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Citations for GE Life Sciences :
1601 - 1650 of 3622 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was loaded onto a 5 ml HisTrap column (GE Healthcare), which was preequilibrated with the Lysis buffer plus 20 mM imidazole ...
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bioRxiv - Biophysics 2022Quote: ... which was used for affinity purification with 5 mL NiNTA column (GE Healthcare), which was previously equilibrated in NiA buffer (50 mM Tris pH8 ...
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bioRxiv - Neuroscience 2022Quote: ... After the reaction mixture had passed through a Nap-5 column (GE Healthcare) to remove excess dithiothreitol and iodoacetamide ...
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bioRxiv - Microbiology 2022Quote: ... were loaded onto a 5-ml Ni-affinity column (HisTrap HP, GE healthcare) and washed with buffer B (50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... and applied on a 5 mL HisTrap FF column (GE Healthcare Life Sciences) to remove residual His-tagged protein ...
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bioRxiv - Microbiology 2022Quote: ... 5 μL of alkylation solution [100 mM iodoacetamide (GE Healthcare, Chicago, IL, USA)] was added and incubated protected from light for 15 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... prior to loading onto a 5 mL HiTrap Q IEX column (GE Healthcare) equilibrated with 20 mM HEPES pH 7.5 followed by a wash step with the same buffer until a conductivity of 5 mS/cm was reached ...
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bioRxiv - Molecular Biology 2023Quote: ... the eluate was applied to a MonoQ 5/50 GL column (GE Healthcare) equilibrated in 25 mM Tris-HCl pH 7.2 ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was applied to a 5 ml His-TRAP column (GE Healthcare) and eluted with a gradient of 20 mM to 500 mM imidazole ...
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bioRxiv - Biophysics 2023Quote: ... supernatant was loaded onto a pre-packed 5 ml HisTrap column (GE Healthcare), equilibrated according to the supplier’s protocols with wash buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... the sample was passed through a 5 mL MBPTrap HP column (GE Healthcare) to remove the cleaved 6xHis-MBP tag ...
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bioRxiv - Biochemistry 2023Quote: ... and the filtrate loaded onto a 5 mL HisTrap HP column (GE Healthcare). The column was washed with 10 CV of Nickel Wash Buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... The filtrate was loaded onto a 5 mL HisTrap HP column (GE Healthcare), washed with 12 column volumes (CV ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were then scanned using an Amersham Typhoon 5 imager (GE Healthcare) with a 685 nm laser (IRshort720BP20 filter ...
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bioRxiv - Microbiology 2023Quote: ... The lysates were loaded into a HisTrap HP 5 mL column (GE Healthcare) previously equilibrated with binding buffer (50 mM Tris ...
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bioRxiv - Synthetic Biology 2024Quote: ... and purified to homogeneity with a 5 mL HiTrap HP column (GE healthcare) attached to a ÄKTA pure purification system using a linear gradient of 0-250 mM imidazole in buffer A over 35 CV ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was loaded onto two 5 mL HisTrap HP columns (GE Healthcare). After the columns were washed with six column volumes (CVs ...
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bioRxiv - Biophysics 2024Quote: ... the supernatant was purified by IMAC (5 mL HiTrap Chelating HP, GE Healthcare) using an FPLC system (ÄKTAprime ...
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bioRxiv - Biochemistry 2024Quote: ... IAC was performed on HiTrap NHS-activated HP 5-mL columns (GE Healthcare) coupled with 2 to 3 mg/mL of either MNB4 (an anti-PTX3 monoclonal antibody recognising an epitope in the N-terminal domain that was used to purify PTX3 ...
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bioRxiv - Synthetic Biology 2024Quote: ... and clarified lysates were applied to a 5 mL HisTrap column (GE Healthcare) that was equilibrated with Buffer A (20 mM Tris-HCl pH 8 ...
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bioRxiv - Biochemistry 2024Quote: ... the mixture was applied to a 5 mL HisTrap HP column (GE Healthcare) at a flow rate of 2.5 mL min-1 ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was loaded onto a 5 mL Ni-NTA column (GE Healthcare) connected to an ÄKTA Prime plus for purification.
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bioRxiv - Microbiology 2020Quote: ... 1 mM TCEP] by size-exclusion chromatography using a HiLoad 16/60 Superdex 200 gel-filtration column (GE Healthcare). PelXC232S eluted as a single Gaussian shaped peak ...
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bioRxiv - Cell Biology 2020Quote: ... In a 48 well plate, 25-32.5μL siRNA (for sequences, see table 1) was mixed with 25uL 1x siRNA buffer (GE Healthcare) containing 0.5uL Dharmafect 1 transfection reagent ...
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bioRxiv - Developmental Biology 2021Quote: ... For monoclonal antibody production, the DNA encoding mouse KCTD19 (residue 654-793 aa, NP_808459.1) was inserted into pGEX6p-1 (GE healthcare), and the expression vector was transformed into E ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM DTT) and fractions contacting samples were loaded onto size-exclusion column (Superdex 75 16/60, GE Healthcare) pre-equilibrated with 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Genetics 2020Quote: ... Three washes were performed with TBST followed by 1 h incubation with secondary antibody (ECL, HRP linked, GE Healthcare) and another series of washes ...
