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Citations for GE Life Sciences :
1501 - 1550 of 3511 citations for 7 Butyl 8 mercapto 1 3 dimethyl 3 7 dihydro purine 2 6 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... the sample was treated with 100 mM phosphate buffer pH 7.5 and 50 mM EDTA before purification by gel filtration at room temperature on a Superose-6 Increase column (GE Healthcare) equilibrated with buffer containing 25 mM TRIS pH 8 ...
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bioRxiv - Biochemistry 2023Quote: ... dIgA-604 and SIgA-604 were purified using Ni-NTA beads (Smart-Lifesciences) and a Superose 6 Increase column (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... The harvested cells were lysed by pulsed sonication and the soluble fraction was affinity purified using Ni2+-Sepharose (Ni Sepharose 6 Fast Flow, GE Healthcare). The resulting MsLadC variants ...
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bioRxiv - Biophysics 2023Quote: ... The 6×His-tagged SUMO fusion proteins were purified using a HisTrap™ HP Ni2+ affinity column (GE Healthcare Life Sciences). The fractions containing fusion protein were pooled and exchanged into a SUMO protease cleavage buffer (20 mM Tris-HCl at pH 8.0 ...
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bioRxiv - Immunology 2023Quote: ... Affinity-purified proteins were incubated with HRV-3C protease to remove purification tags and subsequently purified by size-exclusion-chromatography on a Superose 6 Increase column (GE Healthcare) in TBS
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was digested with the TEV protease overnight and further purified by gel filtration chromatography using a Superose 6 column (GE Healthcare), eluted in buffer C (25 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... A 2-fold molar excess of LF was mixed with 925 pmoles of hexon and incubated on ice for 5 min followed by SEC on Superose 6 increase 10/300 (GE Healthcare) equilibrated with buffer D ...
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bioRxiv - Biochemistry 2024Quote: ... The N-terminal GFP-His10 tag was cleaved overnight with 3C protease before gel filtration chromatography by using a Sepharose 6 Increase 10/300 GL column (GE Healthcare). For the purification of apo proteins ...
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bioRxiv - Biochemistry 2024Quote: ... Bio-Beads were removed by centrifugation (250g, 10 min) and the supernatant was subjected to size-exclusion chromatography in a Superose 6 increase column (GE healthcare) equilibrated in SEC buffer (50 mM Tris-HCl ...
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bioRxiv - Immunology 2023Quote: ... The S proteins were then separated by chromatography using resin attached with streptavidin and further purified by size exclusion chromatography using a Superose 6 10/300(GE Healthcare) in 20 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... Then the complex was concentrated to a volume of ∼100 µL and loaded onto a Superose 6 5/150 GL size-exclusion column (GE Healthcare). Peak fractions were pooled and concentrated to ∼2 µM for cryo-EM sample preparation.
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bioRxiv - Neuroscience 2024Quote: Molecular masses of purified gephyrin and variants were detected by analytical size exclusion chromatography (SEC) using a Superose 6 increase 5/10 (GE Healthcare). Typically ...
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bioRxiv - Microbiology 2024Quote: ... and ClpCDWB (purified as described in Supplemental Materials and Methods) were performed on a Superose 6 10/300 Increase column (GE Healthcare) at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The urea concentration was set to 6 M and the sample was applied to a 5 mL HiTrap QFF anion exchange column (GE healthcare) and was eluted using a 30 CV long gradient from low salt buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Pathology 2024Quote: ... 50 µL of sample were injected on a HPLC system (Alliance, Waters) and separated on a Superose 6 increase 10/300 GL column (GE Healthcare) equilibrated with 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... buffer exchanged sample was lastly purified by size exclusion chromatography (SEC) at 4 °C by injecting sample onto a Superose 6 Increase 10/ 300 GL column (GE Healthcare) equilibrated in SEC buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... A total reaction mixture of 500 µL was used for downstream size exclusion purification utilizing a Superose 6 10/300 column (GE Healthcare) to remove excess PKA and phosphorylation reagents ...
