Labshake search
Citations for GE Life Sciences :
1901 - 1950 of 2093 citations for Anti Syndecan 4 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2020Quote: ... Anti-NPR1 antibodies were purified from antisera by affinity chromatography using a tNPR1-His-immobilized column (Hitrap NHS-activated HP; GE Healthcare). To prepare for the tNPR1-His-immobilized column ...
-
bioRxiv - Systems Biology 2020Quote: ... the target-specific antibody was added to the cellular lysates together with 25 μl of Protein A or G sepharose (GE Healthcare) depending on the species of target antibody and the mixture was incubated overnight rotating at 4°C ...
-
bioRxiv - Microbiology 2021Quote: ... Background binding to reference flow cells was subtracted and antibody binding levels were calculated using Biacore T200 evaluation software (GE Healthcare).
-
bioRxiv - Molecular Biology 2020Quote: ... AlexaFluor conjugated secondary antibodies targeting respective primary antibodies (Thermoscientific, USA) were used to visualize red (IdU) and green (CldU) DNA fibers on a Deltavision microscope (Applied Precision) and analyzed using softWoRx 5.5 software ...
-
bioRxiv - Cancer Biology 2020Quote: ... Membranes were incubated with HRP-coupled secondary antibodies (santa cruz, cell signaling) for 1 h at RT and chemiluminescence detected with ECL Prime (GE Healthcare) at the LAS-1000 (Fujifilm) ...
-
bioRxiv - Immunology 2022Quote: The binding kinetics and affinity of recombinant monoclonal antibodies for the SARS-CoV-2 RBD protein (SinoBiological) were analyzed by SPR (Biacore T200, GE Healthcare). Specifically ...
-
bioRxiv - Microbiology 2022Quote: Kinetic interactions between the antibodies and his-tagged antigen proteins were measured at room temperature using Biacore T200 surface plasmon resonance (GE Healthcare). Anti-human fragment crystallizable region (Fc region ...
-
bioRxiv - Biochemistry 2022Quote: ... Membranes were then washed with TBS-T (3x, 5 min each) and incubated with horse radish peroxidase (HRP)-conjugated secondary antibodies (GE Healthcare). Following another wash step in TBS-T ...
-
bioRxiv - Biochemistry 2022Quote: Antibody binding to SARS-CoV-2 spike was assessed using SPR on a Biacore T-200 (Cytiva, MA, formerly GE Healthcare) with HBS buffer supplemented with 3 mM EDTA and 0.05% surfactant P-20 (HBS-EP+ ...
-
bioRxiv - Immunology 2020Quote: ... The bound antibodies were eluted and further purified by gel-filtration chromatography using a Superdex 200 High Performance column (GE Healthcare). The purified antibodies were either used in binding and neutralizing assays.
-
bioRxiv - Immunology 2020Quote: ... Recombinant IgG antibodies were purified from cell-free supernatants by affinity purification using Protein G or Protein A sepharose beads (GE Healthcare). Purified proteins were dialyzed in PBS ...
-
bioRxiv - Cell Biology 2021Quote: ... Horseradish peroxidase-conjugated secondary antibodies and enhanced chemiluminescence solution (MoBiTec) were used for visualization on an Amersham Imager 680 (GE Healthcare) or a Cawomat 2000 IR apparatus (CAWO solutions).
-
bioRxiv - Cell Biology 2021Quote: ... followed by incubation with horseradish peroxidase-conjugated secondary antibodies (Table S6) and detected with enhanced chemiluminescence reagents (Amersham Bioscience) using Amersham hyperfilm (GE Healthcare) or the G:Box Chemi (Syngene) ...
-
bioRxiv - Biochemistry 2021Quote: The binding affinities of full IgG antibodies to SARS-CoV-2 spike protein were determined using surface plasmon resonance (SPR) and a BIAcore T200 instrument (GE Healthcare) at 25°C ...
-
bioRxiv - Immunology 2022Quote: ... The antibody and RBD affinity ability were estimated employing a 1:1 binding fit model in Biacore Evaluation software (GE Healthcare).
-
bioRxiv - Immunology 2019Quote: ... Membranes were blocked with 5% bovine serum albumin and incubated with the appropriate primary and HRP-conjugated secondary antibodies (GE Healthcare). Western Lightning ECL Pro (PerkinElmer ...
