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Citations for GE Life Sciences :
1501 - 1550 of 4331 citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Dialysed protein was passed through a 5 mL MBPTrap column (GE Healthcare). The flow-through fraction was concentrated and further purified by size exclusion chromatography using a Superdex 200 (16/600 ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were electrotransferred onto a PVDF membrane (AmershamTM HybondTM, GE Healthcare) in 25 mM Tris ...
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bioRxiv - Neuroscience 2021Quote: ... For subsequent FPLC purification (Fast Protein Liquid Chromatography; ÄKTAprime plus, GE Healthcare) all solutions were degassed by vacuum-driven filtration (Stericup 500 mL Durapore 0.45 μm PVDF ...
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bioRxiv - Physiology 2021Quote: ... Proteins were visualized after transfer onto a PVDF blotting membrane (GE Healthcare) and incubation with specific primary antibodies using horseradish peroxidase-conjugated secondary antibodies ...
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bioRxiv - Microbiology 2021Quote: ... Protein G Sepharose 4 Fast flow beads (GE Healthcare, GE17-0618-01) were incubated with α-DAF (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... Proteins of interest were purified with an ÄKTA avant system (GE Healthcare) using strep-tag affinity chromatography (Wash buffer ...
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bioRxiv - Microbiology 2020Quote: Proteins were eluted directly into a 1 mL HisTrap column (GE Healthcare) using purification buffer supplemented with Desthiobiotin 2.5 mM (IBA Solutions For Life Science™) ...
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bioRxiv - Immunology 2021Quote: ... Protein bands were detected with Amersham Prima Western Blotting (RPN22361, GE Healthcare) on the ChemiDoc Gel Imaging System (Bio-Rad Laboratories) ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein was purified from the medium by Ni Sepharose excel (GE Healthcare) affinity chromatography ...
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bioRxiv - Immunology 2020Quote: ... antibodies were immobilized on Series S Protein G sensor chip (GE Healthcare) at a density of 2000 RU ...
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bioRxiv - Immunology 2020Quote: ... Concentrated proteins were further purified with a Superdex 200 increase (GE Healthcare) column equilibrated with 50 mM Tris ...
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bioRxiv - Immunology 2021Quote: ... Which were then purified using HiTrap Protein-A column (GE healthcare, UK). The SARS-CoV-2 spike (S ...
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bioRxiv - Immunology 2020Quote: ... GST-fusion proteins were purified using glutathione-Sepharose 4B columns (GE Healthcare). Protein-bound glutathione beads were thoroughly washed with cold PBS six times ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were detected with HRP-conjugated secondary antibodies (1:10,000; GE Healthcare) and visualized using an AI600 Imager (GE Healthcare) ...
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bioRxiv - Immunology 2021Quote: ... The protein was further purified with Sephacryl S-300 HR (GE Healthcare) with PBS ...
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bioRxiv - Immunology 2021Quote: ... and the protein was purified using Ni-NTA agarose beads (GE Healthcare). The protein was further purified on a Superdex 200 Increase 10/300 GL size exclusion column (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... Transfer of protein bands onto a nitrocellulose membrane (GE Healthcare, Amersham, UK) was performed at 15 V overnight/ 300mA for 3-5 h or semi-dry at 90V for 1.5 h ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were further purified by size-exclusion chromatography (SD200, GE Healthcare) in buffer B and either used for biochemical assays or SAXS experiments ...
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bioRxiv - Cell Biology 2021Quote: ... The protein was further purified by gel filtration (Superdex-200, GE Healthcare) in a column equilibrated with storage buffer (50 mM Tris-HCl 7.4 ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration was estimated using Quant kit (GE Healthcare, Chicago, Il, USA). Ten-microgram aliquots of proteins were diluted with 15 µl of 50 mM NH4HCO3 and reduced with 5.6 mM DTT for 5 min at 95°C ...
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bioRxiv - Cell Biology 2020Quote: ... The extracts were then incubated with protein A-Sepharose beads (GE Healthcare) for 3 h at 4°C and washed five times with EB containing 1% Tween 20 ...
