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Citations for GE Life Sciences :
1501 - 1550 of 2093 citations for Anti Syndecan 4 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The anti-mouse M13-HRP (GE Healthcare Cat# 27942101) was added at 1:5000 dilution for 1hr at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... and goat-anti-rabbit were obtained from GE HealthCare/Amersham ...
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bioRxiv - Immunology 2024Quote: ... or peroxidase-conjugated anti-rabbit IgG (#NA934; GE Healthcare), were added as the secondary antibodies to the buffer in which the membrane was soaked ...
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bioRxiv - Biochemistry 2023Quote: ... the secondary mAB anti-mouse (sheep, GE Healthcare, LNA931V) and developed using Clarity Western ECL Substrate (Bio-Rad Laboratories ...
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bioRxiv - Physiology 2024Quote: ... and sheep anti-mouse (GE Healthcare #NA931V, 1:1000). Protein bands were detected using Chemiluminescent Substrate (Thermo Fischer ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were washed with 1x TTBS and placed in a 1:25000 secondary HRP-conjugated IgG anti-rabbit or anti-mouse solution (GE Healthcare®, Sweden) according to the primary antibody datasheet specifications ...
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bioRxiv - Biochemistry 2019Quote: ... Metal chelate chromatography was performed at 4 °C using an IMAC zinc-Sepharose 6 Fast Flow column (2.6 × 20 cm, GE Healthcare Life Sciences, Amersham, UK) equilibrated with 1×PBS pH 6.0 ...
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bioRxiv - Biochemistry 2020Quote: ... lysates were incubated overnight at 4°C with 400 μl of Glutathione Sepharose 4B per l of expression culture (GE Healthcare GE17-0756-01) with gentle rotation ...
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bioRxiv - Microbiology 2021Quote: ... Purified 6x-His-SUMO or 6x-His-SUMO-Pat1 were coupled to NHS-activated Sepharose 4 fast flow resin (GE Healthcare, 17-0906-01) in ligand coupling buffer (200 mM NaHCO3 ...
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bioRxiv - Cancer Biology 2020Quote: ... Western blots were visualized and quantified with enhanced chemiluminescence and imaging on a Fuji LAS 4 000 image station (GE Healthcare, Chicago, IL, US)4.
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bioRxiv - Neuroscience 2022Quote: ... and 500 μg of total protein from the supernatants was precleared with Protein G Sepharose 4 Fast Flow (GE Healthcare, catalog #GE17-0618-01) for 2 h at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... 20 µg of each sample was loaded onto a 4-20% Gel (Bio Rad, Hercules, CA) for electrophoresis and transferred to a PVDF membrane (GE Healthcare Amersham, Chicago, IL). For cryba4 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... the remainders of the lysates were nutated with anti-Cdc15 polyclonal antibody (VU326) (Roberts-Galbraith et al., 2009) for 1 hr at 4°C followed by incubation with protein A sepharose (GE Healthcare; 17-5280-04) for 30 min at 4°C ...
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bioRxiv - Evolutionary Biology 2019Quote: ... on each FTA punch or on 2 µL or 4 µL of DNA solution using the Illustra GenomiPhi V2 DNA Amplification kit (GE Healthcare Life TM Sciences). Amplified DNA was purified with the SigmaSpin Sequencing reaction Clean-up (Sigma-Aldrich) ...
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bioRxiv - Genomics 2021Quote: ... #S0282).[1] Reverse transcription was performed in 1.5 mL microfuge tubes under end-over-end rotation using a modified Reverse Transcription mix (1x Maxima H-RT buffer, 4% Ficoll PM-400 (GE Healthcare, #17-0300-05), 3 mM MgCl2 (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 4000 rpm at 4°C for 20 minutes in order to determine the total protein concentration through NanoVue spectrophotometer (GE Healthcare, Chicago, IL, USA). A total of 40 µg protein was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Biophysics 2020Quote: ... SEC was conducted at 4°C using a Superdex 200 5/150 column on an AKTA Pure System (GE Healthcare Life Sciences, Marlborough, MA) with a flow rate of 0.15 mL/min and 0.5 s exposures ...
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bioRxiv - Bioengineering 2020Quote: ... depending on machine availability and the type of imaging performed: LOGIQ Book XP with 8L-RS 4-12 MHz linear array probe (GE Healthcare, Milwaukee, WI, USA), Aixplorer with L14-5 probe (Supersonic Imagine ...
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bioRxiv - Biophysics 2023Quote: ... The cleared lysate was supplemented with CaCl2 to a final concentration of 2 mM and then incubated for 1 h at 4 °C with washed Sepharose 4B Calmodulin beads (GE Healthcare #17-0529-01) in a spinning rotor ...
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bioRxiv - Biophysics 2023Quote: ... Cdc6 protein was purified from the supernatant by incubating for 1 h at 4 °C with glutathione beads Fastflow (GE Healthcare #17-5132-02). The beads were washed 20 times with 5 ml Cdc6 lysis buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Concentrated media was then subjected to size exclusion chromatography using Sepharose 4 fast flow column connected to the ÄKTA prime system (both GE Healthcare, Chicago, IL, USA) and eluted in PBS with a flow rate of 0.5 ml/min ...
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DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: ... DTX3L and Flag epitope for 1 h at 4°C before Protein G Sepharose beads (30 μL per reaction mixture; GE Healthcare #GE17-0618-01) were added for 12 h ...
