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Citations for GE Life Sciences :
1601 - 1650 of 2507 citations for 2 4 Methyl 5 thiazolyl ethyl decanoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Lysates were clarified by centrifugation at 50,000 × g for 1 h at 4 °C and the supernatant was used for affinity purification on HisTrap FF columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... The mixture was incubated at room temperature for 4 hours and was purified using gel filtration chromatography (ÄKTA with Superdex-200 increase column; GE Healthcare) equilibrated with storage buffer ...
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bioRxiv - Biophysics 2023Quote: ... 3,000 g × 15 min at 4 °C) and further purified by size-exclusion chromatography (ÄKTA pure system, Superdex-75 Increase 10/300 GL, GE Healthcare). The unfolding and refolding process for GlnBP WT was conducted under the same conditions as described for the GlnBP variants.
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bioRxiv - Biochemistry 2023Quote: ... 4°C) and the soluble proteins with His6 tag were purified on 1ml of nickel phosphate loaded sepharose beads (GE Healthcare). Bound proteins were washed in lysis buffer with 25mM imidazole then with 400mM Imidazole ...
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bioRxiv - Biophysics 2023Quote: ... Q7 or Q20 at a concentration of ~4-7mg/ml were loaded onto an S200 Increase size-exclusion column (Superdex 200 Increase10/300 GL, GE Healthcare) at a flow rate of 0.5 ml min-1 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell extracts were then incubated over night at 4 °C with appropriate antibodies (1ug/ml) and protein A Sepharose beads (GE Healthcare). After incubation samples were washed five times with lysis buffer and resuspended in 1X Laemmli sample buffer (Bio-Rad cat ...
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bioRxiv - Synthetic Biology 2022Quote: ... The gels were dried (Hoefer GD2000) and exposed for at least 4 days before imaging on a Typhoon FLA 700 (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... The supernatant of the cell lysate was incubated overnight at 4°C on a wheel with Glutathione Sepharose 4B resin (GSTrap 4B, GE Healthcare). After several washes ...
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bioRxiv - Microbiology 2023Quote: ... The clarified lysate was incubated (overnight, at 4 °C, with gentle agitation) with 1 ml bed volume of GSH-Sepharose (GE Healthcare). Next ...
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bioRxiv - Biochemistry 2023Quote: ... the N-terminal His tag was cleaved by overnight dialysis with TEV protease at 4 °C and purified by size exclusion chromatography (SEC) (HiLoad Superdex-200 16/600 column, GE Healthcare) in CHIP storage buffer (50 mM HEPES ...
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bioRxiv - Cell Biology 2023Quote: ... actin Ac-74 or Pol II S5P) overnight at +4°C and immune-complexes were collected with 50 μl of protein A sepharose (GE Healthcare) at +4°C for two hours on rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... CpxA31-163-GST was purified at 4°C using a protocol modified from the GST Gene Fusion System Handbook (GE Healthcare). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... HA-UL47-containing protein complexes were immunoprecipitated by incubating extracts with HA-specific antibodies and protein G-coupled sepharose beads (4 Fast Flow, GE Healthcare). The sepharose-bound proteins were analyzed by standard immunoblotting for the presence of HA-UL47 ...
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bioRxiv - Cell Biology 2023Quote: ... hDrp1 was eluted by cleavage from the affinity resin using 30 mM DTT at pH 8 for 48 hours at 4 °C and further purified using anion exchange chromatography (HiTrap Q FF, GE Healthcare) at pH 8 ...
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bioRxiv - Biophysics 2023Quote: ... protein samples were purified by size-exclusion chromatography at 4°C using a Superose 6 10/300 GL column (GE Healthcare), equilibrated with a buffer containing 0.03% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... and indicated prey proteins at a 5X molar excess to tepsin (mATG8 and/or AP-4 β4 appendage) were incubated with glutathione sepharose resin (GE Healthcare) for 2 hours at 4°C in 20 mM HEPES (pH 7.5) ...
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bioRxiv - Cancer Biology 2024Quote: ... Wells were washed twice with room temperature PBS and then 4 fields of view per well imaged on an InCell 6000 (GE Healthcare). Images were segmented with two custom Cellpose[45] models to segment the nucleus (from DAPI channel ...
