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Citations for GE Life Sciences :
1301 - 1350 of 1534 citations for Anti SDHAF2 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... was cloned into mouse IgG1 or human IgG1 Fc backbone in pKN293E expression vectors and transiently transfected into HEK293 cells followed by media collection and purification using MabSelect SuRe antibody purification resin (Cat: 29-0491-04, GE Healthcare). SEC-HPLC and SDS-PAGE were used to check the size and purity of these recombinant proteins.
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bioRxiv - Cell Biology 2020Quote: ... Proteins were detected with secondary antibodies conjugated to horseradish peroxidase (RPN4201 and RPN4301) and the ECL detection kit (both GE Healthcare). Visualization of protein bands was realized by LAS-4000 mini (Fujifilm) ...
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bioRxiv - Cell Biology 2020Quote: ... Antibody-antigen complexes were recovered by incubating for 1 h at 4°C with Protein A-Sepharose CL-4B (GE Healthcare) and eluted with Laemmli sample buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoblots were visualized using a horseradish peroxidase-conjugated secondary antibody followed by enhanced chemiluminescence detection with an ImageQuant LAS 4000 developer (GE Healthcare).
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bioRxiv - Molecular Biology 2021Quote: The binding affinity of the discovered antibodies for α-cobratoxin was determined using Surface Plasmon Resonance (SPR) (BIAcore T100, GE Healthcare). All measurements were performed at 25 °C using 10 mM HEPES ...
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bioRxiv - Microbiology 2022Quote: Kinetic interactions between the antibodies and his-tagged antigen proteins were measured at room temperature using Biacore T200 surface plasmon resonance (GE Healthcare). Anti-human fragment crystallizable region (Fc region ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3 times with PBST and then placed in 5% milk PBST plus 1:2500 rabbit specific horseradish peroxidase (HRP) antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Neuroscience 2021Quote: ... S9.6 antibodies were purified according to standard procedures (Stork et al., 2016) by using a HiTrap Protein G HP column (GE Healthcare), followed by extensive washing with 20 column volumes of PBS and elution with five column volumes of elution buffer (0.1 M glycine-HCl ...
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bioRxiv - Biochemistry 2019Quote: ... and a 1:2500 dilution of the fluorophore-conjugated secondary antibody Cy3 (ECL Plex goat-α-mouse IgG-Cy3, GE Healthcare). Detection was carried out with the Ettan DIGE Imager (GE Healthcare) ...
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bioRxiv - Molecular Biology 2019Quote: ... Antibodies were diluted in Can Get Signal Immunoreaction Enhancer Solution (Toyobo, NKB-101) and detected with ECL Prime (GE Healthcare, #RPN2232).
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bioRxiv - Genetics 2019Quote: ... Following washing with TBST membranes were incubated with ECL horse radish peroxidase (HRP)-conjugated secondary antibodies (GE Healthcare, Chicago, IL, USA) diluted 1:5000 in blocking buffer for one hour at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... The expressed GP was purified using an mAB100 antibody column followed by SEC on a HiLoad Superdex 200 16/600 column (GE Healthcare). PBS (pH 7.2 ...
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bioRxiv - Bioengineering 2020Quote: ... The antibody expression vectors were co-transfected into 293F cells and the cell culture supernatants containing secreted antibody IgG were purified by columns packed in-house containing Protein A Sepharose beads (GE Healthcare).
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bioRxiv - Neuroscience 2020Quote: ... membranes were treated with peroxidase-conjugated secondary antibodies for 2h at room temperature and revealed using a chemiluminescence detection kit (ECL, GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... YL½ antibody (IgG, expressed from hybridoma cells and purified by ion exchange chromatography) was coupled to CNBr-activated sepharose beads (GE Healthcare) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... Anti-NPR1 antibodies were purified from antisera by affinity chromatography using a tNPR1-His-immobilized column (Hitrap NHS-activated HP; GE Healthcare). To prepare for the tNPR1-His-immobilized column ...