-
bioRxiv - Cell Biology 2020Quote: ... Membranes were probed using the indicated antibodies and chemiluminescent signals was detected by a LAS 4000 mini Image Quant system (GE Healthcare). Band intensity was quantified using ImageJ ...
-
bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed three times 10 minutes and incubated for at least one hour in HRP-conjugated secondary antibodies (GE Healthcare) at 1:8000 dilution in PBS-T ...
-
bioRxiv - Microbiology 2020Quote: ... blocked for 1 hour and immuno-detected using Anti-RecA (E. coli) antibody (1000x dilution) (gift from Mike Cox, (52)) and analyzed using an ECL Prime detection kit (GE Healthcare). Densitometry was performed using ImageJ (https://imagej.nih.gov/ij/)
-
bioRxiv - Neuroscience 2020Quote: ... Immunoprecipitation was performed with the indicated primary antibodies and 50 μl of a 50% slurry of protein-A sepharose beads (GE Healthcare) for 2 h at 4°C ...
-
bioRxiv - Physiology 2020Quote: ... Blots were then washed in TBST and incubated for 1 h at 22-24°C with secondary HRP-conjugated antibodies (GE Healthcare) diluted 5000-fold in 5% skim milk in TBST ...
-
bioRxiv - Cell Biology 2019Quote: ... the antibodies were separated from unconjugated dye by gravity filtration through Sephadex G-25 DNA grade size exclusion columns (GE Healthcare) by visual detection ...
-
bioRxiv - Cell Biology 2020Quote: ... antigen was CENP-AHCP-3(105-183)::6xHis) and detected using an HRP-conjugated secondary antibody (rabbit or mouse; GE Healthcare). For antibody production CENP-AHCP-3(105-183)::6xHis was expressed in E ...
-
bioRxiv - Immunology 2021Quote: ... All antibody formats were expressed using ExpiCHOTM Expression system (Thermoscientific, USA) and purified on HiTrap Protein-A column (GE healthcare, UK). The integrity and purity of the antibodies were analyzed using SDS-PAGE.
-
bioRxiv - Immunology 2021Quote: The binding affinities of antibodies to SARS-CoV-2 spike protein were determined using surface plasmon resonance (SPR) and a BIAcore T200 instrument (GE Healthcare) at 25°C ...
-
bioRxiv - Immunology 2020Quote: ... the wells were washed with PBS containing 0.05 % Tween®20 (PBS-T) and incubated with HRP-conjugated antibodies (GE healthcare) for 3 hours at RT ...
-
bioRxiv - Genetics 2020Quote: 200 µg of antibodies R1 and R2 (against HSF2BP and MIDAP) were crosslinked to 100 ul of sepharose beads slurry (GE Healthcare). Testis extracts were prepared in 50mM Tris-HCl (pH8) ...
-
bioRxiv - Microbiology 2022Quote: ... Antibody supernatant was harvested 6 days following transfection and purified using protein G affinity chromatography following the manufacturers protocol (GE Healthcare). Specificity of the cloned mAb was determined by ELISA.
-
bioRxiv - Developmental Biology 2022Quote: ... Secondary antibodies coupled to horseradish peroxidase were used for enhanced chemiluminescence signal detection with ECL Prime Western Blotting System (GE Healthcare).
-
bioRxiv - Cancer Biology 2022Quote: ... were resuspended in 500 μl IP buffer and incubated with the appropriate antibodies on ice for 2 h and then 30 μl protein-G-sepharose beads (GE Healthcare; equilibrated once in 10 ml water and three times in washing buffer ...
-
bioRxiv - Microbiology 2022Quote: ... and bands visualized with (HRP)- coupled secondary antibody (1:5,000) (Cell Signalling, rabbit 7074S, mouse 7076S) on an ImageQuant LAS 4000 Mini (GE Life Sciences) with Luminata Forte Western HRP Substrate (Sigma WBLUF0500 ...
-
bioRxiv - Immunology 2022Quote: ... RPAPGST(GalNAc)APPAHG and RPAPGS(GalNAc)T(GalNAc)APPAHG with immobilized antibody 14A and 16A were determined by surface plasmon resonance on a Biacore T200 (GE Healthcare) instrument ...
