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Citations for GE Life Sciences :
1451 - 1500 of 3511 citations for 7 Butyl 8 mercapto 1 3 dimethyl 3 7 dihydro purine 2 6 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Protein was purified from filtered cell culture supernatants by StrepTactin resin (IBA) and by size exclusion chromatography using Superose 6 column (GE Healthcare) in 10 mM Tris pH8,150 mM NaCl or 2 mM Tris pH 8 ...
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bioRxiv - Cell Biology 2021Quote: ... the protein was then mixed with equimolar KIFBP and subjected to size exclusion chromatography a second time on a Superose 6 column (GE Healthcare) equilibrated with gel filtration buffer ...
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bioRxiv - Biophysics 2019Quote: ... The protein solution was concentrated with a 100-kDa cut-off centricon (Milipore) and further purified by Superose-6 increase 10/300 column (GE Healthcare) using a buffer containing 50 mM Imidazole pH 6.0 ...
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bioRxiv - Microbiology 2022Quote: ... Antibody supernatant was harvested 6 days following transfection and purified using protein G affinity chromatography following the manufacturers protocol (GE Healthcare). Specificity of the cloned mAb was determined by ELISA.
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bioRxiv - Microbiology 2022Quote: ... The complex was then purified and excess Fab92 removed by SEC using a Superose 6 Increase 10/300 column (GE Healthcare), equilibrated with 10 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were concentrated using centrifugal concentrators (Amicon, 100 kDa) and polished by size exclusion chromatography (SEC, Superose 6 Increase, GE Healthcare) in buffer containing 25 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... Aggregates were removed by centrifugation (20 min, 48000xg, 4° C) and the protein solution loaded onto a 6 ml Resource S column (GE Healthcare) equilibrated in 20 mM MOPS-NaOH pH 7.0 ...
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bioRxiv - Biochemistry 2022Quote: ... TEV proteases were then added to the eluted proteins to remove the GFP and strep tag before the elution was concentrated by a 50-kDa MWCO spin concentrator and injected onto a Superose 6 column (GE Healthcare) equilibrated in 100 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... The protein was further concentrated by a 100-kDa cutoff concentrator (Milipore) and loaded onto a Superose 6 increase 10/300 column (GE Healthcare) running in lysis buffer plus 0.03% digitonin ...
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bioRxiv - Biochemistry 2022Quote: ... EGTA-eluates were concentrated and further purified by size exclusion chromatography (SEC) on a Superose 6 increase 10/300 GL column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was cleared by consecutive centrifugation at 5,000 and 18,000 rpm and incubated with IgG Sepharose 6 Fast Flow beads (GE Healthcare; Cat# 17096902) on a rotating wheel for 120 min at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Fractions containing the proteins were pooled and further purified by size exclusion chromatography using a Superose 6 10/300 column (GE Healthcare) equilibrated with storage buffer ...
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bioRxiv - Cell Biology 2022Quote: ... sjRop1 LPPs and the purified REEP1 proteins from the membrane fractions were further purified by size exclusion chromatography (SEC) on Superose 6 or Superdex 200 (GE Healthcare) columns ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 4 °C) and the supernatant was incubated with 75 μl of IgG Sepharose 6 Fastbeads (GE Healthcare, 52-2083-00 AH) by rotating at 4 °C for 2 to 3 hours to enable binding of the tagged protein ...
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bioRxiv - Microbiology 2022Quote: ... the supernatants were harvested and filtered for further purification by Histrap HP (GE) and Superose 6 increase 10/300 column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... All FLAG-tagged variants were concentrated using centrifugal concentrators (Amicon, 30 kDa molecular weight cut-off (MWCO)) and polished by size exclusion chromatography (SEC, Superose 6 Increase, GE healthcare) in SEC1 buffer (30 mM HEPES/KOH ...
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bioRxiv - Molecular Biology 2022Quote: ... the pre-cleaning step was omitted and the ChIP extract directly incubated o/n on a rotor at 4°C with 50 μl IgG Sepharose 6 Fast Flow beads (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... 3 mg of proteins was used for immunoprecipitation with 50 ml of immunoglobulin G-Sepharose resin (IgG Sepharose 6 Fast Flow, GE Healthcare) or Anti-HA affinity matrix beads (Roche ...
