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Citations for GE Life Sciences :
1851 - 1900 of 3622 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The membrane was washed in 0.1% SDS/0.1X SSC and hybridized DNA was visualized and quantitated by phosphor-imaging on a Typhoon Scanner (GE Healthcare). The 32P-radiolabeled probe was generated from a plasmid containing the entire HPV16 genome by radiolabeling using a Random Prime labeling kit (Roche).
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bioRxiv - Microbiology 2020Quote: ... non-fat dry milk in TBST for 1 hr at room temperature: anti-rabbit or anti-mouse IgG-HRP conjugated antibody from sheep (both 1:10,000 GE Healthcare). Proteins were visualized using ECL or SuperSignal West Femto chemiluminescence reagents (Pierce ...
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bioRxiv - Biophysics 2020Quote: ... which is complementary to the tracrRNA 5’-end was labeled with ATTO647N-NHS (1:60 DNA:dye molar ratio) and purified using G-25 micro spin column (Illustra, GE Healthcare). The measured labeling efficiency of ATTO647N-oligonucleotide was >80% ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was loaded onto a 1-ml HisTrap column connected to an ÄKTA Explorer 100 purification system (GE Healthcare), previously equilibrated with Buffer A ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting eluate was manually applied at room temperature to a 1 mL HiTrapQ anion exchange column (GE Healthcare # 29051325) that had been pre-equilibrated in buffer B ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 hour at 30V onto Amersham Hybond P 0.45 PVDF membranes (GE Life Sciences, Pittsburgh, PA; cat # GE 10600029). After transfer membranes were rinsed in TBST and blocked for one hour in either 5% milk (PERK ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were washed with TBST three times and incubated with sheep anti-mouse or donkey anti-rabbit IgG conjugated with horseradish peroxidase (HRP) (1:4,000, GE Healthcare) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was incubated at 4°C for 1 h with Ni-charged IMAC Sepharose 6 Fast Flow (GE Healthcare). The resin was then washed three times with His-A buffer (lysis buffer + 20 mM imidazole) ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.1% FA (Fluka) and crosslinked peptides were enriched by separation on a Superdex Peptide PC 3.2/30 column (GE Healthcare), using an Agilent 1100 chromatography system at 95 μL/minute with fraction collection ...
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bioRxiv - Cell Biology 2021Quote: ... The reaction was quenched with 1 μl 14.3 M βME followed by final buffer exchange using size exclusion chromatography (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Plant Biology 2020Quote: ... The supernatant was filtered through tandem 1-μm and 0.45-μm filters before Ni2+-affinity purification (HisTrap excel, GE Healthcare). Ni2+-bound proteins were washed with buffer A and eluted directly onto a coupled Strep-Tactin Superflow XT column (IBA ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated with the secondary antibody (HRP-conjugated anti-mouse or anti-rabbit IgG antibody, GE Healthcare; 1:2000) in TBST plus 2% BSA ...
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bioRxiv - Plant Biology 2021Quote: ... in running buffer (20 mM Tris, pH 8, 150 mM NaCl, 1 mM DTT) supplemented with 0.05% of P20 surfactant (GE Healthcare). Anti-MBP murin monoclonal antibody (Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... and eluted in superdex buffer (20 mM Tris [pH 8.0], 300 mM NaCl, 1 mM TCEP) using the AKTA Explorer FPLC system (GE Healthcare). The protein was concentrated to 7.5 mg/ml.
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bioRxiv - Plant Biology 2021Quote: ... α-P4 or α-P6 before subsequent addition of secondary horseradish peroxidase (HPR)-conjugated antibodies (1:20.000; Amersham, GE Healthcare). The immunoreaction was developed using the ECL Prime kit (Amersham ...
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bioRxiv - Microbiology 2021Quote: ... the obtained supernatants were filtered through sterile PVDF filters (pore size 0.22 μm) and loaded on 1 ml His-Trap columns (GE Healthcare), pre-equilibrated with ten column volumes of each Millipore water and sodium phosphate buffer A ...
