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Citations for GE Life Sciences :
1451 - 1500 of 1936 citations for Biotin Z Antibody Internalization Kit rabbit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Membranes were washed three times in TBST before incubation with HRP-conjugated secondary antibodies for 1hr at room temperature in 2% milk TBST (1/10,000, GE Healthcare).
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bioRxiv - Cancer Biology 2020Quote: ... the membranes were incubated with 10,000-fold diluted peroxidase-linked secondary antibody (NA931V for FLAG-BRCA2 and NA934V for beta-Actin; GE Healthcare) at room temperature for 4 hours ...
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STARCH SYNTHASE 4 is required for normal starch granule initiation in amyloplasts of wheat endospermbioRxiv - Plant Biology 2021Quote: ... Affinity purification of TaBGC1-specific antibodies from the antiserum was performed with a HiTrap NHS-Activated HP column (GE Healthcare), conjugated to TaBGC1 recombinant protein.
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bioRxiv - Plant Biology 2021Quote: ... α-P4 or α-P6 before subsequent addition of secondary horseradish peroxidase (HPR)-conjugated antibodies (1:20.000; Amersham, GE Healthcare). The immunoreaction was developed using the ECL Prime kit (Amersham ...
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bioRxiv - Microbiology 2020Quote: ... The detected proteins were revealed with 1:3,000-diluted horseradish peroxidase (HRP)-conjugated anti-mouse IgG secondary antibody (GE Healthcare) using a Pierce ECL chemiluminescence substrate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Biovar-specific LPS antibodies were purified by standard affinity chromatography as described by the manufacturer (GE Life Sciences, PA USA).
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bioRxiv - Microbiology 2020Quote: ... and the others were incubated with the indicated antibodies overnight at 4°C.Then mixed with the Protein G sepharose beads (GE Healthcare) for 2 h at 4°C.The immunoprecipitates were washed four to six times with the 293T lyses buffer (50 mM Tris-HCl ...
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Rag GTPases and Phosphatidylinositol 3-Phosphate Mediate Recruitment of the AP-5/SPG11/SPG15 ComplexbioRxiv - Cell Biology 2020Quote: ... Chemiluminescence detection of HRP-conjugated secondary antibody was carried out using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare) and X-ray film ...
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bioRxiv - Molecular Biology 2020Quote: ... They were then incubated for an hour at RT with 1:5,000 anti-rat horseradish peroxidase conjugated antibody (GE Healthcare). Protein bands were detected on X-ray film using SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were incubated with the appropriate horseradish peroxidase (HRP)-labeled secondary antibody (1:5000 dilution; GE Healthcare UK Ltd, UK). Protein bands were detected using enhanced chemiluminescence (Luminata Crescendo ...
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bioRxiv - Molecular Biology 2020Quote: ... After washing the membranes four times for 5 min at room temperature in Washing buffer (PBS, 0.1% (w/v) Tween-20) appropriate HRP-coupled secondary antibodies (anti-mouse IgG (NA931, GE Healthcare), anti-rabbit IgG (NA934 ...
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bioRxiv - Systems Biology 2022Quote: ... Immunoblots were carried out with the antibodies listed above and visualized using the ECL Western Blotting Detection Reagents (GE Healthcare). In order to strip antibodies from the blots ...
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bioRxiv - Microbiology 2021Quote: ... Wells were washed and probed with 100 μl/well of a goat α-GST antibody (GE Healthcare Life Sciences, 27457701V) diluted 1:800 and incubated for one hour at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... in TBS before a 1 hour incubation with a 1:5,000 dilution of sheep anti-mouse IgG horseradish perodixase secondary antibody (GE Healthcare) in 5% milk-TBS ...
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bioRxiv - Biochemistry 2021Quote: ... the supernatant was collected and the antibodies were purified on a HiTrap Protein-A column (GE healthcare, Little Chalfont, UK).
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bioRxiv - Biochemistry 2021Quote: ... Polyclonal anti-GST antibody (Carl Roth, 3998.1) was covalently immobilized to all four flow cells of a CM5 sensorchip (GE Healthcare) to a level of 8,000 response units (RU ...
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bioRxiv - Biophysics 2020Quote: The binding affinities of the Fab antibody fragment Fab705 for full-length MgtE were measured using MicroCal iTC200 (GE Healthcare). The full-length MgtE proteins were purified by a similar procedure to that described above but with SEC buffer [buffer B containing 0.05% (w/v ...
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bioRxiv - Biophysics 2020Quote: ... followed by washing and incubation with the secondary antibody anti-mouse IgG linked to HRP (1:1000, #NA931, GE Healthcare).
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bioRxiv - Immunology 2021Quote: ... extracellular domains were expressed in Drosophila S2 cells and purified by immunoaffinity chromatography with LB3.1 antibody followed by Superdex200 (GE Healthcare) size exclusion chromatography as described [65 ...
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bioRxiv - Immunology 2020Quote: ... The fractions containing the antibody were pooled and elution buffer was exchanged for PBS using NAP-25 columns (GE Healthcare). Purified VHH72hFc1X7 in PBS was quantified by absorbance at 280 nm using a Nanodrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were incubated with secondary antibodies conjugated with horseradish peroxidase (HRP) diluted in 5% skim milk in TBS-T (1:10,000, GE healthcare). Labelled proteins were detected via enhanced chemiluminescence (Pierce ECL Western Blotting Substrate ...
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bioRxiv - Cell Biology 2023Quote: ... or with horseradish peroxidase (HRP)-conjugated secondary antibodies followed by a 3-min incubation with a chemiluminescent substrate (GE Healthcare) for ChemiDoc imaging ...
