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Citations for GE Life Sciences :
101 - 150 of 754 citations for SARS Coronavirus Spike Glycoprotein S2 Mosaic C Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... coli cell fluorescence was carried out using a Typhoon FLA 9500 fluorimager (GE Healthcare) as described in Olorunniji et al ...
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bioRxiv - Biochemistry 2024Quote: ... coli as His-tag proteins and were purified using the IMAC principle (GE healthcare). Following purification ...
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bioRxiv - Immunology 2021Quote: ... Nbs and their mixture (1.6 nmol of SARS-CoV-2 RBD mixed with 1.6 nmol of Nbs) were run over a Superdex 75 column (GE healthcare) at a flow rate of 0.5 mL/min with AKTA pure.
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bioRxiv - Immunology 2020Quote: The binding kinetics and affinity of mAbs to SARS-CoV-2 RBD were analyzed by SPR (Biacore T200, GE Healthcare). Specifically ...
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bioRxiv - Immunology 2021Quote: ... was added and the secreted recombinant Spike or RBD protein in culture supernatant were harvested after 72 hours and affinity purified using HisTrap HP Column (GE Healthcare, USA) as described previously [25] ...
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bioRxiv - Cell Biology 2021Quote: ... 32°C or 42°C using a DeltaVision imaging system (Applied Precision Ltd.) composed of an IX-inverted widefield microscope (Olympus ...
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bioRxiv - Cell Biology 2020Quote: ... 32°C or 37°C using a DeltaVision imaging system (Applied Precision Ltd.) composed of an IX-inverted widefield microscope (Olympus ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing RNase E were pooled and loaded on a butyl sepharose HP column (GE Healthcare) which previously was equilibrated in high-salt buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Bioengineering 2020Quote: Binding of SARS-CoV-2 RBD to ACE2 –Fc and ACE2mod–Fc fusion proteins were measured using a Biacore T200 instrument (GE Healthcare). Fusion proteins were first immobilized at a coupling density of ∽1000 response units (RU ...
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bioRxiv - Biophysics 2021Quote: ... The flow through containing SARS-CoV-2 Mpro was collected and further purified using size exclusion chromatography column (G-100, GE Healthcare,) equilibrated with 20 mM Tris ...
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bioRxiv - Immunology 2022Quote: The binding kinetics and affinity of recombinant monoclonal antibodies for the SARS-CoV-2 RBD protein (SinoBiological) were analyzed by SPR (Biacore T200, GE Healthcare). Specifically ...
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bioRxiv - Biophysics 2022Quote: Binding kinetics of ACE2 and SARS-CoV-2 RBDs were determined by surface plasmon resonance using a Biacore S200 (GE Healthcare). All experiments were performed in a running buffer composed of 10 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: The binding kinetics of mAbs to SARS-CoV-2 Delta-RBD or Omicron-RBD monomer were analyzed using SPR (Biacore 8K; GE Healthcare). Specifically ...
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bioRxiv - Microbiology 2022Quote: ... coli BW25113 and the single-gene knockout mutants Δpgm were purchased from Dharmacon (GE Healthcare) and stored in 50% glycerol stock at -80°C ...
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bioRxiv - Plant Biology 2021Quote: ... coli strain BL21 (DE3) and purified with Glutathione Sepharose 4 Fast Flow beads (GE Healthcare) by following the protocol provided by the manufacturer ...
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bioRxiv - Cell Biology 2019Quote: ... GST-fusion proteins were purified from Escherichia coli by glutathione-Sepharose 4B beads (GE Healthcare) and then washed with high salt buffer (20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... coli M55244 cells (ATCC) and purified using Protein L resin (GE Healthcare Cat no. 17547802) with gravity flow affinity chromatography ...
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bioRxiv - Cell Biology 2023Quote: ... coli BL21 and affinity-purified with GST beads (#17-0756-01, GE Healthcare Life Sciences). 293T cells overexpressing indicated proteins were treated with DNA damage reagents and lysed ...
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bioRxiv - Biochemistry 2023Quote: ... coli was subsequently purified by cation-exchange chromatography with a HiTrap™ SP (GE Healthcare) column ...
