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Citations for GE Life Sciences :
1401 - 1450 of 2256 citations for 5 2 3 Difluorophenyl 5 oxovaleric acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The lysate was cleared by centrifugation and loaded onto two serially connected HisTrap 5 ml columns (Cytiva) which were connected to a ÄKTA FPLC system (GE Healthcare). Columns were washed with lysis buffer and bound proteins were eluted with a linear imidazole gradient (20 – 500 mM) ...
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bioRxiv - Microbiology 2024Quote: ... Human monocyte cell line U937 was maintained at 37°C in 5% CO2 and RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Hyclone, GE Healthcare). Differentiation of U937 monocytes in macrophages was induced with 100 ng/ml phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Immunology 2024Quote: ... supernatants underwent protein affinity purification using a high-performance 5 mL HiTrap MabSelect SuRe column (Cytiva, catalog no. 11003494) from GE Healthcare Life Sciences for large-scale production ...
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bioRxiv - Biochemistry 2024Quote: ... at 8000 × g then subjected to two rounds of buffer exchange using equimolar Tris-KCl buffer and a 5-mL desalting column (HiTrap Desalting; GE Healthcare) pre-equilibrated with four column volumes of Tris-KCl ...
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bioRxiv - Biochemistry 2024Quote: ... Here 70 µL of purified AaSiaP protein at 10 mg mL−1 was injected onto an inline Superdex S200 Increase 5/150 GL (GE Healthcare) SEC column ...
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bioRxiv - Biochemistry 2024Quote: ... The cleaved sample was passed through an equilibrated Nickel-charged IMAC using a 5 mL HisTrap HP (GE Healthcare Life Sciences), and the flowthrough was collected ...
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bioRxiv - Molecular Biology 2024Quote: ... Eluent was combined with an additional 2x 20 mL of wash buffer and subsequently applied to a 5 mL HiTrap Q (GE Healthcare) ion-exchange column equilibrated in a buffer containing 25 mM Tris•Cl ...
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bioRxiv - Biochemistry 2024Quote: ... DsbA-RbmB was purified from the cleared lysate by loading the supernatant onto a cobalt-charged 5 mL His-trap HF (GE Healthcare) equilibrated with high-salt TBS buffer (20 mM Tris pH 7.6 ...
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bioRxiv - Biochemistry 2024Quote: ... Dried gels were then exposed to a storage phosphor screen for six days before acquiring radiograms using a Typhoon 5 Imager (GE Healthcare). All experiments were performed in three independent replicates that were carried out on three separate days ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was filtered through a 0.45 µm membrane and loaded onto a nickel affinity column (5 mL GE Healthcare HisTrap). The protein was eluted using a gradient from 20 to 500 mM imidazole (solution B ...
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bioRxiv - Biophysics 2024Quote: ... The eluate was concentrated with 5 kDa spin concentrator and loaded on a Superdex 200 Increase 10/300 column (GE Healthcare) using 20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... Refolded and soluble proteins were concentrated using Vivaspin 20 centrifugal concentrators (10 kDa and 5 kDa molecular weight cutoff) and further purified on Superdex S75 columns (GE Healthcare) in SEC buffer (50 mM HEPES ...
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bioRxiv - Cell Biology 2024Quote: ... Clarified lysates were loaded using a sample pump or super-loop (Cytiva) on a 5 mL Strep-Trap HP column (GE Healthcare) which was pre-equilibrated with 5 column volumes (CV ...
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bioRxiv - Cell Biology 2024Quote: ... the protein was separated from MBP using cation-exchange chromatography with a 5 ml SP Sepharose HiTrap column (GE Life Sciences). Fractions containing dCas9 protein were pooled and the protein was concentrated with spin concentrators (Amicon Ultra 15 ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated to 2-3 mL for separation on exclusion size chromatography (HiLoad 16/600 SuperdexTM 75pg, GE Healthcare; ÄKTA Avant). Finally ...
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bioRxiv - Cancer Biology 2021Quote: ... a cell suspension containing 20,000 cells per 50uL was prepared in Biolog IF-M1 containing 0.3mM glutamine and 5% dialyzed FBS (dFBS) (Hyclone GE Life Sciences, SH30079.01) and plated into PM-M1 and PM-M2 96-well plates at 50 μL and 20,000 cells per well ...
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bioRxiv - Cell Biology 2020Quote: ... Blots were rinsed twice and washed 4 x 5 minutes in 1X TBST and developed using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare RPN2232), according to the manufacturer’s suggestions ...
