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Citations for GE Life Sciences :
1451 - 1500 of 1534 citations for Anti SDHAF2 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The blots were processed as horizontal cut strips in the respective antibody dilutions and imaged as individual strips in ImageQuant LAS4000 (GE Healthcare, now Cytiva, USA).
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bioRxiv - Molecular Biology 2021Quote: ... 300 µg of the prepared lysate was incubated with the appropriate antibody (3 µg) and pulled down using protein A Sepharose 6MB (GE healthcare # 17-0469-01). All primary antibodies used in this study are listed in table T3 ...
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bioRxiv - Cell Biology 2019Quote: ... using a HiTrap NHS-Activated HP affinity column coupled with aIL-4 monoclonal antibodies and an ÄKTA Fast Protein Liquid Chromatography (FPLC) instrument (GE Healthcare, Piscataway, NJ, USA). Immunizations ...
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bioRxiv - Cell Biology 2020Quote: ... The signal of the secondary antibody was detected by using the detect solution mixture (1:1) (ECL™ Prime Western Blotting System, GE Healthcare Life Science) and a luminescent detector (Amersham Imager 600 ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies were HRP-conjugated and bands were visualized by the ECL Western Blotting Detection Reagent (GE Healthcare, IL, USA Catalog number: 95038–560) and bands were quantified with Gel Doc system (Bio Rad ...
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bioRxiv - Immunology 2021Quote: ... and the homogeneity and the size of the antibodies were analyzed by using an analytical Superdex 200 10/300 column (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Cancer Biology 2019Quote: ... for 1 h incubated with the appropriate antibodies and then visualized using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Cell Biology 2021Quote: ... For co-immunoprecipitation of endogenous SQSTM1/p62 the cell lysates were incubated for 2-4 h with specific antibody followed by 1-2 h with protein-G coupled Sepharose beads (GE Healthcare, 17-0885-01). To resolve protein complexes for denaturing immunoprecipitation ...
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bioRxiv - Immunology 2020Quote: ... and the size and purity of each antibody were determined by size exclusion chromatography using a Superdex 200 10/300 column (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were washed three times with 0.1% PBS-T and were incubated with secondary horseradish peroxidase-coupled antibodies for 1h at RT (GE Healthcare, Jackson ImmunoResearch, 1:10000). ECL Western Blotting Detection Reagent (RPN2106 ...
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bioRxiv - Biophysics 2019Quote: ... supernatant was incubated with anti-GFP nanobodies immobilized on CNBr-activated sepharose resin (GE Healthcare, 17-0430-01) at a ratio of 1 mL resin per 10 g of cells and gently rotated on an orbital rocker for 3 hours ...
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bioRxiv - Molecular Biology 2022Quote: rhGAA secreted from D534 was also purified using 1 mL of M2 anti-FLAG affinity column (GE healthcare) according to the manufacturer’s instruction ...
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bioRxiv - Genomics 2022Quote: ... with an anti-rabbit IgG (goat polyclonal, 1:10,000 in 3% BSA/TBS-T; GE Healthcare, #RPN4301, RRID:AB_2650489) secondary antibody was used as a loading control ...
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bioRxiv - Physiology 2023Quote: ... and subsequently probed with secondary anti-mouse/-rabbit IgG (H+L)-HRP conjugates (1:6000, GE Healthcare, USA). Quantification was carried out on original scanned images using Image Lab software (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... Western blotting was performed as previously described using Horseradish Peroxidase (HRP)-conjugated secondary antibody followed by addition of ECL (GE Healthcare Bio-Sciences Corp., USA) to reveal chemiluminescence71 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Membranes were washed and incubated with the appropriate horseradish peroxidase-conjugated secondary antibody for 1 h at room temperature and detected by chemiluminescence system (GE Healthcare Europe GmbH, Freiburg, Germany).
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bioRxiv - Immunology 2019Quote: ... IgG and IgM antibodies were purified from plasma samples by affinity chromatography using prepacked HiTrap™ Protein G HP columns (GE Healthcare, Wauwatosa, WI, USA) and HiTrap™ IgM Purification HP columns (GE Healthcare ...
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bioRxiv - Physiology 2020Quote: ... Immunoblotting was conducted with specific antibodies and protein bands were visualized using the enhanced chemiluminescence (ECL) Western Blotting Detection System (GE Healthcare, Chalfont St Giles, UK) or the Odyssey infrared detection system (LICOR).
