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Citations for GE Life Sciences :
1751 - 1800 of 4331 citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Buffer replacement in protein purification used Column PD 10 desalting column (GE Healthcare). All protein purity was estimated as >95% by SDS-PAGE and protein concentration was measured spectrophotometrically (NanoVue ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were purified on a Ni-NTA HiTrap HP column (GE Life Sciences) and the His tag was removed by overnight cleavage with TEV protease (homemade) ...
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bioRxiv - Biophysics 2022Quote: ... Eluted protein was desalted using a PD10 column (GE Healthcare, Cat. no. 17085101) in 20 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... NQO1 proteins were purified using immobilized nickel affinity chromatography columns (IMAC, GE Healthcare) as described (12) ...
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bioRxiv - Biochemistry 2022Quote: ... Tagged proteins were purified by affinity chromatography (HisTrap resp. StrepTrap, both GE Healthcare) with elution buffer containing 200 mM imidazole respectively 2.5 mM d-desthiobiotin ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were further purified by SEC on a Superdex 75 (GE Healthcare) column ...
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bioRxiv - Immunology 2022Quote: ... Monoclonal antibodies were immobilized on Series S Protein G sensor chip (GE Healthcare) at a density of 2,000 response units (RU) ...
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bioRxiv - Microbiology 2022Quote: ... The dimeric ACE2 protein was purified by GammaBind Plus Sepharose beads (GE Healthcare), followed gel filtration chromatography on a Superdex 200 Increase 10/300 GL column ...
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bioRxiv - Microbiology 2022Quote: ... Materials and equipment for protein purification were obtained from GE Healthcare (Munich, Germany).
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bioRxiv - Molecular Biology 2022Quote: ... GST fusion proteins were immuno-precipitated using Glutathione Sepharose 4B beads (GE Healthcare). Fusion protein-bound beads were used directly in GST pull-down assays ...
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bioRxiv - Microbiology 2022Quote: ... protein was loaded onto a Superdex 200 Increase 10/300 column (GE Healthcare) with TBS buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins separated on gels were electroblotted onto nitrocellulose (Protran 0.2 μm, GE Healthcare) with a constant current of 100 mA for 10 h using wet transfer ...
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bioRxiv - Plant Biology 2023Quote: ... the antibodies were further affinity purified using Protein G mag sepharose (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... the supernatants were precleared by incubating with protein G-Sepharose beads (GE Healthcare), and reacted with 100 μL of Strep-Tactin Sepharose beads (IBA Lifesciences ...
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bioRxiv - Molecular Biology 2022Quote: ... on an ÄKTA explorer fast protein liquid chromatography (FPLC) system (GE Life Sciences) in 20 mM HEPES ...
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bioRxiv - Molecular Biology 2022Quote: ... overnight at 4°C in the presence of Protein A beads (GE Healthcare). ChIP-Seq libraries were prepared using the Kapa LTP Library Preparation Kit (Kapa Biosystems) ...
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bioRxiv - Molecular Biology 2022Quote: ... were applied along with Protein G Sepharose 4 Fast Flow beads (GE Healthcare). All ChIP extracts were pre-cleaned for 1 hr with 30 μl beads on a rotor at 4°C in absence of the antibody ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies were purified from hybridoma supernatant using HiTrap Protein G columns (GE Healthcare).
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bioRxiv - Immunology 2022Quote: ... The separated proteins were transferred on Amersham Protran nitrocellulose membrane (GE Healthcare Lifescience). After blocking with 5 % skimmed milk in TBS-T (0.1 % Tween-20 (Sigma-Aldrich ...
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: ... GST-RIG-I protein variants were affinity purified by GSTrap column (GE Healthcare). After sample loading ...
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bioRxiv - Microbiology 2024Quote: ... Protein bands reacting with antibodies were visualized using chemiluminescent detection reagent (GE Healthcare) on an iBright FL1500 (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2024Quote: ... the supernatants were combined with Protein G Sepharose beads (GE Healthcare, Illinois, USA) and incubated at 4 °C for an hour ...
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bioRxiv - Microbiology 2024Quote: ... The NRP2-Fc was purified using a Protein-A affinity column (GE Healthcare). NRP2-Fc point mutations were introduced by PCR mutagenesis using Kapa HiFi DNA polymerase (Kapa Biosystems) ...
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bioRxiv - Plant Biology 2023Quote: ... GST fusion proteins were purified on Glutathione-Sepharose® 4B beads (GE Healthcare) and eluted using a solution of 50LmM Tris-HCl pH 8 ...