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bioRxiv - Immunology 2023Quote: IgG was purified from plasma samples by separation over a HiTrap Protein A HP Antibody Purification Column (GE Life Sciences) fitted to a GE Life Sciences Akta Purifier 10 HPLC ...
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bioRxiv - Plant Biology 2023Quote: ... was used as secondary antibody together with the Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare; Cat. No. RPN2232) for signal detection.
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated with specific peroxidase-conjugated secondary antibodies [sheep anti-mouse IgG HRP-linked (1:1000; NA931, GE Healthcare) and donkey anti-rabbit IgG HRP-linked (1:2000 ...
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bioRxiv - Physiology 2023Quote: ... the membranes were then washed three times with a PBS 0.3% tween solution and incubated with a horseradish peroxidase-conjugated secondary antibody (GE Healthcare) in PBS 0.3% tween 3% BSA for one hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Antibodies were affinity purified against DLI-1(1-368) coupled to a 1-mL HiTrap N-hydroxysuccinimide column (GE Healthcare) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed with TBS containing 0.1% polysorbate-20 and then incubated in blocking buffer containing horseradish peroxidase-conjugated secondary antibodies (GE Healthcare) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting elutes containing antibodies were applied to a size exclusion chromatography column (Superdex 200 10/300 GL; GE Healthcare) with PBS served as running buffer and a flow rate of 0.5 mL/min (SI Figure 8) ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were incubated for 1 h with horseradish peroxidase (HRP)-conjugated secondary antibody (dilution 1:10,000) and developed with enhanced chemiluminescence (ECL) reagent (GE Healthcare). All western blots were visualized with a LAS4000 Imaging Station (GE Healthcare) ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes incubated with HRP-conjugated secondary antibodies were incubated with Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare #RPN2232) for 5 mins ...
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bioRxiv - Biochemistry 2024Quote: ... The washing step was followed by 30 min incubation with HRP-conjugated Mouse Anti-M13 monoclonal antibody (GE Healthcare, 27942101) diluted in PBST in 1:5000 ratio ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were purified with the glutathione-Sepharose 4B protein chromatography purification kit (GE Healthcare). Total protein was induced by isopropyl-β-D-thiogalactopyranoside ...
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bioRxiv - Cell Biology 2020Quote: ... Whole-genome amplification was performed using the illustra GenomiPhi V2 DNA amplification kit (GE Healthcare). In brief ...
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Pluripotent stem cell SOX9 and INS reporters facilitate differentiation into insulin-producing cellsbioRxiv - Developmental Biology 2021Quote: ... Protein bands were visualized by chemiluminescence using the detection kit (GE Healthcare Europe, Solingen, Germany) on a chemiluminescence imager (INTAS Science imaging ...
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bioRxiv - Genomics 2020Quote: ... We extracted high molecular weight DNA with the Illustra Nucleon PhytoPure kit (GE Healthcare, RPN8511) following the manufacturers protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were visualized using the chemiluminescent substrate of an ECL kit (GE Healthcare – RPN2232). Images were captured on ImageQuant LAS 4000 (GE Healthcare).
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bioRxiv - Neuroscience 2019Quote: ... were transferred to nitrocellulose membranes and processed for immunoblotting using ECL Prime kit (GE Healthcare). Images of immunoblots were captured using ChemiDoc (Bio-Rad ...
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bioRxiv - Physiology 2019Quote: ... Protein concentration was determined using commercially available 2-D quant kit (GE Healthcare Life Sciences). Sample quality was confirmed using NuPAGE® protein gels stained with fluorescent SyproRuby protein stain (Thermo Fisher).
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bioRxiv - Biochemistry 2019Quote: ... ribonuclease A (13.7 kDa) and aprotinin (6.6 kDa) (LMM gel filtration standard kit, GE Healthcare). This calibration curve was then used to estimate the molecular weight of both G6b-B ECD and G6b-B ECD +dp12 in order to determine their polymeric states.
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bioRxiv - Cell Biology 2019Quote: RNA was isolated from F4/80+ macrophages using Illustra RNA Spin Mini kit (GE Healthcare). cDNA was made with High Capacity cDNA kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... RNA isolation was done by using IllustraTM RNAspin Mini kit (GE Healthcare Life Sciences, UK) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Images were acquired using the ECL Advanced Chemiluminescence kit (GE Healthcare Life Sciences ®, UK) according to manufacturer’s protocols and visualized using a LAS500 CCD camera.
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bioRxiv - Genetics 2020Quote: ... Amplification was performed using either Illustra GenomePhi V2 DNA amplification kit (GE Healthcare 95042-266), or REPLI-g single cell kit (Qiagen Cat #150345 ...
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bioRxiv - Microbiology 2021Quote: ... Signals from m5C were developed with an enhanced chemiluminescence (ECL) western blotting kit (GE Healthcare).
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bioRxiv - Cancer Biology 2020Quote: Total RNA was extracted using the Illustra RNA spin Mini Kit (GE Healthcare, Milan, Italy), converted into cDNA with the RevertAid First Strand cDNA Synthesis Kit following manufacturer’s recommendations (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Genomic DNA samples were amplified with the illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare). We designed PCR primers to amplify all coding regions of CUX2 (NM_015267) ...
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bioRxiv - Cell Biology 2022Quote: ... the blots were developed using an enhanced chemiluminescence kit (ECL Prime, GE Healthcare, IL, USA).
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bioRxiv - Plant Biology 2021Quote: ... followed by [α-32P]dCTP-labeling by random priming (Multiprime DNA labeling kit; GE Healthcare).
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bioRxiv - Neuroscience 2020Quote: ... First-strand synthesis of cDNA was performed using First-Strand cDNA synthesis kit (GE Healthcare), according to manufacturer’s instructions ...