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Citations for GE Life Sciences :
1751 - 1800 of 1989 citations for Biotin Z Antibody Internalization Kit rabbit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... Hybridization probes were labeled with α[32P]dCTP by random priming (Multiprime DNA labeling kit; GE Healthcare). Hybridizations were performed at 65°C in Rapid-Hyb buffer (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was isolated from the cells using an Illumina RNAspin Mini RNA isolation kit (GE Healthcare). The samples were treated with DNase I to remove any contaminating genomic DNA ...
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bioRxiv - Microbiology 2022Quote: ... for 2 hours at room temperature and visualisation using enhanced chemiluminescence (ECL kit, GE Healthcare Life Sciences). Data were analysed using the gel analysis package in FIJI.
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bioRxiv - Molecular Biology 2024Quote: Total RNA from cell samples was extracted using the Illustra RNAspin kit with DNaseI treatment (GE Healthcare). RT-PCR was performed with 100 ng of total RNA using SuperScript III One-Step RT-PCR System (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Labeled probes were then purified trough a G50 column from the Illustra MicroSpin G50 kit (GE Healthcare) and precipitated overnight with Salmon sperm DNA (Sigma) ...
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bioRxiv - Cancer Biology 2023Quote: PCR products were purified using an ExoSAP-IT PCR Purification Kit (US78200, GE Healthcare, Piscataway, NJ, USA) and sequencing reactions were performed with the Big Dye Terminator V1.1 Kit (4336805 ...
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bioRxiv - Cell Biology 2023Quote: ... followed by reverse transcription of mRNA into cDNA using a First-Strand cDNA Synthesis Kit (GE Healthcare). To isolate the Nb repertoire ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were washed 3 times for 5 min in TBST before incubation for 1 h at room temperature with secondary antibodies: donkey anti-mouse (1:5000; GE Healthcare, NA931-1ML), or donkey anti-rabbit (1:5000 ...
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bioRxiv - Cell Biology 2022Quote: ... The signal of the secondary antibody was detected by using the detect solution mixture (1:1) (ECL Prime Western Blotting System, GE Healthcare Life Science) and a luminescent detector (Amersham Imager 600 ...
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bioRxiv - Cell Biology 2019Quote: ... Blots were then washed and incubated with horseradish peroxidase-conjugated secondary antibodies and were developed using either ECL Prime (GE Healthcare, Piscataway, NJ) or WesternBright Sirius horseradish peroxidase substrate (Advansta) ...
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bioRxiv - Cell Biology 2021Quote: ... They were then incubated with a horseradish peroxidase-conjugated secondary antibody and proteins were detected using enhanced chemiluminescence (GE Healthcare Life Sciences, RPN2209). Densitometry analysis of bands was performed using Image J software.
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bioRxiv - Microbiology 2022Quote: ... Antibodies were detected using an ECL detection reagent and imaged on the ImageQuant LAS 4000 or Amersham Imager 600 (GE Healthcare Life Sciences). All quantitative analysis derived from western blotting was performed using densitometry analysis in ImageJ.
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bioRxiv - Immunology 2022Quote: ... The proteins were harvested from the supernatant of cell cultures and purified by HisTrap Excel (for his-tagged proteins) or Protein G (for antibodies) columns (GE Healthcare Life Sciences). RHMS-1 (trimer) ...
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bioRxiv - Cancer Biology 2022Quote: ... cell lysates were immunoprecipitated with the indicated antibodies for 3 h at 4 °C and then incubated with protein G (GE Healthcare Life Sciences) for 2 hrs at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: We used a modified version of a previously described protocol (41). Briefly, we immobilized monoclonal 1D4 antibody (generously provided by D. Oprian, Brandeis University) onto Sepharose-4B (GE Healthcare Life Sciences). All steps were done in a darkroom under dim red-light illumination ...
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bioRxiv - Molecular Biology 2019Quote: ... characterization of an N-terminal Aβ peptide DAE-EG (DAEFRHDSGYSGKQKSRNEGKGGC) binding to anti-mouse captured antibodies from hybridoma supernatants was performed using a BIAcore T-200 instrument (GE Healthcare, Marlborough, MA) [36] ...
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bioRxiv - Molecular Biology 2021Quote: ... Antibodies were affinity-purified from filtered cultured supernatant on a High-Trap protein G column (GE Healthcare, Chicago, USA, Cat.Nr 17-0404-01).
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bioRxiv - Microbiology 2021Quote: ... membranes were incubated with horseradish peroxidase-coupled secondary antibodies and imaged using chemiluminescent substrate on an Amersham Imager 600 (GE Healthcare, Chicago, Illinois).
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bioRxiv - Molecular Biology 2021Quote: ... NET2 buffer was added to a final volume of 1 mL with 20 µL of Glutathione Sepharose 4B or SF3b155a-antibody conjugated with Protein G Sepharose (GE Healthcare Life Sciences) and incubated at 4°C for 3 h ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed to remove unbound phages and then probed with anti-M13-HRP secondary antibody (1:5000, Cytiva [formerly GE Healthcare], Marlboro, MA) followed by TMB as noted above.
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bioRxiv - Immunology 2021Quote: ... containing 5% non-fat milk (5% milk TBS-N) for 1 hour and probed with primary and HRP-conjugated secondary antibodies (GE Healthcare, 1:3000) diluted in 5% milk TBS-N for 1 hour at room temperature or overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was then incubated in PBST containing a 1:2500 diluted secondary antibody (horseradish peroxidase-linked anti-mouse IgG from sheep; GE Healthcare, Cat. NA931V). After 3 rounds of 5-min wash with PBST ...
