Labshake search
Citations for GE Life Sciences :
1801 - 1850 of 2632 citations for Mouse Eukaryotic Translation Termination Factor 1 ETF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... and then with anti-Rabbit IgG Horseradish Peroxidase linked whole antibody (1:5000; GE Healthcare; NA943V). Membrane was visualized using Radiance Plus chemiluminescent HRP substrate (Azure Biosystems ...
-
bioRxiv - Biochemistry 2022Quote: ... and then with anti-Rabbit IgG Horseradish Peroxidase linked whole antibody (1:5000; GE Healthcare; NA943V). Membrane was visualized as described above.
-
bioRxiv - Cell Biology 2022Quote: ... and cloned into BamHI and NotI restriction sites within the pGEX-4T-1 plasmid (GE Healthcare), such that the gene was in frame with an N-terminal GST tag (pGEX-4T-1-GST-KIN-EC-ter) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Recombinant proteins were then purified by 1 mL Ni Sepharose (17-5318-01, GE Healthcare, USA). Columns were sequentially washed with 20 mL buffer 1 and 10 mL buffer 2 (in mM ...
-
bioRxiv - Biochemistry 2023Quote: ... The diluted sample was then applied to a 1 mL HiTrap Q HP column (GE Healthcare), and UBXD1 was eluted with a 0-50% gradient of AEX elution buffer (AEX binding buffer supplemented with 1000 mM KCl) ...
-
bioRxiv - Plant Biology 2023Quote: ... The supernatant was filtered (Filter paper, Grade 1; Size: 185 mm; Whatman, GE Healthcare Live Sciences), a 1 mL aliquot of the filtrate was transferred into a 1.5 mL centrifuge tube ...
-
bioRxiv - Biochemistry 2023Quote: ... the solubilized enzymes were loaded onto a 1 mL Q-sepharose HP column (GE Healthcare, UK) pre-equilibrated in buffer containing 20[mM Tris-HCl (pH 7.14 at 20[°C) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Amplified cpxA31-163 was cloned into BamHI/EcoRI-digested pGEX-6P-1 expression vector (GE Healthcare), which encodes for an N-terminal glutathione S-transferase (GST ...
-
bioRxiv - Cancer Biology 2023Quote: ... membranes were probed with an ECL anti-rabbit IgG secondary antibody (1:10,000) from GE Healthcare Life Science and imaged using Chemidoc Molecular Imaging System (BioRad) ...
-
bioRxiv - Biochemistry 2023Quote: ... The supernatant was loaded on a 1 ml HisTrap HP Q column (GE Healthcare, Pittsburgh, PA), and the flow-through was collected and dialyzed against 20 mM HEPES pH 6.8 ...
-
bioRxiv - Cell Biology 2023Quote: ... Biotin incorporation was assessed by incubating blots with HRP-streptavidin (1:1000 v/v, GE Healthcare) and vimentin levels were estimated by western blot as described below ...
-
bioRxiv - Biophysics 2023Quote: ... 1 mM DTT) and applied to a Superdex 200 10/300 size exclusion column (GE Healthcare) that was pre-equilibrated with cross-linking buffer (20 mM HEPES pH 7.5 ...
-
bioRxiv - Molecular Biology 2024Quote: ... The supernatant was applied to 1 ml Nickel resin (Ni Sepharose 6 Fast Flow, GE Healthcare) preequilibrated with 20 ml buffer A and 20 mM imidazole ...
-
bioRxiv - Biochemistry 2024Quote: ... 1 mM DTT) was exchanged to a storage buffer omitting DTT using a PD10 (GE Healthcare) gel filtration column ...
-
bioRxiv - Biochemistry 2024Quote: ... 1 mM DTT) and purified with GST-BIN1_SH3 bound to Glutathione-Sepharose 4B beads (GE Healthcare). Human SH3 of BIN1 with GST tag (GST-SH3 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Chemiluminescence detection was performed using the ECL Prime Western Blotting kit (GE-healthcare, RPN2232) and developed on a ImageQuant LAS 4000 (GE healthcare, 28-9558-10). Secondary antibodies for infrared fluorescence detection were donkey anti-rabbit-IRDye® 800CW (Rockland ...
-
bioRxiv - Genomics 2020Quote: ... 2 μL of each field sample was subject to whole genome amplification using the illustra Genomiphi V2 DNA amplification kit (GE Healthcare Life Sciences, Australia) for 2 hours at 30°C followed by heat inactivation at 65°C for 10 minutes ...