-
bioRxiv - Immunology 2022Quote: ... Membranes were then incubated with the corresponding HRP-tagged secondary antibodies in BSA (5% w/v) PBS-T for 1 hour before visualisation using Amersham ECL prime detection reagent (GE healthcare) and a G:Box Chemi XX6 system (Syngene) ...
-
bioRxiv - Biophysics 2022Quote: ... 3 volumes of 1 mg/mL affinity-purified antibody was gently rotated with 1 volume Protein A Sepharose beads (GE Healthcare) overnight at 4°C or 1 hour at room temperature ...
-
bioRxiv - Immunology 2022Quote: ... The ACE2 receptor or SARS-CoV-2 spike-specific antibodies (CR3022 or S309) were immobilized on the protein A sensor chip (GE Healthcare) at a ligand capture level of ∼100 RU ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteins were visualized by incubation with the indicated antibodies and developed using the chemiluminescence function on an Amersham Imager 600 (GE Healthcare) or a Compact 2 developer (Protec) ...
-
bioRxiv - Cell Biology 2023Quote: ... Blots were incubated with indicated primary and HRP-conjugated secondary antibodies then detected using Amersham ECL Western blotting reagents (GE Healthcare) or for more sensitive detection SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Scientific ...
-
bioRxiv - Developmental Biology 2022Quote: ... was used as the secondary antibody. Protein was detected using the chemiluminescent substrate ECL (Cat. # 32209, Pierce) in an Amersham Imager 680 (GE Healthcare) detector.
-
bioRxiv - Developmental Biology 2023Quote: ... Then it was incubated with a secondary antibody and detected using a Medical Film Processor (SRX-101A) or ImageQuant LAS 4000 (GE Healthcare).
-
bioRxiv - Neuroscience 2023Quote: ... Blots were washed three times in PBST for 15 min and were then incubated with HRP-conjugated secondary antibodies (1:2,500 dilution; GE Healthcare, NA9340V) for 45 min at room temperature ...
-
bioRxiv - Immunology 2023Quote: The binding kinetics and affinity of monoclonal antibodies to the SARS-CoV-2 spike trimer were analyzed by SPR (Biacore T100, GE Healthcare). Specifically ...
-
bioRxiv - Cancer Biology 2023Quote: ... Membranes were subsequently washed with TBST (0.1% Tween-20) and incubated with an HRP-conjugated secondary antibody (GE Healthcare Bio-Sciences). After washing with TBST ...
-
bioRxiv - Microbiology 2023Quote: ... Membranes were washed 3x for 10 min with TBST and subsequently incubated for 1 hr at room temperature with appropriate horseradish peroxidase-conjugated secondary antibodies (GE Healthcare) diluted (1:10,000 ...
-
bioRxiv - Biophysics 2020Quote: ... The supernatant was loaded into gravity column containing anti-FLAG M2 affinity gel (GE Healthcare). The resin was washed with wash buffer (25 mM HEPES pH 7.4 ...
-
bioRxiv - Plant Biology 2020Quote: ... The bound HY5 was detected by anti-GST-HRP conjugate (RPN1236; GE Healthcare Bio-Sciences). Membranes were developed and visualized as described above ...
-
bioRxiv - Systems Biology 2020Quote: ... washed in TBST and detected with Sheep anti-Mouse IgG-Horseradish peroxidase (HRP; GE Healthcare Life Science ...
-
bioRxiv - Biochemistry 2020Quote: ... and HRP-linked anti-mouse (GE Healthcare, #NA931-1ML, at a dilution of 1:5000) as the secondary antibody ...
-
bioRxiv - Microbiology 2020Quote: ... mAb followed by horseradish peroxidase (HRP)-linked anti-mouse IgG (1/2,000, NXA931, GE Healthcare). Periplasmic fractions ...
-
bioRxiv - Cell Biology 2022Quote: ... HRP-conjugated anti-mouse IgG (Jackson ImmunoResearch Laboratories, 315-035-003 or GE Healthcare, #NA931).
-
bioRxiv - Immunology 2022Quote: ... followed by staining with the horseradish peroxidase (HRP)-conjugated anti-rabbit (1:50,000; GE healthcare) and anti-mouse (1:25,000 ...