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Citations for GE Life Sciences :
51 - 100 of 231 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... Supernatants were incubated for 2 h with Glutathione Sepharose® 4 Fast Flow Beads (GE Healthcare) while rotating ...
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bioRxiv - Cell Biology 2024Quote: ... at 37 °C for 1 h and alkylated with 15 mM IAA (GE Healthcare, Mississauga, ON) in the dark at room temperature for 30 min followed by quenching with 15 mM DTT ...
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bioRxiv - Neuroscience 2020Quote: ... the membrane was incubated for 1□h at room temperature with anti-mouse (1:5000, GE Healthcare), anti-rabbit (1:5000 ...
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bioRxiv - Synthetic Biology 2021Quote: ... 2 mM 2-mercaptoethanol for 12 h and purified by anion exchange chromatography (HiTrap Q, GE Healthcare) with a linear gradient of 0 to 500 mM NaCl over 40 min and size-exclusion chromatography (HiLoad 16/600 Superdex 200 ...
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bioRxiv - Microbiology 2021Quote: ... Eranthie Weerapana) for 1 h at 22 °C and desalted (PD Miditrap G-25 columns, GE Healthcare). Each light lysate was then combined 1:1 with a heavy lysate in 1 mL total volume and incubated with 2% SDS and 2 M urea final concentration in PBS in a final volume of 2 mL for 40 min at 22 °C ...
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bioRxiv - Biochemistry 2022Quote: ... The eluent was bound to glutathione sepharose beads (10 mL, GE Healthcare Life Sciences, USA, 6 h), washed with 1 L wash buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Supernatant was incubated (2 h, 4 °C) with 4.0 mL of Glutathione Sepharose 4B resin (GE Healthcare) that had been pre-equilibrated in the same buffer ...
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bioRxiv - Zoology 2020Quote: ... and 3500 V/2 mA/5 W for 6 h using a Multiphor II system (GE Healthcare). The IEF strip was then equilibrated for 15 min in 5 ml equilibration stock solution (6 M urea ...
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bioRxiv - Genetics 2021Quote: ... incubated for 1 h at 65°C and dot-blotted on Amersham HybondTM-XL membrane (GE Healthcare). DNA was fixed by UV using a UV crosslinker BLX-254 (Analytik Jena) ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag was cleaved for 16 h at 4°C using 1U of thrombin (GE Healthcare) per 100 μg of total protein ...
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bioRxiv - Microbiology 2024Quote: ... Incubation was carried out for 24 h before imaging plates on Typhoon FLA 9500 scanner (GE healthcare) using the DY-520XL filter setting ...
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bioRxiv - Molecular Biology 2022Quote: ... the membrane was incubated for 1 h at room temperature with anti-mouse (1:5000, GE Healthcare), anti-rabbit (1:5000 ...
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bioRxiv - Plant Biology 2023Quote: ... a Sephadex LH-20 chromatographic column (r=1.2 cm × h=30 cm; GE Healthcare Bio-Sciences AB) was prepared by packing powder into a glass column and washed to remove contaminants with an elution buffer consisting of 1% HCl in 75% ethanol ...
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bioRxiv - Molecular Biology 2022Quote: ... Precipitated H-NS was dissolved in nickel binding buffer and applied to a HisTrap Ni2+ column (GE Healthcare). Fractions containing H-NS were mixed with TEV protease to remove the N-terminus His6x-tag ...
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bioRxiv - Biochemistry 2021Quote: ... GST-TEV-FICD was then depleted by a 1 h incubation with GSH-Sepharose 4B matrix (GE Healthcare). AMPylation was confirmed as being stoichiometric by intact-protein mass spectrometry (LC-ESI-MS ...
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bioRxiv - Biochemistry 2021Quote: ... dried for 2 h at 65°C and radiolabelled products visualised using a Typhoon FLA-700 (GE Healthcare) following exposure to a phosphorimaging plate for 24-72 h.