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bioRxiv - Microbiology 2020Quote: ... The clarified lysate was then passed using an ÄKTA Purifier FPLC through a 1 ml HisTrap HP column (GE Healthcare), pre-equilibrated in lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... The detected proteins were revealed with 1:3,000-diluted horseradish peroxidase (HRP)-conjugated anti-mouse IgG secondary antibody (GE Healthcare) using a Pierce ECL chemiluminescence substrate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... The detected proteins were revealed with 1:3,000-diluted horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse IgG secondary antibodies (GE Healthcare) using a Pierce ECL chemiluminescence substrate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... with a JA30.50 Ti rotor at 20,000 ×g and applied to a HiTrap SP HP column (GE Healthcare; 1 ml) equilibrated in buffer A (20 mM Na-phosphate buffer (pH 7.0) ...
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bioRxiv - Cell Biology 2020Quote: ... The protein purification was done through affinity chromatography on a Ni-column (HiTrap chelating HP column 1 ml, GE HealthCare). The column was loaded with a filtered solution of 100 mM nickel chloride ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was centrifuged at 18500 g for 30 minutes and the supernatant filtered through a 0.22 µm PES filter and loaded onto a 1 mL Ni column (GE healthcare) in buffer L with 10 mM immidazole ...
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bioRxiv - Microbiology 2022Quote: ... the concentration of cAMP in lysates from infected THP-1 cells was determined using the cAMP enzyme immunoassay (GE Healthcare) as previously described (57) ...
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Proteolytic cleavage of the extracellular domain affects signaling of parathyroid hormone receptor 1bioRxiv - Pharmacology and Toxicology 2022Quote: ... followed by horseradish peroxidase-conjugated goat anti-rabbit (1:10,000) and detection on Super RX X-ray film (Fujifilm) using ECL Plus reagent (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... 1 mL of the resulting supernatant was incubated for 1 hour at 4°C on constant agitation of 1,000 rpm with 100 µL glutathione sepharose (GS) resin (GE Healthcare), pre-equilibrated in buffer F ...
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bioRxiv - Cell Biology 2022Quote: ... The lysate was centrifuged at 18,500 × g for 30 min and the supernatant filtered through a 0.22 µm PES filter and loaded onto a 1 mL Ni column (GE healthcare) in buffer L with 10 mM imidazole ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant after centrifugation at 17,000 g at 4 °C for 1 hr was incubated with Glutathione Sepharose 4B (GE healthcare) at 4 °C for 3 hrs ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was incubated with either 1 ml of Amersham ECL substrate western blotting detection reagent (GE Healthcare lot# 9622301) or for higher sensitivity ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were then washed three times for five minutes with 0.1% PBS-Tween20 and incubated for 1 hour at room temperature with anti-rabbit HRP secondary antibody (GE healthcare) in blocking solution ...
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bioRxiv - Molecular Biology 2020Quote: ... They were then incubated for an hour at RT with 1:5,000 anti-rat horseradish peroxidase conjugated antibody (GE Healthcare). Protein bands were detected on X-ray film using SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... and the supernatant injected (using 1 ml injection loop) in a Superdex 75 10/300GL gel filtration column (GE Healthcare), previously equilibrated with 50mM Tris pH8 buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were incubated with the appropriate horseradish peroxidase (HRP)-labeled secondary antibody (1:5000 dilution; GE Healthcare UK Ltd, UK). Protein bands were detected using enhanced chemiluminescence (Luminata Crescendo ...
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bioRxiv - Cell Biology 2020Quote: ... and within 1 h after taking out of the incubator on a commercial structured illumination microscope (DeltaVision|OMXv4.0 BLAZE, GE Healthcare). All the images were acquired using 488 nm laser with power set to either 10% or 31.5% ...
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bioRxiv - Neuroscience 2021Quote: ... free-floating sections were washed in PBS and incubated overnight in Cy3-conjugated streptavidin (1:1000 dilution; PA43001, GE Healthcare) in “Triton-PBS” (PBS containing 0.3% v/v Triton X-100 [Sigma]) ...
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bioRxiv - Biochemistry 2021Quote: ... the purified OxlT was mixed with purified D5901-A08-Fab at a 1:1.3 molar ratio at 4 °C overnight and applied to a HiLoad 16/60 Superdex200 pg column (GE healthcare) using buffer D (20 mM MES-KOH ...