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bioRxiv - Pathology 2023Quote: ... Proteins were blotted onto nitrocellulose membranes (Amersham, GE Healthcare, Chicago, IL, USA). Western blotting was performed with the use of primary antibodies listed in Table 2 ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were further purified using a 5mL HiTrap Q FF (GE Healthcare) on an AKTA pure ...
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bioRxiv - Biophysics 2023Quote: ... The cleaved protein was loaded onto a HiTrap SP column (GE Healthcare) and eluted over a linear KCl (0.25–1 M ...
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bioRxiv - Immunology 2023Quote: ... Polyclonal IgG was purified from plasma using Protein A resin (GE Healthcare) and digested into Fabs using immobilized papain resin as described previously46 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The purified protein was buffer exchanged with PD-10 columns (GE Healthcare) into 100 mM potassium phosphate buffer pH 7.6 ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were purified by Ni2+-Sepharose 6 Fast Flow (GE healthcare) affinity chromatography with the washing buffer (50mM Tris-HCl ...
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bioRxiv - Cancer Biology 2023Quote: ... The immune complex was incubated with protein A-Sepharose beads (GE Healthcare) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... and protein was purified by cation exchange chromatography (HiTrap SP, GE Healthcare), followed by size exclusion chromatography (HiLoad 16/600 Superdex 200 pg ...
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bioRxiv - Systems Biology 2023Quote: ... The proteins were quantified using ImageQuant TL software (GE Healthcare, version 7.0). For further detection ...
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bioRxiv - Cell Biology 2023Quote: ... we transferred the proteins onto nitrocellulose membranes (GE Healthcare, Little Chalfont, UK) using the wet transfer method for 1 hour at 250 mA (4 °C) ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were further purified by gel filtration chromatography (Superdex-200, GE Healthcare) with columns equilibrated in storage buffer (50 mM Tris-HCl 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were further purified by gel filtration chromatography (Superdex-200, GE Healthcare) with columns equilibrated in storage buffer (50 mM Tris-HCl 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were immunoblotted on 0.2 µm nitrocellulose membranes (GE Healthcare Amersham, 10600006) using Pierce Methanol-free Transfer buffer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Inactivated serum was incubated for 20 hrs with protein G (GE Healthcare) or CaptureSelect resin (Thermo Fisher ...
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bioRxiv - Physiology 2022Quote: ... Resolved proteins were electrotransferred onto polyvinylidene difluoride (PVDF) membrane (GE Healthcare, USA) at 100V ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting protein samples were further purified by SP-Sepharose (GE Healthcare) and Superdex200 (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... before being purified with protein A agarose (GE Healthcare, 17-1279-01) followed by IP ...
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bioRxiv - Biochemistry 2022Quote: ... and then bound to a HiTrap Protein G HP column (GE Healthcare). After washing ...
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bioRxiv - Developmental Biology 2022Quote: ... GST fusion proteins were affinity purified over Gluthione Sepharose 4B (GE Healthcare). After extensive washing ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were then precipitated using the 2D Clean Up Kit (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Separated proteins were transferred to a nitrocellulose membrane (GE Healthcare, Freiburg, Germany) in transfer buffer (25 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was applied onto a carboxymethyl sepharose fast flow column (GE Healthcare) and eluted with a sodium chloride gradient that contained 50 mM MES pH 6.5.
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bioRxiv - Neuroscience 2023Quote: ... followed by protein G Sepharose beads (GE healthcare life science, Cat #17061801) for 2 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were purified using an Akta Purifier 10 chromatograph (GE Healthcare) and His Trap column ...
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bioRxiv - Physiology 2024Quote: ... Proteins were visualized after transfer onto a PVDF blotting membrane (GE Healthcare) and incubation with specific primary antibodies using horseradish peroxidase-conjugated secondary antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein were incubated with Streptavidin beads (GE Healthcare, USA) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Bound proteins were then purified via binding to glutathione Sepharose (GE Healthcare) and washed ...
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bioRxiv - Developmental Biology 2023Quote: ... all proteins were desalted using PD MiniTrap G-25 columns (GE Healthcare) and protein quality was checked by CBB staining and immunoblotting ...
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bioRxiv - Plant Biology 2023Quote: ... followed by the protein transfer to a Protran nitrocellulose membrane (GE Healthcare). To control for equal loading of whole protein ...