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Citations for GE Life Sciences :
151 - 197 of 197 citations for Dnase Free and Rnase Free Distilled Water since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Lake water was firstly filtered through a filter paper (Whatman, GE Healthcare, NY, USA) to remove large particles before filtering through 0.22 μm PES (Polyethersulfone ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing RNase E were pooled and loaded on a butyl sepharose HP column (GE Healthcare) which previously was equilibrated in high-salt buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... packed in water in a 5 mm inner diameter Tricon 5/100 Column (GE Healthcare, USA). Packing occurred by combined sedimentation and a steady flow of 100 µl/min through the column ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA were extracted using the Illustra RNAspin kit and treated with DNase 1 (GE Healthcare). cDNA synthesis and taqman qPCR were performed as previously described [16] ...
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bioRxiv - Neuroscience 2024Quote: ... The reduced 5′ thiol-modified ssDNA was purified into water using NAP-5 columns (GE Life Sciences). The reduced 5’ thiol-modified ssDNA was spin vacuum dried ...
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bioRxiv - Biochemistry 2024Quote: ... Radiolabeled oligo was then buffer exchanged into water using a Microspin G-25 spin column (GE Healthcare).
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bioRxiv - Bioengineering 2021Quote: The purified protein was desalted in deionized Milli-Q water using a PD-10 column from GE healthcare and concentrated fivefold using a Thermo scientific three kDa MWCO protein concentrator ...
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bioRxiv - Neuroscience 2020Quote: ... re-suspended in ultrapure water and pre-cleared with recombinant protein A sepharose (GE Healthcare, Chicago, IL, USA). Immunoprecipitation of Aβ species from the media was performed with the monoclonal anti-Aβ clone W0-2 (MABN10 ...
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bioRxiv - Microbiology 2020Quote: ... the membranes were immersed in ultrapure water and fluorescent signal was detected using the Typhoon system (GE healthcare). Densitometric quantification of bands was performed using ImageJ software (NIH ...
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bioRxiv - Microbiology 2020Quote: ... Duplicate 120 ml water samples were filtered serially through 2.7 μm Whatman GF/D (GE Healthcare, Little Chalfort, UK) and 0.22 μm Sterivex (Millipore ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were treated with RNase I (5U/OD260) and ribosome-protected fragments were isolated via centrifugation through Microspin S-400 HR columns (GE Healthcare) and purified using the RNA Clean and Concentrator kit (Zymo Research) ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein quality was assessed by SDS-PAGE and fractions containing RNase E were pooled and loaded onto a butyl sepharose column (GE Healthcare) equilibrated in high-salt buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Plant Biology 2023Quote: ... The DNase I-treated RNAs were reverse transcribed using Ready-to-Go RT-PCR beads (GE Healthcare Life Science) and random oligomers ...
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bioRxiv - Microbiology 2022Quote: ... antibodies diluted 1:500 in 1 U/mL DNAse I in Tris Buffered Saline containing 1% BSA (GE Healthcare). Three 5-minute washes with block (1% BSA with 0.1% Tween-20 ...
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bioRxiv - Biophysics 2020Quote: ... The purity and identity of the product was established by HPLC and mass spectrometry (BioSuite pPhenyl 1000RPC 2.0 x 75 mm coupled to a LCT-Premier Waters from GE Healthcare). Neutravidin was from Thermofisher ...
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bioRxiv - Microbiology 2020Quote: ... The gels were destained with ultra-pure water for 20 minutes and were transferred to Hydrobond-XL-membrane (GE healthcare) and electro transferred using 0.5X TAE as the transfer buffer for 45 minutes at 150 V ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... eight larvae and 300µL of egg water per well were distributed into square 96-well plates (GE Healthcare Life Sciences) using a p1000 pipette as described29 ...
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bioRxiv - Plant Biology 2023Quote: ... The gel was washed three times with Milli-Q water and scanned for fluorescence with the Typhoon scanner (GE Healthcare) using a Cy3 setting ...