-
bioRxiv - Molecular Biology 2020Quote: ... Blots were developed using the WesternBright™ Quantum kit (K-12042-D10, Avansta) and visualized using an Amersham Imager 680 (GE Healthcare Life Sciences) system.
-
bioRxiv - Cancer Biology 2019Quote: ... Staining was detected using an HRP-conjugated anti-rabbit secondary antibody and Chemi-Lumi One L assay kit (Nacalai) on a ImageQuant LAS 4000 imager (GE Healthcare, Chicago, IL, USA).
-
bioRxiv - Microbiology 2021Quote: ... PCR elongation was performed at 72 °C (2 min per kb) and the amplified products were purified by illustra GFX purification kit (GE Healthcare Little Chalfont, UK).
-
bioRxiv - Microbiology 2020Quote: ... the cDNA obtained in the section “Realtime and Endpoint RT-PCR detection of deltaviruses from animal specimens” was amplified using illustra GenomiPhi V2 Kit (GE healthcare; Chicago, IL, USA). The amplified DNA was then purified with innuPREP PCRpure Kit (Analytik Jena ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... on each FTA punch or on 2 µL or 4 µL of DNA solution using the Illustra GenomiPhi V2 DNA Amplification kit (GE Healthcare Life TM Sciences). Amplified DNA was purified with the SigmaSpin Sequencing reaction Clean-up (Sigma-Aldrich) ...
-
bioRxiv - Physiology 2021Quote: ... Membranes were again washed with 4 x 15 min TBS-T washes and a final 15 min TBS wash prior to band development with an ECL Prime Western Blot Detection Kit (GE Healthcare, Chicago, IL, USA) and imaged using a Chemidoc Imaging system (Bio-Rad ...
-
bioRxiv - Immunology 2021Quote: ... Recombinant RBD protein at a concentration 20 μg/ml in 10 mM acetate buffer (pH 4.5) was immobilized on the surface of a CM5 sensor chip using a Amine Coupling Kit (GE Healthcare Bio-Sciences AB, Sweden). VHHs were serial 4-fold diluted from 417 nM to 0 nM and flowed over the captured RBD surface at 15 μl/min for 3 minutes ...
-
bioRxiv - Microbiology 2022Quote: ... The samples were transferred to a positively charged membrane using southern blot protocol from the Whatman Nytran SuPerCharge TurboBlotter kit (Whatman GE Healthcare, Little Chalfont, UK). Air dry the membrane ...
-
bioRxiv - Microbiology 2022Quote: ... Multiple displacement amplification of genomic DNA was performed using the illustra Ready-To-Go GenomiPhi V3 DNA Amplification Kit (GE Healthcare, Piscataway, NJ, USA). Paired-end library was constructed using NEXTFLEX Rapid DNA-Seq (Bioo Scientific ...
-
bioRxiv - Microbiology 2023Quote: ... The remainder of the extraction was performed with a modified version of the Illustra RNAspin mini RNA isolation kit (GE Healthcare, #25-0500-71) protocol ...
-
bioRxiv - Cell Biology 2024Quote: ... PCR samples were resolved on agarose gel and amplified fragments extracted from gel using GFX PCR DNA and Gel Band purification kit (GE Healthcare, 28-9034-70). The purified DNA or 1µg of plasmid DNA were digested with XmaI and XbaI for 1 h at 37°C in 1x CutSmart buffer and nuclease-free water ...
-
bioRxiv - Plant Biology 2020Quote: ... The PuACO1 or PuERF2 CDSs were inserted into the pGEX4T-1 vector (GE Healthcare, http://www3.gehealthcare.com) downstream from GST ...
-
bioRxiv - Developmental Biology 2020Quote: ... and KDM2B1–734 were cloned into pGEX-4T-1 (GST tag vector) (GE Healthcare, Marlborough, MA, USA). The BL21 strain of E ...
-
bioRxiv - Molecular Biology 2020Quote: ... membranes were incubated with a 1:5000 dilution of anti-rabbit (GE Healthcare, Little Chalfont, UK, NA934V) or anti-mouse secondary antibody (GE Healthcare ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1 mM β-mercaptoethanol) and further purified by gel filtration over a Superdex-200 column (GE Healthcare). The protein product was concentrated with a centrifugal filter unit (MD Millipore ...