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bioRxiv - Neuroscience 2021Quote: ... Lipofectamine 2000 and plasmidic DNA were left to complex for 20 minutes at room temperature and added to the plated BV2 cells in low glucose DMEM supplemented with 5% fetal bovine serum (FBS, GE Healthcare Hyclone) and no antibiotics to ensure full transfection efficiency ...
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bioRxiv - Neuroscience 2020Quote: ... Lipofectamine 2000 and siRNAs were left to complex for 20 minutes at room temperature and added to the plated BV2 cells in low glucose DMEM supplemented with 5% fetal bovine serum (FBS, GE Healthcare Hyclone) and no antibiotics to ensure full transfection efficiency ...
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bioRxiv - Cell Biology 2021Quote: ... Figure 5F and Figure 5-figure supplement 1D were acquired and processed by iterative deconvolution using Soft-WoRx software (Applied Precision Ltd.). For Figure 1E ...
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bioRxiv - Bioengineering 2021Quote: ... The dialyzed eluate was further passed through an equilibrated 5 mL heparin column (HiTrap™ Heparin HP) on a FPLC (AKTA pure, GE healthcare) and eluted using a linear NaCl gradient generated from heparin buffers A and B (40 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... that had been equilibrated in NiNTA A buffer followed by buffer exchange into SEC buffer using a 5 mL HiTrap® Desalting Column (GE Healthcare). Fractions containing protein of interest were pooled ...
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A sound strategy for homology modeling-based affinity maturation of a HIF-1α single-domain intrabodybioRxiv - Biochemistry 2020Quote: ... Purification was performed by combing a HisTrapFF column and analytical size-exclusion chromatographic Superdex™ 75 5/150 with an ÄKTAexplorer 100 (GE Healthcare). The human cDNA sequence for the HIF-1α-PAS-B domain was synthesized and subcloned into the prokaryotic expression vector pGEX-4T-1 (Amersham) ...
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bioRxiv - Biochemistry 2020Quote: ... Both the LaGH13_20 and LaGH13_31B enzymes were purified by immobilized metal ion affinity chromatography on 5 ml HisTrap HP columns (GE Healthcare, Uppsala, Sweden) equilibrated with binding buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... diluted two-fold against fresh buffer B and then manually applied at room temperature to a 5 mL HiTrap Heparin cation exchange column (GE Healthcare # 17040701) that had been pre-equilibrated in buffer B ...
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bioRxiv - Biochemistry 2020Quote: ... The protein purification from precipitated cell-culture media was done on HisTrap HP 5 ml and HiLoad 16/600 Superdex 75 (GE Healthcare, USA) columns by the method described previously (Zahradník et al. ...
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bioRxiv - Biophysics 2021Quote: ... Bacterial cells were disrupted by sonication and the soluble fraction was loaded on a 5 mL HiTrap Chelating HP column (GE Healthcare - USA) previously equilibrated with same buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Protein samples at concentrations >5 mg/ml were loaded onto a Superdex™ 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... The modified Cy3 labeled fragment was generated by labeling an amine-modified RNA containing a 5′ monophosphate ordered from Horizon Discovery with monoreactive Cy3 (GE Life Sciences) and the labeled RNA was purified by reverse-phase HPLC ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5 % (v/v) glycerol by FPLC (ÄKTA explorer, Amersham Pharmacia Biotech) using a Superdex™ 200pg 16/60 FPLC column (GE Healthcare). Final protein fractions were analyzed by SDS-PAGE electrophoresis.
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bioRxiv - Cell Biology 2021Quote: ... 10% (v/v) glycerol and 1 mM DTT) and injected onto a MonoQ 5/50 GL column (GE Healthcare and Life Sciences) at 1 mL/min ...
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bioRxiv - Biophysics 2022Quote: ... coli upec-117 CapW at 5 mg/mL was injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in buffer GF ...
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bioRxiv - Biochemistry 2022Quote: ... Protein samples at concentrations >5 mg/ml were loaded onto a Superdex™ 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The combined eluants were incubated overnight at 4 °C with 5 mL of Glutathione-Sepharose 4B resin (GE Healthcare Bio-sciences AB). The beads were placed in a column and washed 3 times with 50 mL of Buffer B (480 mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... filtered through a 0.22 μm membrane filter and loaded onto a 5 ml HiTrap Heparin HP column (GE Healthcare, Chicago, Illinois, USA). The column was washed with 10 volumes of Buffer H (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... 10% glycerol at pH 8.2) and loaded onto a 5 mL prepacked Q-sepharose ion exchange column (GE Healthcare Life Sciences, MA) at 1 mL/min ...
