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Citations for GE Life Sciences :
151 - 200 of 231 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... were resuspended in 500 μl IP buffer and incubated with the appropriate antibodies on ice for 2 h and then 30 μl protein-G-sepharose beads (GE Healthcare; equilibrated once in 10 ml water and three times in washing buffer ...
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bioRxiv - Microbiology 2022Quote: ... Radioactive signal was visualized by exposing 4 h a Storage Phosphor Screen BAS-IP MS 2040 (Fujifilm) then imaged with a Typhoon FLA 9500 (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... was incubated 2 h at a dilution of 1:10,000 and revealed by ECL Prime colorimetric assay (GE Healthcare, Chicago, USA).
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bioRxiv - Biochemistry 2022Quote: ... secondary antibodies were incubated for 2 h at room temperature and visualized on X-ray films using enhanced chemiluminescence detection kit (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... The mixture was incubated for 3 h at room temperature and loaded onto a Superdex 200 10/300 GL (GE Healthcare) sizeexclusion chromatography column using buffer B as mobile phase ...
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bioRxiv - Cell Biology 2022Quote: ... were acquired every 1–3 min over a total time period of 4–6 h and deconvolved using the softWoRx software (Applied Precision). In addition ...
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bioRxiv - Microbiology 2022Quote: ... The remaining lysate supernatant was incubated for 2 h on a spinning wheel with 200 µl of high-Performance strepTactin Sepharose beads (#28-9355-99, GE healthcare). The beads were collected by centrifugation at 1600 g at 4°C for 5 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Two hundred and fifty micrograms of chromatin DNA were subjected to a 1 h preclearing with 50 μl of a 50% protein G-Sepharose (GE healthcare) bead slurry containing 1% BSA ...
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bioRxiv - Plant Biology 2022Quote: ... coli strain BL21 with protein induction when OD600 reached 0.5 with 1 mM IPTG (isopropyl b-D-1-Thiogalactopyranoside) for 4 h at 37°C and then purified over a glutathione Sepharose 4B column (GE Healthcare). The eluted samples were then resolved by SDS-PAGE and stained by Coomassie brilliant blue R-250 to evaluate purity ...
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bioRxiv - Molecular Biology 2024Quote: ... The cleared lysate was rotated for 1 h at 4 °C in presence of 50 µL of streptavidin sepharose (50% slurry, GE Healthcare). Beads were washed three times with NET buffer ...
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bioRxiv - Biophysics 2024Quote: ... Samples were run for 2 h at 90 V and then scanned using a Typhoon FLA 9500 laser scanner (GE Healthcare) at a pixel size of 50 µm ...
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bioRxiv - Microbiology 2023Quote: ... membranes were incubated for 1 h at room temperature with secondary antibody (anti-mouse IgG, 1:10,000 in 3% BSA/TBS-T; GE Healthcare, #RPN4201) linked to horseradish peroxidase ...
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bioRxiv - Biochemistry 2023Quote: ... and 2 mM DTT) for 16 h before further purification by anion exchange chromatography on a HiTrap Q HP column (GE Healthcare), followed by size exclusion chromatography (SEC ...
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bioRxiv - Neuroscience 2023Quote: ... lysates were incubated with the primary antibody at 4°C for 2 h or overnight and then incubated with protein G- or protein A-Sepharose (GE Healthcare) for 2 h ...
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bioRxiv - Molecular Biology 2023Quote: ... Two hundred and fifty micrograms of chromatin DNA were subjected to a 1 h preclearing with 50 μl of a 50% protein G-Sepharose (GE healthcare) bead slurry containing 1% BSA ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were clarified by centrifugation at 50,000 × g for 1 h at 4 °C and the supernatant was used for affinity purification on HisTrap FF columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were then incubated for 2-h at room temperature with rabbit HRP-linked IgG (GE Healthcare, Chicago, IL, USA), and a chemiluminescence detection reagent (SuperSignal™ West Pico PLUS Chemiluminescent Substrate ...
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bioRxiv - Molecular Biology 2023Quote: ... Images were acquired with 200 ms exposure time every 6 minutes for at least 6 h at 30°C using Softworx (Applied Precision) software ...