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bioRxiv - Microbiology 2020Quote: ... The IgG anti-SARS-CoV-2 was purified from plasma of a convalescent patient using a 5/5 HiTrap rProtein-A column (GE Healthcare, USA). See detailed information about this procedure in Supplementary method No.1.
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bioRxiv - Genetics 2021Quote: SARS-CoV-2 receptor binding domain binding to human extracellular ACE2 were analysed on a Biacore T200 instrument (GE Healthcare Life Sciences) at 37°C and a flow rate of 30 μl/min ...
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bioRxiv - Immunology 2020Quote: SEC-purified SARS-CoV-2 RBD-SD1 was immobilized to a Ni-NTA sensor chip in a Biacore X100 (GE Life Sciences) to a response level of ~500 RUs ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli cultures was detected using a UV/visible spectrophotometer (Ultrospec TM 2100 pro, GE Healthcare, UK). The quantification of L-lysine ...
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bioRxiv - Biochemistry 2020Quote: ... B-mode ultrasonography of the chest was performed (18;19) using a Logiq e system (GE Healthcare, Milwaukee, WI) using R5.2.x software and a multifrequency transducer model 12L-RS at a working frequency of 10 MHz ...
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bioRxiv - Developmental Biology 2021Quote: ... nitrocellulose Hybond-C extra (GE Healthcare) membranes were spotted with 0 to 200 μM of PA (Sigma P-9511 ...
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bioRxiv - Biochemistry 2021Quote: ... of L-enantiomeric peptides CDP1 and CDP7 binding to SARS-CoV-2 3CLpro was determined by Surface Plasmon Resonance Spectroscopy (SPR) using a Biacore 8K instrument (GE Healthcare, Uppsala, Sweden). The peptides were immobilised on two separate channels on a series S CM-5 sensor chip (Cytiva ...
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bioRxiv - Molecular Biology 2019Quote: ... coli BL21(DE3) pLysS and purified from inclusion bodies via Sephacryl S-200 gel filtration (GE Healthcare). Stoichiometric amounts of each core histone were incubated together under high salt conditions (2 M NaCl ...
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bioRxiv - Plant Biology 2023Quote: ... coli (BL21 strain) were performed according to the GST Gene Fusion System Handbook (GE healthcare Life Sciences). Recombinant GST-tagged proteins purification were performed according to the manufacturer’s instructions of PierceTM Glutathione Superflow Agarose (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... coli and after cell disruption the cleared lysate was applied to a GSTrap chromatography column (GE Healthcare). After the on-column cleavage with 3C protease (prepared according to in house protocol) ...
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bioRxiv - Plant Biology 2023Quote: ... The protein was produced in Escherichia coli strain Rosetta and purified with nickel Sepharose media (GE Healthcare). EMSA was performed according to the reference guide of the LightShift Chemiluminescent EMSA kit (Thermo Scientific 20148) ...
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bioRxiv - Biophysics 2021Quote: SPR measurements were recorded at either 25°C or 35°C using a Biacore T200 system (GE Healthcare). All experiments were performed using an SA Series S Sensor Chip (GE Healthcare) ...
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bioRxiv - Immunology 2022Quote: ... and stirred overnight (O/N) at 4°C with 4.5 g CM Sephadex C-25 beads (GE Healthcare) preequilibrated for 3 h with 100 mL of buffer A ...
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bioRxiv - Biochemistry 2020Quote: ... coli BL21 cells as described previously.13 The protein was purified using glutathione-Sepharose 4B beads (GE Healthcare) concentrated and flash-frozen in GST buffer [25 mM Tris (pH 7.5) ...
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bioRxiv - Microbiology 2020Quote: ... coli BL21 and were purified by immunoprecipitation with glutathione sepharose 4B beads (Cat: 45-000-139, GE Healthcare). Each reaction with 30 μl final volume contained 50 ng Wheat E1 protein ...
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bioRxiv - Molecular Biology 2022Quote: ... coli similar to the GST-MS2 procedure [20] and then purified on a glutathione-Sepharose column (GE Healthcare). Following elution ...