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bioRxiv - Immunology 2020Quote: ... The supernatant was harvested 5 days post transfection and IgG was affinity purified using Protein A Sepharose (GE Healthcare, #17-5280-02). SDS-PAGE was used to confirm the purity of IgG and adequate cleavage of the P2A peptide ...
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bioRxiv - Bioengineering 2021Quote: Several DSAs were performed to guide the intervention by injecting approximately 5-10 ml of a half-strength contrast agent (CA) (50% of Omnipaque 240 (GE Healthcare, USA) and 50% of a saline solution ...
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bioRxiv - Microbiology 2021Quote: ... Fusion events were quantified by detecting GFP+ pixels after 18 hours incubation at 37°C 5% CO2 using Amersham™ Typhoon™ Biomolecular Imager (channel Cy2; resolution 10μm; GE Healthcare). Data was analyzed using the ImageQuant TL 8.2 image analysis software (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then collected by vacuum filtration onto glass microfiber filters (grade GF/C, 125 mm in diameter, pre-combusted at 460°C for > 5 h, GE Healthcare Whatman). The filters were transferred to 50 mL centrifuge tubes (Sarstedt ...
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bioRxiv - Genomics 2020Quote: ... mRNA was extracted from approximately 5 μg of the total RNA samples using Sera-Mag oligo-dT beads (GE Healthcare Life Sciences). The mRNA was fragmented and used to prepare sequencing libraries according to the Illumina TruSeq Stranded mRNA sample preparation kit ...
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bioRxiv - Cancer Biology 2020Quote: ... + RNA was first denatured by heating at 65°C for 5 minutes and then transferred to A cellulose nitrate membrane (Amersham, GE Healthcare, US) using A Bio-dot device (US Bio-RAD).The membranes were then uV-crosslinked ...
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bioRxiv - Biochemistry 2020Quote: ... Nsp12 was then purified by Nickel-affinity chromatography followed by ion-exchange chromatography (using HisTrap FF and Capto HiResQ 5/50 column, respectively. GE Healthcare, USA). Nsp12 eluates within conductivity of 19-23 mS/cm were combined and injected onto a Superdex 200 increase 10/300 GL column (GE Healthcare ...
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bioRxiv - Systems Biology 2021Quote: ... GTP-loaded protein was buffer exchanged into either NMR buffer or the GTPase assay buffer using NAP-5 Sephadex G-25 DNA Grade columns (GE Healthcare # 17085301). We were unable to obtain sufficient material for some mutants (H141E/I ...
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bioRxiv - Microbiology 2020Quote: ... Fusion events were quantified by detecting GFP+ pixels after 18 hours incubation at 37°C 5% CO2 using Amersham™ Typhoon™ Biomolecular Imager (channel Cy2; resolution 10μm; GE Healthcare). Data was analyzed using the ImageQuant TL 8.2 image analysis software (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... Rabbit sera were shipped to our laboratory where purification of IgG was performed by affinity chromatography using a 5-ml HiTrap protein A HP column following manufacturer’s instructions (GE Healthcare, Piscataway, NJ). Purified IgG protein concentration was determined by Nanodrop ND-1000 spectrophotometer ...
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bioRxiv - Microbiology 2020Quote: ... The protein (5-6 ml) was further purified by FPLC over a 26/60 Superdex 200 pg size exclusion column (GE Life Sciences) equilibrated with 20 mM Tris (pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: The supernatant fraction containing the rhGFAT2 protein (with or without tag) was subjected to purification using a Ni+2NTA affinity column (HisTrap HP 5 mL, GE Healthcare, USA). The column was equilibrated with 10 column volumes (CV ...
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bioRxiv - Biochemistry 2020Quote: ... The dialyzed protein was diluted in buffer C to reach 50 mM NaCl immediately before loading to a Q-sepharose HP column (5 mL, GE Healthcare, USA), previously equilibrated with 10 CV of Buffer C ...
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bioRxiv - Biochemistry 2021Quote: ... filtered (0.22 mm) and then applied to 5 mL HiTrap Chelating HP columns (GE Healthcare, protein from 6 L cells per column) charged with Ni2+ and equilibrated with 4 column volumes of buffer C (20 mM Tris-HCl ...