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Citations for GE Life Sciences :
101 - 150 of 232 citations for Nickel oxalate dihydrate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... The protein was produced in Escherichia coli strain Rosetta and purified with nickel Sepharose media (GE Healthcare). EMSA was performed according to the reference guide of the LightShift Chemiluminescent EMSA kit (Thermo Scientific 20148) ...
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Undruggable oncoproteins cMyc and NMyc bind to mediator of transcription with superior high affinitybioRxiv - Biochemistry 2024Quote: ... The supernatant was loaded on the His trap HP 5 mL column packed with Nickel (GE Healthcare). FPLC was used for elution of the GST-His6x-TEV-KIX protein with 300 mM imidazole in 50 mM Tris-HCl buffer (150 mM NaCl ...
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bioRxiv - Microbiology 2022Quote: ... the supernatant was loaded on a nickel affinity column (HiTrap™ Chelating HP 5 mL, GE Healthcare) previously equilibrated with 40 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were purified by nickel affinity chromatography using a 5 ml HisTrap HP column (GE Healthcare). The eluted proteins were dialyzed into 20 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... The filtered supernatant included NMnPP protein was applied to a nickel-nitrilotriacetic acid (Ni-NTA, GE Healthcare) affinity column pre-equilibrated with buffer A ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified cell lysate was applied to a nickel affinity column (GE Healthcare; Sepharose high performance beads) equilibrated in Buffer A and purified by FPLC ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was purified using nickel affinity chromatography with a 5 ml HisTrap HP column (GE Healthcare) followed by size exclusion chromatography on a HiLoad Superdex 75 16/600 column (GE Healthcare) ...
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bioRxiv - Microbiology 2024Quote: ... The recombinant proteins were purified using a GE Healthcare HisTrap HP nickel column (GE HealthCare, Chicago, US). Purified proteins were dialyzed at 4 °C for 24 hours using a MW14000 dialysis membrane (Biosharp ...
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bioRxiv - Biochemistry 2024Quote: ... Protein purification was conducted on a nickel affinity column (HisTrap HP, 1mL, GE Healthcare, 17-5247-01) using an ÄKTA Pure 25M Chromatography System (GE Healthcare ...
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bioRxiv - Biophysics 2024Quote: ... The clarified lysate was loaded onto a 5 mL nickel-nitrilotriacetic acid (Ni-NTA) resin (GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2024Quote: ... +4°C) and supernatant was loaded into a 5 mL Nickel sepharose column (HisTrap HP, GE Healthcare). The loaded column was first washed ...
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bioRxiv - Biophysics 2021Quote: ... cell debris was removed and the supernatant loaded onto a 10 ml volume nickel-sepharose column (GE Healthcare) equilibrated with the same buffer ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were purified using an AKTA FPLC with 1 mL nickel-charged HisTrap columns (GE Healthcare Life Sciences) for affinity chromatography of the His-tagged proteins ...
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bioRxiv - Cell Biology 2020Quote: ... The peak fractions were pooled and run on a nickel affinity column (HisTrap FF crude column, GE Healthcare) equilibrated with septin 2 buffer (50 mM Tris-HCl pH 8 ...
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bioRxiv - Molecular Biology 2022Quote: ... Precipitated H-NS was dissolved in nickel binding buffer and applied to a HisTrap Ni2+ column (GE Healthcare). Fractions containing H-NS were mixed with TEV protease to remove the N-terminus His6x-tag ...
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bioRxiv - Bioengineering 2021Quote: ... The protein was purified through nickel-agarose affinity chromatography followed by gel filtration chromatography (S-300, GE Healthcare). The purified protein was concentrated to 30 mg/mL in a buffer containing 20 mM Tris (pH 8.0) ...
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bioRxiv - Biochemistry 2020Quote: Detergent-solubilized TmrAB was next purified by loading onto a Nickel affinity column (HisTrap IMAC HP, GE Healthcare) and washing with binding buffer (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2021Quote: ... Enzymes were isolated from the cleared lysates via nickel affinity chromatography (IMAC) using a HisTrap column (GE Healthcare) and size exclusion chromatography (SEC ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The lysate obtained was loaded onto the pre-equilibrated nickel affinity column (HisTrap FF, 5 mL, GE Healthcare) with loading buffer (50 mM KPi ...
