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Citations for GE Life Sciences :
1451 - 1500 of 4264 citations for Recombinant Human S100A12 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... filtered and purified using protein G affinity chromatography following the manufacturer’s protocol (GE Healthcare).
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bioRxiv - Genetics 2021Quote: ... Protein bands were visualised using Enhanced Chemiluminescence (ECL) Western blotting detection reagents (GE Healthcare) and exposure of the membrane to Kodak BioMax XAR film (Sigma) ...
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bioRxiv - Genetics 2021Quote: ... and proteins were transferred to a nitrocellulose membrane (Amersham Protran 0.45 NC, GE Healthcare).
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bioRxiv - Microbiology 2019Quote: ... Proteins were detected by enhanced chemiluminescence (ECL Prime Western blotting detection reagent, GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... Proteins were separated on a HiLoad Superdex 200 Increase 10/300 column (GE Healthcare) and plotted against BioRad Standards to determine size ...
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bioRxiv - Plant Biology 2021Quote: The purified proteins were loaded onto a Superdex 200 GL 10/300 (GE Healthcare) column and eluted with a buffer containing 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Developmental Biology 2020Quote: ... and proteins were detected using Amersham Western Blotting Detection Reagent (GE Healthcare, Chicago, IL). Western blot membranes were probed with mouse anti-RFP (RF5R Invitrogen ...
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Wnt-dependent activation of ERK mediates repression of chondrocyte fate during calvarial developmentbioRxiv - Developmental Biology 2021Quote: ... Protein was detected using an Amersham ECL Western Blotting Analysis System (GE Healthcare RPN2109), and developed onto film or imaged using a Biorad Chemidoc gel imager ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein purification was performed using Nickel NTA columns (17-5248-01, GE Healthcare, USA) or beads (7066-4 ...
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bioRxiv - Molecular Biology 2021Quote: ... Fc and non-digested IgG were removed via Protein A affinity chromatography (GE Healthcare). 2E10.E9 Fab was subsequently purified via size-exclusion chromatography (Superdex 200 Increase 10/300 GL ...
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bioRxiv - Molecular Biology 2020Quote: ... Then the target proteins were loaded onto a HiTrap Q column (GE Healthcare, USA). An increasing linear gradient of NaCl concentration was applied onto the HiTrap Q column for further purification ...
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bioRxiv - Molecular Biology 2021Quote: ... Total protein levels were quantified with the ImageQuant TL software (GE Healthcare Life Sciences).
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bioRxiv - Molecular Biology 2020Quote: ... protein was immobilized to the CM5 sensor chip (GE Healthcare, Cat#. BR-1005-30) at 6.0 μg/mL concentration using 10mM sodium acetate (pH 5.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... The dialyzed protein was then passed through a Hi-Trap Chelating column (GE Healthcare) and the unbound material was collected ...
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bioRxiv - Molecular Biology 2021Quote: ... cleaved protein was loaded onto a Superdex 75 10/300 GL column (GE Healthcare) in Ni-buffer A ...
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bioRxiv - Bioengineering 2022Quote: ... and IgG was purified with Protein A sepharose beads (GE Healthcare 17-5280-04).
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bioRxiv - Cell Biology 2022Quote: ... 3 mg total protein was loaded on a Superose 6 HR10/30 (GE Healthcare) column equilibrated with PBS ...
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bioRxiv - Genomics 2022Quote: ... Antibody-associated DNA:RNA hybrids were then captured on protein G Sepharose beads (GE Healthcare), washed and purified according to standard ChIP procedures ...
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bioRxiv - Biochemistry 2019Quote: ... the protein was bound to an anion exchange column (MonoQ 16/10, GE Healthcare) and eluted with a linear salt gradient up to 1 M NaCl ...
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bioRxiv - Cancer Biology 2019Quote: ... Cleared lysates were incubated with protein G Mag Sepharose™ Xtra beads (GE Healthcare) and 1µg of antibody anti-ISG15 per sample for 4h ...
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bioRxiv - Cell Biology 2019Quote: GST-tagged mammalian ATG8 fusion proteins were cloned into pGEX-4T-1 (GE Healthcare) and expressed in Escherichia coli BL21 (DE3 ...
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bioRxiv - Synthetic Biology 2019Quote: Western blotting was carried out following protein transfer onto a nitrocellulose membrane (GE Healthcare) on a TE 77 PWR Semi-Dry Transfer Unit (45 mA/gel and 30 min with a NOVA Blot kit ...
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bioRxiv - Biochemistry 2019Quote: ... the protein was loaded onto a pre-equilibrated QHP ion exchange column (GE Healthcare) and eluted with buffer B containing increasing concentrations of NaCl (0.1-1 M) ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were purified by immobilised metal affinity chromatography using nickel-Sepharose resin (GE Healthcare) equilibrated in buffer A ...
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bioRxiv - Plant Biology 2019Quote: ... purified proteins were run down a Superdex™ 75 10/300 column (GE Healthcare) at 0.5 ml/min either alone or mixed to assess complex formation (mixtures were incubated on ice for 2 hours prior to experiment) ...
