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Citations for GE Life Sciences :
1601 - 1650 of 6458 citations for SARS CoV 2 Membrane Envelope Fusion Protein His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... the remaining 10 ml of concentrated 6-His-AdhE in buffer A were loaded onto a HiLoad 26/600 Superdex 200 pg column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Biochemistry 2024Quote: ... The middle two layers were removed for subsequent desalting (using five connected Hi-Trap desalting columns (GE Healthcare, Chicago, IL, USA) on an AKTA Pure FPLC system) ...
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bioRxiv - Developmental Biology 2019Quote: ... 1ml supernatant (2mg protein) was incubated with 30μl Protein G Sepharose (GE Healthcare) for two hours on a rotator at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were pre-cleared using 40 μl of Protein G Sepharose (GE Healthcare), for 1 hour at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Soluble Ig-fused proteins were purified using protein A-Sepharose beads (GE Healthcare). Bound proteins were eluted with 0.1 M glycine (pH 2.5 ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were further incubated with protein A/G-sepharose beads (GE Healthcare) for 2 hours ...
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bioRxiv - Biochemistry 2022Quote: ... Fc-fused ACE2 proteins were purified on HiTrap Protein A column (GE Healthcare) and eluted with 50 mM Tris pH 7.2 ...
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bioRxiv - Neuroscience 2020Quote: ... Placement in the spinal cord was confirmed with a commercially-available ultrasound system (LOGIQ e Vet, GE Healthcare, Fairfield, CT) using a linear array 4-10 MHz transducer (8L-RS) ...
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Synaptic Zn2+ potentiates the effects of cocaine on striatal dopamine neurotransmission and behaviorbioRxiv - Neuroscience 2021Quote: ... Slides were dried under stream of cool air and placed on BAS-IP TR 2025 E Tritium Screen (GE Healthcare) for 5-days and imaged using a phosphor imager (Typhoon FLA 7000 ...
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bioRxiv - Bioengineering 2020Quote: Vascular patency was checked using the color doppler mode of an ultrasonic device (LOGIQ e Ultrasound; GE Healthcare, Fairfield, USA). Angiography was also performed with a bolus intravenous injection of 2 ml/kg iohexol (Omnipaque™ ...
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bioRxiv - Biophysics 2020Quote: ... Kif14 constructs were finally separated from the MBP tag by size-exclusion chromatography on a superpose 6 10/300 (GE Healthcare) column equilibrated with BRB80 buffer (80 mM Pipes (pH 6.8) ...
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bioRxiv - Microbiology 2019Quote: ... Plasmid pGEX-dosR expressing DosR with an N-terminal GST-tag was generated by cloning PCR-derived dosR ORF fragment between BamHI and XhoI sites of pGEX 4T-1 (GE Healthcare) as described for pGEX-phoP (49) ...
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bioRxiv - Biophysics 2019Quote: ... 5 mg/mL of purified rPrP90-231 fused to an N-terminal six-histidine tag was immobilized in a nickel-loaded HisTrap HP affinity column (GE Healthcare). DNA library was added to the resin ...
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bioRxiv - Biochemistry 2020Quote: ... The hexahistidine plus yeast sumo tag was removed by ulp1 protease digestion followed by a second step HisTrap column (GE Healthcare). The target protein was further purified using MonoQ and Superdex G75 columns (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... The 8 × His tag was removed by TEV protease and tag-free WDR522-334 was further purified by gel filtration using a Superdex 75 (10/300) increase column (GE Healthcare) in 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... It was first purified by a glutathione-affinity step and then subsequent to tag removal following incubation with 3C protease further purified on a MonoS ion-exchange (GE Healthcare) column equilibrated into 25 mM MES pH 6.5 ...
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bioRxiv - Biochemistry 2021Quote: ... Purification of enzyme having N-terminal hexahistidine tag was done by Ni-NTA affinity chromatography using an Akta pure FPLC system (GE Healthcare). Equilibration buffer was 50 mM tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2021Quote: Full-length Pak1 constructs (wild type and catalytically inactive) and full-length Sts5 constructs (wild type and phosphomutant) were cloned into pGEX6P1 (GST tag) vector (GE Healthcare) for expression in BL21 DE3 Escherichia coli ...
