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Citations for GE Life Sciences :
1 - 50 of 754 citations for SARS Coronavirus Spike Glycoprotein S2 Mosaic C Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 spike protein in dPBS was applied to a 1-ml HiTrap heparin-Sepharose column (GE Healthcare). The column was washed with 5 ml of dPBS and bound protein was eluted with a gradient of NaCl from 150 mM to 1 M in dPBS.
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bioRxiv - Microbiology 2021Quote: Kinetic measurements of the DH1047 Fab binding to SARS-CoV and RsSHC014 spike proteins were obtained using a Biacore S200 instrument (Cytiva, formerly GE Healthcare) in HBS-EP+ 1X running buffer ...
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bioRxiv - Immunology 2022Quote: SPR screening and affinity measurements of monoclonal Fabs binding to SARS-CoV-2 spike proteins were performed using a Biacore S200 instrument (Cytiva, formerly GE Healthcare) in HBS-EP+ 1X running buffer ...
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bioRxiv - Biochemistry 2022Quote: Antibody binding to SARS-CoV-2 spike was assessed using SPR on a Biacore T-200 (Cytiva, MA, formerly GE Healthcare) with HBS buffer supplemented with 3 mM EDTA and 0.05% surfactant P-20 (HBS-EP+ ...
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bioRxiv - Biochemistry 2021Quote: The binding affinities of full IgG antibodies to SARS-CoV-2 spike protein were determined using surface plasmon resonance (SPR) and a BIAcore T200 instrument (GE Healthcare) at 25°C ...
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bioRxiv - Immunology 2021Quote: The binding affinities of antibodies to SARS-CoV-2 spike protein were determined using surface plasmon resonance (SPR) and a BIAcore T200 instrument (GE Healthcare) at 25°C ...
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bioRxiv - Immunology 2022Quote: ... The ACE2 receptor or SARS-CoV-2 spike-specific antibodies (CR3022 or S309) were immobilized on the protein A sensor chip (GE Healthcare) at a ligand capture level of ∼100 RU ...
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bioRxiv - Immunology 2023Quote: The binding kinetics and affinity of monoclonal antibodies to the SARS-CoV-2 spike trimer were analyzed by SPR (Biacore T100, GE Healthcare). Specifically ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant CLASP2 C-term (300 μg, injection volume of 100 μl) was analyzed on a Superose 12 10/300 GL column (GE Healthcare) pre-equilibrated in buffer C ...
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bioRxiv - Microbiology 2020Quote: ... blocked for 1 hour and immuno-detected using Anti-RecA (E. coli) antibody (1000x dilution) (gift from Mike Cox, (52)) and analyzed using an ECL Prime detection kit (GE Healthcare). Densitometry was performed using ImageJ (https://imagej.nih.gov/ij/)
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bioRxiv - Biochemistry 2021Quote: ... which was then used to capture purified HexaPro S2 by the c-terminal twin StrepTag to ~80 response units (RU) in each cycle using a Biacore X100 (GE Healthcare). The binding surface was regenerated between cycles using 0.1% SDS followed by 10 mM Glycine at pH 2 ...
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bioRxiv - Biochemistry 2021Quote: ... isolated E coli membranes with overexpressed MgtA were solubilized in 1% β-dodecyl maltoside (β-DDM) prior to Histrap HP (GE Healthcare) purification equilibrated in (25 mM HEPES pH 7.0 ...
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bioRxiv - Cell Biology 2023Quote: ... Long-term time-lapse imaging was performed using a Deltavision Elite system (GE Healthcare) controlling a Scientific CMOS camera (pco.edge 5.5) ...
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bioRxiv - Microbiology 2020Quote: ... the S glycoprotein was captured by StrepTactin Sepharose High Performance resin (GE Healthcare) and eluted with 10 mM D-desthiobiotin in the binding buffer (20) ...
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bioRxiv - Neuroscience 2020Quote: ... This was expressed in E .coli strain NiCo21 (DE3) using conditions previously described(96) and purified by IMAC using NiHP (GE Healthcare Life Sciences) and AEC using QHP (GE Healthcare Life Sciences ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Recombinant 6xHis-tagged mouse GRP78 was expressed in E coli BL21 cells and purified on a HisTrap™ HP Nickel column (GE healthcare, Uppsala, Sweden). Twenty micrograms (0.3 nmol ...
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bioRxiv - Molecular Biology 2021Quote: ... coli BL21 SI pRARE at 18 °C overnight and purified using a HisTrap FF 5-ml column (GE Healthcare) in standard buffers (50 mM HEPES-KOH pH 7.6 ...
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bioRxiv - Cell Biology 2022Quote: ... S1 and S2 were obtained using a DeltaVision platform (Applied Precision) composed of a customized inverted microscope (IX-71 ...
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bioRxiv - Biochemistry 2020Quote: ... BiP and Grp94 were expressed in Escherichia coli strain BL21* at 37°C and purified via Histrap column (GE Healthcare), by ion exchange on MonoQ column (GE Healthcare) ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 S-2P and SARS-CoV-2 HexaPro S were purified over a Superose6 Increase column (GE Life Sciences).
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bioRxiv - Cell Biology 2023Quote: ... Escherichia coli BL21 (GE Healthcare) was transformed with each GST-fusion protein construct and the expression of GST-fusion protein was induced by 0.1 mM isopropyl-β-D-thiogalactoside (IPTG ...
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bioRxiv - Microbiology 2020Quote: SΔTM and RBD glycoproteins were resolved on SDS-PAGE and transferred onto a nitrocellulose membrane (Amersham Protran, GE Healthcare). Lysates of 293T cells transfected with plasmids encoding full-length spike glycoproteins was also separated by SDS-PAGE for WB ...
