Labshake search
Citations for GE Life Sciences :
1451 - 1500 of 3197 citations for 6 Bromo 2 trifluoromethylimidazo 1 2 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... The eluted Cavin4a-FL and Cavin4b-FL proteins were loaded on size exclusion chromatography column Superose™ 6 Increase 10/300 GL column (GE healthcare) pre-equilibrated with 0.22 μm filtered 50 mM HEPES ...
-
bioRxiv - Microbiology 2020Quote: ... The resulting pellet was resuspended in TET and complexes were further separated by Superose 6 10/300 column chromatography in TET buffer with 150 mM NaCl using an AKTA Pure system (GE Life Sciences). The sample collected from the column was used for microscopy and visualized by SDS-PAGE with silver staining (ProteoSilver™ Plus Silver Stain Kit) ...
-
bioRxiv - Biochemistry 2021Quote: ... Cleaved topo VI fractions were pooled and concentrated using Amicon Ultra 15 mL centrifugal filter units (30 kDa cut off, MilliporeSigma) before being passed down a Superose 6 10/300 (GE Life Sciences) column in Buffer C (20 mM HEPES pH 7.5 ...
-
bioRxiv - Microbiology 2021Quote: ... The resulting pellet was resuspended in TET and complexes were further separated by Superose 6 10/300 column chromatography in TET buffer with 150 mM NaCl using an AKTA Pure system (GE Life Sciences). The sample collected from the column was used for microscopy ...
-
bioRxiv - Cancer Biology 2022Quote: ... a selection of mice were injected intra-peritoneally with 3 rounds of 6 injections each of 200μg poly(I:C) (GE Healthcare, #27-4732-01). Poly(I:C ...
-
bioRxiv - Biochemistry 2019Quote: ... the reaction was quenched with 10 mM DTT and subjected to size-exclusion chromatography on a Superose™ 6 Increase 10/300 (GE Healthcare) in GF buffer ...
-
bioRxiv - Molecular Biology 2019Quote: ... The reaction was stopped with 1.7 mM Tris-HCl pH 8.0 and crosslinked TcdA1-BSA-Lewis X was purified from excess of BSA-Lewis X by SEC using a Superose 6 3.2-300 column (GE Healthcare Life Sciences).
-
bioRxiv - Genomics 2021Quote: ... Barcoded beads were resuspended in lysis buffer (200 mM Tris-HCl pH8.0, 20 mM EDTA, 6% Ficoll PM-400 (GE Healthcare/Fisher Scientific), 0.2% Sarkosyl (Sigma-Aldrich) ...
-
bioRxiv - Biochemistry 2020Quote: ... The dried butanol fraction was then redissolved in 20mM of sodium phosphate buffer (pH 6) and desalted using a NAP10 desalting column prepacked with Sephadex G-25 resin (GE healthcare, USA). Finally ...
-
bioRxiv - Microbiology 2020Quote: ... The protein (5-6 ml) was further purified by FPLC over a 26/60 Superdex 200 pg size exclusion column (GE Life Sciences) equilibrated with 20 mM Tris (pH 8.0 ...
-
bioRxiv - Immunology 2020Quote: ... Complex samples were either diluted to 0.02 mg/mL and applied to glow discharged negative stain grids or they were SEC purified using a Superose™ 6 Increase 10/300 GL (GE Healthcare) column to remove excess Fab prior to EM grid preparation ...
-
bioRxiv - Genetics 2020Quote: ... the eluted protein solution was concentrated 10 times in a Vivaspin concentrator 30 kDa cut off and loaded in a Superose 6 increase 10/300 (GE Life Sciences) with PBS as a carrier buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... filtered (0.22 mm) and then applied to 5 mL HiTrap Chelating HP columns (GE Healthcare, protein from 6 L cells per column) charged with Ni2+ and equilibrated with 4 column volumes of buffer C (20 mM Tris-HCl ...
-
bioRxiv - Plant Biology 2021Quote: Secreted candidate proteins were purified by immobilized metal ion affinity (IMAC) using Ni Sepharose 6 Fast Flow (GE Healthcare, Chicago, IL, EUA) according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2021Quote: ... The eluent was concentrated and further purified by using a size-exclusion chromatography (SEC) column (Superose 6 increase 10/300 GL, GE Healthcare, USA) in 50 mM Tris-HCl (pH 8.0) ...
-
bioRxiv - Biochemistry 2021Quote: ... The samples (equivalent to ∼85 µg microsomes) were then analyzed with an HPLC system and injected to a Superose 6 column (GE Life Sciences), equilibrated in a running buffer containing 25 mM Tris pH 7.5 ...
-
bioRxiv - Immunology 2021Quote: The protein was purified from filtered cell supernatants using Strep-Tactin resin (IBA) before being subjected to additional purification by gel filtration chromatography using a Superose 6 10/300 column (GE Healthcare, USA) in 1 × PBS ...
