibodies were incubated for 1-2 hours at room temperature in 5% milk and ECL were performed using the Amersham ECL Western Blotting Detection Reagents (Cat.no. RPN2106 from GE Healthcare). Detection was done using the Chemidoc XRS imaging system (Bio-Rad)..
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bioRxiv - Microbiology 2023Quote: ... the KhpA and KhpB-ΔN were secondly purified by cation-exchange chromatography (HiTrap SP 5 ml, GE Healthcare Life Science) followed by SDS-PAGE ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was filtered in 0.45 µm filter and loaded into a 5 ml His-trap FF column (GE Healthcare) pre-equilibrated with buffer A (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were incubated with secondary antibodies conjugated with horseradish peroxidase (HRP) diluted in 5% skim milk in TBS-T (1:10,000, GE healthcare). Labelled proteins were detected via enhanced chemiluminescence (Pierce ECL Western Blotting Substrate ...
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bioRxiv - Molecular Biology 2022Quote: ... Cleared lysates were incubated with 5 ml bed volume Glutathione Sepharose 4B resin (GE Healthcare, cat. No. 17-0756-05) for 1 hour ...
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bioRxiv - Biochemistry 2022Quote: ... the clarified material was filtered with a 0.22 µm membrane and then applied a HisTrap HP 5 ml (GE Healthcare) and eluted with buffer B (30 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... 3 mM b-mercaptoethanol (BME) and then loaded onto a HiTrap SP HP 5 ml column (GE healthcare, Piscataway, NJ). Elution from the SP column was performed with a 70 ml linear gradient from 0–500 mM NaCl in SP buffer A ...
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bioRxiv - Microbiology 2022Quote: ... We pelleted and lysed bacteria by sonication in lysis buffer (Tris 100mM, NaCl 0.5M, glycerol 5%) and eGFP was purified using fast flow His-affinity columns (GE Healthcare) and eluted with 0.5 M imidazole ...
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bioRxiv - Cell Biology 2022Quote: ... was then loaded to a 5 mL HisTrap Ni-NTA column coupled to AKTA Pure protein purification system (GE Healthcare). The His-SUMO-tagged CAP1 protein was eluted from the nickel column with an imidazole gradient using buffer A and buffer B (buffer A + 250 mM imidazole) ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was filtered through a 0.45 μm syringe filter and loaded onto a 5 mL HisTrap HP column (GE Healthcare) equilibrated in binding buffer ...
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bioRxiv - Biophysics 2022Quote: ... pH 7.5) for subsequent anion exchange chromatography on a 5 ml Mono-Q column (GE Healthcare Europe GmbH, Freiburg, Germany). Following an elution gradient with high-salt buffer (20mM TRIS ...
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bioRxiv - Cell Biology 2022Quote: ... Data were collected following fractionation with an in-line size-exclusion chromatography column (Superdex 200 5/150 Increase, GE Healthcare,) pre-equilibrated in TBS pH 8.5 ...
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bioRxiv - Immunology 2022Quote: ... Nb-Fc was diluted to a concentration of 5 μg/ml with sodium acetate (pH 4.5) and immobilized on a CM5 chip (GE Healthcare) at a level of ∼150 response units ...
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bioRxiv - Biochemistry 2022Quote: ... Histones were then further purified by cation exchange on a 5 ml HiTrap SP HP column (GE Healthcare Life Sciences), pre-equilibrated with HGFB150 (full-length histones and tailless H2B) ...
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bioRxiv - Microbiology 2022Quote: ... 0.5 mM dithiothreitol (DTT), and concentrated using Vivaspin 5 kDa (HipBLp, HipSLp) and 10 kDa (HipTLp) cutoff columns (GE Healthcare). Protein concentration was quantified using the Bradford assay according to the manufacturer’s instructions (BioShop) ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was passed through a 0.22 µm filter and loaded onto a 5 ml HisTrap HP column (GE Healthcare). The column was washed with 25 ml of lysis buffer containing 30 mM imidazole ...
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bioRxiv - Biochemistry 2023Quote: Elute from HisTrap consisting of the respective protein fractions were centrifuged at 39,000 g for 15 minutes and the supernatant was loaded on a 5 ml StrepTrap HP column (GE Healthcare). The binding buffer used was 150 mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... the supernatant was diluted 5-fold to contain 100 mM NaCl and purified over SP-Sepharose (HiPrep SPFF, GE Healthcare) using a linear gradient of buffer containing 2 M NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... the supernatant was loaded in an ÄKTA Pure purification system (Cytiva) onto a 5 ml HiTrap Chelating HP (GE Healthcare) charged with 0.1 mM NiCl2 and equilibrated with wash buffer (50 mM K-HEPES pH7.5 ...
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bioRxiv - Biophysics 2023Quote: ... Double-labeled (488:594) protein was then further purified via ion-exchange chromatography (Mono S 5/50 GL, GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... Eluted fractions containing the desired protein were pooled and loaded onto a 5 ml cationic exchanger (S-column; GE Healthcare). A salt gradient of 0-1 M was applied over 20 min and the protein was eluted in 5 ml fractions ...
