Labshake search
Citations for GE Life Sciences :
1501 - 1550 of 2341 citations for 3 Chloro 5 fluoro 4' morpholinomethyl benzophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2022Quote: ... 50 μl of each sample (at a concentration of 3 mg·ml−1) were loaded onto either a Superdex 75 or 200 HR 10/300 GL columns (GE Healthcare, Madison, WI, USA), equilibrated in 25 mM Hepes (pH 7.8) ...
-
bioRxiv - Biochemistry 2022Quote: ... lysates in PBS with protease and phosphatase inhibitor cocktails were injected (500 µL per injection, corresponding to 1–3 mg total protein) onto a Superose® 6 10/300GL column (GE Life Sciences) equilibriated with PBS (pH 7.2 ...
-
bioRxiv - Molecular Biology 2022Quote: ... the supernatant was transferred to a new tube and mixed with 20 μl of home-made SPRI beads (equivalent of 1 ml of Sera-Mag Magnetic SpeedBeads, carboxylated, 1 μm, 3 EDAC/PA5 (GE Healthcare Life Sciences) in 50 ml of binding buffer containing 10 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Immunology 2022Quote: ... Aliquots of the purified A48 or D48 PTX3 proteins were run under denaturing conditions on Tris-Acetate 3–8% (w/v) and Bis-Tris 10% (w/v) protein gels (GE Healthcare Life Sciences), in the absence and presence ...
-
bioRxiv - Plant Biology 2020Quote: ... Proteins were isolated and used for the separation in the first dimension with Immobiline Dry Stripes (pH range 3-10 NL, 18 cm, GE Healthcare, Uppsala, Sweden) [20] ...
-
bioRxiv - Biochemistry 2020Quote: ... The fractions were collected and concentrated to a final concentration of 700 µM using Vivaspin sample concentrators (GE Healthcare; cut-off: 3 kDa). The protein purity was analysed by SDS-PAGE using 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad).
-
bioRxiv - Bioengineering 2023Quote: All experiments were performed on a 3 Tesla whole-body GE Premier system equipped with a 21-channel receive head coil (GE Healthcare, Milwaukee, WI). We used fluoroptic probes for the gold standard temperature measurement with a precision of 0.1 °C and a temporal resolution of 1 second (STB Medical ...
-
bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated to 2-3 mL for separation on exclusion size chromatography (HiLoad 16/600 SuperdexTM 75pg, GE Healthcare; ÄKTA Avant). Finally ...
-
bioRxiv - Molecular Biology 2023Quote: ... nitrocellulose membranes were blocked in 3 % BSA in TBST and stained with HRP-conjugated streptavidin (GE Healthcare Life Science, Cat# RPN1231vs,1:2500) in blocking solution ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Positive RDT samples were then used to create dried blood spots (DBS) on 3 mm thick Whatman® filter paper (Whatman, GE Healthcare, UK). The DBS was dried at room temperature ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1 μg of purified DNA was labelled for 3 h at 15°C with fluorescent dUTPs (SpectrumOrange and SpectrumGreen from Abott Molecular and Cy5-UTPs from GE HealthCare Life Science).The templates used in this study are ...
-
bioRxiv - Neuroscience 2024Quote: ... participants were scanned for 45 minutes on a GE SIGNA Premier 3 T high-field scanner with a 48-channel head coil (GE Healthcare, Waukesha, WI), followed immediately by a 1-hour protocol on a Hyperfine Swoop 64 mT ultra-low-field scanner (Hyperfine Inc. ...
-
bioRxiv - Immunology 2024Quote: ... The blots were washed for 3 times in TBST for 10 minutes each and developed using an ECL Plus kit (GE Healthcare, Freiburg, Germany). For quantification of protein intensities by densitometry ...
-
bioRxiv - Cell Biology 2024Quote: ... Membranes were washed 3 times again and the chemiluminescence signal was captured by using ECL reagents (RPN2232, GE Healthcare; 34095, Thermo Fisher Scientific). The following primary antibodies were used ...
-
bioRxiv - Biophysics 2021Quote: ... The cell lysates were centrifuged at 34,000 × g for 20 min at 4°C and the supernatant containing the His-tagged fusion protein was loaded onto a HisTrap column (AKTA-prime, GE Healthcare). The protein was eluted with a buffer containing 20–500 mM linear imidazole gradient ...
-
bioRxiv - Biochemistry 2020Quote: ... The lysate was clarified by centrifugation (15000 rpm for 1 h at 4 °C) and incubated with glutathione Sepharose 4B (GE Healthcare) for 4 h at 4 °C ...