-
bioRxiv - Biophysics 2022Quote: ... Sample homogeneity was investigated by analytical size exclusion chromatography on a Superose® 6 10/300 GL gel filtration column (GE Healthcare). Aliquots of purified EFL at 0.4 mg/mL were flash frozen in liquid nitrogen and stored at −70 °C.
-
bioRxiv - Microbiology 2022Quote: ... The leukocyte-depleted red blood cell pellet was then resuspended in 10 ml PBS and 5 ml were carefully layered on 6 ml of 70% (v/v) isotonic Percoll cushion (GE Healthcare, UK). After centrifugation at 1200 x g for 15 mins ...
-
bioRxiv - Biochemistry 2024Quote: ... The resulting ABC holotoxins were then separated from excess TcB-TcC by size exclusion chromatography on a Superose 6 increase 5/150 column (GE Life Sciences) equilibrated in labelling buffer ...
-
bioRxiv - Molecular Biology 2024Quote: SEC-MALS experiments were performed using and Ä KTA purifier system connected to a Superose 6 Increase 10/300 GL column (GE healthcare) at a flow rate of 0.4 ml/min in running buffer (100 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Genetics 2024Quote: ... Barcoded beads were resuspended in lysis buffer (200 mM Tris-HCl pH8.0, 20 mM EDTA, 6% Ficoll PM-400 (GE Healthcare/Fisher Scientific), 0.2% Sarkosyl (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2024Quote: Purified LZTR1 proteins were centrifuged at 12,000×g for 10 min before being applied to an analytical Superose 6 10/300 SEC column (GE Healthcare Life Sciences) using a buffer containing 50 mM Tris/HCl (pH 7.4) ...
-
bioRxiv - Genetics 2023Quote: ... GST-Clr4 and MBP-Clr6 were expressed in Escherichia coli BL21 (DE3) cells and purified on Ni+ SepharoseTM 6 Fast Flow (for 6His fusions; GE Healthcare, 17531806), Glutathione SepharoseTM 4B (for GST fusions ...
-
bioRxiv - Biophysics 2022Quote: ... The concentrated protein sample was subjected to further size-exclusion chromatography (SEC) by a Superose 6 Increase 10/300 GL gel filtration column (GE Healthcare, USA) using a flow rate of 0.3 mL/min and a running buffer containing 20 mM HEPES pH 7.5 ...
-
bioRxiv - Biochemistry 2023Quote: ... Transfected cells were harvested 72 hours post-transfection and the 6 × His-tagged protein in the supernatant was purified using Ni-NTA (GE Healthcare, USA) affinity chromatography ...
-
bioRxiv - Physiology 2023Quote: Plasma samples (200 μL) were loaded onto AKTA pure FPLC column [Superose™ 6 Increase 10/300 GL FPLC column (GE Healthcare). Lipoproteins were eluted with PBS and 0.25 mL fractions were collected at a flow rate of 0.4 mL/min.
-
bioRxiv - Biochemistry 2024Quote: Oligomerization of ClpL WT and mutants was investigated by size exclusion chromatography (SEC) using a Superose® 6 10/300 GL column (GE Healthcare) at 4°C in assay buffer supplemented with 2 mM ATP ...
-
bioRxiv - Biophysics 2020Quote: ... The flow-through was then collected and concentrated using a 100 kDa concentrator and further purified on a Superose 6 gel filtration column (GE Healthcare Life Sciences) in 20 mM Tris pH 8.0 ...
-
bioRxiv - Plant Biology 2020Quote: Protein extracts (150 mg per sample) were incubated with 300 µl of IgG-Sepharose 6 Fast Flow beads (GE Healthcare, Chicago, IL, USA) for 2 h at 4°C with gentle rotation (1000 rpm) ...
-
bioRxiv - Plant Biology 2020Quote: ... Obtained samples were fractionated on the column Superose 6 10/300 GL (50 kDa - 5 MDa range, GE Healthcare Life Sciences, Äkta FPLC) at the rate 0.5 ml/min in 20 mM Tris-HCl pH7.4 and 150 mM NaCl ...
-
bioRxiv - Biochemistry 2020Quote: ... The supernatant was injected into a Ni2+-NTA agarose affinity column (containing 5 mL Ni2+-Sepharose 6 FF beads, GE Healthcare, Cat. 17531803). The column was incubated for 15 minutes and washed twice with 30 mL washing buffer (50 mM Tris ...
-
bioRxiv - Microbiology 2020Quote: ... This was performed using an purifier system connected to a Superose 6 Increase 10/300 GL column (both from GE healthcare, Solingen, Germany) at a flow rate of 0.5 ml · min−1 in running buffer (50 mM Tris/HCl (pH 8.0) ...
-
bioRxiv - Cell Biology 2021Quote: ... The supernatant was collected and then incubated with 500-μl of Nickel-Sepharose 6 Fast Flow (GE Healthcare Life Sciences, Piscataway, NJ, USA) for 2 h at 4°C ...