-
bioRxiv - Molecular Biology 2021Quote: ... chromatin was precleared for 1 hour at 4 °C with blocked protein A Sepharose beads (GE Healthcare, Eindhoven ...
-
bioRxiv - Biophysics 2020Quote: ... the sample supernatant fraction was incubated with 1 ml Glutathione Sepharose 4B (GE Healthcare, 17-0756-01) for 3 h at 4 °C to bind GST-tagged proteins ...
-
bioRxiv - Bioengineering 2019Quote: ... Whatman Grade 1 chromatography paper for the paper-based reactions was obtained from GE Healthcare (Mumbai, India). DNA ladder (O’GeneRuler ultra low range 10–300 bp ladder) ...
-
bioRxiv - Molecular Biology 2020Quote: ... 12.5% acrylamide:bis-acrylamide 19:1) electrophoresis and heat/vacuum dried for autoradiography (Amersham Hyperfilm ECL, GE Healthcare). Images were analyzed using ImageJ ...
-
bioRxiv - Biophysics 2019Quote: ... 4 mM β-mercaptoethanol and loaded onto a 1 ml MonoQ anion exchange chromatography column (GE Healthcare). After washing with 10 column volumes of buffer ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... HRP conjugated secondary antibodies were used at 1:10,000 and visualised using ECL (GE Healthcare, Buckinghamshire, UK), high performance chemiluminescence film (Amersham Hyperfilm ECL ...
-
bioRxiv - Biochemistry 2020Quote: ... The dialysed protein was loaded onto two His-Trap HP 1 mL columns (GE Healthcare, Chicago, IL) mounted in series and equilibrated with Buffer B ...
-
bioRxiv - Biochemistry 2020Quote: ... 5 mM MgCl2 and 1 mM CaCl2 and applied to a 5 mL HisTrap Excel (GE Healthcare). Afterwards the column was washed with 40 mL of 20 mM TRIS pH 8 ...
-
bioRxiv - Microbiology 2021Quote: ... 1% saline-sodium citrate [SSC]) at 50 °C and exposed to a phosphor screen (GE Healthcare, USA) overnight ...
-
bioRxiv - Biochemistry 2021Quote: Calorimetric titration of Mint3NT with FIH-1 was performed using a MicroCal™ iTC200 system (GE Healthcare) at 25°C ...
-
bioRxiv - Biochemistry 2021Quote: ... The N-terminal His6-tag was then removed by digestion with thrombin (≈ 1 U/mg; GE Healthcare) at 4°C overnight ...
-
bioRxiv - Biochemistry 2020Quote: ... Japan) was subcloned into the BamHI and XhoI sites of pGEX-4T-1 vector (GE Healthcare, Inc.) using standard PCR-based protocols ...
-
bioRxiv - Biochemistry 2020Quote: ... to < 1 mL and injected onto a SuperdexTM 75 10/300 GL size exclusion column (GE Healthcare) equilibrated in Gel Filtration buffer (20 mM HEPES pH 7.7 ...
-
bioRxiv - Microbiology 2020Quote: ... The eluted protein was desalted against 1 x PBS buffer and digested with thrombin protease (GE Healthcare) in a ratio of 1 unit per μg at room temperature ...
-
bioRxiv - Microbiology 2021Quote: ... Eranthie Weerapana) for 1 h at 22 °C and desalted (PD Miditrap G-25 columns, GE Healthcare). Each light lysate was then combined 1:1 with a heavy lysate in 1 mL total volume and incubated with 2% SDS and 2 M urea final concentration in PBS in a final volume of 2 mL for 40 min at 22 °C ...
-
bioRxiv - Biophysics 2020Quote: ... 1 mM EGTA (pH 8.0)) and further purified on a Source 15Q anion exchange column (GE Healthcare) with the application of 50 mL buffer I (20 mM Tris ...
-
bioRxiv - Cell Biology 2022Quote: ... The clarified lysates were incubated with glutathione sepharose (1 ml bed volume; GE Healthcare #17-0756-01), washed and eluted by the addition of 2 x 1.5 ml of 10 mM reduced L-glutathione (Sigma #T6066 ...
-
bioRxiv - Biochemistry 2022Quote: ... and loaded at 1 mL/min onto a Ni-NTA column (HisTrap HP, 5 mL; GE Healthcare) that had been previously equilibrated with Ni-NTA Buffer (NB ...