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Citations for GE Life Sciences :
1401 - 1450 of 4264 citations for Recombinant Human S100A12 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The protein was purified from the lysate using Glutathione-sepharose 4B (GE Healthcare). His-tagged protein expression was induced by shifting to 15°C and adding 0.1 mM IPTG ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was injected into a Superdex 200 column (16/600; GE Healthcare) and eluted as a tetramer with the following buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were transferred onto 0.2 μm pore size nitrocellulose membranes (GE Healthcare, 10600001) at 18 V for 1 h using Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... All the His-tagged proteins were bound to HisTrap HP columns (GE Healthcare) and were eluted with 100 mM-500 mM Imidazole gradients ...
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bioRxiv - Biochemistry 2023Quote: ... All the GST-tagged proteins were bound to GSTrap HP columns (GE Healthcare) and were eluted with 10 mM reduced glutathione ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was then applied to a HiTrap Heparin HP column (GE Healthcare) which was then washed with 10 CV of Heparin Bind Buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... Eluted protein was subjected to a desalting column (PD-10 Columns, GE Healthcare) to remove biotin ...
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bioRxiv - Biophysics 2023Quote: ... the proteins were loaded onto a 1ml HiTrap Q HP column (GE Healthcare). Capping protein heterodimers were separated from other minor protein contaminants by linear gradient elution ...
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bioRxiv - Biochemistry 2023Quote: ... All chromatography steps were done in Fast Protein Liquid Chromatography systems (GE Healthcare).
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bioRxiv - Plant Biology 2023Quote: ... coli Rosetta/DE3 and the proteins purified using glutathione resin (GE Healthcare, USA) as described (Fliegmann et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Pol II was bound to protein G Sepharose Fast Flow beads (GE Healthcare) containing 8WG16 antibody against Rpb1 ...
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bioRxiv - Cell Biology 2023Quote: ... and the rest was incubated with Protein G Mag Sepharose® (GE healthcare), previously coupled to Mis18α antibody ...
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bioRxiv - Microbiology 2023Quote: ... The antibodies were cross-linked to Protein G Mag Sepharose Xtra (GE Healthcare) according to the manufacturer’s instructions before immunoprecipitations ...
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bioRxiv - Synthetic Biology 2023Quote: ... Further protein cleanup and tryptic digest were performed with SP3 beads (GE Healthcare), using the SP3 protocol described in reference (5) ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were then transferred to Cytiva Amersham Hybond PVDF membranes (formerly GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was further purified on a Superdex 200 column (GE Healthcare) containing 50 mM TRIS ...
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bioRxiv - Biophysics 2023Quote: ... The proteins were further purified by HiTrap Heparin HP 5ml column (GE Healthcare) and size exclusion column (Superose 6 Increase 10/300 GL ...
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bioRxiv - Genetics 2023Quote: ... The cleaved protein was concentrated and applied to a Superdex 75 (GE Healthcare) pre-equilibrated with 20 mM Tris-HCl pH 7.8 and 150 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... in a fast protein liquid chromatography (FPLC) system (ÄKTA; GE Healthcare Life Sciences). All chromatographic steps were performed at 4°C ...
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bioRxiv - Microbiology 2023Quote: Protein samples were loaded onto a HiTrap TALON column (5 mL, GE Healthcare) connected to an AKTA prime system (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein was further purified by loading it on a HeparinTrap (GE Healthcare) and eluted with a gradient from 100 mM to 1 M NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... blotted proteins were visualized with an ECLTM Prime Western Blotting System (GE Healthcare). All expression levels were normalized to β-actin expression.
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bioRxiv - Developmental Biology 2024Quote: ... protein samples were precipitated onto Sera-Mag SpeedBeads (GE Healthcare #45152105050250 and #65152105050250) in the presence of 50% EtOH and 2.5% formic acid (FA ...
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bioRxiv - Microbiology 2024Quote: ... The protein was then loaded onto a HiTrap Q HP Column (GE Healthcare) and eluted using a gradient from buffer QA (50 mM Tris ...
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bioRxiv - Plant Biology 2024Quote: ... After the proteins were purified by Glutathione Sepharose 4 Fast Flow (GE Healthcare) and Amylose Resin (New England Biolabs ...
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bioRxiv - Immunology 2024Quote: ... Biotinylated proteins were immobilized on a CAP chip (Biotin CAPture kit, GE Healthcare). As a blocking control anti-LILRB2 antibody ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were detected and measured using the LAS-4000 image analyzer (GE Healthcare) in SYBR–green fluorescence detection mode and Image Quant TL software (GE Healthcare) ...
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bioRxiv - Biochemistry 2024Quote: ... The proteins were purified using Ni Sepharose TM 6 Fast Flow (GE Healthcare).
