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Citations for GE Life Sciences :
1901 - 1950 of 4331 citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... Protein was then sequentially purified by a HiTrapQ HP anion exchange column (GE Healthcare) followed by size exclusion chromatography on a Superdex 200 Increase column in Gel Filtration Buffer supplemented with 10% v/v glycerol ...
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bioRxiv - Biophysics 2021Quote: ... The protein purified with SEC was loaded to a Strep-Trap column (GE Healthcare) and eluted using PBS buffer with 2.5 mM desthiobiotin to remove unreacted anticalin.
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were visualized using ECL Western blot reagent (GE Healthcare, Chalfont St. Giles, UK).
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bioRxiv - Cell Biology 2021Quote: GST-tagged proteins were purified using affinity chromatography with Glutathione Sepharose beads (GE Healthcare) in a cold room ...
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bioRxiv - Neuroscience 2020Quote: ... the protein was loaded onto a Superdex 200 26/600 HiLoad column (GE Healthcare) with 5 mM MOPS ...
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bioRxiv - Plant Biology 2021Quote: ... protein extracts were passed through a Sephadex G-25 column (GE Healthcare, Chicago, Illinois) and pre-cleared by treatment with rabbit-IgG agarose (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... Labelled protein was applied to a Superdex 200 10/300 GL column (GE Healthcare) equilibrated with 20 mM potassium phosphate pH 7 ...
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bioRxiv - Biochemistry 2020Quote: ... Digested protein was applied to a 5 mL HiTrap Q HP column (GE Healthcare) at 0.5 mL/min ...
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bioRxiv - Biochemistry 2020Quote: ... The cleaved protein was then loaded on a HiTrap Heparin HP column (GE Healthcare). Elution was performed by a single step using 25 mM Hepes ...
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bioRxiv - Cell Biology 2021Quote: Protein samples were separated by SDS-PAGE and transferred onto nitrocellulose membranes (GE Healthcare) using the Trans-Blot Turbo System (BioRad) ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were then blotted on a 0.45-µm pore size nitrocellulose membrane (GE Healthcare) using a wet/tank transfer blotting system (Biorad ...
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bioRxiv - Cell Biology 2021Quote: ... GST fusion proteins were purified over Glutathione (GSH) beads (GE Healthcare Biosciences, Pittsburg, PA) in PBS buffer supplemented with protease inhibitors and eluted with 20mM GSH in PBS pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... The filtrate was applied to a ÄKTA protein purification system (GE Healthcare Life Sciences), which was equipped with a His-trap column ...
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bioRxiv - Systems Biology 2021Quote: ... The proteins were separated and transferred to PVDF membrane (GE Healthcare Cat No-10600023) using semi-dry transblot apparatus (Hoefer ...
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bioRxiv - Microbiology 2020Quote: ... The eluted protein was passed through a Superose 6 column (GE Healthcare Life Sciences) using gel filtration buffer (20mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... The eluted protein was passed through a Superose 6 column (GE Healthcare Life Sciences) using gel filtration buffer (20mM HEPES ...
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bioRxiv - Microbiology 2021Quote: ... the protein was concentrated to 1 mg/ml using a vivaspin 20 (GE Healthcare) and store at −80 °C ...
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bioRxiv - Systems Biology 2021Quote: ... and vortexing before protein quantification using the 2D Quant Kit (GE Healthcare Life Sciences).
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bioRxiv - Microbiology 2020Quote: ... The fusion proteins were purified using Glutathione-Sepharose 4B (GE Healthcare, 17-0756-01) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... The concentrated protein was then loaded onto superdex75 (10/60) chromatography column (GE Healthcare) pre-equilibrated with 20 mM Tris-HCl buffer at pH 7.4 ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were dialyzed with the PD-10 Desalting Column (GE Healthcare, #GE17-0851-01) in a buffer consisting of 20 mM Tris-HCl pH=7.5 ...
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bioRxiv - Biophysics 2021Quote: ... Protein was purified with a 5-mL HiTrap SP cation-exchange column (GE Healthcare) and eluted with a salt gradient (10–1000 mM NaCl) ...
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bioRxiv - Biophysics 2020Quote: ... 6xHis-tagged PPARγ LBD protein was immobilized on an NTA sensor chip (GE Healthcare), which includes a reference flow cell for background subtraction ...
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bioRxiv - Biochemistry 2022Quote: ... Dialyzed proteins were loaded onto two 5 mL HiTrap Heparin HP columns (GE Healthcare) connected in tandem ...
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bioRxiv - Biochemistry 2022Quote: ... Dialyzed proteins were loaded onto a 5 mL HiTrap Heparin HP column (GE Healthcare) and eluted with a linear gradient of 20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... The protein was passed over a size exclusion column (Superdex 200, GE Life Sciences) in buffer GF (buffer A plus 300 mM NaCl and 1 mM dithiothreitol (DTT)) ...