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bioRxiv - Biochemistry 2021Quote: PeSkC and RoSBC samples were applied to a 1.8×13cm Sephadex G100 column at a flow rate of 1ml min-1 using an ÄKTAprime plus unit (GE Healthcare). Void volume was calculated by running blue dextran ...
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bioRxiv - Molecular Biology 2022Quote: ... were used at 1:10,000 and blots developed with ChemiGlow detection reagent (ProteinSimple) and visualized using ImageQuant LAS 4000 chemiluminescent camera (GE Healthcare). For RNA-Immunoprecipitations FLAG-Immunoprecipitation from DHX34-GFP-FLAG A5 cells were performed with in NET2 buffer (50 mM Tris-HCl pH7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The suspension was then centrifuged for 1 h at 16,000 g at 4 °C and the supernatant was added to 1 mL of glutathione beads (GE Healthcare) equilibrated in PBS ...
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bioRxiv - Biochemistry 2020Quote: Solid-phase synthesis of oligoribonucleotide was performed on a 1 μmol scale using AKTA Oligopilot plus 10 synthesizer (GE Healthcare) and Custom PrimerSupport™ Ribo A 40 (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were further incubated with horseradish peroxidase (HRP)-conjugated anti-rabbit IgG secondary antibody (1:3,000; GE Healthcare, USA) in PBST-BSA for 1 h ...
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bioRxiv - Biochemistry 2020Quote: ... After incubation for 1 hour at room temperature proteins were purified using PD MiniTrap™ G-25 columns (GE Healthcare).
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bioRxiv - Biophysics 2021Quote: ... the myosin was purified by His-tag affinity purification with a 1 ml HisTrap HP nickel-sepharose column (GE Healthcare). The eluted myosin solution was then dialyzed overnight at 4 °C in a low-salt buffer (25 mM imidazole pH 7.0 ...
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bioRxiv - Microbiology 2020Quote: ... cells were lysed using M110-L microfluidizer (Microfluidics) and the protein was isolated using 1-mL HisTrap column (GE Healthcare). Protein was concentrated with Amicon Ultracel-10K (Millipore ...
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bioRxiv - Microbiology 2020Quote: ... Alba proteins were run on an ion-exchange column (HiTrap SP column, 1 ml, GE Healthcare UK Ltd. Buckinghamshire, England) following the manufacturer’s protocol and as described previously (Maruyama et al. ...
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bioRxiv - Microbiology 2020Quote: ... equilibrated with lysis buffer at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare). After washing with 20 ml lysis buffer ...
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bioRxiv - Immunology 2020Quote: ... for 1 h at 4 °C in an overhead tumbler before retrieving immune complexes with protein G Sepharose (GE Healthcare). Beads were washed and complexes were treated with Endoglycosidase H (New England Biolabs ...
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bioRxiv - Synthetic Biology 2020Quote: ... The extracellular media was then diluted two-fold with 20 mM HEPES buffer at pH 7.0 and applied to 1 column volume (CV) of DEAE Sepharose Fast Flow Resin (from GE Healthcare) equilibrated with 10 CV of 20 mM HEPES buffer at pH 7.0 (1 CV equaled 2 ml of settled resin for 25 ml cultures and 1 CV equaled 4 ml of settled resin for 250 ml cultures) ...
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bioRxiv - Biophysics 2020Quote: The protein extract was loaded onto a 1 mL HiTRAP Heparin column HP (GE Healthcare Life Sciences, 17-0406-01) equilibrated with Buffer A (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... 20 mL of the clarified lysate was loaded onto a 1 mL HiTrap IMAC Sepharose FF column (GE Healthcare, Illinois) charged with Ni2+ and preequilibrated with buffer A ...
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bioRxiv - Bioengineering 2020Quote: ... and supernatant was filtered with a 0.22 μm filter before loading onto a 1 ml HisTrap HP column (GE Healthcare). Proteins bound to the column were washed with 10 column volumes of washing buffer (50 mM Tris ...