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bioRxiv - Biochemistry 2020Quote: ... and Waters 410 differential refractometer (Waters) in line with an ÄKTA pure chromatography system fitted with a Superdex 200 Increase column (both GE Healthcare). Data was analysed using ASTRA 5.3.4 software (Wyatt Technology).
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bioRxiv - Biophysics 2021Quote: ... Digested peptides were separated from the solution and retained by a solid phase extraction system (SepPak, Waters) and subsequently enriched by SEC on an ÄKTAmicro chromatography system (GE Healthcare) using a Superdex™ Peptide 3.2/300 column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... SDS-gels were stained by “Blue-Silver” Coomassie Staining (Candiano et al., 2004) and destained in water overnight hours or overnight before imaging with LAS4000 (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... SDS-gels were stained by “Blue-Silver” Coomassie Staining (Candiano et al., 2004) and washed with water for 6 hours or overnight before imaging with LAS4000 (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... SDS-gels were stained by “Blue-Silver” Coomassie Staining (Candiano et al, 2004) and washed with water for 6 hours or overnight before imaging with LAS4000 (GE Healthcare).
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bioRxiv - Synthetic Biology 2020Quote: ... The reaction was quenched by the addition of 20 μl water followed by desalting in a MicroSpin G-25 spin column (GE Healthcare). The ∼53 μl eluate was transferred to a PCR tube and placed under a 385 nm UV lamp (∼3 cm distance from the tops of the tubes ...
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bioRxiv - Biochemistry 2021Quote: ... A 30 μl aliquot of a given reaction was quenched by the addition of 20 μl water followed by desalting in a MicroSpin G-25 spin column (GE Healthcare). The NPOM (caged ...
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bioRxiv - Microbiology 2022Quote: ... SDS-gels were stained by “Blue-Silver” Coomassie Staining (Candiano et al., 2004) and washed with water for 6 hours or overnight before imaging with LAS4000 (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... DSPE-PEG- OCH3 (DSPE-mPEG) and DSPE-PEG-S2P were dissolved in HyPure water (GE Healthcare Life Sciences, catalog no. SH30538) at the concentration of 0.1 mg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... DSPE-PEG-OCH3 (DSPE-mPEG) and DSPE-PEG-GalNAc were dissolved in HyPure water (GE Healthcare Life Sciences, catalog no. SH30538) at the concentration of 0.1 mg/mL ...
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bioRxiv - Biochemistry 2021Quote: Single cysteine mutants of ColE9 or the ColE9 DNase domain were desalted on a 5 ml HiTrap column (GE Healthcare) equilibrated with binding buffer (20 mM potassium phosphate pH 7 ...
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bioRxiv - Plant Biology 2021Quote: ... Germany) following manufacturer’s instructions and contaminant genomic DNA was removed using 10 U of DNase I (GE Healthcare, Munich, Germany). The quantity and the quality of RNA was checked using a spectrophotometer ...
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bioRxiv - Systems Biology 2021Quote: ... the gels are washed with deionized water and exposed on a phosphorscreen (Fujifilm, Japan) followed by imaging with Typhoon phosphorimager (GE Healthcare, USA). Semi-quantitative densitometric analysis of the autoradiograms was done using ImageJ software.
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bioRxiv - Microbiology 2020Quote: ... Supernatants were up concentrated by rotary evaporation and disaccharides were desalted in ultrapure water (milliQ) using a HiPrep Desalt column (GE Healthcare, Denmark) on an Äkta avant chromatograph (GE Healthcare) ...
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bioRxiv - Biochemistry 2024Quote: ... n)18F nuclear reaction through bombardment of enriched [18O]water with 16 MeV protons at 65 µA for 6 min using a PETtrace cyclotron from GE Healthcare (GEHC) at Massachusetts General Hospital ...
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bioRxiv - Molecular Biology 2023Quote: ... T7 RNAP-based in vitro transcription was performed as described previously.10 The produced λtR1 rut site RNA was treated with DNase I and purified by strong anion-exchange (MonoQ, GE Healthcare) and size-exclusion chromatography (Superdex 75 ...
