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Citations for GE Life Sciences :
1501 - 1550 of 2256 citations for 5 2 3 Difluorophenyl 5 oxovaleric acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... cleared lysates from ARL15A86L expressing Flp-In™ T-REx™ HeLa cells were incubated with streptavidin beads (17-5113-01, 5 ml, GE Healthcare). Trypsin digestion on beads was performed and peptides were collected in water ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was injected into a Ni2+-NTA agarose affinity column (containing 5 mL Ni2+-Sepharose 6 FF beads, GE Healthcare, Cat. 17531803). The column was incubated for 15 minutes and washed twice with 30 mL washing buffer (50 mM Tris ...
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bioRxiv - Cell Biology 2021Quote: ... The samples loaded on SDS-PAGE and γ-32P phosphorylation signals were detected using Amersham Typhoon 5 Biomolecular Imager (GE Healthcare Life Sciences).
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bioRxiv - Biophysics 2022Quote: ... Purification of resulting protein was performed by immobilized metal affinity chromatography using a 5 mL HisTrap HP column (GE Healthcare, Frei-burg, Germany) containing HisTrap chelating stationary phase (Amersham Biosciences ...
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bioRxiv - Biochemistry 2022Quote: ... The crude extract was applied to an immobilized metal ion affinity chromatography column (HisTrap FF, 5 mL resin; GE Healthcare, Little Chalfont, UK). After washing with 25 mL of the 1 x T-buffer containing 25 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... The precipitated pellet was resuspended in Buffer A and the solution subjected to Ni(II) immobilized affinity chromatography using a 5 mL HisTrap FF column (GE Healthcare Life Sciences) with a gradient from 100% buffer A to 100% buffer B (25 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... The gel was exposed with phosphor imaging screens for 5 minutes before being scanned with a Typhoon™ FLA9500 (GE Healthcare Life Sciences). The bands corresponding to the probes were cut out of the gel and conserved at -20°C for future work ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatants were applied to two connected Strep-Trap HP 5 ml columns packed with Strep-Tactin ligand immobilized in an agarose matrix (GE Healthcare Life Sciences) previously equilibrated with equilibration buffer (50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Biophysics 2021Quote: ... The supernatant was then vacuum filtered and loaded at 1 mL/min onto a HiTrap Q HP column (5 mL, GE Healthcare Life Sciences) pre-equilibrated with buffer D [10 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... proteins were transferred to nitrocellulose membranes and incubated with streptavidin-AlexaFluor-647 followed by fluorescence detection with a Cy5 filter (excitation/emission: 649/670 nm) with an Amersham Typhoon 5 Biomolecular Imager (GE Healthcare Life Sciences). FY01 contains a Cy5 fluorophore ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was filtered (0.45 μm) and the target protein purified via immobilized metalion affinity chromatography (IMAC) using a 5 mL HisTrap™ HP column (GE Healthcare, UK). The lysis buffer (50 mM Tris/HCl ...
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bioRxiv - Synthetic Biology 2021Quote: ... The supernatant was then collected for purification using a 5 mL His Trap™ FF Crude Column containing Ni-NTA (GE Healthcare, USA). The column was first equilibrated using five volumes of His-tag Buffer + 10 mM imidazole and the supernatant was loaded into de column ...
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bioRxiv - Immunology 2021Quote: ... containing 5% non-fat milk (5% milk TBS-N) for 1 hour and probed with primary and HRP-conjugated secondary antibodies (GE Healthcare, 1:3000) diluted in 5% milk TBS-N for 1 hour at room temperature or overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were washed three times for 5 min in 0.05% PBST at RT and protein bands were subsequently visualized by ImageQuant™ LAS 4000 (GE Healthcare Life Sciences) using EZ-ECL Chemiluminescence detection kit for HRP (Biological Industries ...
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bioRxiv - Immunology 2020Quote: ... Fractions that yielded proliferation of TN T cells were pooled and subjected to cation exchange chromatography on a Mono S 5/200 GL column (GE Healthcare, Piscataway NJ). The active fractions were again pooled and subjected to buffer exchange (Amicon ultra 3k cutoff ...
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bioRxiv - Biophysics 2020Quote: ... DCS experiments with working buffer solutions containing 4 μM of an SBD-mutant and 5 mM substrate were conducted on a VP-DSC Calorimeter (Micro Cal™, GE Healthcare, Buckinghamshire). The melting temperature Tm was determined by ORIGIN 8 (Origin Lab Corp ...