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bioRxiv - Evolutionary Biology 2024Quote: ... anKaiABC and kaiABC proteins were purified with a GSTrap column (GE Healthcare, USA), Ni-NTA column (Qiagen ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were detected by enhanced chemiluminescence (ECL western blotting detection system, GE Healthcare) using the ImageQuant LAS 4000 system (GE Healthcare).
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bioRxiv - Bioengineering 2020Quote: ... Human peripheral blood mononuclear cells (PBMC) were isolated from healthy volunteer donors by layering over Ficoll-Paque Plus (GE Healthcare) as per the manufacturer’s instructions and were enriched for CD4+ or CD8+ T cells using RosetteSep (STEMCELL Technologies ...
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bioRxiv - Microbiology 2020Quote: Human PBMCs were isolated from leukapheresis obtained from the Central Blood Bank (Pittsburgh, PA) using Ficoll-Paque Plus (GE Healthcare) density gradient centrifugation ...
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bioRxiv - Immunology 2021Quote: ... Human IgGs were isolated from heat-inactivated plasma samples using 5-mL HiTrap MabSelect SuRe columns (GE Healthcare Life Sciences) as described (24).
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bioRxiv - Cell Biology 2022Quote: Fibronectin was purified from outdated human plasma (American Red Cross, Rochester, NY) using gelatin-Sepharose (GE Life Sciences, now Cytiva) affinity chromatography (102) ...
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bioRxiv - Microbiology 2021Quote: ... A549 and human colon carcinoma LoVo cells (both purchased from ATCC) were cultured in F12 Kaighns Modification media (GE Healthcare) with 10% FBS and 1% penicillin/streptomycin.
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bioRxiv - Immunology 2021Quote: ... Human peripheral blood mononuclear cells were isolated from fresh whole blood by Ficoll-Paque (GE Healthcare, Little Chalfont, United Kingdom). CD4+CD45RA+ naïve T cells were isolated using negative enrichment kits (Stemcell Technologies ...
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bioRxiv - Immunology 2021Quote: ... Human and mouse GSDMD were further purified by gel filtration chromatography using a Superdex200 (16/60 GL) column (GE Healthcare) in a running buffer containing 20 mM Tris ...
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bioRxiv - Cancer Biology 2022Quote: ... Human MSLN (Advance BioMatrix 5123-0.1mg) was (10 mg/mL) was immobilized onto a CM5 sensor chip (GE Healthcare, BR100012) by amine coupling ...
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bioRxiv - Molecular Biology 2023Quote: ... monocyte or a macrophage were isolated directly from human whole blood using Ficoll-Paque (Premium, GE Healthcare, Chicago, IL, USA) density gradient centrifugation ...
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bioRxiv - Immunology 2023Quote: ... Human peripheral blood mononuclear cells were isolated from screened buffy coats by density centrifugation with Ficoll-Plaque Plus (GE Healthcare) followed by magnetic-assisted cell sorting ...
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bioRxiv - Plant Biology 2020Quote: ... Secreted proteins were purified from the supernatant by sequential Ni2+ (HisTrap excel; GE Healthcare; equilibrated in 25 mM KPi pH 7.8 ...
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bioRxiv - Biophysics 2020Quote: ... The filtrate was analysed by fast protein liquid chromatography (AKTApurifier, GE Healthcare Life Sciences) using a MonoQ column (5/50 GL ...
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bioRxiv - Biophysics 2020Quote: ... the protein was separated on a RESOURCE™ reversed phase chromatography column (GE Healthcare) using a 0-70 % acetonitrile gradient ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were loaded onto an Äkta Pure chromatography setup (GE Healthcare, Uppsala, Sweden) equipped with a Superdex 200 26/60 column or Superdex 200 16/60 column (GE Healthcare ...
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bioRxiv - Biophysics 2019Quote: ... the protein was further purified using ion-exchange Hi-Trap Q HP-(GE Healthcare). The protein quality was assessed by SDS-PAGE and analytical size-exclusion chromatography using the Superpose 10/300 column (GE Healthcare).
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bioRxiv - Cancer Biology 2021Quote: ... was bound to 20 μL (bed volume) FastFlow Protein A Sepharose beads (GE Healthcare) by incubating for 1 hour at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... and the GST-fusion protein was purified using Glutathione-Sepharose affinity columns (GE Healthcare). The proteins were finally eluted in PBS containing 20 mM glutathione (pH 8 ...
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bioRxiv - Cell Biology 2020Quote: ... Protein was concentrated by centrifugal ultrafiltration and applied to a Superdex-200 (GE Healthcare) size exclusion chromatography column ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations were determined using the 2-D Quant kit (GE Life Sciences 80648356). Samples were reduced (5 mM DTT ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were detected by enhanced chemiluminescence (ECL Prime Western blotting detection reagent; GE Healthcare).