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bioRxiv - Genetics 2020Quote: ... Eluted fractions were diluted 6.5-fold with R buffer and loaded onto a 1 mL ResourceQ column (GE Healthcare). Rrp1 eluted at around 300 mM NaCl with a linear gradient of 0.1-1.0 M NaCl in R buffer ...
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bioRxiv - Neuroscience 2020Quote: ... before they were incubated with HRP-conjugated secondary antibodies (1:10000, NXA931 and NA934, GE Healthcare Europe, Freiburg, DE) also diluted in blocking solution for 1 h at RT ...
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bioRxiv - Synthetic Biology 2021Quote: ... Digested DNA was electrophoresed in 1% agarose and transferred by capillarity onto a nylon membrane (Hybond N+, GE healthcare). Membranes were hybridized in Church buffer at 68°C with two different 32P-radiolabeled probes (Church and Gilbert ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoreactivity was detected using species-appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies (1:5000; GE Healthcare, Cleveland, OH, USA). Western blots were imaged via HRP chemiluminescent detection (Azure Biosystems ...
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bioRxiv - Biochemistry 2021Quote: ... The lysates were centrifuged and the supernatants used for the purification on HisTrap HP columns (1 mL; GE Healthcare) connected to an AKTA Explorer system (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... to <1 mL and injected onto a Superdex™ 200 10/300 GL Increase size-exclusion column (GE Healthcare) equilibrated in gel filtration buffer ...
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bioRxiv - Biochemistry 2020Quote: ... to <1 mL and injected onto a Superdex™ 200 10/300 GL Increase size-exclusion column (GE Healthcare) equilibrated in gel filtration buffer (20 mM Tris pH 7.2 ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... An enhanced chemiluminescence (ECL)-horse radish peroxidase (HRP) conjugated secondary antibody (NA934V, 1:1000 dilution, GE Healthcare, Uppsala, Sweden) and SuperSignal West Femto Chemiluminescent Substrate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Secondary detection for western blotting used anti-rabbit HRP antibody diluted 1:10,000 (Amersham ECL, GE Healthcare, Chicago, IL). Polyclonal affinity purified NS3 and NS5 proteins were produced as previously described (20) ...
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bioRxiv - Immunology 2021Quote: ... IgGs were purified from ~1 ml of plasma with equal volume of settled Protein A Sepharose resin (GE Healthcare). Samples were eluted from the resin with 0.1 M glycine at pH 2.5 and immediately neutralized with 1 M Tris-HCl pH 8 ...
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bioRxiv - Plant Biology 2021Quote: ... The recombinant proteins were then purified using a 1 ml HisTrap HP column and ÄKTA™ system (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and the resulting soluble fraction was applied on a Ni-NTA column (GE Healthcare, His Trap FF 1 ml) and purified according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... MERS-CoV NTD and SARS-CoV-1 NTD were then further purified using a Superdex 75 column (GE Healthcare) in 2 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... Chromosomal DNA fragments were separated on a 1% agarose gel and blotted onto Hybond N+ nylon membranes (GE Healthcare), following standard techniques (Sambrook and Russell ...
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bioRxiv - Microbiology 2020Quote: ... The His-tagged GlpQ protein was purified by Ni2+-affinity chromatography using a 1 ml HisTrap column (GE Healthcare) followed by size exclusion chromatography on a HiLoad 16/60 Superdex 200 pg column (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Human mAbs were purified by loading the supernatant onto a 1 mL Protein A HP HiTrap column (GE Healthcare). Columns were equilibrated and washed using Dulbecco’s phosphate buffered saline (DPBS) ...
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bioRxiv - Biophysics 2021Quote: ... and MtCNNMCorC (residues 333-417) were subcloned into BamHI and XhoI sites of pGEX-6P-1 vector (GE Healthcare) with an N-terminal GST-tag.
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bioRxiv - Biophysics 2020Quote: ... 50 mM KAc,1 mM DTT) Further purification was performed using size exclusion chromatography (Superose-6 increase, GE Healthcare) using Shimadzu High Performance Liquid Chromatograph ...
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bioRxiv - Biophysics 2020Quote: ... Protein fractions (eluted at ~ 1 M NaCl) were then purified using a HiLoad 16/60 Superdex 75 (GE Healthcare) column (SEC ...
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bioRxiv - Biochemistry 2022Quote: ... 100 µL TIR-SAVED (10.9 mg.mL-1) were injected into the Superose 12 column 10/300 GL (GE Healthcare) in 20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 mM DTT] and filtered on a Superdex 200 10/300 GL column (GE Healthcare, Little Chalfont, UK). Aliquots of the protein were flash-frozen in liquid nitrogen and stored at -80 °C.
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bioRxiv - Neuroscience 2021Quote: ... Immunoreactivity was detected using a horseradish peroxidase (HRP)-conjugated anti-rabbit immunoglobulin G (IgG, 1:10,000, NA934 GE Healthcare). Tau13 and Tau5 immunoreactivity were detected using an anti-mouse IgG (1:10,000 ...
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bioRxiv - Microbiology 2020Quote: ... Ten μl of each culture were spotted onto 15 mm grade 1 Whatman filter discs (GE Healthcare Life Sciences). After drying for 1 minute at room temperature ...