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bioRxiv - Biochemistry 2024Quote: ... The splicing product was concentrated to a volume of ∼100 µL and loaded onto a Superose 6 5/150 GL size-exclusion column (GE Healthcare) which is pre-equilibrated with complex buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... Then the complex was concentrated to a volume of ∼100 µL and loaded onto a Superose 6 5/150 GL size-exclusion column (GE Healthcare). Peak fractions were pooled and concentrated to 2µM for cryo-EM sample preparation.
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bioRxiv - Biophysics 2024Quote: ... 300 mM imidazole and 0.6% C8E4 (tetraethylene glycol monooctyl ether) and concentrated before loading onto a Superdex 200 increase 10/300 GL column (GE Healthcare) equilibrated with buffer G containing no imidazole ...
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bioRxiv - Biophysics 2024Quote: ... the remaining 10 ml of concentrated 6-His-AdhE in buffer A were loaded onto a HiLoad 26/600 Superdex 200 pg column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Biophysics 2024Quote: ... were used with flow rates at 6 ml/min and loaded either by hand or by using an AKTA Purifier (GE Healthcare) following manufacturers guidelines ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing desired protein were pooled and concentrated using a 100 kDa centrifugal concentrator (Amicon UFC810024) and loaded on gel filtration chromatography using a Superose 6 Increase 10/300 column (GE Healthcare) using the following buffer ...
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bioRxiv - Biochemistry 2024Quote: ... concentrated using a Vivaspin concentration with 100,000 MWCO and loaded onto a Superose 6 Increase 10/300 size exclusion chromatography column (GE Healthcare Lifescience) equilibrated with the same HEPES-dialysis buffer to remove thrombin ...
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bioRxiv - Microbiology 2020Quote: ... S protein was purified by running the supernatant through a streptactin column and then by size exclusion chromatography using a Superose 6 increase 10/300 column (GE Healthcare Biosciences). Protein fractions corresponding to the trimeric S protein were collected and concentrated.
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bioRxiv - Cell Biology 2020Quote: ... Elutions with CAV1 were concentrated and further purified by size exclusion chromatography using Superpose 6 Increase 10/300 GL (GE Healthcare, Illinois) equilibrated with a buffer composed of 200 mM NaCl ...
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bioRxiv - Immunology 2022Quote: ... BG505 Env trimer was purified from transfected cell supernatants as described47 by 2G12 immunoaffinity and size-exclusion chromatography (SEC) with a Superose 6 16/600 column followed by Superdex 200 Increase 10/300 GL column (GE Life Sciences).
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bioRxiv - Biochemistry 2019Quote: ... 100 μL of purified protein/complex at 2-5 mg/mL was injected onto a Superdex 200 Increase 10/300 GL or Superose 6 Increase 10/300 GL column (GE Life Sciences) in a buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: Size exclusion chromatography experiments with HeLa cell extracts were performed with a pre-packed Superose 6 10/300 GL column (GE Healthcare ©). The column was equilibrated with buffer A (20 mM Hepes ...
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bioRxiv - Microbiology 2019Quote: ... The concentrated protein complex was further purified using size-exclusion chromatography with a Superose-6 Increase 10/300-GL (GE Life Sciences) column equilibrated in Buffer B (50 mM HEPES ...
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bioRxiv - Molecular Biology 2019Quote: ... Affinity-purified proteins were subjected to size-exclusion chromatography on a Superdex 200 10/300 column or on a Superose 6 5/150 column (GE Healthcare, Germany) in gel filtration buffer (50 mM Hepes pH 8.0 ...
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bioRxiv - Genomics 2021Quote: ... Neisseria sicca and Neisseria elongata was migrated on a 6% polyacrylamide gel and then transferred onto nitrocellulose membrane (Amersham Hybond™ N+ from GE healthcare). The oligonucleotides were labeled in 5’ by using 5 pmoles of oligonucleotide ...
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bioRxiv - Cell Biology 2021Quote: ... The eluted Cavin4a-FL and Cavin4b-FL proteins were loaded on size exclusion chromatography column Superose™ 6 Increase 10/300 GL column (GE healthcare) pre-equilibrated with 0.22 μm filtered 50 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... The resulting pellet was resuspended in TET and complexes were further separated by Superose 6 10/300 column chromatography in TET buffer with 150 mM NaCl using an AKTA Pure system (GE Life Sciences). The sample collected from the column was used for microscopy and visualized by SDS-PAGE with silver staining (ProteoSilver™ Plus Silver Stain Kit) ...