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bioRxiv - Molecular Biology 2023Quote: ... the array was washed 3 times for 5 minutes with 1X TBS-T solution incubated with the secondary antibody solution anti-rabbit HRP (Na934v, GE Healthcare; 1:5000) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2023Quote: Analytical size exclusion HPLC of purified complexes of Af1:7B8 and Af2:7B8 were carried out using a Superdex 200 5/150 GL column (GE Healthcare, Freiburg, Germany) equilibrated in PBS ...
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bioRxiv - Bioengineering 2023Quote: ... samples were washed trice with 1× DPBS for 5 min each and then washed thrice for 20 min using HyPureTM molecular biology grade deionized distilled (DDI) water (GE Healthcare Life Sciences). Next ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing the tagged domain were pooled and exchanged to the initial buffer containing 5 mM imidazole by using a PD10 desalting column (GE Healthcare Life Sciences). His-tagged TEV protease (0.1 mg/ml final concentration ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: we incubated samples for 10 min with Buffer A (1 M GA, 80% ACN, 5% TFA) followed by incubation with the TiO2 Mag Sepharose (GE Healthcare Life Sciences) for 30 min in an Eppendorf Thermomixer at 800 rpm ...
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bioRxiv - Molecular Biology 2023Quote: ... WT and C111S constructs were extracted from harvested cells by homogenization and soluble fractions loaded on a 5 ml HisTrap FF Crude column (GE Healthcare Life Sciences) equilibrated in binding buffer [20 mM Tris pH8.0 ...
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bioRxiv - Microbiology 2023Quote: ... Unencapsulated ANTS and DPX were removed by gel filtration through a 5 mL HiTrap Desalting Sephadex G-25 resin column (GE Healthcare Life Sciences). To ensure the correct size and obtain LUVs ...
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bioRxiv - Biochemistry 2022Quote: ... 45 μl of the protein at 4.43 mg ml−1 (70.4 µM) was injected in SEC column S200i 5/150 (GE Life Sciences, Velizy-Villacoublay, France) with a buffer containing 20mM Hepes pH 7,5 ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was filtered (0.2 µm) and applied onto a Ni-NTA column (HisTrap FF, 5 ml, GE Healthcare Europe GmbH, Freiburg, Germany). Bound protein was washed with 10 column volumes of washing buffer (25 mM TRIS-HCl pH 7.8 ...
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bioRxiv - Microbiology 2022Quote: ... of this fraction was conducted using a 5 mL HiTrap Chelating HP Sepharose column on the ÄKTA FPLC protein purification system (GE Healthcare, Uppsala, Sweden). The proteins were eluted with 10 mL of starting buffer A (native buffer + 10 mM imidazole ...
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bioRxiv - Immunology 2023Quote: ... The products were filtered by 10 kDa-cutoff Amicon Ultra centrifugal filters to remove DNA and proteins and then analyzed by a Mono Q 5/50 GL column (GE Healthcare Life Sciences).
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bioRxiv - Biophysics 2023Quote: ... Cell suspensions were filtered through a 70 µm cell strainer, centrifuged (5 min, 500 g) and pellets were resuspended in DMEM containing 30% stock isotonic Percoll (SIP, GE Healthcare, Diegem, Belgium). Hereafter ...
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bioRxiv - Biochemistry 2024Quote: ... The filtered soluble cell solution was passed through an equilibrated Nickel-charged IMAC using a 5 mL HisTrap HP (GE Healthcare Life Sciences) in Lysis Buffer ...
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bioRxiv - Synthetic Biology 2024Quote: ... Clarified supernatant containing TetR was then purified using His-tag affinity chromatography with a Ni-NTA column (HisTrap FF 5 ml column, GE Healthcare Life Sciences) followed by size-exclusion chromatography (Superdex HiLoad 26/600 200 pg column ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was subjected to TEV cleavage to remove the GST tag and then further purified using a Mono Q 5/50 GL anion exchange column (GE Healthcare Life Sciences). Finally ...
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bioRxiv - Physiology 2024Quote: ... and a multi-frequency linear-array probe (12 L-RS, 5 – 13 MHz, 38.4-mm field of view, GE Healthcare, Milwaukee, WI, USA) were used to obtain ultrasonography images of the dominant VL and RF (based on kicking preference ...
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bioRxiv - Biochemistry 2024Quote: ... The diluted soluble fraction was then loaded at 1 mL/min in Buffer A onto a 5 mL HiTrap SPFF column (GE Healthcare Life Sciences) and eluted at 2 mL/min with a 70 mL gradient to 100% Buffer B ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was filtered (0.45 μm filter) and loaded on a 5 mL HisTrap Fast Flow column (GE Healthcare, 17-5255-01). The purification was conducted on the bench using a peristaltic pump ...
