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Citations for GE Life Sciences :
1501 - 1550 of 6458 citations for SARS CoV 2 Membrane Envelope Fusion Protein His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... Gels were electroblotted on N+ nylon membranes (GE Healthcare), hybridized at 42°C using a SpCas9 ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were exposed using the ECL method (GE Healthcare) as per manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... and transferred onto nitrocellulose membranes (GE Healthcare Life Sciences). The membranes were then blocked with 5% skim milk in 50 mM Tris-HCl [pH 7.6] ...
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bioRxiv - Neuroscience 2022Quote: ... and blotted onto nitrocellulose membranes (GE Healthcare, Chicago, USA). The membranes were blocked in 5% skim milk (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were blotted onto a Hybond membrane (GE Healthcare) in 20X SSC ...
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bioRxiv - Cell Biology 2022Quote: ... and transferred to 0.2 µm nitrocellulose membrane (GE Healthcare) using semi dry transfer ...
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bioRxiv - Plant Biology 2022Quote: ... A cellulose acetate membrane filter (GE Healthcare Life Sciences) was placed in a slot blot apparatus (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... followed by transferring to nitrocellulose membrane (GE Healthcare Lifesciences) using the Mini-PROTEAN Tetra Cell (Bio-Rad Laboratories) ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was transferred to Hybond-N+ membranes (GE Healthcare) by capillarity using 20× SSC as transfer buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were prehybridized in Rapid-hyb Buffer (GE Healthcare) for 30 minutes at 65°C ...
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bioRxiv - Molecular Biology 2022Quote: ... unstained gels were transferred to Hybond+ membranes (GE Healthcare) followed by UV crosslink with 120 mJ of UV light at 254 nm ...
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bioRxiv - Molecular Biology 2022Quote: ... unstained gels were transferred to PVDF membranes (GE Healthcare) and probed with protein-specific antisera against RpoB (1:10,000 dilution ...
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bioRxiv - Immunology 2022Quote: ... and transferred to nitrocellulose membranes (0.2 μm) (GE Healthcare). The following antibodies were used for detecting SARS-CoV-2 S ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were developed via chemiluminescence [ECL Select (GE Healthcare)].
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bioRxiv - Microbiology 2023Quote: ... electro-blotted on polyvinylidene fluoride (PVDF) membrane (GE Healthcare) and incubated for 1 hr in 1% sodium salicylate solution (Sigma) ...
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bioRxiv - Developmental Biology 2023Quote: ... Blotting was performed onto a nitrocellulose membrane (GE Healthcare) using a Bio-Rad wet blot system ...
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bioRxiv - Microbiology 2023Quote: ... and transferred to Hybond ECL nitrocellulose membrane (GE Healthcare). Following blocking for 1hr at room temperature in TBS-Tween 20 (0.1% ...
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bioRxiv - Cancer Biology 2023Quote: ... and transferred to a nitrocellulose membrane (GE Healthcare, 10600003) as described [22] ...
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bioRxiv - Microbiology 2023Quote: ... 90V onto nitrocellulose membranes (GE Healthcare, Chicago, IL USA). The blocked membranes were probed with either rabbit polyclonal MMTV anti-Gag antibody (Rockland Immunochemicals ...
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bioRxiv - Microbiology 2023Quote: ... and transferred to a nitrocellulose membrane (GE Healthcare, 10600004). Following blocking with 5% milk for 1 h at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... electroblotting was performed onto Hybond N+ membrane (GE healthcare). Hybridization was performed using T4 PNK end labelled oligonucleotides with [γ-P32] ATP in hybridization buffer (Ultrahyb buffer-Invitrogen).
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bioRxiv - Molecular Biology 2023Quote: ... followed by transfer onto nitrocellulose membrane (Protran, GE Healthcare) and probed with Tubulin (55kDa ...
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bioRxiv - Neuroscience 2023Quote: ... 4°C onto 0.2uM PVDF membranes (GE Healthcare Biosciences). After transfer ...
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bioRxiv - Biochemistry 2023Quote: Western blot was performed on PVDF membranes (GE Healthcare) on a semi-dry system using Bjerrum Schafer-Nielsen transfer buffer (48 mM Tris ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were exposed to ECF substrate (GE Healthcare #1067873) for visualization in a UVP BioSpectrum 500 Imaging System.
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bioRxiv - Molecular Biology 2023Quote: ... followed by wet transfer onto nitrocellulose membrane (GE Healthcare) in carbonate transfer buffer (3 mM Na2CO3 ...
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Activation of XBP1s attenuates disease severity in models of proteotoxic Charcot-Marie-Tooth type 1BbioRxiv - Neuroscience 2024Quote: ... and gels were transferred onto nitrocellulose membrane (GE Healthcare). Membranes were blocked with 5% milk (milk powder/ 1X PBS-Tween 0,1% ...
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bioRxiv - Genomics 2024Quote: ... transferred onto a Hybond-C nitrocellulose membrane (GE Healthcare), then blocked with TBST containing 5% (w/v ...
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bioRxiv - Neuroscience 2024Quote: ... and transferred to nitrocellulose membrane (AmershamTM ProtranTM, GE Healthcare). After transfer ...