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bioRxiv - Immunology 2024Quote: ... Lysate pre-cleaning steps were performed using Protein G Sepharose beads (GE Healthcare). To measure the protein content of the lysate a Qubit 4 fluorometer was used using the Qubit protein assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... supernatants were harvested and IgG was purified on Protein G Sepharose (GE Healthcare)
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bioRxiv - Biophysics 2024Quote: ... we loaded the protein solution onto a HiTrap Q FF column (GE Healthcare) and eluted it with a linear gradient of 0–1 M NaCl in 20 mM sodium phosphate at pH 6.0 ...
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bioRxiv - Biochemistry 2024Quote: ... eluted protein was loaded onto a HiPrep 26/10 desalting column (GE Healthcare) containing 25 mM Tris pH 7.5 at 20 °C ...
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bioRxiv - Biochemistry 2024Quote: ... and the protein was further purified by Q Sepharose column chromatography (GE Healthcare) and gel-filtration column chromatography (HiLoad Superdex75 16/60 ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were detected using a luminescence image analyzer (LAS-4000 mini; GE Healthcare) or a luminescence image analyzer (FUSION Solo S ...
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bioRxiv - Cancer Biology 2023Quote: ... Then proteins were transferred to a nitrocellulose membrane (Whatman, GE Healthcare Life Sciences). After transfer ...
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bioRxiv - Biophysics 2024Quote: ... the protein was passed through a 5 mL HisTrap HP column (GE Healthcare), and the cleaved protein was collected in the flow through ...
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bioRxiv - Immunology 2022Quote: ... Protein bands were visualized using ECL Prime Western Blotting Detection Reagent (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... The protein bands were detected using ECL (RPN2232, GE Healthcare, Little Chalfont, UK) on Amersham Imager 600 ...
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bioRxiv - Microbiology 2023Quote: ... The eluted protein was loaded on a HiTrap Q HP column (GE Healthcare) in buffer consisting of 20 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were then transferred to a nitrocellulose membrane (Whatman, GE Healthcare Life Sciences), which was blocked with 5% dry-milk-Tris Buffer saline ...
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bioRxiv - Biochemistry 2023Quote: ... The cleaved protein was then reapplied to a GSTrap HP column (GE Healthcare), and the flow-through fraction was collected ...
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bioRxiv - Microbiology 2023Quote: ... Each protein was purified on a Ni Sepharose High Performance column (GE Healthcare). The purified fractions were desalted and concentrated using an Amicon Ultra centrifugal filter unit (10-kDa cutoff ...
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bioRxiv - Cell Biology 2023Quote: GST-tagged proteins were affinity-purified using glutathione-Sepharose 4B beads (GE Healthcare) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were further purified using anion-exchange chromatography (Source 15Q, GE Healthcare) using a salt gradient from 0 to 1 M NaCl in a buffer containing 20 mM TrisHCl ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were pre-cleared with protein A-Agarose beads (GE Healthcare, Chicago, USA) for 1 h in a wheel shaker at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... the proteins were transferred onto a nitrocellulose membrane (Hybond-C Extra, GE Healthcare) and an anti-His-HRP conjugate antibody (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... the proteins and domains were purified by ÄKTA Go System (GE Healthcare, USA) with Ni-NTA affinity chromatography column (HisTrap HP ...
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pADP-ribosylation Regulates the Cytoplasmic Localization, Cleavage and Pro-apoptotic Function of HuRbioRxiv - Cell Biology 2023Quote: ... were incubated with 60 µl of protein A-Sepharose slurry beads (GE Healthcare) (washed and equilibrated in cell lysis buffer ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... mRNA was purified by Fast Protein Liquid Chromatography (FPLC) (Akta Purifier, GE Healthcare) [21] ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were wet transferred to nitrocellulose membrane (0.45 μM, GE Healthcare Life Sciences) at 100 V for 75 min ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was purified with Superose 6 Increase 10/300 column (GE Healthcare) (Fig ...
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bioRxiv - Plant Biology 2023Quote: ... Tagged proteins were purified separately using glutathione agarose beads (GE Healthcare, Chicago, USA) or Ni-NTA affinity agarose beads (QIAGEN) ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was further purified on a Mono S cation exchange column (GE Healthcare) and stored in CHIP TPR storage buffer (10 mM Tris ...
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bioRxiv - Biophysics 2023Quote: ... A series of chromatography in a fast protein liquid chromatography system (GE Healthcare) was performed [31] ...