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bioRxiv - Cell Biology 2022Quote: ... membranes were washed three times with TBS-Tween 1 % and incubated for 1 h at room temperature with a secondary antibody anti-Mouse conjugated to horseradish peroxidase (GE Healthcare, NA9310 ...
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bioRxiv - Microbiology 2023Quote: ... The gel was dried at 80°C for 1 h prior to exposure to a phosphor-screen for ∼5 h to overnight and imaged using a Typhoon Trio Variable Mode Imager (GE Healthcare).
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg lysates were incubated with the appropriate agarose-conjugated primary antibody for 3-4 h at 4°C or with unconjugated antibody (1-2 mg) overnight at 4°C followed by 1 h incubation with Protein G Sepharose beads (GE Healthcare). Immuno-complexes were washed four times with NETN buffer (20 mM Tris ...
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bioRxiv - Biophysics 2023Quote: ... Eluted protein was digested with Ulp1 protease at 4°C for 2 h and then further purified on a Heparin HP column (GE Healthcare), eluting with a linear gradient of increasing NaCl concentration from 300 mM to 1 M in 20 mM Tris-HCl ...
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bioRxiv - Biophysics 2023Quote: ... Eluted protein was digested with Ulp1 protease at 4°C for 2 h and then further purified on a Heparin HP column (GE Healthcare), eluting with buffer containing 20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2024Quote: ... Parasites were synchronized to a 3-h age window by isolating late schizonts from a 60% Percoll (GE Healthcare, Life Sciences) gradient and culturing these for 3 h with fresh erythrocytes (104) ...
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bioRxiv - Microbiology 2024Quote: ... Plates were incubated at 30 °C for 48 h and pictures were taken with Amersham Imager 600 (GE Healthcare Life Sciences).
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bioRxiv - Microbiology 2024Quote: ... The 6ξ-His tag was cleaved for 16 h at 4 °C using 1U of thrombin per 100 µg of total protein (GE Healthcare). The cleaved proteins were then passed through a Ni-NTA column to remove the cleaved 6ξ-His tag and any uncleaved protein ...
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bioRxiv - Immunology 2024Quote: ... Human serum samples were heat inactivated at 56°C for 1 h and then incubated with an equal volume of Protein G resin (GE Healthcare) for 3 nights at 4°C on a rocking platform ...
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bioRxiv - Immunology 2024Quote: ... NHP serum samples were heat inactivated at 56°C for 1 h and then incubated with an equal volume of Protein A resin (GE Healthcare) for 3 nights at 4°C on a rocking platform ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were clarified by centrifugation at 50,000⍰×⍰g for 1⍰h at 4⍰°C and the supernatant was used for affinity purification on HisTrap FF columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... cleared extracts were alkylated twice with 20 mM iodoacetamide (3 h at RT in the dark) and then cleaned by two passages on PD-SpinTrap G-25 columns (GE Healthcare). Samples were then clicked as explained before ...
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bioRxiv - Biochemistry 2020Quote: ... for 1 h at 37 °C and the RNA was desalted twice by Illustra Microspin G-25 columns (GE Healthcare Life Sciences). Method for EMSA was modified from a previously published protocol (30) ...
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bioRxiv - Molecular Biology 2020Quote: ... with Tris-buffered saline/Tween-20 was followed by incubation for 1 h at room temperature with the appropriate secondary antibody (Anti-mouse IgG horseradish peroxidase cat# NA931V from GE Healthcare, Uk). Western blots were developed using the ECL Prime western blotting detection Reagent (RPN 2232 ...
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bioRxiv - Bioengineering 2021Quote: ... at 25 mA for 1 h and transferred onto an Amersham™ Protran® 0.2 μm nitrocellulose blotting membrane (GE Healthcare, Canada) at 300 mA for 1.5 h ...
