Labshake search
Citations for GE Life Sciences :
101 - 150 of 197 citations for Dnase Free and Rnase Free Distilled Water since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... Cells were washed with cold phenol red-free DMEM with 10% FBS and 20 mM HEPES (GE Healthcare Life Sciences) to remove unbound viruses and incubated at 37 °C to initiate virus-cell fusion ...
-
bioRxiv - Immunology 2022Quote: ... Conjugated SOSIP-mi3 particles were separated from free SOSIPs by SEC on a Superose 6 10/300 column (GE Healthcare) equilibrated with PBS ...
-
bioRxiv - Microbiology 2020Quote: ... HyClone water (GE Healthcare) was used throughout.
-
bioRxiv - Biochemistry 2020Quote: The recombinant catalytic domains of MMP-2 and -9 were expressed in HEK293 cells stably transfected with respective constructs and purified from serum-free culture medium using Gelatin Sepharose 4B (GE Healthcare) as described in [35 ...
-
bioRxiv - Biochemistry 2021Quote: ... Gels were dried and radioactivity in bound and free bands were quantified using a Phosphorimager (GE) and ImageQuant 5.2 software (GE Healthcare, IL). Plots of the fraction bound RNA vs ...
-
bioRxiv - Biochemistry 2020Quote: ... Labeling of SNAP fusion proteins and depletion of free dye was confirmed by SDS-PAGE followed by fluorescence imaging on a Typhoon imager (GE Healthcare). Extracts were checked to verify transcription activity (e.g. ...
-
bioRxiv - Biochemistry 2020Quote: ... The 8 × His tag was removed by TEV protease and tag-free WDR522-334 was further purified by gel filtration using a Superdex 75 (10/300) increase column (GE Healthcare) in 20 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2020Quote: ... 10.2 µM protein was incubated with 100 µM probe and 0.5 mM TCEP for ∼20 h at 4 °C and free probe was removed by desalting using a 5 mL HiTrap desalting column (GE Healthcare).
-
bioRxiv - Plant Biology 2021Quote: ... The GST tag was cleaved from purified protein by elution of the tag-free protein from the column by overnight incubation with PreScission Protease (GE Healthcare) or alternatively by elution with 15 mM glutathione-containing purification buffer ...
-
bioRxiv - Microbiology 2021Quote: ... The eluted protein was buffered exchanged into endotoxin-free phosphate buffered saline (PBS) using a sterile PD MidiTrap G-25 column (GE Healthcare). Finally ...
-
bioRxiv - Biophysics 2022Quote: ... After that the labeled protein was purified from the excess of free dye by a PD SpinTrap G-25 column (GE Healthcare), divided into aliquots and stored at -80 °C.
-
bioRxiv - Biochemistry 2022Quote: ... Proteins were then passed through Glutathione Sepharose resin to remove the free GST and concentrated to run through Superdex-75 (GE Healthcare) into 200 mM NaCl ...
-
bioRxiv - Immunology 2020Quote: ... Recombinant IgG antibodies were purified from cell-free supernatants by affinity purification using Protein G or Protein A sepharose beads (GE Healthcare). Purified proteins were dialyzed in PBS ...
-
bioRxiv - Microbiology 2022Quote: ... The free fluorescent probe was removed by a step of gel filtration chromatography (Superdex 200 Increase 10/300 GL; GE Healthcare) in the reaction buffer 50mM Tris (pH7.5) ...
-
bioRxiv - Biochemistry 2020Quote: ... The reaction was quenched with 10 mM DTT and free dye was separated by size exclusion chromatography on a HiLoad Superdex 200 16/60 column (GE Healthcare). Fractions contained labelled protein were pooled and concentrated with Amicon Ultra-15 50 kDa cutoff centrifugal filter units (Millipore) ...
-
bioRxiv - Immunology 2020Quote: ... Conjugated VLPs or NPs were then separated from free HA trimers by SEC on a Superose 6 10/300 (GE Healthcare) column equilibrated with PBS (20 mM sodium phosphate pH 7.5 ...
-
bioRxiv - Biochemistry 2021Quote: ... The click-reaction was performed by incubation for 30 min at 20 °C in the dark with vigorous intermittent shaking and the free biotin was removed from the clicked extracts using PD MiniTrap G-25 column (GE Healthcare) equilibrated with IPD buffer ...