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bioRxiv - Systems Biology 2020Quote: ... the target-specific antibody was added to the cellular lysates together with 25 μl of Protein A or G sepharose (GE Healthcare) depending on the species of target antibody and the mixture was incubated overnight rotating at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Background binding to reference flow cells was subtracted and antibody binding levels were calculated using Biacore T200 evaluation software (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... AlexaFluor conjugated secondary antibodies targeting respective primary antibodies (Thermoscientific, USA) were used to visualize red (IdU) and green (CldU) DNA fibers on a Deltavision microscope (Applied Precision) and analyzed using softWoRx 5.5 software ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were incubated with HRP-coupled secondary antibodies (santa cruz, cell signaling) for 1 h at RT and chemiluminescence detected with ECL Prime (GE Healthcare) at the LAS-1000 (Fujifilm) ...
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bioRxiv - Cell Biology 2022Quote: ... After incubation with primary antibodies overnight at 4°C followed by the addition of Protein A or G Sepharose beads (GE Healthcare) for 2-3 hrs ...
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bioRxiv - Immunology 2022Quote: The binding kinetics and affinity of recombinant monoclonal antibodies for the SARS-CoV-2 RBD protein (SinoBiological) were analyzed by SPR (Biacore T200, GE Healthcare). Specifically ...
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bioRxiv - Microbiology 2022Quote: Kinetic interactions between the antibodies and his-tagged antigen proteins were measured at room temperature using Biacore T200 surface plasmon resonance (GE Healthcare). Anti-human fragment crystallizable region (Fc region ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were then washed with TBS-T (3x, 5 min each) and incubated with horse radish peroxidase (HRP)-conjugated secondary antibodies (GE Healthcare). Following another wash step in TBS-T ...
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bioRxiv - Biochemistry 2022Quote: Antibody binding to SARS-CoV-2 spike was assessed using SPR on a Biacore T-200 (Cytiva, MA, formerly GE Healthcare) with HBS buffer supplemented with 3 mM EDTA and 0.05% surfactant P-20 (HBS-EP+ ...
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bioRxiv - Immunology 2020Quote: ... The bound antibodies were eluted and further purified by gel-filtration chromatography using a Superdex 200 High Performance column (GE Healthcare). The purified antibodies were either used in binding and neutralizing assays.
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bioRxiv - Immunology 2020Quote: ... Recombinant IgG antibodies were purified from cell-free supernatants by affinity purification using Protein G or Protein A sepharose beads (GE Healthcare). Purified proteins were dialyzed in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Horseradish peroxidase-conjugated secondary antibodies and enhanced chemiluminescence solution (MoBiTec) were used for visualization on an Amersham Imager 680 (GE Healthcare) or a Cawomat 2000 IR apparatus (CAWO solutions).
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bioRxiv - Cell Biology 2021Quote: ... followed by incubation with horseradish peroxidase-conjugated secondary antibodies (Table S6) and detected with enhanced chemiluminescence reagents (Amersham Bioscience) using Amersham hyperfilm (GE Healthcare) or the G:Box Chemi (Syngene) ...
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bioRxiv - Biochemistry 2021Quote: The binding affinities of full IgG antibodies to SARS-CoV-2 spike protein were determined using surface plasmon resonance (SPR) and a BIAcore T200 instrument (GE Healthcare) at 25°C ...
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bioRxiv - Immunology 2022Quote: ... The antibody and RBD affinity ability were estimated employing a 1:1 binding fit model in Biacore Evaluation software (GE Healthcare).
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bioRxiv - Immunology 2019Quote: ... Membranes were blocked with 5% bovine serum albumin and incubated with the appropriate primary and HRP-conjugated secondary antibodies (GE Healthcare). Western Lightning ECL Pro (PerkinElmer ...
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bioRxiv - Immunology 2020Quote: ... The cell lysates were incubated with rotation at 4°C first for 45 min with antibodies to FLAG and then for 45 min with protein A–Sepharose (GE Healthcare). The resulting immunoprecipitates were subjected to immunoblot analysis.
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bioRxiv - Cell Biology 2020Quote: ... Membranes were probed using the indicated antibodies and chemiluminescent signals was detected by a LAS 4000 mini Image Quant system (GE Healthcare). Band intensity was quantified using ImageJ ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed three times 10 minutes and incubated for at least one hour in HRP-conjugated secondary antibodies (GE Healthcare) at 1:8000 dilution in PBS-T ...