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bioRxiv - Biochemistry 2021Quote: ... Cleaved topo VI fractions were pooled and concentrated using Amicon Ultra 15 mL centrifugal filter units (30 kDa cut off, MilliporeSigma) before being passed down a Superose 6 10/300 (GE Life Sciences) column in Buffer C (20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... The resulting pellet was resuspended in TET and complexes were further separated by Superose 6 10/300 column chromatography in TET buffer with 150 mM NaCl using an AKTA Pure system (GE Life Sciences). The sample collected from the column was used for microscopy ...
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bioRxiv - Biochemistry 2019Quote: ... the reaction was quenched with 10 mM DTT and subjected to size-exclusion chromatography on a Superose™ 6 Increase 10/300 (GE Healthcare) in GF buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... The reaction was stopped with 1.7 mM Tris-HCl pH 8.0 and crosslinked TcdA1-BSA-Lewis X was purified from excess of BSA-Lewis X by SEC using a Superose 6 3.2-300 column (GE Healthcare Life Sciences).
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bioRxiv - Genomics 2021Quote: ... Barcoded beads were resuspended in lysis buffer (200 mM Tris-HCl pH8.0, 20 mM EDTA, 6% Ficoll PM-400 (GE Healthcare/Fisher Scientific), 0.2% Sarkosyl (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2020Quote: ... The dried butanol fraction was then redissolved in 20mM of sodium phosphate buffer (pH 6) and desalted using a NAP10 desalting column prepacked with Sephadex G-25 resin (GE healthcare, USA). Finally ...
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bioRxiv - Microbiology 2020Quote: ... The protein (5-6 ml) was further purified by FPLC over a 26/60 Superdex 200 pg size exclusion column (GE Life Sciences) equilibrated with 20 mM Tris (pH 8.0 ...
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bioRxiv - Immunology 2020Quote: ... Complex samples were either diluted to 0.02 mg/mL and applied to glow discharged negative stain grids or they were SEC purified using a Superose™ 6 Increase 10/300 GL (GE Healthcare) column to remove excess Fab prior to EM grid preparation ...
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bioRxiv - Genetics 2020Quote: ... the eluted protein solution was concentrated 10 times in a Vivaspin concentrator 30 kDa cut off and loaded in a Superose 6 increase 10/300 (GE Life Sciences) with PBS as a carrier buffer ...
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bioRxiv - Biochemistry 2021Quote: ... filtered (0.22 mm) and then applied to 5 mL HiTrap Chelating HP columns (GE Healthcare, protein from 6 L cells per column) charged with Ni2+ and equilibrated with 4 column volumes of buffer C (20 mM Tris-HCl ...
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bioRxiv - Plant Biology 2021Quote: Secreted candidate proteins were purified by immobilized metal ion affinity (IMAC) using Ni Sepharose 6 Fast Flow (GE Healthcare, Chicago, IL, EUA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... The eluent was concentrated and further purified by using a size-exclusion chromatography (SEC) column (Superose 6 increase 10/300 GL, GE Healthcare, USA) in 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2021Quote: ... The samples (equivalent to ∼85 µg microsomes) were then analyzed with an HPLC system and injected to a Superose 6 column (GE Life Sciences), equilibrated in a running buffer containing 25 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2021Quote: The protein was purified from filtered cell supernatants using Strep-Tactin resin (IBA) before being subjected to additional purification by gel filtration chromatography using a Superose 6 10/300 column (GE Healthcare, USA) in 1 × PBS ...
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bioRxiv - Biophysics 2022Quote: ... Sample homogeneity was investigated by analytical size exclusion chromatography on a Superose® 6 10/300 GL gel filtration column (GE Healthcare). Aliquots of purified EFL at 0.4 mg/mL were flash frozen in liquid nitrogen and stored at −70 °C.
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bioRxiv - Microbiology 2022Quote: ... The leukocyte-depleted red blood cell pellet was then resuspended in 10 ml PBS and 5 ml were carefully layered on 6 ml of 70% (v/v) isotonic Percoll cushion (GE Healthcare, UK). After centrifugation at 1200 x g for 15 mins ...