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bioRxiv - Neuroscience 2020Quote: ... Placement in the spinal cord was confirmed with a commercially-available ultrasound system (LOGIQ e Vet, GE Healthcare, Fairfield, CT) using a linear array 4-10 MHz transducer (8L-RS) ...
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Synaptic Zn2+ potentiates the effects of cocaine on striatal dopamine neurotransmission and behaviorbioRxiv - Neuroscience 2021Quote: ... Slides were dried under stream of cool air and placed on BAS-IP TR 2025 E Tritium Screen (GE Healthcare) for 5-days and imaged using a phosphor imager (Typhoon FLA 7000 ...
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bioRxiv - Bioengineering 2020Quote: Vascular patency was checked using the color doppler mode of an ultrasonic device (LOGIQ e Ultrasound; GE Healthcare, Fairfield, USA). Angiography was also performed with a bolus intravenous injection of 2 ml/kg iohexol (Omnipaque™ ...
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bioRxiv - Microbiology 2020Quote: ... coli BL21(DE3) and were isolated under native purification conditions using a 5 HisTrap High Performance Column (GE Healthcare) with imidazole gradient (10-250 mM ...
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bioRxiv - Biochemistry 2021Quote: ... coli and purified using Ni-NTA affinity resin (Quiagen) and followed with SEC (Superose 6 Increase, GE Life Sciences), as previously described10 ...
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bioRxiv - Neuroscience 2020Quote: ... All GST fusions were expressed in BL21 Escherichia coli and purified with glutathione-Sepharose 4B (GSH beads; GE Healthcare). 100µM of biotinylated- Aβ oligomer and 200µg of GST ...
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bioRxiv - Neuroscience 2022Quote: ... the Aβ42 peptide was recombinantly expressed in BL21*(DE3) pLysS Escherichia coli and purified with DEAE-Sepharose (GE Healthcare). To get rid of large Aβ42 aggregates ...
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bioRxiv - Biochemistry 2020Quote: ... The eluate was diluted two-fold in buffer E + 1 mM DTT + 150 mM NaCl and applied to a MonoS column (GE Healthcare) pre-equilibrated in buffer E + 150 mM NaCl + 1 mM DTT + 0.5 mM EDTA ...
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bioRxiv - Biochemistry 2020Quote: ... Following 3C protease treatment CDK2-cyclin E was buffer exchanged using SEC (Superdex 200 10/300) and then loaded onto a GST-trap column (5ml, GE Healthcare) and then onto an amylose resin (New England Biolabs) ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein quality was assessed by SDS-PAGE and fractions containing RNase E were pooled and loaded onto a butyl sepharose column (GE Healthcare) equilibrated in high-salt buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Physiology 2020Quote: Echocardiography was performed at baseline and following the induction of pulmonary hypertension (PH) (6S probe and Logiq E console, GE Healthcare). Images were acquired in the supine position via a parasternal window.
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bioRxiv - Cell Biology 2019Quote: ... and 0.5 mM PMSF. Mouse anti-Spt6 antibody (25C3; E. Kremmer/A. Schepers) was coupled to Protein A-Sepharose CL-4B (GE Healthcare) beads and stored at 4°C until actual pulldown ...
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bioRxiv - Cell Biology 2021Quote: ... 4 (excepting E) were performed at room temperature using the ring TIRF light path of a DeltaVision OMX SR microscope (GE Healthcare) with a 60x/1.42 numerical aperture (NA ...
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bioRxiv - Neuroscience 2023Quote: ... The course of the radial nerve just above the elbow was visualized using ultrasound (LOGIQ e, GE Healthcare, Chicago, IL, USA). A high-impedance tungsten recording electrode was inserted percutaneously and with ultrasound guidance it was inserted into the nerve ...
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bioRxiv - Microbiology 2024Quote: ... which was imaged by overnight exposition of a BAS-IP MS 2040 E phosphor screen (Cytiva) scanned using a Typhoon FLA 9500 (GE Healthcare).