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bioRxiv - Microbiology 2020Quote: ... blocked for 1 hour and immuno-detected using Anti-RecA (E. coli) antibody (1000x dilution) (gift from Mike Cox, (52)) and analyzed using an ECL Prime detection kit (GE Healthcare). Densitometry was performed using ImageJ (https://imagej.nih.gov/ij/)
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bioRxiv - Neuroscience 2020Quote: ... Immunoprecipitation was performed with the indicated primary antibodies and 50 μl of a 50% slurry of protein-A sepharose beads (GE Healthcare) for 2 h at 4°C ...
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bioRxiv - Physiology 2020Quote: ... Blots were then washed in TBST and incubated for 1 h at 22-24°C with secondary HRP-conjugated antibodies (GE Healthcare) diluted 5000-fold in 5% skim milk in TBST ...
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bioRxiv - Cell Biology 2019Quote: ... the antibodies were separated from unconjugated dye by gravity filtration through Sephadex G-25 DNA grade size exclusion columns (GE Healthcare) by visual detection ...
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bioRxiv - Cell Biology 2020Quote: ... antigen was CENP-AHCP-3(105-183)::6xHis) and detected using an HRP-conjugated secondary antibody (rabbit or mouse; GE Healthcare). For antibody production CENP-AHCP-3(105-183)::6xHis was expressed in E ...
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bioRxiv - Immunology 2021Quote: ... All antibody formats were expressed using ExpiCHOTM Expression system (Thermoscientific, USA) and purified on HiTrap Protein-A column (GE healthcare, UK). The integrity and purity of the antibodies were analyzed using SDS-PAGE.
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bioRxiv - Immunology 2021Quote: The binding affinities of antibodies to SARS-CoV-2 spike protein were determined using surface plasmon resonance (SPR) and a BIAcore T200 instrument (GE Healthcare) at 25°C ...
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bioRxiv - Immunology 2020Quote: ... the wells were washed with PBS containing 0.05 % Tween®20 (PBS-T) and incubated with HRP-conjugated antibodies (GE healthcare) for 3 hours at RT ...
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bioRxiv - Genetics 2020Quote: 200 µg of antibodies R1 and R2 (against HSF2BP and MIDAP) were crosslinked to 100 ul of sepharose beads slurry (GE Healthcare). Testis extracts were prepared in 50mM Tris-HCl (pH8) ...
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bioRxiv - Molecular Biology 2021Quote: ... which were supplemented with KCl to a final concentration of 100 mM were subjected directly to immunoprecipitation and incubated for overnight at 4 °C with antibodies and protein G-Sepharose 4 Fast Flow (GE Healthcare) or HA magnetic beads (Pierce) ...
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bioRxiv - Microbiology 2022Quote: ... Antibody supernatant was harvested 6 days following transfection and purified using protein G affinity chromatography following the manufacturers protocol (GE Healthcare). Specificity of the cloned mAb was determined by ELISA.
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bioRxiv - Developmental Biology 2022Quote: ... Secondary antibodies coupled to horseradish peroxidase were used for enhanced chemiluminescence signal detection with ECL Prime Western Blotting System (GE Healthcare).
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bioRxiv - Cancer Biology 2022Quote: ... were resuspended in 500 μl IP buffer and incubated with the appropriate antibodies on ice for 2 h and then 30 μl protein-G-sepharose beads (GE Healthcare; equilibrated once in 10 ml water and three times in washing buffer ...
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bioRxiv - Microbiology 2022Quote: ... and bands visualized with (HRP)- coupled secondary antibody (1:5,000) (Cell Signalling, rabbit 7074S, mouse 7076S) on an ImageQuant LAS 4000 Mini (GE Life Sciences) with Luminata Forte Western HRP Substrate (Sigma WBLUF0500 ...
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bioRxiv - Immunology 2022Quote: ... RPAPGST(GalNAc)APPAHG and RPAPGS(GalNAc)T(GalNAc)APPAHG with immobilized antibody 14A and 16A were determined by surface plasmon resonance on a Biacore T200 (GE Healthcare) instrument ...