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bioRxiv - Biophysics 2023Quote: ... HRP-secondary antibodies were incubated for 1-2 hours at room temperature in 5% milk and ECL were performed using the Amersham ECL Western Blotting Detection Reagents (Cat.no. RPN2106 from GE Healthcare). Detection was done using CL-Xposure films (Cat.no ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted fractions containing the desired protein were pooled and loaded onto a 5 ml cationic exchanger (S-column; GE Healthcare). A salt gradient of 0-1 M KCl was applied over 20 min (at 5 ml/min ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was filtered through a MF-Millipore Membrane Filter (0.45 µm pore size) then loaded on a 5 mL His-Trap HP column (GE Healthcare) pre-equilibrated with buffer A and CSE was eluted with a gradient from 20 mM to 250 mM imidazole in buffer A ...
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bioRxiv - Cell Biology 2022Quote: ... the supernatant was loaded in an ÄKTA Pure purification system (Cytiva) onto a 5 ml HiTrap Chelating HP (GE Healthcare) charged with 0.1 mM NiCl2 ...
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bioRxiv - Molecular Biology 2024Quote: ... MukBEF was largely found in the flowthrough and was applied to a 5 mL HiTrap Q HP column (GE Healthcare). The column was washed with 2 CV of 10 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... the supernatant was collected and applied to a 5 mL pre-equilibrated HiTrap Phenyl HP hydrophobic interaction column (GE Healthcare) using a running buffer with 20 mM Tris-HCl ...
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bioRxiv - Immunology 2024Quote: ... 5 mL of the samples were applied to a size exclusion chromatography column (Superdex 200pg, HiLoad 16/60, GE healthcare) equilibrated in SEC buffer (20 mM HEPES ...
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bioRxiv - Biophysics 2024Quote: ... the protein was filtered and loaded onto two connected 5 ml HiTrap Q HP columns (GE Healthcare, 17- 1154-01) equilibrated with 10 mM Tris-HCl ...
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bioRxiv - Biophysics 2024Quote: ... Double-labeled (488:594) protein was then further purified via ion-exchange chromatography (Mono Q 5/50 GL, GE Healthcare).
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bioRxiv - Bioengineering 2024Quote: ... the soluble fraction of the cell lysate was passed through a 0.45-micron filter and then loaded onto a HisTrap HP 5 ml (GE Healthcare) column equilibrated with buffer A (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: ... The solubilized protein suspension was ultracentrifuged at 100,000 x g for 1 hour before being loaded onto a 5 ml HisTrap HP column (GE HealthCare). The column was washed with a purification buffer supplemented with 20 mM imidazole ...
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bioRxiv - Biochemistry 2024Quote: The Ni-NTA elution was immediately loaded onto a 5 mL HiTrap Heparin HP column (GE Healthcare, Chicago, IL, USA) that was equilibrated with 20 mM HEPES (pH 7.5) ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was filtered through an 0.8 µm membrane and loaded onto a 5 mL HisTrap HP column (GE Healthcare), pre-equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2024Quote: ... and the sample was loaded on a 5 ml HiTrap™ Phenyl Sepharose High-Performance column (GE Healthcare, Munich, Germany). The sample was eluted at 1 M (NH4)2SO4 at a flow rate of 1 ml.min-1 ...
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bioRxiv - Biophysics 2024Quote: ... RbmB was purified from the cleared lysate by loading the supernatant onto a 5 mL His-trap HF (GE Healthcare) equilibrated with high-salt TBS buffer (20 mM Tris pH 7.6 ...
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bioRxiv - Bioengineering 2024Quote: ... The eluate was then exchanged into 20 mM Tris HCl pH 8.0 by dialysis and loaded onto a 5 mL Q-HP column (GE Healthcare). The different forms were isolated by using 50 column volumes (250 mL ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was further purified by salt gradient ion exchange chromatography using a Mono Q 5/50 GL column (GE healthcare) and Gradient buffers (20 mM HEPES-KOH pH 7.6 ...
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bioRxiv - Biochemistry 2024Quote: ... The resultant supernatant was filtered through a 0.8 μm membrane before being loaded onto 5 mL HisTrap FF nickel column (GE Healthcare) pre-equilibrated in lysis buffer ...
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bioRxiv - Biochemistry 2024Quote: ... The soluble material was subsequently filtered using a 0.22 mm pore size filter and was loaded onto a 5 ml HisTrap HP column (GE Healthcare), which was pre-equilibrated with lysis buffer and immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were centrifuged and filtered prior to successive FPLC purification on HisTrap HP (5 mL, GE Healthcare, 17-1154-01), HiTrap Q HP (5 mL ...
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bioRxiv - Biophysics 2021Quote: ... Polyacrylamide gels were polymerized between two glass coverslips treated with 2% dimethyldichlorosilane (Plus One Repel Silane, GE Healthcare). For 0.6 kPa gels ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM DTT at a flow rate of 2 ml/min using an Akta purifier system (GE Healthcare), eluting at approximately 100 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... were subjected to the short column with 2 ml Sephacryl S-500 resin (GE Healthcare Life Sciences, IL) equilibrated with PBS ...