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bioRxiv - Neuroscience 2024Quote: ... The protein bands were detected by incubating the membrane with primary antibodies (see below) overnight at +4°C and horse radish peroxidase-conjugated secondary antibodies (1:5000, NA934 or NA931, GE Healthcare) for 1 h at RT ...
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bioRxiv - Immunology 2024Quote: ... An affinity matrix was prepared by coupling 50 mg of His-tagged GST-RAP to 20 ml of NHS-activated Sepharose 4 Fast Flow (GE Healthcare), according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... The protein was concentrated using a 100 kDa filter to approximately 1 mL and further purified by size-exclusion chromatography at 4°C using a Superose 6 10/300 GL column (GE Healthcare) equilibrated with buffer containing 0.06% digitonin ...
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bioRxiv - Developmental Biology 2024Quote: ... The GST-fused Ccrk protein was expressed in Escherichia coli strain BL21 (DE3) and purified using glutathione Sepharose 4 B (GE Healthcare). An antibody against Ccrk was obtained by immunizing guinea pigs with purified GST-fused Ccrk.
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bioRxiv - Microbiology 2024Quote: ... The 6ξ-His tag was cleaved for 16 h at 4 °C using 1U of thrombin per 100 µg of total protein (GE Healthcare). The cleaved proteins were then passed through a Ni-NTA column to remove the cleaved 6ξ-His tag and any uncleaved protein ...
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bioRxiv - Plant Biology 2024Quote: ... The mixture was centrifuged at 2,000 g and 4 °C for 10 min and eventually filtered with 0.43 μm organic phase medium (GE Healthcare, 6789-0404). The column was a 100×2 mm Phenomenex Luna 3 μm NH2 (Catalog No. ...
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bioRxiv - Plant Biology 2024Quote: ... 2D gel electrophoresis was performed as described previously (Bhattacharya et al., 2019) using 11 cm Immobiline DryStrips pH 4-7 (GE Healthcare) in Ettan IPGphor3 IEF system (GEHealthcare) ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was incubated first with primary Abs for 24 hours at 4°C and then with HRP–conjugated secondary Abs (GE Healthcare) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... the protein debris was removed by centrifugation (30000×g, 20 min at 4 °C) and the supernatants were applied to His-Trap chelating column (GE Healthcare), washed by refolding buffer and eluted with the same buffer containing 30 mM imidazole ...
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bioRxiv - Biochemistry 2024Quote: ... pH 7.4) using a HiLoad 16/60 Superdex 200 pg gel filtration column operated on an ÄKTA liquid chromatography system (GE Healthcare).
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bioRxiv - Pathology 2024Quote: ... 125 µg of biotinylated PAI-1WT (containing the 4-stabilizing substitutions (20)) was bound to 125 µL of streptavidin-sepharose beads (GE Healthcare). PAI-1-streptavidin-sepharose beads were added to 500 µL of lung supernatant and incubated at 37°C for 1 hour with gentle agitation ...
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bioRxiv - Immunology 2024Quote: ... Cells were filtered through 70 µm cell strainers and then centrifuged at 300xg for 10 minutes at 4°C before being resuspended in 22% Percoll (GE Healthcare) and centrifuged at 950xg for 20 minutes (acceleration=4 ...
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bioRxiv - Immunology 2024Quote: ... Samples were centrifuged at 220xg for 10 min at 4°C and then resuspended in a 22% Percoll solution (GE Healthcare) with a PBS layer floated on top ...
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bioRxiv - Systems Biology 2024Quote: ... overnight at 4°C followed by a 1/10.000 dilution of anti-mouse IgG HRP-linked whole secondary antibody (GE Healthcare NA931), during 1 hour at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... Gels were run for 45 min at 150 V at 4°C before detecting the HEX-label using a Typhoon FLA 9500 instrument (GE Healthcare) at 532 nm wavelength and BPG1 emission filter ...
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bioRxiv - Biochemistry 2024Quote: ... and subsequently concentrated by Amicon Ultra-4 centrifugal filter (MWCO 100 kDa) before being injected into a Superose 6 Column (GE Healthcare). The size exclusion chromatography (SEC ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were clarified by centrifugation at 50,000⍰×⍰g for 1⍰h at 4⍰°C and the supernatant was used for affinity purification on HisTrap FF columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Cellular debris was pelleted by centrifugation for 20 min (18000 × g at 4°C) and GST and MBP fusion proteins were purified using glutathione- Sepharose (GE Healthcare) or amylose (New England Biolabs ...