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bioRxiv - Cell Biology 2021Quote: ... human ECs were transfected twice (at 0 and 24 h) with 200 pmol of siGENOME Non-Targeting siRNA #1 (as control) or siGENOME SMART pools siRNA oligonucleotides (GE Healthcare Dharmacon), using Oligofectamine Transfection Reagent (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2021Quote: ... The suspension was incubated up to 1 h and the non-coupled free dye was removed by exclusion chromatography using a PD10 exclusion column (GE Healthcare, Sweden) equilibrated with 50 mM NaH2PO4/Na2HPO4 with 1% glycerol.
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bioRxiv - Cell Biology 2021Quote: ... HRP-conjugated secondary antibodies were incubated for 1 h at room temperature and membrane development was performed using Enhanced Chemiluminescence (ECL) Western Blotting substrate (GE healthcare, RPN2106) or ECL Prime Western Blotting substrate (GE healthcare ...
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bioRxiv - Developmental Biology 2021Quote: ... The immunoblots were washed and incubated for 1 h at RT with HRP-linked secondary antibody donkey anti-rabbit IgG (GE Healthcare, NA934). The HRP-signal was detected using SuperSignalTM West Pico PLUS Chemiluminescent Substrate Kit (Thermo Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... The following secondary antibodies coupled to horseradish peroxidase (HRP) were applied for 1 h: sheep anti-mouse (#NA9310V, GE Healthcare, 1:4000), donkey anti-rabbit (#NA9340V ...
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bioRxiv - Plant Biology 2022Quote: ... induced for 3 h induction by IPTG (isopropyl b-D-thiogalactopyranoside) and soluble extracts were purified using GST-sepharose 4B (GE Healthcare, Sweden) as described elsewhere (Geisler et al. ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then collected by vacuum filtration onto glass microfiber filters (grade GF/C, 125 mm in diameter, pre-combusted at 460°C for > 5 h, GE Healthcare Whatman). The filters were transferred to 50 mL centrifuge tubes (Sarstedt ...
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bioRxiv - Microbiology 2022Quote: ... were collected by gentle vacuum filtration onto glass microfiber filters (grade GF/A, 47 mm in diameter, pre-combusted at 460°C for > 5 h, GE Healthcare Whatman), immediately plunged into liquid nitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then collected by gentle vacuum filtration of 1-2 L onto glass microfiber filters (grade GF/A, 47 mm in diameter, pre-combusted at 460°C for > 5 h, GE Healthcare Whatman). The filters were transferred to 2.0 mL centrifuge tubes (SafeLock ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins in the strip were separated by SDS-PAGE (200 V, 5 h) and the gel was imaged using a Typhoon 9500 fluorescence imager (GE Healthcare, Germany) at a resolution of 100 μm/pixel for Cy2 at 488 nm excitation ...
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bioRxiv - Cell Biology 2022Quote: ... Extract was cleared by centrifugation at 186.000 x g for 1 h at 4°C and then incubated at 4°C with Glutathione Sepharose™ 4B resin (GE Healthcare) for 3h ...
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bioRxiv - Neuroscience 2022Quote: ... Images were captured one frame every 1 min 30 sec over 5 h using a DeltaVision Imaging System (Applied Precision, Bratislava, Slovakia).
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was washed 5 times in PBS-T before it was incubated with the secondary antibody goat α-mouse IgG (H&L)-HRP (1:10’000, GE healthcare #NXA931). After 1 h incubation ...
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bioRxiv - Molecular Biology 2023Quote: ... 40 W for 2–2.5 h) followed by detection on an Amersham Typhoon Gel and Blot Imaging System (GE Healthcare Life Sciences).
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bioRxiv - Molecular Biology 2024Quote: ... the gel was exposed to phosphor imager screen (Fujifilm Bas cassette2, Japan) for 4 h followed by imaging with Typhoon 9210 phosphor imager (GE Healthcare, USA) and Azure Sapphire (Azure Biosystems ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were washed 3 times for 5 min in TBST before incubation for 1 h at room temperature with secondary antibodies: donkey anti-mouse (1:5000; GE Healthcare, NA931-1ML), or donkey anti-rabbit (1:5000 ...
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bioRxiv - Plant Biology 2021Quote: ... Radioactive gels were exposed to a BAS phosphor-imaging plate (TR2040, Fuji) for 24 h and visualised using a Typhoon scanner (GE Healthcare, FLA-9500).