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bioRxiv - Biochemistry 2020Quote: ... The GST tag was cleaved overnight at 4°C by the addition of PreScission Protease (~100 μL at 76 mg/mL) (GE Healthcare) and the eluted ATAD2B bromodomain protein was concentrated to a total volume of approximately 3 mL ...
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bioRxiv - Microbiology 2020Quote: ... Binding was detected by incubating the plates sequentially with either mouse anti-Histidine Tag antibody (MCA1396, Abd Serotec) followed horseradish peroxidase (HRP)-linked anti-mouse IgG (1/2000, NXA931, GE Healthcare) or Streptavidin-HRP (554066 ...
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bioRxiv - Biophysics 2021Quote: ... The polyhistidine tag was removed by overnight incubation with TEV protease and further purified by Superdex-75 size-exclusion column (GE Healthcare) in HPLC buffer (20 mM HEPES ...
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bioRxiv - Cell Biology 2020Quote: ... N571 and N442 in frame with N-terminal tag glutathione S-transferase (GST) in pGEX-6P-3 expression plasmids (GE healthcare) were expressed in BL21-CodonPlus-RP E ...
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bioRxiv - Cell Biology 2021Quote: ... Full-length human αSyn cDNA containing an N-terminal myc epitope-tag was inserted into a modified pGEX-KG vector (GE Healthcare), after a TEV protease recognition site to allow cleavage from GST (myc-αSyn contains an extra N-terminal glycine after cleavage with TEV protease) ...
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bioRxiv - Biochemistry 2022Quote: ... Further polishing of the complex prior SEC and after tag cleavage with PreScission protease was carried out by loading onto a cation-exchange (CE) Resource-S column (GE Healthcare) equilibrated in 10mM HEPES (pH 7.5) ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was passed through the HisPur resin to remove eGFP-His8 tag and TEV protease and further purified using Superdex 200 Increase 10/300 gel filtration column (GE Healthcare) in SEC buffer containing 0.06% UDM /0.2% DM ...
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bioRxiv - Immunology 2022Quote: ... the proteins were treated with HRV3C protease to cleave the His6-NusA-tag and were further purified by a combination of chromatography using HiTrap Heparin (GE Healthcare), Hitrip SP (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... the proteins were treated with HRV3C protease to cleave the His6-NusA-tag and were further purified by size-exclusion chromatography on Superdex 75 Increase 10/300 (GE Healthcare) column ...
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bioRxiv - Molecular Biology 2022Quote: ... Ube2V2 and Ube1 were expressed with N-terminal GST-tag followed by a TEV protease cleavage site and purified via glutathione sepharose resin (GE Healthcare) equilibrated in 50 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2022Quote: ... with an N-terminal gp67 signal peptide for secretion and a C-terminal 6×His tag for purification was expressed using Hi5 cells and purified by Ni-NTA resin followed by gel filtration chromatography (GE Healthcare) in HBS buffer (10 mM HEPES ...
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bioRxiv - Cell Biology 2019Quote: ... Human CFP1 and truncation constructs were subcloned into the pGEX-6P-1 vector encoding an N-terminal glutathione S-transferase (GST) tag (GE Healthcare) prior to expression in Origami cells ...
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bioRxiv - Biochemistry 2019Quote: ... The final size-exclusion chromatography after affinity purification and tag removal was performed using Superdex75 16/60 HiLoad and Superdex75 increase 10/300GL columns (GE Healthcare) with 20 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... All recombinant pilins were produced with an N-terminal histidine tag for immobilized nickel affinity purification using 1ml HisTrap columns (GE Healthcare) per the manufacturer’s protocol ...