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bioRxiv - Immunology 2021Quote: ... RBD was purified from the supernatants by nickel affinity chromatography with HisTrap Excel columns (GE Healthcare Life Sciences). For protein production used in crystallization trials ...
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bioRxiv - Biochemistry 2023Quote: ... a second nickel-affinity chromatography was performed (His Trap, 5 mL, GE Healthcare; equilibrated in 10% HisB buffer). Sample was loaded and unbound protein further washed out with 10% HisB ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was filtered at 0.8 µm and used for purification by nickel ion affinity chromatography (GE Healthcare). A washing step in the lysis buffer supplemented with 30 mM imidazole was followed by the elution in lysis buffer supplemented with 500 mM imidazole ...
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bioRxiv - Biochemistry 2024Quote: Proteins were purified by immobilized metal-affinity chromatography (IMAC) on a HisTrap HP nickel-Sepharose column (GE Healthcare) at 4°C following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The lysate was centrifuged and the clarified supernatant loaded onto a 5ml HisTrap HP nickel affinity column (GE Healthcare). dCry protein was eluted with 100 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: The filtered lysates were subjected to IMAC using a His-Trap 5 mL Nickel column (GE Healthcare Life Sciences) on an ÄKTA Pure system (GE Healthcare Life Sciences) ...
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bioRxiv - Microbiology 2021Quote: ... and the filtered lysate was passed sequentially through HisTrap Excel nickel and HiTrap CP cation exchange columns (GE Healthcare) to purify the tagged TOP2α protein before incubation overnight with His-tagged TEV protease ...
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bioRxiv - Biophysics 2020Quote: ... 10% glycerol (including 100 mM NaCl and 0.5 mM DTT for PSG)) Nickel Sepharose Fast Flow column (GE Healthcare). Proteins were eluted with 250 mM imidazole and dialysed overnight into 50 mM Tris pH 7.8 (PDZ3 ...
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bioRxiv - Immunology 2020Quote: ... culture media was collected and incubated overnight at 4°C with nickel-Sepharose Excel (GE Healthcare 17-3712-01). His-tagged proteins were eluted from the nickel-Sepharose with 1 M imidazole in TrisCl pH 8.5 ...
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bioRxiv - Plant Biology 2022Quote: ... The filtrate was applied to a 5 mL HisTrap HP Nickel Sepharose™ affinity column (GE Healthcare Life Sciences) and washed with five column volumes of binding buffer (50 mM sodium phosphate buffer ...
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bioRxiv - Immunology 2022Quote: ... the protein-containing supernatant was purified by nickel-affinity and size-exclusion chromatography on an AKTAxpress system (GE Healthcare), which consisted of a 1 mL nickel affinity column followed by a Superdex 200 16/60 gel filtration column ...
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bioRxiv - Microbiology 2024Quote: ... the hexa-histidine tagged H-Rrm4-M protein was purified using Nickel-based affinity chromatography (HisTrap HP, GE Healthcare) on Akta primeplus FPLC system (GE Healthcare) ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting filtrate was loaded onto 2 × 5 ml nickel–nitrilotriacetic acid (Ni–NTA) columns “HisTrapTM HP” (GE Healthcare) equilibrated with lysis buffer 2 ...
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bioRxiv - Immunology 2022Quote: ... the protein-containing supernatant was purified by nickel-affinity and size-exclusion chromatography on an AKTAxpress system (GE Healthcare), which consisted of a 1 mL nickel affinity column followed by a Superdex 200 16/60 gel filtration column ...
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bioRxiv - Molecular Biology 2023Quote: ... The general purification strategy was as follows: (i) Nickel (II) affinity chromatography (Ni Sepharose 6 Fast Flow, GE Healthcare) in 25 mM Tris (pH 8.0) ...