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bioRxiv - Microbiology 2020Quote: ... IgG and IgA antibodies were depleted from plasma obtained from 25 recovered COVID-19 patient using Protein G HP Spintrap (GE Healthcare Life Sciences, Buckinghamshire, UK) and Peptide M / Agarose (InvivoGen ...
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bioRxiv - Immunology 2020Quote: ... and class II molecules were isolated by standard immunoaffinity purification using purified monoclonal antibody 2G11 [65] produced in-house and covalently linked to CNBr-activated Sepharose (GE Healthcare, Chalfont St Giles, UK). MHC–peptide complexes were eluted by repeated addition of 0.2% trifluoroacetic acid (TFA ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by an incubation with horseradish peroxidase (HRP) secondary antibodies (Mouse IgG HRP Linked Whole Ab-GE Healthcare, #NA931, 1:5000; Rabbit IgG HRP Linked Whole Ab-GE Healthcare, #NA934, 1:5000) in 3% milk in TBST ...
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bioRxiv - Cancer Biology 2022Quote: 1 mg lysate protein aliquots were used for immunoprecipitation with optimized amounts of the indicated antibodies and 20 μL of Protein A Sepharose beads (GE Healthcare, Chalfont St. Giles, UK) followed by SDS-Polyacrylamide (Biorad ...
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bioRxiv - Pathology 2020Quote: ... Membranes were blocked and incubated with the antibody to detect neural Wiskott-Aldrich Syndrome protein (N-WASp; MilliporeSigma, Burlington, MA, USA) using horseradish peroxidase-linked secondary antibodies and an Enhanced Chemiluminescence System (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA). Autoradiography was performed using UltraCruz Autoradiography Films (Santa Cruz Biotechnology) ...
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bioRxiv - Neuroscience 2021Quote: ... Immunoreactivity was detected using a horseradish peroxidase (HRP)-conjugated anti-rabbit immunoglobulin G (IgG, 1:10,000, NA934 GE Healthcare). Tau13 and Tau5 immunoreactivity were detected using an anti-mouse IgG (1:10,000 ...
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bioRxiv - Microbiology 2019Quote: ... and RbmAb-phospho-histone H3 (Ser10/Thr11) were detected with donkey anti-rabbit IgG conjugated to HRP (GE Healthcare).
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bioRxiv - Immunology 2021Quote: Kinetics binding measurement for anti-spike mAbs in this study was performed using a Biacore T200 instrument (GE Healthcare). The system was flushed with filtered 1xHBS-P+ running buffer (0.01M HEPES ...
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bioRxiv - Genetics 2023Quote: ... Blots were washed 3 times with 1x TBS-T and incubated with anti-mouse HRP (GE Healthcare Cat # NXA931) or anti-rabbit HRP secondary antibody (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... washed in TBST and detected with anti-Mouse IgG-Horseradish peroxidase (GE Healthcare Life Science, Cat# NA931, 1:5000). For streptavidin staining ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed three times for 10 min in TBS-T and incubated with the secondary horse radish peroxidase (HRP) coupled antibody (GE Healthcare, NXA931V; in a 1:3000 dilution) for two hours under agitation at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... The following antibodies have been conjugated to fluorochromes using commercially available kits according to the manufacturer’s instructions: anti-S100B has been conjugated to FITC (FLUKA) using Illustra NAP-5 columns (GE Healthcare), anti-GD2 (ch14:18 ...
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bioRxiv - Cell Biology 2020Quote: ... The membranes were then probed with HRP-conjugated anti-mouse IgG (Jackson Immunochemicals; 1:10,000) and binding was detected using ECL (GE Healthcare) in a LAS4000 instrument.
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bioRxiv - Genomics 2020Quote: ... The membrane was incubated with 1:10,000 dilution of anti-rabbit IgG conjugated to horse radish peroxidase (HRP; GE Healthcare) diluted in 2.5% skimmed milk in TBS-T or anti-mouse IgG conjugated to HRP (GE Healthcare ...
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bioRxiv - Genomics 2020Quote: ... Mouse Anti-Rabbit IgG (Light Chain Specific) (CST, #93702S) (1:5000) or Rabbit IgG HRP Linked Whole (GE Healthcare, NA9340), respectively ...