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bioRxiv - Molecular Biology 2019Quote: ... The immune complexes were then precipitated by Protein G Magnetic Sepharose beads (GE Healthcare) for 4 hours rotating at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... MBP-fusion proteins were purified with amylose resin following the manufacturer’s instructions (GE Healthcare, Pierce ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was passed over a size exclusion column (Superdex 200, GE Life Sciences) in buffer GF (buffer A plus 300 mM NaCl and 1 mM dithiothreitol (DTT)) ...
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bioRxiv - Biochemistry 2020Quote: ... The target protein was further purified using MonoQ and Superdex G75 columns (GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: ... The concentrated proteins were buffer exchanged using a PD-10 desalting column (GE Healthcare) equilibrated with Buffer C (50 mM CAPS ...
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bioRxiv - Plant Biology 2019Quote: ... Proteins were purified using the Sepharose beads affinity method (Glutathione Sepharose 4B; GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: ... Target proteins were further purified on a Superdex-200 16/60 column (GE Healthcare) pre-equilibrated with a running buffer containing 20 mM Tris-HCl (pH 8.0 ...
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bioRxiv - Cell Biology 2019Quote: ... the extracts were incubated with 15 μl of protein A sepharose beads (GE Healthcare) on a rotating wheel at 4°C for 1 h ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were routinely desalted using 5 mL HiTrap™ desalting columns (GE Healthcare, USA) using desalting buffer (50 mM Tris-HCl pH7.4 ...
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bioRxiv - Biochemistry 2019Quote: ... Protein expression was induced by adding (isopropyl β-D-1-thiogalactopyranoside, IPTG; GE Healthcare) to a final concentration of 0.3 mM ...
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bioRxiv - Molecular Biology 2019Quote: ... chromatin lysates were incubated for 4 hours with protein-G sepharose beads (GE Healthcare). Beads were washed 3× with low-salt buffer (150 mM NaCl ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Proteins were concentrated and further purified using gel filtration (S75 16/60, GE Healthcare) and ion-exchange (Hi-Load Q column ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was then passed over a 5 mL HisTrap HP column (GE Healthcare), washed with 10 CV of Nickel Wash Buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was further purified by heparin affinity chromatography (HiTrap Heparin HP, GE Healthcare), eluting with a linear gradient of increasing salt from 150 mM - 1 M NaCl ...
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bioRxiv - Microbiology 2019Quote: ... proteins were transferred onto a nitrocellulose membrane (Amersham Protran 0.45 µM NC (GE Healthcare)) for one hour at 400mA ...
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bioRxiv - Immunology 2020Quote: ... and then purified using the ÄKTA start protein purification system (GE Life Sciences # 29022094). The supernatant was diluted 1:1 with 20 mM sodium phosphate pH 7.4 and then purified using HisTrap Excel columns (GE Life Sciences # 17-3712-05 ...
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bioRxiv - Microbiology 2019Quote: ... Protein samples were applied to a HiLoad 16/600 Superdex 200 column (GE healthcare) at 1 ml/min pre-equilibrated with dH2O and buffer I (25 mM HEPES/NaOH ...
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bioRxiv - Microbiology 2019Quote: ... Protein sample was applied to a HiLoad 16/600 Superdex 200 column (GE healthcare) at 1 ml/min pre-equilibrated with dH2O and buffer I (25 mM HEPES/NaOH ...
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Cdc42 promotes epithelial morphogenesis by coupling Par-complex and Crumbs recruitment via Par6-aPKCbioRxiv - Cell Biology 2019Quote: ... GST-fusion proteins were purified using Glutathione Sepharose 4 Fast Flow beads (GE Healthcare), washed in lysis buffer and kept on beads in lysis buffer with 1 mM DTT at 4 °C ...
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bioRxiv - Biophysics 2019Quote: ... The eluted protein was concentrated and desalted in a PD-10 column (GE Healthcare) against a Tris 50 mM ...
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bioRxiv - Microbiology 2020Quote: ... Filtered lysate was used for protein purification with an Äkta start system (GE Healthcare). Lysis buffer containing 20 mM imidazole and wash buffer with 40 mM imidazole was used ...
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bioRxiv - Developmental Biology 2019Quote: ... The total lysate was pre-cleared with equilibrated Protein A Sepharose beads (GE Healthcare) for 2 hours at 4 degrees ...
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bioRxiv - Plant Biology 2019Quote: ... Proteins were resolved by SDS-PAGE and transferred to a nitrocellulose membrane (GE Healthcare). StrepII-tagged proteins were detected using Strep-Tactin AP conjugate (IBA ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were visualized with the Amersham ECL Prime kit (GE Healthcare, Little Chalfont, UK). For membrane reprobing ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were visualized via chemiluminescence using the Amersham Imager 600 (GE Healthcare Life Science). Quantification was performed using Aida Image Analyzer v4.26 (Raytest) ...