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bioRxiv - Molecular Biology 2020Quote: ... The collected water samples were immediately filtered using glass fibre filters with a mesh size of 0.7 μm (GF/F, GE Healthcare Japan, Tokyo, Japan) and stored at −20°C until DNA extraction ...
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bioRxiv - Neuroscience 2021Quote: ... n)18F reaction by irradiating 18O-enriched water (Isoflex Isotope, San Francisco, CA) in a GE PETtrace 16.5 MeV cyclotron (GE Healthcare, Waukesha, WI, USA). The [18F]fluoride solution was enriched with a QMA Sep-Pak Cartridge (Sep-Pak plus light ...
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bioRxiv - Molecular Biology 2020Quote: ... one of which was heating in a 65 °C water bath for 60 minutes and the second was using a Hi Trap QHP column (1 mL GE Healthcare, United States). When loading ...
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bioRxiv - Bioengineering 2023Quote: ... Released mesh probes were rinsed with deionized (DI) water three times and transferred to sterile 1× PBS solution (HyClone, GE Healthcare Life Sciences) before surface modification or implantation.
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bioRxiv - Biochemistry 2022Quote: ... SEC-HPLC was carried out using an Alliance e2695 HPLC system (Waters Co., Milford, MA, USA) equipped with a Superose 6 Increase 10/300 GL column (GE Healthcare Life Sciences) and a 2998 photodiode array (PDA ...
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bioRxiv - Cell Biology 2021Quote: ... Gels were incubated in Bjerrum Schafer-Nielsen transfer buffer and proteins were transferred to a water-activated nitrocellulose membranes (Amersham Protran Premium 0.45 NC, GE Healthcare Life Sciences, Sunnyvale, USA) using semi-dry transfer (Trans-Blot® Turbo™ ...
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bioRxiv - Cell Biology 2020Quote: ... Gels were incubated in Bjerrum Schafer-Nielsen transfer buffer and proteins were transferred to a water-activated nitrocellulose membranes (Amersham Protran Premium 0.45 NC, GE Healthcare Life Sciences, Sunnyvale, USA) using semi-dry transfer (Trans-Blot® Turbo™ ...
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bioRxiv - Plant Biology 2020Quote: ... plant roots (pool of three root systems per FlowPot) were thoroughly washed with sterile Milli-Q water and dried out with sterilized Whatman glass microfiber filters (GE Healthcare Life Sciences, USA). Matrix samples ...
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bioRxiv - Plant Biology 2020Quote: 9 mL of blocked protein sample was combined with 4.5 mL of either 1M hydroxylamine (final concentration 333 mM) or water and 80 mg (dry weight equivalent) thiopropyl sepharose beads (GE Healthcare Catalog number 17042001) and incubated for 1 hour at room temperature in a column fitted with a porous disc ...
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bioRxiv - Neuroscience 2021Quote: ... and proteins were transferred to an activated (100% Ethanol, 1 min; followed by two washing steps with water) PVDF membrane (GE Healthcare, Buckinghamshire, UK; Cat# 10600023) at 20V for 45 min ...
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bioRxiv - Biochemistry 2020Quote: ... 150 μl of DEPC-treated water was then added and the reaction passed through a home-poured G-25 sephadex (GE Healthcare cat. # 17-0033-01) spin column ...
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bioRxiv - Biochemistry 2022Quote: ... the peptides were passively eluted into 72 contiguous fractions with MilliQ water/ 35% acetonitrile (CAN)/ 35% ACN + 0.1% formic acid (FA) using an in-house constructed IPG extraction robot (GE Healthcare Bio-Sciences AB, prototype instrument) into a 96-well plate (V-bottom ...
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bioRxiv - Biophysics 2022Quote: ... reactions were diluted with water (970 μl) and purified by desalting on a disposable gel-filtration column (NAP10, GE Healthcare Life Sciences, cat. no. GE17-0854-02) according to the manufacturer’s instruction to remove the majority of the free dye ...