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bioRxiv - Biochemistry 2022Quote: ... The resulting supernatant was incubated for 4-5 h at 4 °C on glutathione sepharose 4B (GE Healthcare) (3 mL per 2 L expression) ...
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bioRxiv - Zoology 2020Quote: ... for 1 h at room temperature and developed in ECL (Amersham ECL Select; GE Healthcare, Chicago, IL, USA). Bands were visualized with the MultiImage III FluorChem Q (Alpha Innotech ...
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bioRxiv - Microbiology 2021Quote: Method 1 (H-P): Genomiphi™ V2 DNA Amplification Kit (Cytiva illustra™, formerly GE Healthcare Life Sciences) was used for whole genome amplification according to manufacture instruction ...
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bioRxiv - Biochemistry 2020Quote: ... FIH was induced for 16 h at 16°C and purified using a MPBTrap HP column (GE Healthcare). Proteins were exchanged into 150 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... CAD was induced for 8 h at 30°C and purified using a HisTrap HP column (GE Healthcare). FIH was induced for 16 h at 16°C and purified using a MPBTrap HP column (GE Healthcare) ...
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bioRxiv - Physiology 2023Quote: ... and subsequently probed with secondary anti-mouse/-rabbit IgG (H+L)-HRP conjugates (1:6000, GE Healthcare, USA). Quantification was carried out on original scanned images using Image Lab software (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... Images were acquired every 10 min for 12 h using an IN Cell Analyzer 2000 microscope (GE Healthcare; Nikon 20x/0.45 NA Plan Fluor objective ...
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bioRxiv - Molecular Biology 2023Quote: ... treated 1 h before filming with siRDNA (1:1000) and imaged using a Deltavision Core system (Applied Precision).
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bioRxiv - Genetics 2020Quote: ... Three washes were performed with TBST followed by 1 h incubation with secondary antibody (ECL, HRP linked, GE Healthcare) and another series of washes ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were then blocked for 1 h with TBS-T 1% ECL Prime blocking reagent (GE Healthcare cat # RPN418) at RT on an orbital shaker and blotted with primary antibody for 1 h at RT with gentle agitation ...
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bioRxiv - Molecular Biology 2020Quote: ... Reactions were incubated at 37 °C for 1 h and then passed through G-25 spin columns (GE Healthcare) according to the manufacturer’s instructions to remove unincorporated radioactivity ...
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bioRxiv - Biophysics 2021Quote: Protein samples were exchanged into a H-buffer using a PD-10 G-25 media desalting column (GE Healthcare) to induce droplet formation ...
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bioRxiv - Cell Biology 2022Quote: ... The extracted mononucleosome fraction was incubated for 2 h at 4°C with Protein G Sepharose beads (GE Healthcare), which were preincubated with rabbit polyclonal anti–chicken CENP-A antibody64 ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 N NaOH) and 2 h with transfer buffer (1.5 M NaCl, 0.25 N NaOH) using a VacuGene XL (GE Healthcare). The DNA was fixed to the membrane by soaking it in freshly prepared 0.4 N NaOH for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... the hexa-histidine tagged H-Rrm4-M protein was purified using Nickel-based affinity chromatography (HisTrap HP, GE Healthcare) on Akta primeplus FPLC system (GE Healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were incubated at 37 °C for 1 h and then passed through G-25 spin columns (GE Healthcare) according to the manufacturer’s instructions to remove unincorporated radioactivity ...
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bioRxiv - Immunology 2024Quote: ... the Endo H was removed on a Superdex™ 200 Increase 10/300 GL size exclusion column (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... the membranes were incubated for 1 h with an anti-rabbit secondary antibody conjugated to horseradish peroxidase (GE Healthcare), diluted 1/10,000 in PBST ...
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bioRxiv - Microbiology 2022Quote: ... the membranes were incubated for 1 h with an anti-rabbit secondary antibody conjugated to horseradish peroxidase (GE Healthcare), diluted 1/10,000 in PBST ...