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bioRxiv - Biophysics 2020Quote: ... The Smt3 tag was cleaved off before further purification by a HiLoad 16/600 Superdex 75 size exclusion column (GE Healthcare) in NMR buffer (20 mM sodium phosphate pH 6.5 ...
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bioRxiv - Plant Biology 2019Quote: The construct of full length AtMLKL2 (residues 1-711) with N-terminal GST tag was cloned into the pGEX-6P-1 vector (GE Healthcare), and was expressed in Escherichia coli strain BL21(DE3 ...
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bioRxiv - Molecular Biology 2021Quote: ... Binding was detected by incubating the plates sequentially with either mouse anti-Histidine Tag antibody (MCA1396, Abd Serotec) followed by horseradish peroxidase (HRP)-linked anti-mouse IgG (1/2000, NXA931, GE Healthcare) or Streptavidin-HRP (554066 ...
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bioRxiv - Genomics 2021Quote: ... The cleaved fused 6His-SUMO-tag was removed from the FLAG-MCP by reloading the sample onto the second HisTrap column (GE Healthcare). The FLAG-MCP was further purified by chromatography using a HiTrap Heparin SP column (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleaved MBP tag and TEV protease were removed by anion exchange chromatography using a Mono Q 5/50GL (GE Healthcare) column pre-equilibrated with buffer-B ...
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bioRxiv - Biochemistry 2023Quote: The GSTs contained an N-terminal hexahistidine tag enabling their purification by immobilized metal affinity chromatography (IMAC) using a Ni-IMAC column (GE Healthcare). The supernatant fraction from the centrifugation was loaded onto the column equilibrated with binding buffer (20 mM sodium phosphate ...
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bioRxiv - Plant Biology 2024Quote: ... the cleaved tag from the 6xHis-MBP-Pwl2 purification was removed from the solution using a MBPTrap HP dextrin sepharose column (GE Healthcare) and subsequently eluted using SEC buffer supplemented with 10mM maltose.
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bioRxiv - Plant Biology 2024Quote: ... the untagged protein was separated from cleaved tags and the 6xHis-tagged 3C protease by affinity chromatography using 5 ml HisTrap HP NTA column (GE Healthcare). If proteins were also tagged with MBP ...
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bioRxiv - Biochemistry 2023Quote: ... the His6-NusA-tag was removed using the HRV3C protease and was further purified by SEC on Superdex 75 Increase 10/300 (GE Healthcare) column in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2023Quote: ... Affinity-purified proteins were incubated with HRV-3C protease to remove purification tags and subsequently purified by size-exclusion-chromatography on a Superose 6 Increase column (GE Healthcare) in TBS
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bioRxiv - Biochemistry 2023Quote: ... the protein was treated with HRV3C protease to cleave the His6-NusA-tag and were further purified by a series of chromatography purification using HiTrap Heparin (GE Healthcare), Hitrip SP (GE Healthcare ...
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bioRxiv - Plant Biology 2022Quote: ... diluted 1:10,000 in 2% TBST with 2% Blocking (GE Healthcare), was applied for 1 h.
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bioRxiv - Microbiology 2024Quote: ... 2 µMnt ssDNA (Ovio 45) and 2 mM ATP (GE Healthcare) in a 40 µL reaction solution containing 10 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cancer Biology 2021Quote: ... or Amersham Protan nitrocellulose membranes (#10600001 GE Healthcare Life Sciences). Western blot analysis was performed with primary antibodies recognizing ...
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bioRxiv - Cell Biology 2021Quote: ... mRNA was hybridized onto the Hybond-N+ membrane (GE Healthcare). After crosslinking spotted mRNA to membrane using Stratalinker 2400 UV Crosslinker ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was transferred to a Hybond-N+ membrane (GE Healthcare) at 4mA/cm2 for 45 min and fixed by UV-crosslinking ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and transferred onto a Hybond N+ nylon membrane (GE Healthcare). Probe labelling ...
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bioRxiv - Cancer Biology 2022Quote: ... and electro-blotted on nitrocellulose membranes (GE Healthcare Life Science). Later ...