-
bioRxiv - Microbiology 2020Quote: ... The clarified cell lysates were incubated for 2 h on a spinning wheel at 4°C with 100µl of StrepTactin Sepharose High Performance beads (#28935599, GE Healthcare, USA). Beads were collected by centrifugation (1600 g for 5 min at 4°C ...
-
bioRxiv - Cell Biology 2020Quote: ... Antibody-antigen complexes were recovered by incubating for 1 h at 4°C with Protein A-Sepharose CL-4B (GE Healthcare) and eluted with Laemmli sample buffer ...
-
bioRxiv - Cell Biology 2020Quote: ... The cell lysate made by sonication was centrifuged at 11,000 rpm for 30 min at 4◦C and the clear supernatant was purified through Ni-NTA column chromatography (GE Healthcare) following kit protocols ...
-
bioRxiv - Genetics 2021Quote: ... 2D gels were exposed for 4 hours in a phosphor screen cassette and the signal was detected with a Typhoon Trio phosphoimager (GE healthcare).
-
bioRxiv - Molecular Biology 2021Quote: ... and chromatin was released from the resin with 20 µl of ChIP buffer 1 supplemented with 100 µg/ml BSA and 4 µg HRV3C incubated (GE Healthcare) on ice for 60 minutes ...
-
bioRxiv - Cell Biology 2022Quote: ... 150 mM NaCl) and GST-fused proteins were immobilized on Glutathione Sepharose 4 Fast Flow beads (GE Healthcare, #17-5132-01) by incubating in a rotator at 4°C for 1 h ...
-
bioRxiv - Biochemistry 2022Quote: ... The lysate was cleared by centrifugation at 40,000 rpm in a Type 45 Ti rotor for 60 minutes at 4 °C and applied onto a pre-equilibrated HisTrap HP column (GE Healthcare) in CENP-E lysis buffer at 4 °C ...
-
bioRxiv - Biochemistry 2022Quote: ... centrifuged at 13,300 rpm for 15 mins at 4°C and then loaded onto a Superose 6 Increase 10/300 column (GE Healthcare) pre-equilibrated with CENP-E gel filtration buffer (50 mM HEPES pH 7 ...
-
bioRxiv - Molecular Biology 2021Quote: ... The membranes were then incubated with SuperSignal West Dura or ProSignal Pico Spray chemiluminescence substrate for 1-4 minutes at room temperature and imaged using an Amersham Imager 600 (GE Healthcare) or ChemiDoc (Bio-Rad ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1 mM DTT) and addition of 40 µl Protein A Sepharose resin (nProtein A Sepharose 4 Fast Flow, 17-5280, GE Healthcare) that had been blocked for 1 h in ChIP Blocking buffer (ChIP Low salt buffer containing 2 mg/ml BSA and 2% (w/v ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 10-30 μg of protein was electrophoresed on 4-12% (or 12% for OGG1ko blot) Bis-Tris gels (Thermo) before transferring to PDVF membranes (GE Healthcare). Membranes were blocked in 5% milk and blotted with primary and secondary HRP antibodies ...
-
bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by centrifugation at 12000 g at 4 °C for 1 h and loaded onto a HisTrap HP column (GE Healthcare) equilibrated with 98% buffer A/2% buffer B (20 mM Tris ...
-
bioRxiv - Biochemistry 2020Quote: ... FLAG-tagged proteins were purified at 4°C on the anti-FLAG resin at a flow rate of 20 ml/h with a P1 pump (GE Healthcare), and eluted by competition with a FLAG peptide solution at 200 μg/ml in 10 mM Hepes ...
-
bioRxiv - Genetics 2020Quote: ... Whole cell lysates were quantified and 50 μg were resolved in 4-20% SDS-PAGE and transferred onto nitrocellulose membranes (GE healthcare). The membranes were then incubated with the primary antibodies ...
-
bioRxiv - Immunology 2021Quote: ... 1 mM DTT) at 4°C overnight and then human cGAS was further purified on a PD-10 desalting column (GE Healthcare) eluted with storage buffer (20 mM HEPES-KOH pH 7.5 ...
-
bioRxiv - Biophysics 2021Quote: ... Total protein was collected and incubated with anti-p75NTR (Alomone, ANT-007, 1ug) overnight at 4 °C and then incubated with Sepharose protein-G beads (GE Healthcare). Samples were then prepared for immunoblotting as described below.
-
bioRxiv - Biochemistry 2021Quote: ... MBP-PIPI was subjected to HRV-3C cleavage at 4°C overnight and was further purified in HiLoad 16/60 Superdex 200 preparatory grade size exclusion column (GE Healthcare) to separate fractions of polymerase proteins from cleaved MBP and protease ...