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bioRxiv - Cell Biology 2020Quote: ... e Image-Quant LAS 4000 (GE Healthcare). Densitometry analysis of the gels was performed using the Image J software (version 1.37) ...
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bioRxiv - Molecular Biology 2021Quote: ... coli knockout collection (Dharmacon, GE Healthcare). Hedamycin was obtained from the National Cancer Institute’s Developmental Therapeutic Program (NCI DTP ...
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bioRxiv - Plant Biology 2023Quote: ... coli vector pGEX-2TK (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2021Quote: ... Spikes were further purified by Superose 6 increase 10/300 (GE healthcare) size-exclusion column in a buffer containing 2mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: ... The Spike protein was purified on an Akta Express system (GE Healthcare) using a 5mL His trap FF GE Healthcare column in PBS ...
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bioRxiv - Biochemistry 2020Quote: ... for the spike protein and a Superdex 75 10/300 (GE Healthcare) for the RBD and the RBD triple mutant showed a monodispersed and single peak with the expected molecular mass (Supplementary Figure 1).
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bioRxiv - Cell Biology 2022Quote: S2 cells with immunofluorescence staining were imaged with the DeltaVision Core system (Applied Precision) using the Olympus UPlanSApo 100x (n.a ...
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bioRxiv - Neuroscience 2021Quote: ... The slides were dried for 2-3 hours at 30°C and exposed to a Tritium Phosphor Screen (GE Healthcare, Fuji BAS-TR 2025 E) together with calibrated tritium standards (American Radiolabeled Chemicals ...
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bioRxiv - Biochemistry 2023Quote: ... ASK1 TBD was expressed at 25 °C for 18 h and purified from Escherichia coli BL21 (DE3) cells using Chelating Sepharose Fast Flow (GE Healthcare, Chicago, IL, USA) according to the standard protocol ...
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bioRxiv - Microbiology 2020Quote: ... Spike protein was finally purified using Superose 6 HR10/300 column (GE Healthcare) pre-equilibrated with buffer containing 20 mM HEPES (pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... coli and/or immobilized CaM (GE Healthcare), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... coli BL21 cells (GE Healthcare, Freiburg, Germany) expressing GST- or 6xHis fusions were grown to an OD600=0.8 at 37°C in 2YT or LB (6xHis fusions) ...
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bioRxiv - Plant Biology 2022Quote: ... coli using Glutathione Sepharose beads (GE healthcare). The GST-tagged protein-bead complexes were incubated with 2 μg of MBP-tagged proteins in 1 mL of pull-down buffer (50 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... coli BL21 Gold (GE Healthcare, Freiburg, Germany) was diluted into 500 ml of SB medium and incubated at 37°C to an OD600 of 0.8 ...
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bioRxiv - Immunology 2021Quote: ... Purification of E-301 and E-301-LOF was performed directly from transfection harvests using a HiTrap Protein A affinity column (GE Healthcare) and eluted with 1M arginine pH 3.9 ...
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bioRxiv - Immunology 2022Quote: ... Trimeric spike protein was further purified by Superose 6 size-exclusion chromatography (GE Healthcare) in 10 mM Tris pH 8 ...
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bioRxiv - Immunology 2022Quote: ... while the D614G VFLIP spike was purified on a StrepTrap XT column (GE Healthcare), according to the manufacturer’s protocols ...
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bioRxiv - Biochemistry 2019Quote: ... coli and coupled to CNBr-beads (GE Healthcare). The specificity of custom-made antibodies was confirmed by western blotting against human cell lysate after RNAi depletion of target proteins as described previously (Cota et al. ...
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bioRxiv - Biochemistry 2023Quote: ... coli and purified using ÄKTA pure (GE Healthcare) with HiTrap Blue HP ...
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bioRxiv - Immunology 2022Quote: ... The spike proteins were further purified on a Superose 6 gel filtration column (GE Healthcare) in 25 mM Tris pH 7.5 + 150 mM NaCl using an AKTA Pure FPLC instrument (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... coli and purified by Glutathione Sepharose 4B (GE Healthcare), according to the manufacturers’ instructions.
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bioRxiv - Cell Biology 2020Quote: ... coli using the pGEX-5X-3 vector (GE Healthcare). The antiserum was affinity purified using the antigen coupled to Dyna M-280 Streptavidin beads (Life Technologies).
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bioRxiv - Microbiology 2021Quote: ... Escherichia coli BW25113 was purchased from Dharmacon (GE Healthcare). ompC ...
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bioRxiv - Biochemistry 2020Quote: ... coli was performed on an ÄKTApurifier instrument (GE Healthcare) with the modules Box-900 ...
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bioRxiv - Molecular Biology 2021Quote: ... coli and purified with Glutathione SepharoseTM 4B (GE Healthcare). The glycosyltransferase activity assay was carried out with the conditions described by Hou et al ...
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bioRxiv - Microbiology 2021Quote: ... using appropriate oligonucleotides detailed in Supplementary Table S2 and IllustraTM PuReTaqTM Ready-To-GoTM PCR Beads (GE Healthcare) Negative and positive controls were performed with total RNA or genomic DNA as templates ...
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bioRxiv - Neuroscience 2021Quote: ... the E domain was subcloned into pGEX-6P-1 (GE healthcare). The GST-gephyrin E domain fusion protein was purified from E ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 RBD or NL63 RBD was immobilized to a CM5 sensorchip (GE Healthcare) using a Biacore T200 (GE Healthcare ...