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bioRxiv - Molecular Biology 2019Quote: ... the membrane was incubated with peroxidase-labeled donkey anti-rabbit IgG (#NA9340-1ML, Ge Healthcare Life Sciences, Chicago, IL, USA) with 5% nonfat dry milk for one hr ...
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bioRxiv - Cell Biology 2019Quote: ... Membranes were quickly washed 3x in TBST (TBS and 0.1% Tween 20) and then incubated with HRPO-linked anti-mouse IgG (both GE Healthcare) diluted 1:3,000 in blocking solution for 45 min ...
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bioRxiv - Cell Biology 2019Quote: ... The detection of tubulin using mouse anti-tubulin KMX-1 primary (1:100; Gift from Keith Gull, Oxford) and anti-mouse-HRP secondary (1:20,000, GE Healthcare) was used to verify equal sample loading ...
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bioRxiv - Immunology 2019Quote: ... were captured via their histidine tags onto CM5 chips pre-coupled with ~9000 reflective units anti-His Ab (GE Healthcare) using standard amine chemistry ...
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bioRxiv - Plant Biology 2020Quote: ... the membrane was washed three times with TBS containing 0.1% Tween 20 and incubated with horseradish peroxidase-linked anti-rabbit IgG (NA934, GE Healthcare) at a dilution of 1:10,000 for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... Anti-Fh15 polyclonal IgG was purified by affinity chromatography using 5/5/HiTrap Protein-A HP (GE Healthcare, Piscataway, NJ). The resulting IgG was conjugated with horseradish peroxidase (HRP ...
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Proteolytic cleavage of the extracellular domain affects signaling of parathyroid hormone receptor 1bioRxiv - Pharmacology and Toxicology 2022Quote: ... followed by horseradish peroxidase-conjugated goat anti-rabbit (1:10,000) and detection on Super RX X-ray film (Fujifilm) using ECL Plus reagent (GE Healthcare).
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bioRxiv - Molecular Biology 2019Quote: ... Western blot of the immunoprecipitated recombinant proteins was performed as described in the above section using anti-GST HRP (horseradish peroxidase) conjugate (1:10,000 Amersham GE Healthcare) for 60 minutes at room temperature under agitation ...
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bioRxiv - Physiology 2021Quote: ... The immunostaining was detected using horseradish peroxidase-conjugated anti-rabbit immunoglobulin G (IgG) (GE Healthcare, Buckinghamshire, UK; dilution: 1:5000) or anti-mouse IgG (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... we coupled 130 μg of a mouse monoclonal anti-H4K12Ac (gift from Hiroshi Kimura) to 12.5 μl rProtein A Sepharose (GE Healthcare) slurry in antibody coupling buffer (10 mM K-HEPES pH=8 ...
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bioRxiv - Immunology 2020Quote: ... the membrane was washed with PBS-T and then incubated with HRP-conjugated anti-Rat IgG (1:1000; GE healthcare) for 1 h at RT ...
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bioRxiv - Cell Biology 2019Quote: ... The membranes were then probed with HRP-conjugated anti-mouse IgG (Jackson Immunochemicals; 1:10,000) and binding was detected using ECL (GE Healthcare) in a LAS4000 instrument.
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bioRxiv - Physiology 2024Quote: ... Immune complexes were visualized using horseradish peroxidase-linked goat anti-rabbit immunoglobulin and enhanced chemiluminescence reagents (GE Healthcare, Tokyo, Japan).
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bioRxiv - Microbiology 2023Quote: ... and washed 10 minutes 3 times in PBST before being incubated with horseradish peroxidase-conjugated anti-rabbit IgG (GE Healthcare) diluted 1:2000 in the blocking buffer for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was mixed with pre-equilibrated anti-GFP nanobody (GFPnb)-coupled cyanogen bromide-activated Sepharose beads (GE Healthcare) at 4 °C for 2 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... Anti-Strep blots were imaged using an Amersham Imager 600 and Amersham ECL Prime Western Blotting Detection Reagent (GE Life Sciences).
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A quantitative tri-fluorescent yeast two-hybrid system: from flow cytometry to in-cellula affinitiesbioRxiv - Biochemistry 2019Quote: ... The membrane was then incubated for one hour at room temperature in presence of a sheep anti-mouse whole IgG HRP (GE Healthcare) secondary antibody diluted 1/5000 in blocking buffer ...