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bioRxiv - Cancer Biology 2024Quote: ... Detection and quantification were performed using HRP-coupled secondary antibodies and and an Image Quant LAS4000 chemiluminescence-based detection system (enhanced chemiluminescence) (GE Healthcare Life Science).
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bioRxiv - Microbiology 2023Quote: ... Lysates were incubated with antibodies for one hour at 4°C in an overhead tumbler before immune complexes were retrieved by protein G (GE Healthcare or abcam) or A Sepharose (GE Healthcare) ...
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bioRxiv - Immunology 2023Quote: ... membranes were washed 3x in PBS-T and then incubated with appropriate secondary antibody conjugated with horseradish peroxidase (GE Healthcare, Chicago, IL, USA). Chemiluminescence was accomplished using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed 3 times 10 minutes each with TBS-T and then incubated with the corresponding IgG secondary antibodies horseradish peroxidase-conjugated (GE Healthcare Life Sciences) at 1:1000 for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... IgG and target specific antibodies (anti-UBAP2L or anti-PLK1) as well as protein G sepharose 4 Fast Flow beads (GE Healthcare Life Sciences) were used ...
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bioRxiv - Biochemistry 2023Quote: ... The antibodies raised against each AtCYT6 domain were purified from the sera using CNBr-activated Sepharose 4 Fast Flow beads (GE Healthcare, Uppsala, Sweden) with immobilized recombinant AtCYT6-NTD and AtCYT6-CTD according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... H2AX (total) (#2595S; Cell Signaling technology]. The membranes were extensively washed with 1% TBST followed by incubation with anti-isotype secondary antibody (GE Healthcare) conjugated with horseradish peroxidase in 5% skimmed milk at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... LC3 and other proteins were determined from cell extracts by quantitative immunoblots analysis using chemiluminescence detection of horseradish peroxidase-conjugated secondary antibodies on the GE Image-Quant LAS-4010 (GE Healthcare, Waukesha, WI). Image-Quant software (GE ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was isolated from the time point pooled salivary glands using Illustra RNAspin Mini kit (GE Healthcare Lifesciences) protocols ...
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bioRxiv - Immunology 2019Quote: ... HRP conjugates bound to antigen were detected and visualized by using an ECL detection kit (GE Life Sciences).
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bioRxiv - Evolutionary Biology 2020Quote: ... Total RNA was extracted from samples using the illustra RNAspin Mini RNA Isolation kit (GE Healthcare, Buckinghamshire, UK) according to the manufacture’s protocol ...
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bioRxiv - Immunology 2022Quote: ... HRP conjugates bound to antigen were detected and visualized by using an ECL detection kit (GE Life Sciences).
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bioRxiv - Biochemistry 2020Quote: ... Membranes were washed in 1X TBS and developed using the ECL Select Western Blot Detection kit (GE Healthcare) for chemiluminescent detection (Omega Lum G) ...
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bioRxiv - Microbiology 2019Quote: ... The products of PCR were purified from an agarose gel by using a DNA purification kit (GE Healthcare) and were cloned into the pGEM-T Easy Vector following supplier’s instructions ...
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bioRxiv - Immunology 2019Quote: ... HRP conjugates bound to antigen were detected and visualized by using an ECL detection kit (GE Life Sciences).
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bioRxiv - Molecular Biology 2020Quote: Total RNA was extracted from 108 epimastigotes from exponentially growing cultures using Illustra RNAspin Mini kit (GE Healthcare). cDNA synthesis was done using SuperScript II Reverse Transcriptase kit first-strand synthesis (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... Proteins were purified on the Ni resin using a commercial buffer set (His Buffer Kit, GE Healthcare, USA). In brief ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were purified for sequencing by Illustra GFX PCR DNA and Gel Band Purification kit (GE Healthcare). Primers and purified DNA were sent to GATC biotech (Lightrun method ...
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bioRxiv - Cell Biology 2019Quote: ... ECL was performed using EZ-ECL kit (Biological Industries) and imaged on an ImageQuant chemiluminescence camera (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: Method 1 (H-P): Genomiphi™ V2 DNA Amplification Kit (Cytiva illustra™, formerly GE Healthcare Life Sciences) was used for whole genome amplification according to manufacture instruction ...
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bioRxiv - Immunology 2020Quote: Whole-blood leukocyte DNA was extracted using the Nucleon BACC 3 DNA extraction kit (GE healthcare, Buckinghamshire, UK). We genotyped patients using the Illumina OmniExpressExome-8 Bead Chip (Illumina ...
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bioRxiv - Immunology 2020Quote: ... the PCR product was purified with the GFX PCR DNA and Gel Band Purification Kit (GE Healthcare, UK) and eluted in dH2O ...
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bioRxiv - Genetics 2020Quote: ... Total genomic DNA was isolated from whole blood using Illustra blood genomicPrep Mini Spin Kit® (GE Healthcare) and stored at −20°C ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing pure KIT or KIT:SCF dimers were gently concentrated using a 100 kDa MWCO concentrator (GE Healthcare) at 100×g to a concentration of 6.5 mg/mL ...
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bioRxiv - Microbiology 2022Quote: ... Extracted DNA was amplified using the commercial Illustra™ GenomiPhi V2 DNA Amplification Kit (GE Healthcare Life Sciences) to generate sufficient material for shotgun 454 pyrosequencing library preparation ...
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bioRxiv - Microbiology 2022Quote: ... purified after agarose gel electrophoresis using the GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare) and ligated into the linearized pHT315-gfp vector ...
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bioRxiv - Microbiology 2024Quote: ... and the cells were used for RNA extraction using the Illustra RNAspin Mini RNA isolation kit (GE Healthcare) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... The resultant probe was internally labelled with [α-P32] dCTP (BRIT India) using Rediprime labelling kit (GE healthcare) and used for hybridization of the membrane ...