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bioRxiv - Cell Biology 2023Quote: ... The eluted protein was concentrated and incubated with the antibody fragment scFv16 for 2 h on ice at a molar ratio of 1:1.5.48 A Superdex 200 Increase 10/300 column (GE Healthcare) was pre-equilibrated with buffer [20 mM HEPES (pH 7.5) ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 3 h with HRP-streptavidin in TBS/10% FBS (1:200; GE Healthcare Life Sciences). After a final wash in TBS (3 x 10 min) ...
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bioRxiv - Physiology 2024Quote: ... Lysates were cleared by centrifugation (10,000 × g, 1 h) and loaded onto 5 mL Histrap FF columns (GE Healthcare). After washing column with lysis buffer supplemented 5 mM imidazole ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were incubated with HRP-conjugated secondary anitbodies at 1:3,000 for 1 h at room temperature and bands developed using ECL Prime Western Blotting Detection Reagent (GE Healthcare). Blots were subsequently incubated with 1:20,000 HRP-conjugated β-actin (Abcam ...
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bioRxiv - Cell Biology 2020Quote: ... images were obtained every 5 min for 4 h using a 20X objective (0.75 NA, PlanApo) on a DeltaVision microscope (GE Healthcare) equipped with a CoolSNAP HQCCD camera (Photometrics ...
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bioRxiv - Physiology 2022Quote: ... Supernatants were collected after centrifugation at 13,200 rpm for 15 min and precleaned for 1 h with 50% protein G-Sepharose (GE Healthcare), followed by incubation with an indicated antibody at 4 °C Upon addition of 100 μl of 50% protein G-Sepharose ...
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bioRxiv - Microbiology 2020Quote: ... The lysate was centrifuged for 1 h at 95,000g and the supernatant was loaded onto a Glutathione Sepharose HP (GE Healthcare) column ...
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bioRxiv - Biochemistry 2020Quote: ... Medium was collected 48-60 h post infection and incubated with 3.0 mL of Ni-Sepharose Excel (Cat 17-3712-03, GE Healthcare) pre-equilibrated with Buffer A (150 mM NaCl ...
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bioRxiv - Neuroscience 2020Quote: ... washed three times with 0.1 M cacodylate and then incubated for 3-h at RT in streptavidin HRP conjugate (1:200; GE Healthcare). Sections were visualized after 1-min and 30-sec DAB (Sigma-Aldrich)/glucose oxidase reaction ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was incubated at 4°C for 1 h with Ni-charged IMAC Sepharose 6 Fast Flow (GE Healthcare). The resin was then washed three times with His-A buffer (lysis buffer + 20 mM imidazole) ...
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bioRxiv - Biochemistry 2020Quote: ... The co-expressed complex of ctCyclin H and ctMAT1short was loaded on a 5 ml StrepTrap HP column (GE Healthcare) equilibrated in lysis buffer B (20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... The lysate was cleared by centrifugation and the supernatant (SN) incubated for 1.5 h with Glutathione Sepharose 4B (GE Healthcare), equilibrated in buffer A (25 mM HEPES pH 7.4 ...
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bioRxiv - Biophysics 2022Quote: ... The lysate was cleared by centrifugation and the supernatant (SN) incubated for 1.5 h with Glutathione Sepharose 4B (GE Healthcare), equilibrated in buffer A (25 mM HEPES pH 7.4 ...
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bioRxiv - Cell Biology 2020Quote: ... and within 1 h after taking out of the incubator on a commercial structured illumination microscope (DeltaVision|OMXv4.0 BLAZE, GE Healthcare). All the images were acquired using 488 nm laser with power set to either 10% or 31.5% ...
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bioRxiv - Biochemistry 2022Quote: ... The suspension was then centrifuged for 1 h at 16,000 g at 4 °C and the supernatant was added to 1 mL of glutathione beads (GE Healthcare) equilibrated in PBS ...