-
bioRxiv - Neuroscience 2021Quote: ... and AAV2-CMV-mKO) were produced using a helper-free triple transfection procedure and purified via affinity chromatography (GE Healthcare, USA).
-
bioRxiv - Microbiology 2022Quote: ... end-labelled with [γ-32P ATP] (1000 Ci nmol-1) using T4 polynucleotide kinase and purified from free label by Sephadex G-50 spin columns (GE Healthcare). Increasing amounts of purified regulators were incubated with appropriately end-labelled DNA probes in a total volume of 10 µl binding mix (50 mM Tris-HCl ...
-
bioRxiv - Biophysics 2022Quote: ... Broken cells then underwent ultra-centrifugation at 142,000 x g for 60 minutes before collecting the cell free extract and incubating it with pre-equilibrated HisTrap Ni2+ resin (GE Healthcare) in a 3:1 volume ratio for 2 hours under gentle shaking ...
-
bioRxiv - Biochemistry 2023Quote: ... The flow through (containing soluble free histones) was filtered with 0.22 μ filters and loaded on a cation exchange Resource S column (GE Healthcare) equilibrated with the dilution buffer EB5_1 (50 mM Tris-HCl pH8) ...
-
bioRxiv - Cell Biology 2022Quote: ... The mTORC1 complex and free RAPTOR were separated by anion exchange chromatography on a 5 mL HiTrap Q column (GE Healthcare). The fractions containing mTORC1 complex and free Raptor were concentrated to 1.3 mg/mL and 0.5 mg/ml ...
-
bioRxiv - Cell Biology 2022Quote: ... The tag-free protein was further purified by size-exclusion chromatography on a Hiload 16/60 Superdex 75 column (GE Healthcare). Mutants and ZF truncations were expressed and purified using the same procedure as for the wild-type proteins ...
-
bioRxiv - Microbiology 2024Quote: ... Protein samples were resolved by stain-free sodium dodecyl sulfate–10% polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a nitrocellulose membrane (GE Healthcare) using the semi-dry Trans-Blot turbo transfer system (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cyclin E was induced by washing cells three times in PBS and transferring into medium containing 10% tetracycline-free fetal calf serum (PAA-GE Healthcare), G418 and puromycin ...
-
bioRxiv - Biochemistry 2024Quote: ... The His-tag free MUC5AC-D3 variants were loaded onto a HiPrep Q HP 16/10 anion exchange chromatography column (GE Healthcare) and eluted in a 0-500 mM NaCl gradient in 20 mM Tris pH 8.
-
bioRxiv - Cell Biology 2024Quote: Cell-free extracts from Drosophila heads were incubated with a 50% slurry of equilibrated Protein A-Sepharose beads (GE Healthcare Ltd) in the ratio of 100 µl bead slurry for 1 ml extract for the Pre –clearing step in Immunoprecipitation ...
-
bioRxiv - Neuroscience 2021Quote: ... Free calcein or SST was removed by size-exclusion chromatography with Sephacryl® S-1000 column (Cat. # 17-0476-01, GE Healthcare). Second ...
-
bioRxiv - Microbiology 2020Quote: ... The labeled protein was separated from the excess of free 125I in a PD10 desalting column (GE Healthcare Life Sciences, United Kingdom) and the purity of the 125I-labeled Vip3Aa was checked by autoradiography ...
-
bioRxiv - Biophysics 2021Quote: ... The suspension was incubated up to 1 h and the non-coupled free dye was removed by exclusion chromatography using a PD10 exclusion column (GE Healthcare, Sweden) equilibrated with 50 mM NaH2PO4/Na2HPO4 with 1% glycerol.
-
bioRxiv - Biochemistry 2023Quote: ... The cleaved His6-SUMO tag and uncleaved His6-SUMO-GBP2 were separated from tag free GBP2 by passing the solution over a His-Trap column (GE Healthcare/Cytiva). Tag free GBP2 in the flow-through was concentrated and subjected to size exclusion chromatography on a HiLoad16/600 Superdex 200pg column (GE Healthcare/Cytiva ...
-
bioRxiv - Cell Biology 2023Quote: ... Labeled protein was separated from free fluorescent dye using a gel filtration column (GE Healthcare illustra NAP-10 Columns Sephadex G-25), followed by ESI-MS analysis (Agilent 6230 TOF LC/MS ...