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bioRxiv - Cancer Biology 2023Quote: ... The precipitated proteins were purified based on nickel affinity purification by AKTA Prime Plus automated purification system (GE Healthcare). The sample passed over a 1 mL Nickel-Sepharose (HisTrap HP ...
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bioRxiv - Microbiology 2024Quote: ... Supernatant containing the solubilised membrane proteins was pooled and applied to a His-TRAP nickel affinity column (GE Healthcare) using an ÄKTA Protein Purification System (Cytiva) ...
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bioRxiv - Biochemistry 2024Quote: ... The His-tag containing proteins were loaded onto a HiTrap chelating HP nickel affinity 1-ml column (GE Healthcare). The bound components were eluted with a gradient of 10–300 mM imidazole in 20 mM Tris pH 7.4 and 150 mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... nickel affinity chromatography was performed where the lysate was loaded onto a 5 mL HisTrap™ Excel column (GE Healthcare), followed by a wash with 20 column volumes (CV ...
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bioRxiv - Biochemistry 2021Quote: ... The soluble cell lysate obtained after centrifugation was loaded onto a 5 ml HisTrap FF HP nickel column (GE Healthcare) equilibrated with binding buffer ...
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bioRxiv - Plant Biology 2021Quote: ... The membrane extract was centrifuged for 35 mins at 200,000 x g and the supernatant loaded onto a 10 ml Nickel column (Chelating Sepharose; GE Healthcare) equilibrated in TSB with 0.2% DDM pH 8 ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was consecutively filtered through 0.45 µm and 0.22 µm PES syringe filters prior to loading onto a 5 ml HisTrap nickel affinity column (GE Healthcare) preequilibrated with Buffer A ...
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bioRxiv - Cancer Biology 2022Quote: ... Rac1 protein went through a nickel affinity column followed by a Resource Q column and finally Superdex 75 (GE Healthcare) before concentration to 25mg/ml ...
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bioRxiv - Microbiology 2021Quote: ... The cleared extract was filtered (0.45 μm) before loading onto a nickel-nitrilotriacetic acid affinity column (connected to an AKTAexplorer FPLC system, GE Healthcare) previously equilibrated with lysis buffer ...
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bioRxiv - Neuroscience 2021Quote: ... After centrifugation at 60,000 × g the supernatant was used for purification using nickel-affinity chromatography on a HisTrap HP column (GE Healthcare), using an isocratic elution of low to high imidazole buffer (500 mM imidazole ...
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bioRxiv - Molecular Biology 2020Quote: ... The supernatant was filtered and added to a pre-equilibrated (50 mM Tris, pH 7.8, 10% glycerol) Nickel Sepharose Fast Flow column (GE Healthcare). Proteins were eluted with 250 mM imidazole and dialysed into 50 mM Tris pH 7.8 ...
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bioRxiv - Biophysics 2021Quote: ... the myosin was purified by His-tag affinity purification with a 1 ml HisTrap HP nickel-sepharose column (GE Healthcare). The eluted myosin solution was then dialyzed overnight at 4 °C in a low-salt buffer (25 mM imidazole pH 7.0 ...
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bioRxiv - Microbiology 2021Quote: ... the cultures were spun down at 12,000 g for 30 min and the supernatants were loaded onto a His-Select Nickel Affinity gel (GE Healthcare). Recombinant PstS proteins were eluted with the elution buffer (500 mM imidazole ...
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bioRxiv - Physiology 2021Quote: ... Materials were cleared by a highspeed spin as above and applied to a nickel column (HiTrap Chelating HP, GE Healthcare), washed and eluted in buffer S with imidazole (500 mM).
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bioRxiv - Microbiology 2020Quote: ... the cell supernatant was filtered with a 0.45-μm filter and loaded onto a nickel-charged HisTrap HP column (GE Healthcare). Proteins were eluted with elution buffer (20 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were clarified and the supernatant filtered through 0.22 μm filters and subjected to nickel affinity chromatography using 1 ml HisTrap HP columns (GE Healthcare). Elution was carried out via a 25-500 mM imidazole gradient in 50 mM Tris [pH 8] ...