-
C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: Gel filtration was performed using fast protein liquid chromatography (AKTA-FPLC system) on a Superose 6 10/300 GL column (GE Healthcare Life Sciences) as described previously (Hořejší et al. ...
-
bioRxiv - Biochemistry 2022Quote: ... Mpro was eluted with 300 mM imidazole in the lysis buffer and then purified by size-exclusion chromatography using a pre-packed Superose 6 Increase 10/300 GL column (GE Healthcare Life Sciences) equilibrated in 50 mM Tris pH 8.0 ...
-
bioRxiv - Biochemistry 2022Quote: ... The protein sample was then purified by size-exclusion chromatography using a pre-packed Superose 6 Increase 10/300 GL (GE Healthcare Life Sciences) equilibrated in 20 mM HEPES pH 7.0 ...
-
bioRxiv - Biochemistry 2022Quote: ... The protein sample was then purified by size- exclusion chromatography using a pre-packed Superose 6 Increase 10/300 GL (GE Healthcare Life Sciences) equilibrated in 50 mM Tris pH 8.0 ...
-
bioRxiv - Microbiology 2020Quote: ... according to manufacturer’s protocol and removed the FLAG tag and His6-tag with TEV digestion followed by reverse nickel affinity purification and sizeexclusion chromatography on a Superose 6 Increase column (GE Healthcare Life Sciences). We biotinylated the protein with BirA ligase as previously described32.
-
bioRxiv - Immunology 2022Quote: ... The spike proteins were purified from the supernatants on complete™ His-Tag Purification Resin before further purification with Superose 6 increase (S6i) 10/300 column (GE Healthcare Biosciences). The H1 HA gene derived from A/Michigan/045/15 H1 was cloned into pcDNA3.4 vector and contains a GCN4 trimerization domain (folded ...
-
bioRxiv - Microbiology 2020Quote: ... The sdAbs with a His-tag fused at C-terminus were purified over Ni Sepharose 6 Fast Flow (GE Healthcare, Boston, MA, USA) and eluted with 400 mM imidazole ...
-
bioRxiv - Biochemistry 2019Quote: ... were mixed together and incubated for at least 1 h at 4 °C before removing the excess of free TcB-TcC by SEC on a Superose 6 Increase 5-150 column (GE Healthcare Life Sciences). For TcB-TcC variants containing HVR-RBD chimeras ...
-
bioRxiv - Immunology 2020Quote: Purified C105 Fab was incubated with SARS-CoV-2 S trimer at a 2:1 molar ratio per protomer on ice for 30 minutes prior to purification by SEC on a Superose 6 Increase 10/300 GL column (GE Healthcare Life Sciences). Fab–S complexes were concentrated to 1.6 mg/ml in Tris-buffered saline (TBS) ...
-
bioRxiv - Biochemistry 2022Quote: ... SEC-HPLC was carried out using an Alliance e2695 HPLC system (Waters Co., Milford, MA, USA) equipped with a Superose 6 Increase 10/300 GL column (GE Healthcare Life Sciences) and a 2998 photodiode array (PDA ...
-
bioRxiv - Biochemistry 2024Quote: ... samples were loaded onto a Superose 6 10/300 gel filtration column under the control of an ÄKTA Pure instrument (GE Healthcare Life Sciences). Proteins were eluted using 50 mM HEPES ...
-
bioRxiv - Biochemistry 2024Quote: ... The ultracentrifuged sample was purified by size-exclusion chromatography on a Superose™ 6 Increase 10/300 GL column (GE Healthcare Life Sciences), equilibrated with buffer containing 20CmM Tris-HCl ...
-
bioRxiv - Biophysics 2023Quote: ... The digested protein was concentrated using a 100 kDa concentrator and further purified on a Superose 6 Increase 10/300 gel filtration column (GE Healthcare Life Sciences) equilibrated in an Mg2+-containing buffer (20 mM Tris pH 8.0 ...
-
bioRxiv - Biophysics 2023Quote: ... Free biotin was removed using size-exclusion chromatography by loading the labeled protein on a Superose 6 gel-filtration column (GE Healthcare, Pittsburgh, PA) eluted with 20 mM HEPES pH 7.5 ...
-
bioRxiv - Biochemistry 2023Quote: ... Free biotin (or dye) was removed using size-exclusion chromatography by loading the labelled protein on a Superose 6 gel filtration column (GE Healthcare, Pittsburgh, PA) eluted with 20 mM HEPES pH 7.5 ...
-
bioRxiv - Immunology 2023Quote: ... The EROS-NOX2-p22phox-7G5 Fab complex was concentrated and applied onto a Superose 6 Increase 10/300 GL column (GE HealthCare, Chicago, IL) in Buffer D (20 mM Tris pH 8.0 ...