-
bioRxiv - Bioengineering 2020Quote: ... Reactions were gently agitated at 4°C overnight prior to removal of unreacted FITC by size exclusion chromatography using PD-10 columns (GE Healthcare). Elution was performed using PBS ...
-
bioRxiv - Neuroscience 2021Quote: ... cortices lysates were immunoprecipitated with anti-Bin1 or mouse IgG 16 h at 4 °C and then with 30 μl of protein G–Sepharose beads (GE Healthcare) for 3 h at 4°C ...
-
bioRxiv - Biophysics 2021Quote: ... Cell debris was removed by centrifugation at 18000*g for 45 min at 4 °C and the supernatant was applied to a Ni2+–Sepharose 6 Fast Flow (GE Healthcare) loaded gravity column ...
-
bioRxiv - Microbiology 2021Quote: ... Precipitation of radiolabeled SARS-CoV-2 Spike glycoproteins from cell lysates or supernatant was performed with CV3-25 in combination with a polyclonal rabbit antiserum raised against SARS-CoV-2 RBD protein for 1 h at 4°C in the presence of 45 μL of 10% protein A-Sepharose beads (GE Healthcare).
-
bioRxiv - Microbiology 2021Quote: ... was incubated with 20-fold excess of CV3-25 Fab overnight at 4°C before purification on a Superose 6 300/10 GL column (GE Healthcare). The complex peak was harvested ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cell pellets were resuspended in 4 ml PBS and subject to density gradient centrifugation with Ficoll-Paque PLUS (17144002, GE Healthcare) according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2020Quote: ... Purification tags were then removed by incubation with 3C protease (10 μg/mg fusion protein) overnight at 4°C followed by passing through tandem Ni2+-NTA and MBP Trap HP columns (GE Healthcare). The flow-through was concentrated as appropriate and loaded on a Superdex 75 26/600 gel filtration column for final purification and buffer exchange into 20 mM HEPES pH 7.5 ...
-
bioRxiv - Plant Biology 2020Quote: ... The membrane was blocked for 1 h at room temperature in TBS containing 4% Amersham ECL Prime Blocking Reagent (GE Healthcare) and 0.1% Tween 20 ...
-
bioRxiv - Systems Biology 2020Quote: ... Lysates were then centrifuged at 20,000 g for 20 min at 4°C and the supernatant loaded to a GSTrap FF 1 mL column (GE Healthcare). Following a washing step with 10 mL GST-Buffer ...
-
bioRxiv - Immunology 2021Quote: ... The affinity-purified protein was TEV-cleaved o/n at 4°C and subsequently subjected to gel filtration on an equilibrated Superdex 75 gel filtration column 16/600 (GE Healthcare). Monomer fractions were pooled and concentrated to 1 mg/ml ...
-
bioRxiv - Cancer Biology 2021Quote: ... was resolved using 4–15% Mini-PROTEAN® TGX™ gels and transferred to Hybond-P 0.45µm PVDF membrane (GE Healthcare). Membranes were blocked in Roche block and incubated with primary antibodies over night or for 3 hours at room temperature ...
-
bioRxiv - Biochemistry 2020Quote: ... The GST tag was cleaved overnight at 4°C by the addition of PreScission Protease (~100 μL at 76 mg/mL) (GE Healthcare) and the eluted ATAD2B bromodomain protein was concentrated to a total volume of approximately 3 mL ...
-
bioRxiv - Microbiology 2021Quote: ... Supernatant was then mixed with GFP resin which was produced by crosslinking nanobody71 to NHS-Activated Sepharose 4 Fast Flow beads (GE Healthcare) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: The purification steps for both POLA1–POLA2–PRIM1–PRIM2 and p50–Ctc1–Stn1–Ten1 were performed at 4°C using an AKTA chromatography system with prepacked columns (GE Healthcare), following the same protocol ...
-
bioRxiv - Biophysics 2020Quote: ... and non-deamidated (pure N373 wildtype) GII.4 P dimer species was achieved by cation exchange chromatography using a 6 ml Resource S column (GE Healthcare) at 6 °C ...
-
bioRxiv - Cell Biology 2021Quote: ... 10 µg of mESC lysate were separated on 4–12% or 10% SDS-PAGE gels (pre-cast NuPage® GE healthcare or Mini-Protean TGX Stain free precast gels ...
-
bioRxiv - Cell Biology 2022Quote: ... incubated in HRP conjugated secondary antibodies detailed in Table 4 for 1 h at room temperature and developed using Pierce SuperSignal Pico Plus (Pierce) or ECL (GE Healthcare) reagent and imaged on ImageQuant.