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bioRxiv - Biochemistry 2024Quote: ... incubated at 4 °C for 30 min and purified using gel filtration (Superdex 200 Increase 10/300 GL) columns (GE Healthcare).
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bioRxiv - Cell Biology 2024Quote: ... Between 5-20 μg of total protein per sample were resolved by SDS-PAGE (4-20% gels) and transferred to Amersham Hybond P 0.45 PVDF blotting membranes (GE Healthcare, 10600023). Membranes were blocked overnight at 4°C in 5% skim milk powder or 5% BSA in PBS 0.1% Tween when working with phospho-specific antibodies ...
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bioRxiv - Biochemistry 2024Quote: Wild-type BbZIP and the H149A/H151A variant were purified as described above and the N-terminal His6-tag was removed by incubation with thrombin overnight at 4°C before it was loaded to a Superdex Increase 200 column (GE Healthcare) equilibrated with a Cd-free solution containing 10 mM Hepes (pH 7.3) ...
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bioRxiv - Developmental Biology 2024Quote: ... Normalized protein samples were resolved on 4-20% tris-glycine gels and transferred to Amersham Hybond P 0.45 PVDF blotting membranes (GE Healthcare, 10600023). Membranes were blocked for 1h at room temperature in PBS-T with 5% milk ...
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bioRxiv - Plant Biology 2020Quote: ... the supernatant was collected and incubated for 5 min on 1 mL Ni Sepharose® 6Fast Flow (GE Healthcare) equilibrated with 10 mL binding buffer ...
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bioRxiv - Microbiology 2020Quote: ... coli BL21(DE3) and were isolated under native purification conditions using a 5 HisTrap High Performance Column (GE Healthcare) with imidazole gradient (10-250 mM ...
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bioRxiv - Biophysics 2021Quote: ... 10 μM ZnCl2 and 1 mM DTT) and loaded into a HiTrap Heparin HP 5 ml column (GE Healthcare) equilibrated with 6.25% of buffer HepB (buffer HepA supplemented with 2 M NaCl) ...
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bioRxiv - Neuroscience 2021Quote: ... filtered through 0.45 μm cut-off filters and loaded onto a HiTrap SP HP 5 ml column (GE Healthcare) for cation exchange ...
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bioRxiv - Cell Biology 2020Quote: ... Antibody dilutions in 5% milk/PBST: anti-MBP HRP conjugated, 1:50,000 (NEB, and anti-GST HRP conjugates, 1:2,000 (GE Healthcare,). Scanned western blot films were quantified using the gel analysis tool in ImageJ.
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bioRxiv - Molecular Biology 2021Quote: ... The protein fraction eluted with 250 mM imidazole was purified further by MonoQ 5/50 GL column (GE Healthcare) at pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... Filtered supernatant was loaded into a 5 mL Histrap column (Cytiva) connected to an FPLC AKTAxpress system (GE Healthcare). After affinity binding ...
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bioRxiv - Neuroscience 2021Quote: ... They were maintained within 37°C incubators at 5% CO2 in DMEM + high glucose (GE Healthcare Bio-Science, SH30022FS) supplemented with 9.09% fetal bovine serum (50 mL added to 500 mL DMEM ...
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bioRxiv - Molecular Biology 2020Quote: ... Sucrose gradient fractions containing ODA complexes were further purified over a MonoQ 5/50 anion exchange column (GE Healthcare) to separate out other axonemal dynein species ...
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bioRxiv - Biochemistry 2020Quote: ... purification of the protein was performed with a histidine affinity column (HisTrap FF crude 5 ml, GE Healthcare Amersham) taking advantage of the natural His present in the octapeptide repeat region of PrP ...
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bioRxiv - Immunology 2020Quote: ... Dilution series of TBK1 or IRF-3 (1.25, 2.5, 5, 10, 20 μM) in 1× HBS-EP+ buffer (GE Healthcare) were injected over the sensor chip at a flow rate of 30 μL/min ...