-
bioRxiv - Neuroscience 2023Quote: ... The adeno-associated virus (AAV) vectors were produced by helper-free triple transfection and purified using affinity chromatography (GE Healthcare, Chicago, IL, USA). For production of AAV2.1 vector ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... The dye-labeled protein was purified from unreacted free dye using a PD-10 column containing Sephadex G-25 Medium (GE Healthcare Life Sciences) and then centrifuged at 50,000 rpm for 30 min ...
-
bioRxiv - Molecular Biology 2024Quote: ... Recombinant single-stranded rAAV2/8 was produced using an adenovirus-free triple transfection method of HEK293 and purified by single affinity chromatography (AVB Sepharose; GE Healthcare, Chicago, IL). The final product was formulated in sterile phosphate buffered saline containing 0.001% of Pluronic (F68 ...
-
bioRxiv - Neuroscience 2021Quote: ... The α-syn oligomers and free α-syn from the supernatant were separated using a Superdex 200 Increase 10/300 GL (GE Healthcare, 28990944) equilibrated with PBS ...
-
bioRxiv - Physiology 2020Quote: ... U2OS-ERα cells were cultured in phenol-free αMEM medium supplemented with 10% HyCloneTM charcoal/dextran stripped FBS (GE Healthcare Life Sciences, Pittsburgh, PA), 1% antibiotic/antimycotic ...
-
bioRxiv - Neuroscience 2022Quote: ... The labelled protein was purified from the excess of free dye by a P10 desalting column with Sephadex G25 matrix (GE Healthcare, Waukesha, WI, USA), divided into aliquots ...
-
bioRxiv - Genomics 2020Quote: ... Hyclone water (GE Healthcare Life Sciences) was used in all assays ...
-
bioRxiv - Immunology 2022Quote: ... The cell free supernatant derived during harvest of the fermented broth was processed using Blue Sepharose™ 6 Fast Flow column (GE Healthcare, Cat# 17-0948-01) in flow-through mode followed by a hydrophobic interaction chromatography (HIC ...
-
bioRxiv - Microbiology 2021Quote: ... The gel was rinsed with double distilled H2O and scanned at the TAMRA channel on a Typhoon Scanner (GE Healthcare). In an alternative procedure ...
-
bioRxiv - Biophysics 2020Quote: Proteins were finally separated from free fluorophores by size exclusion chromatography with a PD MiniTrap G-25 prepacked columns (GE Healthcare Life Science, Chalfont St Giles, UK). Finally ...
-
bioRxiv - Microbiology 2020Quote: ... cells were fixed with HyClone Water (GE Healthcare Life Science) + 2% PFA for 20 min on ice ...
-
bioRxiv - Biochemistry 2021Quote: ... RNase HI was loaded onto a 16/600 Superdex 75 column (GE Healthcare), whilst PolI and Ligase were further purified on a 16/600 Superdex 200 column (GE healthcare) ...
-
bioRxiv - Genomics 2020Quote: ... cDNA was exchanged into water using G-50 columns (GE Life Sciences) the volume increased to 68 μl using nuclease free water ...
-
bioRxiv - Cell Biology 2021Quote: ... water-swollen thiopropyl sepharose 6B beads (GE Healthcare Life Sciences, Mississauga, ON) were added and samples were incubated 2 hrs at RT on rotating wheel ...
-
bioRxiv - Cell Biology 2024Quote: ... Water-swollen thiopropyl sepharose 6B beads (GE Healthcare Life Sciences, Mississauga, ON) were added and samples were incubated 2 hrs at RT on rotating wheel ...
-
bioRxiv - Animal Behavior and Cognition 2020Quote: ... sections were washed in 4X SSC and RNA digested with RNase A (GE Healthcare) at 0.02 mg⁄mL in an appropriate buffer (0.5M NaCl ...
-
bioRxiv - Neuroscience 2022Quote: ... half-filled with deionized water and lined with filter paper (GE Healthcare, WHA1001055) were introduced to the cage as continuously available egg-laying substrates between 3 and 6 days after blood-feeding ...
-
bioRxiv - Bioengineering 2023Quote: ... we achieve this using water bottles and the Braino MRS phantom (GE Healthcare) placed in the scanner.