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bioRxiv - Immunology 2022Quote: ... Conjugated S-mi3 nanoparticles were separated from uncoupled S-6P trimers by SEC using a Superose 6 10/300 column (GE Healthcare) equilibrated with PBS ...
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bioRxiv - Biochemistry 2022Quote: ... were solubilised in TK buffer supplemented with 6 M urea before loading onto a 5 ml HisTrap HP column (GE Healthcare). The column was first washed with TK + 6 M urea followed by TK + 6 M Urea + 50 mM imidazole ...
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bioRxiv - Cell Biology 2023Quote: ... Analytical size-exclusion chromatography was carried out under isocratic conditions on a Superose 6 5/150 or Superdex 200 5/150 (GE Healthcare) equilibrated with SEC buffer (50 mM HEPES pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... SDS-gels were stained by “Blue-Silver” Coomassie Staining (Candiano et al., 2004) and washed with water for 6 hours or overnight before imaging with LAS4000 (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by size exclusion chromatography (SEC) using a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated with Buffer A ...
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bioRxiv - Biochemistry 2022Quote: ... The fractions that contained the protein were concentrated and further purified at 4 °C on a preparative Superose 6 XK 16/70 size exclusion column (GE Healthcare) equilibrated with 25 mM HEPES.Na pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... 500 µg clarified supernatant from P2 fractions was further fractionated by size-exclusion chromatography (SEC) on a Superose 6 increase column (GE Healthcare) equilibrated with LB buffer (25 mM HEPES pH 7.4 ...
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bioRxiv - Biophysics 2023Quote: ... Samples were injected on the Äkta prime plus chromatography system (Cytivia) and separated on a Superose 6 10/300 column (GE Healthcare) in SEC buffer (10 mM HEPES ...
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bioRxiv - Neuroscience 2023Quote: ... and pellets were resuspended in 6 mL 25 % Standard Isotonic Percoll (Percoll: GE Healthcare, 17-5445-02 with 10% 10x PBS) in Medium A ...
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bioRxiv - Cell Biology 2023Quote: ... The GST-tagged Bridge domain was purified by affinity chromatography with glutathione-SepharoseTM 4B resins (Cytiva) followed by size exclusion chromatography with Superose 6 10/300 (GE Healthcare) in the running buffer containing 150 mM NaCl and 20 mM HEPES (pH 7.5).
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bioRxiv - Biophysics 2023Quote: ... The His6-SUMO-14-3-3ζ fusion protein was purified by immobilized metal affinity chromatography (IMAC) using Ni Sepharose 6 Fast Flow resin (5 mL; GE Healthcare) with standard IMAC procedures of sample application ...
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bioRxiv - Biophysics 2023Quote: ... The mixed complexes were incubated over night at 4 °C and the formed R2SP complex was separated from excess SPAG1/PIH1D2 using a Superose 6 16/60 XK (GE Healthcare), see Supplementary Information for detailed protocol ...
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bioRxiv - Biophysics 2023Quote: ... the protein was diluted 10 times with PBS buffer and immobilized on a Nickel Sepharose 6 Fast Flow resin (GE Healthcare). The resin was washed extensively with milliQ water followed by PBS buffer pH 7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... The concentrated protein sample was centrifuged at 10,000 × g and 4°C for 10 min before the supernatant was loaded onto a lysis buffer equilibrated Superose 6 increase 10/300 GL column (GE Healthcare), connected to a 1260 Bioinert Infinity LC system and equipped with a multi-angle light scattering detector (miniDawn 3141MD3 ...
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bioRxiv - Biophysics 2023Quote: ... The protein was further concentrated by a 100-kDa cutoff concentrator (Milipore) and loaded onto a Superose 6 increase 10/300 column (GE Healthcare) running in lysis buffer with 1 mM MgCl2 and 0.03% digitonin ...