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bioRxiv - Biochemistry 2022Quote: ... proteins were further purified by SEC using a Superdex 200 column (GE Healthcare, USA), in running buffer (10 mM Tris-HCl ...
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bioRxiv - Physiology 2022Quote: ... Separated proteins were transferred onto 0.2 µm Nitrocellulose membranes (catalog no 10600015, GE Healthcare) in Tris-Glycine (25 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein was purified on a Ni Sepharose 6 Fast Flow column (GE Healthcare) under gravity and eluted with the elution buffer (300 mM Imidazole ...
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bioRxiv - Microbiology 2022Quote: ... the purified protein was loaded into a HiPrep 26/10 desalting column (GE Healthcare), and the buffer was exchanged to Tris 20 mM ...
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bioRxiv - Biophysics 2022Quote: ... Fractions containing target protein were desalted from imidazole using PD10 desalting column (GE Healthcare) and incubated with 50 μM ONO-5430608 and a His-tagged PreScission protease (homemade ...
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Alpha-1-antitrypsin binds to the glucocorticoid receptor with biological significance in macrophagesbioRxiv - Immunology 2022Quote: ... Protein A Sepharose™ 4 Fast Flow sepharose beads were purchased from GE Healthcare Life Sciences (Pittsburgh ...
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bioRxiv - Microbiology 2022Quote: GST-tagged protein was purified using Glutathione-based affinity chromatography (GSTrap FF GE Healthcare). Cells were thawed on ice and resuspended in Buffer F (20 mM HEPES pH 8.0 ...
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bioRxiv - Immunology 2022Quote: ... Trimeric spike protein was further purified by Superose 6 size-exclusion chromatography (GE Healthcare) in 10 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2022Quote: ... Dialyzed proteins were loaded onto a 5 mL HiTrap Heparin HP column (GE Healthcare) and eluted with a linear gradient of 20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Cancer Biology 2022Quote: ... the proteins were loaded onto a HiLoad Superdex-S200 size-exclusion column (GE Healthcare) using AKTA purifier system (GE Healthcare) ...
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bioRxiv - Cell Biology 2022Quote: ... The GST-tagged proteins were absorbed with Glutathione-Sepharose 4B affinity matrix (GE Healthcare) for 1.5hrs and recovered by 3C protease cleavage at 4°C overnight in 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... purified proteins were passed through HiLoadTM 16/60 SuperdexTM 200pg (GE Healthcare Life Sciences). The purified protein was then stored in -80°C in buffer containing 25 mM Tris pH 8.0 ...
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bioRxiv - Neuroscience 2020Quote: ... Immunoreactive proteins were visualized using the ECL Select Western Blotting Detection System (GE Healthcare). Signals were detected on a Las-4000 mini instrument (GE Healthcare ...
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bioRxiv - Plant Biology 2021Quote: ... 50 μl of pre-washed protein-G sepharose-4 fast flow (GE Life Sciences) was added to each tube and tumbled end-over-end for 4 h at 4°C ...
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bioRxiv - Immunology 2020Quote: ... The protein was further purified on a Superose 6 gel filtration column (GE Healthcare) using an AKTA Pure FPLC instrument (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... The protein was first purified with a Ni2+-nitrilotriacetic acid (NTA) resin (GE Healthcare,) using an elution buffer consisting of 50 mM Tris-HCl ...
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bioRxiv - Microbiology 2021Quote: ... proteins were blotted onto a nitrocellulose membrane (Hybond-C Extra, GE Healthcare, Munich, Germany) Ponceau S staining of the blotted membrane was performed to assure equal amounts of proteins ...
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bioRxiv - Microbiology 2020Quote: ... proteins were transferred onto PVDF membranes (Amersham Hybond-LFP membranes [GE Healthcare, Buckinghamshire, UK]), or nitrocellulose membranes (Amersham Protran ...
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bioRxiv - Neuroscience 2020Quote: ... The eluted proteins were further purified via gel filtration (Superdex200, GE Healthcare Chicago, IL).
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bioRxiv - Biophysics 2020Quote: ... The labelled protein was further analysed by size-exclusion chromatography (Superdex 200, GE Healthcare) to enrich the double-labelled fraction and remove potential aggregation material131 ...
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bioRxiv - Microbiology 2021Quote: ... Protein bands were developed using the ECL™ Western Blotting Detection Reagent (GE Healthcare) and chemiluminescence was visualized using a ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Cell Biology 2020Quote: ... The protein was finally purified by Superdex 75 10/300 GL column (GE Healthcare) with buffer B.
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bioRxiv - Microbiology 2020Quote: ... The full-length ectodomain proteins were purified using HisTrap FF column (GE Life Sciences) in elution buffer containing 20 mM sodium phosphate ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoreactive protein was visualized using an enhanced chemiluminescence analysis kit (GE HealthCare, Piscataway, NJ).