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bioRxiv - Molecular Biology 2024Quote: ... and then transferred onto polyvinylidene difluoride membranes (GE Healthcare) using wet tank blotting (25 mM Tris ...
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bioRxiv - Cancer Biology 2024Quote: ... and transferred to nitrocellulose membranes (GE Healthcare, Cat#: 10600004). Membranes were blocked in 5% nonfat dry milk (Giant brand ...
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bioRxiv - Plant Biology 2024Quote: ... A cellulose acetate membrane filter (GE Healthcare Life Sciences) and 3 filter papers (BioRad ...
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bioRxiv - Biochemistry 2020Quote: ... The hexahistidine plus MBP tag was removed by TEV protease digestion followed by a second step HisTrap column (GE Healthcare). Protein was further purified using Q and Superdex G75 columns (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... at 4 °C to remove the SBP-tag and polished by size exclusion chromatography on a Superdex 200 Increase 3.2/300 column (GE Healthcare) equilibrated in 50 mM HEPES-NaOH ...
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bioRxiv - Biochemistry 2020Quote: ... The His6-tag-containing FdR9 was purified by affinity chromatography on an Äkta prime FPLC system (GE Healthcare, Freiburg, Germany), using a 5 mL HisTrap column (GE Healthcare) ...
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bioRxiv - Biophysics 2021Quote: ... The cleaved His6-MBP-tag was removed by gel filtration using a HiLoad 16/60 Superdex 200 column (GE healthcare) equilibrated in size exclusion buffer (20 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... The GST tag of N-terminal GST-6xHis-RhoGAP domain was removed by addition of PreScission protease (GE healthcare Lifesciences) directly to the glutathione beads followed by incubation overnight at 4°C to obtain His-tagged RhoGAP ...
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bioRxiv - Immunology 2020Quote: ... to remove the GST tag and further purified by gel filtration (SuperoseTM 6 prep grade XK 16/70; GE Healthcare). To prepare for crystallization trials ...
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bioRxiv - Microbiology 2024Quote: ... The eluted Cas1 without tag was concentrated and further purified by gel filtration chromatography (Superdex200 10/300 GL, GE Healthcare) in buffer B containing 10 mM Tris pH 8.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1,000 μg of proteins were prepared and pre-cleared with 20 μl of protein A-Sepharose and protein G-Sepharose beads mixture (GE Healthcare). 2 μl of (HA ...
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Stress response in Entamoeba histolytica is associated with robust processing of tRNA to tRNA-halvesbioRxiv - Microbiology 2021Quote: ... 20 μg RNA samples were separated on a denaturing 12% polyacrylamide gel and transferred to a membrane (Amersham™ Hybond™ -N+ Membrane, GE Healthcare). Probe DNA was 5′-end labeled by PNK reaction using γ-[32P]-ATP and hybridized with the membrane in perfectHyb buffer (Sigma ...
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bioRxiv - Plant Biology 2022Quote: ... strain BL21 as described previously,20 using the GST gene fusion system for heterologous expression and affinity purification (GE Healthcare). DNA templates for the cystatins were synthesized as g-blocks (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2020Quote: ... coli BL21 and were purified by immunoprecipitation with glutathione sepharose 4B beads (Cat: 45-000-139, GE Healthcare). Each reaction with 30 μl final volume contained 50 ng Wheat E1 protein ...
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bioRxiv - Molecular Biology 2022Quote: ... coli similar to the GST-MS2 procedure [20] and then purified on a glutathione-Sepharose column (GE Healthcare). Following elution ...
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bioRxiv - Biophysics 2021Quote: ... The purification was done by purifying these two supernatants with 1 M NaCl containing Tris buffer using Hi-Trap Q-FF anion exchange column of ÄKTA Pure (GE healthcare). To maintain the oxidation activity of DsbA 0.3% H2O2 was added to the purified protein for overnight ...
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bioRxiv - Cell Biology 2020Quote: ... The clear supernatant layer was collected and run over five serially connected Hi-Trap desalting columns (GE healthcare, 17-1408-01) in buffer A supplemented with 2 mM DTT and 14% glycerol ...
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bioRxiv - Microbiology 2021Quote: ... Further purification was carried out by size exclusion chromatography using a Superdex™ 200 Hi Load 16/60 column (GE Healthcare). Proteins were eluted with SEC buffer (25 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... the buffer was supplemented with 30 mM imidazole and an additional 100 mM NaCl and passed through a 1 ml Hi-Trap Chelating column (GE Healthcare) to capture the N-terminal fragment that was cleaved by the TEV protease ...
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bioRxiv - Biophysics 2019Quote: ... Sample was further purified from free labelled peptide and Sortase by size-exclusion chromatography using a Hi/Load 16/600 Superdex 75 column (GE Healthcare) previously equilibrated with ZapA storage buffer ...
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Ouabain enhances cell-cell adhesion mediated by β1-subunits of the Na+,K+-ATPase in CHO fibroblasts.bioRxiv - Cell Biology 2019Quote: Total extract of CHO cells expressing the β1His6 construct were immobilized with nickel-nitrilotriacetic acid beads (Ni+-NTA, His Trap FF column; GE Healthcare) equilibrated with 10 ml of RIPA buffer containing protease inhibitors ...