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bioRxiv - Biophysics 2023Quote: ... The protein was further concentrated by a 100-kDa cutoff concentrator (Milipore) and loaded onto a Superose 6 increase 10/300 column (GE Healthcare) running in lysis buffer with 0.03% digitonin ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated with a 100 kDa cutoff concentrator to 500 μL and loaded on a Superose 6 Increase 10/300 column (GE Healthcare) equilibrated with gel filtration buffer (20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... the soluble fraction was passed over the appropriate affinity resin of Strep-Tactin XT Superflow (IBA) or Ni Sepharose 6 Fast Flow affinity resin (GE Healthcare), eluted with wash buffer (50 mM Tris–HCl pH 8.0 ...
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bioRxiv - Biophysics 2023Quote: Size exclusion chromatography (SEC) was performed using ÄKTAprocess 6 mm Std system controlled by the Unicorn 7.0 software (GE Healthcare, Sweden). Sepharose 6 Fast Flow beads (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: Cellular lysates were separated on a Superose 6 HR 10/30 size-exclusion column and an AKTA Purifier protein purification system (GE Healthcare), essentially as described previously(61) ...
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bioRxiv - Biochemistry 2022Quote: ... The ACE2-induced postfusion S-nanodisc sample was purified by gel filtration chromatography on Superose 6 Increase 10/300 column (GE Healthcare) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Biophysics 2023Quote: ... The concentrated protein sample was further purified by size exclusion chromatography (SEC) using a Superose 6 Increase 10/300 GL (GE Healthcare) column pre-equilibrated with the buffer E (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... After His-tag purification protein was subsequently purified by size exclusion chromatography using a Superose 6 Increase 10/300 column (GE Healthcare). The oligomeric state of CsoSCA-MBP was determined from the Superose 6 Increase chromatogram and a calibration standard (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were eluted with HBS containing 200 mM imidazole and further purified using size-exclusion chromatography (Superdex 200 Increase 10/300 or Superose 6 Increase 10/300 columns; GE Healthcare) in 10 mM Tris ...
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bioRxiv - Plant Biology 2023Quote: ... for 2 hours and then extensively washed with lysis buffer and eluted in lysis buffer with 5mM desthiobiotin and further purified by size-exclusion chromatography with Superose 6 increase 10/300 GL-column (GE Healthcare).
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bioRxiv - Immunology 2023Quote: ... Plasma lipoproteins were separated using fast-performance liquid chromatography with two Superose 6 10/300 GL columns (GE Healthcare; Boston, MA) on a Shimadzu HPLC system (Columbia ...
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bioRxiv - Immunology 2023Quote: ... Affinity-purified proteins were incubated with HRV-3C protease to remove purification tags and subsequently purified by size-exclusion-chromatography on a Superose 6 Increase column (GE Healthcare) in TBS
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bioRxiv - Immunology 2023Quote: ... protein was purified using cell-free culture supernatant by StrepTactin resin (IBA) and by size-exclusion chromatography using Superdex 200 (RBD and NTD) or Superose 6 (S-2P) column (GE Healthcare) in 10 mM Tris pH8 ...
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bioRxiv - Cell Biology 2023Quote: ... Elution was performed with imidazole and the second step of purification was performed using Superose 6 Increase 10/300 GL gel filtration chromatography (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... The harvested cells were lysed by pulsed sonication and the soluble fraction was affinity purified using Ni2+-Sepharose (Ni Sepharose 6 Fast Flow, GE Healthcare). The resulting MsLadC variants ...
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bioRxiv - Biophysics 2023Quote: ... The 6×His-tagged SUMO fusion proteins were purified using a HisTrap™ HP Ni2+ affinity column (GE Healthcare Life Sciences). The fractions containing fusion protein were pooled and exchanged into a SUMO protease cleavage buffer (20 mM Tris-HCl at pH 8.0 ...
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bioRxiv - Biophysics 2023Quote: ... The eluted sample was concentrated using an Amicon Ultra Centrifugal Filter (MWCO 100 kDa) and purified by Superose 6 Increase 10/300 column (GE Healthcare) pre-equilibrated with buffer containing 20 mM HEPES pH 7.5 ...