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bioRxiv - Cancer Biology 2021Quote: ... pH 3-10 (GE Healthcare) and peptides focused for 20 kVh ...
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bioRxiv - Cancer Biology 2021Quote: ... pH 3-10 (GE Healthcare) diluted 1:50 in 5% glycerol ...
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bioRxiv - Neuroscience 2021Quote: ... The slides were dried for 2-3 hours at 30°C and exposed to a Tritium Phosphor Screen (GE Healthcare, Fuji BAS-TR 2025 E) together with calibrated tritium standards (American Radiolabeled Chemicals ...
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bioRxiv - Immunology 2020Quote: HEK293T cells were grown at 37°C to approximately 80% confluence in 10 cm tissue culture dishes using DMEM with 5% Hyclone Fetal Clone II (GE Healthcare Lifesciences, Logan, UT) and 1X antibiotic and antimycotic (Gibco ...
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bioRxiv - Biochemistry 2021Quote: Nickel Immobilised Metal Ion Affinity chromatography (Ni2+-IMAC) for protein purification was conducted using a HisTrap® HP 5 mL column (GE Healthcare, Munich, Germany) coupled to a BioLogic DuoFlow™ Chromatography System (Bio-Rad ...
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bioRxiv - Biophysics 2021Quote: ... The culture medium containing the secreted ACE2 was subsequently loaded onto an ion-exchange column (HiTrap 5 ml Q FF anion exchange chromatography column; GE Healthcare, U. S. A.), and subsequently eluted by a linear salt gradient ranging between 10 and 1000 mM NaCl ...
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bioRxiv - Biophysics 2022Quote: ... were purified by immobilized metal affinity chromatography (IMAC) using a HisTrap HP 5 mL column mounted on an Äkta Purifier System (GE Healthcare, Chicago, IL, USA). Fractions containing the His6-Sumo-peptide fusion in elution buffer (20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed three times with PBST for 5 min and incubated for 1 h at room temperature with HRP-conjugated secondary anti-rabbit IgG (GE Healthcare, NA934, 1/2000) or anti-mouse IgG (Amersham ...
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bioRxiv - Genomics 2020Quote: ... The mixture was left to react for 30 minutes protected from ambient light and the crude reaction mixture was passed through a NAP-5 column (GE Healthcare Life Sciences, 17085301) for collection and purification of the fluorophore-conjugated secondary antibody ...
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bioRxiv - Microbiology 2020Quote: ... Filtrates were then filtered gently by vacuum (<400 mbar under-pressure) over pre-combusted GF/A filters (> 5 h at 460°C; 47 mm, GE Healthcare Whatman, Buckinghamshire, UK), and finally over 0.22 µm pre-cleaned44 hydrophilic PVDF filters (<600 mbar under-pressure ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were purified in two steps by immobilized metal chelate affinity chromatography (IMAC) using 5 mL HisTrap FF columns (GE Healthcare, Chicago, Illinois, USA) as previously described ...
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bioRxiv - Biophysics 2020Quote: ... SEC was conducted at 4°C using a Superdex 200 5/150 column on an AKTA Pure System (GE Healthcare Life Sciences, Marlborough, MA) with a flow rate of 0.15 mL/min and 0.5 s exposures ...
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bioRxiv - Cell Biology 2023Quote: ... recombinant desmin was purified under denaturing conditions (8 M urea) by ion exchange chromatography (IEC) using 5 mL HiTrap DEAE-FF columns (GE Healthcare, Chicago, IL, USA) and in a second step by immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Neuroscience 2024Quote: ... We separated equal amounts of protein on 5-20% SDS‒PAGE gels (Wako, Osaka, Japan) and transferred them to Hybond-P membranes (GE Healthcare, Piscataway, NJ, USA). The following primary antibodies and dilutions were used ...
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bioRxiv - Immunology 2024Quote: ... The wells of the plates were then washed 5 times with PBS-T and incubated with a 1:5000 dilution of anti-mouse IgG-HRP (GE healthcare cat. # NA931-1ml), or with a 1:100000 dilution of anti-ferret IgG-HRP (Abcam cat ...
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bioRxiv - Molecular Biology 2023Quote: ... The Fc portion and uncut IgG was removed by flowing through a 1 mL or a 5 mL MabSelect SuRe column (GE Healthcare #11-0034-95) in PBS (Fab flows through the column) ...