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Citations for GE Life Sciences :
1601 - 1650 of 2093 citations for Anti Syndecan 4 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... donkey anti-rabbit (HRP-conjugated, GE Healthcare, NA934V, 1:10000).
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bioRxiv - Microbiology 2023Quote: ... with an anti-rabbit IgG (1:10,000; GE Healthcare, #RPN4301) secondary antibody was used for normalization after FLAG/GFP detection ...
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bioRxiv - Immunology 2023Quote: ... anti-mouse IgG-HRP (NA931V; 1/1000 dilution, GE Healthcare), anti-rabbit IgG-HRP (NA934V ...
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bioRxiv - Immunology 2024Quote: ... such as peroxidase-conjugated anti-mouse IgG (#NA931; GE Healthcare) or peroxidase-conjugated anti-rabbit IgG (#NA934 ...
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bioRxiv - Neuroscience 2023Quote: ... or anti-mouse (GE Healthcare UK Limited NA931V, lot #: 9814763) secondary antibodies (1:10000 ...
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bioRxiv - Cell Biology 2024Quote: ... anti-rabbit (HRP conjugated donkey, 1:2000, GE Healthcare, #NA934); anti-mouse (HRP conjugated goat ...
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bioRxiv - Cell Biology 2024Quote: ... anti-GFP nanobody covalently coupled to NHS-agarose (GE Healthcare) and guinea pig anti-Rad21 and guinea pig anti-Pds5b coupled to protein A sepharose (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was centrifuged at 9,400× g at 4 °C for 20 min and the supernatant was applied onto a 5 mL His-Trap TALON crude column (GE Healthcare Bioscience, Piscataway, NJ, USA) pre-equilibrated with deionized water and Talon binding buffer ...
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bioRxiv - Bioengineering 2019Quote: ... cells debris were removed by centrifugation at 16,000 × g for 20 min at 4 °C and the supernatant (supplemented with 20 mM Imidazole) was loaded on a 1 mL Ni+-HisTrapHP column (GE Healthcare #71-5027-68 AK) and washed with a buffer containing 100 mM Tris-HCl (pH 7) ...
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bioRxiv - Cell Biology 2020Quote: ... This protein was coupled to a 1 ml packed column volume of NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare Life Sciences, Marlborough, MA). 10 ml of serum was diluted 1:1 in binding buffer (20mM Tris ...
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bioRxiv - Pathology 2021Quote: ... 250 μg of total protein was loaded on a 13 cm Immobiline dry-strip (pH 4-7, M/s. GE Healthcare Life Sciences, Amersham, UK), on a rehydration tray ...
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bioRxiv - Biochemistry 2021Quote: ... Secreted protein was captured by two round of batch absorption (BA) with 4 mL/L of pre-equilibrated Ni Sepharose beads (GE Healthcare, Cat #17-5318-01). The bound beads were transferred to gravity flow column and sequentially washed with 30 CVs Wash Buffer (50 mM HEPES 7.5 ...
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bioRxiv - Plant Biology 2022Quote: ... chromosomal DNA was purified and amplified to 5.4 - 7.9 μg by multiple displacement amplification (Supplementary Table 3) using an Illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare, Chalfont St. Giles, United Kingdom) as described by Šimková et al ...
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bioRxiv - Immunology 2023Quote: ... Eluate was then cleaved with TEV protease overnight at 4 °C and subsequently purified further by size exclusion chromatography (Superdex 200 26/600 from GE Healthcare; cat # GE28-9893-36) in TBS ...
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bioRxiv - Biochemistry 2023Quote: ... 55 µg of protein from pulse-labeled samples for analytical gels and 300 µg of protein from unlabeled samples for preparative gels were rehydrated into IPG strips (24 cm, pH 4-7; GE Healthcare, Chalfont St Giles, UK). A Multiphor II (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... pombe lysate were mixed and incubated at 4 °C for 30 min before the addition of glutathione-sepharose beads (GE Healthcare, Cat#17-0756-01). After 2-hour incubation ...
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bioRxiv - Immunology 2021Quote: Purification of the recombinant antibodies was conducted on a ÄKTAprime plus system (GE Healthcare Life Sciences) at 4°C using a 1 mL HiTrap® Protein A HP pre-packed Protein A Sepharose® column (Cytiva) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the bound antibodies were visualized by chemiluminescence using the ECL plus reagent (GE Healthcare, Buckinghamshire, UK). The GAPDH content was analyzed as the loading control using a rabbit polyclonal antibody.
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies were detected using mouse IgG HRP linked whole Ab (1:4000; NXA931; GE healthcare).
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bioRxiv - Cancer Biology 2019Quote: ... or a combination of the appropriate antibody and Protein A or G sepharose beads (GE Healthcare) were used to immunoprecipitate tagged or endogenous proteins from cleared lysates ...
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bioRxiv - Neuroscience 2020Quote: ... the membrane was incubated with Horseradish Peroxidase (HRP)-conjugated secondary antibody (GE healthcare, NXA931V or NA934V) in 1% non-fat BSA in PBST at room temperature for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... 0.1mg of purified antibody was injected into the AKTA protein purification system (GE Healthcare Life Sciences) and protein fractions were separated using a Superdex 200 10/300 column (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2020Quote: ... Secondary horseradish peroxidase (HRP)-conjugated antibody dilutions were used as follows: goat α-rat (GE Healthcare) 1:2,000 ...