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bioRxiv - Microbiology 2020Quote: ... whereas IgG3 antibodies were isolated with a HiTap Protein G High Performance column (GE Healthcare). Antibodies were dialyzed in PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... The antibody was pre-incubated with 20 μl of prewashed protein G Sepharose (GE healthcare) at 4°C for 1 hr ...
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bioRxiv - Biochemistry 2019Quote: ... Antibodies were immobilized using direct immobilization using amine coupling on CM3 sensor chips (GE Healthcare) at ∼5000 RU ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were detected using horseradish peroxidase-coupled secondary antibodies (GE Healthcare Life Sciences, Piscataway, NJ) and ECL Plus or ECL Prime western blotting detection reagents (GE Healthcare Life Sciences) ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were detected using horseradish peroxidase-coupled secondary antibodies (GE Healthcare Life Sciences, Piscataway, NJ) and ECL Plus or ECL Prime western blotting detection reagents (GE Healthcare Life Sciences) ...
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bioRxiv - Biophysics 2019Quote: ... used at 1:5000 dilution and detection with peroxidase-labelled secondary antibodies (GE Healthcare, UK) and ECL Plus (enhanced chemiluminescence ...
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bioRxiv - Cell Biology 2020Quote: ... Chemiluminescence signal from antibody binding was detected using ECL Select detection kit (GE healthcare, GERPN2235) with a ChemiDoc system (BioRad).
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bioRxiv - Bioengineering 2021Quote: ... The antibody secreted into the culture medium was purified using Protein A resin (GE Healthcare).
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bioRxiv - Neuroscience 2020Quote: ... Antibodies were then purified using size exclusion columns (Illustra NAP-5 columns, #17085302, GE Healthcare). The absorption of IgG at 280 nm and Cy3B dye at 559 nm of the reaction products was measured by spectrophotometry (NanoDrop ND-1000 Spectrophotometer) ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies were detected using ECL™ Western Blotting Detection Reagents (RPN2209, GE Healthcare, IL, USA). As a control ...
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bioRxiv - Cancer Biology 2020Quote: ... the antibody-bound membranes were treated with enhanced chemiluminescent western blot detection reagents (GE Healthcare) and visualized with an x-ray film (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... secondary antibodies conjugated with HRP and ECL Prime detection reagents were acquired from GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2020Quote: ... the membranes were incubated with peroxidase-conjugated secondary antibodies at 1: 5000 dilution (GE Healthcare) for 1 hour at room temperature ...
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bioRxiv - Immunology 2022Quote: ... The blots were developed using appropriate HRP-conjugated secondary antibody from GE Healthcare (1:5000) and an enhanced chemiluminescence (ECL ...
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bioRxiv - Molecular Biology 2020Quote: ... the antibody was pre-incubated with 20 μl of prewashed protein G sepharose (GE healthcare) at 4°C for 1 hr ...
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bioRxiv - Neuroscience 2021Quote: ... or with HRP-conjugated secondary antibodies and ECL Prime Western Blotting System (#RPN2106, GE Healthcare) and imaged using a ChemiDoc imaging system (Biorad) ...
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bioRxiv - Bioengineering 2020Quote: ... The conjugated antibody was purified by SEC (Superdex 200 increase 10/300 GL, GE Healthcare, solvent ...
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bioRxiv - Neuroscience 2022Quote: ... The mouse monoclonal antibodies were purified from hybridoma supernatant using protein G columns (GE Healthcare). The specificity of CAST monoclonal antibodies was confirmed by immunohistochemistry with CAST KO mice (56) ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins recognised by the antibodies were visualised using ECL prime (GE Healthcare, Chicago, IL, USA) and digital images were acquired with a LAS4000 mini imager (FUJIFILM Wako Pure Chemical ...
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bioRxiv - Cell Biology 2019Quote: ... Antibody-conjugated proteins were collected by incubating with Protein A Sepharose beads (17528001, GE Healthcare). Beads were then washed 4-5 times with the lysis buffer described above ...
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bioRxiv - Immunology 2019Quote: ... followed by HRP-conjugated secondary antibodies and detection with chemiluminescent substrate solution ECL2 (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... α-rabbit IgG secondary antibody conjugated to HRP (from donkey) was purchased from GE Healthcare and used at 1:5,000 dilution ...
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bioRxiv - Molecular Biology 2020Quote: ... membranes were incubated for two hours with the corresponding HRP conjugated secondary antibody (GE Healthcare). After incubation with the secondary antibody ...
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bioRxiv - Immunology 2020Quote: ... The membrane was washed and incubated with HRP-conjugated secondary antibodies (GE Healthcare, 1:4000) for 1 h at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Antibodies were purified from serum-free culture supernatants using mAb Select SuRe resin (GE Healthcare) followed by rapid desalting using a HiPrep 26/10 Desalting column (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... IgG1 antibodies were isolated from cell supernatant using a HiTrap protein A column (GE Healthcare) and Äkta Pure protein chromatography system (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... we added 100 µl/well of Amersham ECL-HRP linked mouse IgG antibody (GE Healthcare) diluted 1/10,000 ...
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bioRxiv - Cancer Biology 2020Quote: ... incubated with secondary antibodies and visualised using an ImageQuant LAS 4000 imaging system (GE Healthcare). A list of antibodies used for immunoblotting is provided in Supplementary Table1 ...