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bioRxiv - Systems Biology 2020Quote: ... The following secondary antibodies were used for immunoblotting: ECL rabbit HRP-linked IgG (GE Healthcare, NA934) and ECL mouse HRP-linked IgG (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... Incubation using the secondary antibody α-mouse IgG (H&L)-HRP (1:10,000) (GE healthcare #NXA931) diluted in blocking buffer was performed for two hours ...
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bioRxiv - Biochemistry 2022Quote: ... the membrane was incubated with HRP conjugated secondary antibody (IgG; mouse or rabbit) (GE Healthcare UK) at 1:5000 dilution in blocking buffer and incubated at RT with shaking for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies were washed and immunoreactive bands were developed for five minutes with ECF (GE Healthcare) and revealed in a Chemidoc Imaging System (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: ... either a 1:10,000 dilution of α-mouse-HRP conjugated antibody (GE Healthcare Life Sciences NA931) or a 1:2,000 dilution of α-mouse-HRP conjugated antibody (used for western blot of immunoprecipitations ...
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bioRxiv - Cell Biology 2022Quote: ... antibody-antigen complexes were detected by FUSION SOLO S (VILBER) using an ECL system (GE Healthcare). Quantification was performed via densitometry using Fusion Capt software (VILBER) ...
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bioRxiv - Molecular Biology 2020Quote: ... and then incubated for 1 h with ECL mouse IgG HRP-conjugated whole antibody (GE Healthcare) or rabbit IgG HRP-conjugated whole antibody (GE Healthcare) ...
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bioRxiv - Immunology 2020Quote: ... the antibodies secreted into the supernatant were collected and captured by protein A Sepharose (GE Healthcare). The bound antibodies were eluted and further purified by gel-filtration chromatography using a Superdex 200 High Performance column (GE Healthcare) ...
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bioRxiv - Neuroscience 2022Quote: ... The binding of HRP-conjugated secondary antibodies was revealed by ECL Plus (GE Healthcare Life Sciences) and chemiluminescence was measured using a ChemiDoc XRS apparatus (BioRad) ...
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bioRxiv - Immunology 2022Quote: ... the supernatants consisting of secreted antibodies were collected and trapped by protein G Sepharose (GE Healthcare). The bound antibodies on the Sepharose were eluted and concentrated using Centricon Plus-70 (051555 ...
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bioRxiv - Microbiology 2022Quote: ... The supernatants were filtered and purified by HiTrap Protein G HP antibody purification columns (GE Healthcare).
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bioRxiv - Genetics 2019Quote: ... and the secondary antibodies were visualized by ECL Prime Western Blotting Detection Reagents (GE Healthcare, UK).
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed three times with TBST before incubation with secondary HRP-conjugated antibodies (GE Healthcare). Membranes were further washed three times with TBST before ECL exposure.
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bioRxiv - Immunology 2020Quote: ... The secreted antibodies were subsequently purified from the supernatant on HiTrap Protein G columns (GE Healthcare). Plasmid DNA encoding a gliadin-reactive IGHV4-4:IGKV4-1 antibody with R in position 55 (corresponding to the IGHV4-4*07 allele ...
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bioRxiv - Immunology 2019Quote: ... Antibody binding was visualized with Amersham ECL Western blotting detection reagent (GE Healthcare, Little Chalfont, UK).
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bioRxiv - Cell Biology 2019Quote: ... Signals were detected with HRP-conjugated secondary antibodies and visualized with ECL detection reagent (GE Healthcare).
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bioRxiv - Genetics 2020Quote: ... membrane was incubated with horseradish peroxidase-conjugated secondary antibodies before analyses by enhanced chemiluminescence (GE Healthcare). B-Actin was used as loading control.
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bioRxiv - Neuroscience 2021Quote: ... followed by 1 h at room temperature in the appropriate HRP-labelled secondary antibody (GE Healthcare). Blots were developed with ECL Clarity (GE Healthcare).
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was concentrated and purified over HiTrap Protein G HP antibody purification column (GE Healthcare). To visualize 410C9 in vivo ...
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bioRxiv - Immunology 2020Quote: ... The washed membrane was incubated with a secondary antibody labeled with horseradish peroxidase (HRP) (GE healthcare) for 1 hour at RT ...
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bioRxiv - Developmental Biology 2022Quote: ... Horseradish peroxidase (HRP)-conjugated secondary antibodies and the ECL prime western blotting system (GE Healthcare, RPN2232) were used for detection of primary antibodies ...
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bioRxiv - Biochemistry 2022Quote: Antibodies against CFTR were pre-incubated with protein-A or protein-G Sepharose beads (GE Healthcare) for 15 minutes at 4°C before adding protease-treated or non-treated lysates ...
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bioRxiv - Genetics 2023Quote: ... Secondary antibodies: 1:10000 goat α rabbit or goat α mouse ECL (GE healthcare NA931, NA934).
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bioRxiv - Immunology 2023Quote: ... The antibodies were purified by affinity chromatography using a Protein A resin (MabSelect SuRe, GE Healthcare), followed by a preparative size exclusion chromatography (SEC) ...
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bioRxiv - Developmental Biology 2023Quote: ... Horseradish peroxidase (HRP)-conjugated secondary antibodies and the ECL prime western blotting system (GE Healthcare, RPN2232) were used for detection of primary antibodies ...
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bioRxiv - Immunology 2023Quote: Monavalent SPR of CD19 antibodies was conducted on BI-Acore T200 instrument (GE Healthcare Life Sciences). The experiment was run at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... The antibody binding was visualized using Amersham ECL Prime Western Blotting Detection Reagent (GE healthcare; #RPN2236) and imaged with the ImageQuant LAS400 Imaging unit (GE Healthcare).