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Citations for GE Life Sciences :
51 - 100 of 232 citations for Nickel oxalate dihydrate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was loaded on a HisTrap 5 ml nickel-NTA column (GE Healthcare) with a peristaltic pump ...
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bioRxiv - Microbiology 2023Quote: ... His-tagged proteins were isolated using Nickel beads (His Mag Sepharose Ni, GE Healthcare) in the QIAGEN BioSprint machine ...
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bioRxiv - Plant Biology 2023Quote: ... 600 mM NaCl eluates were further subjected to Nickel Sepharose Fast Flow (GE healthcare) and were eluted with 200 mM imidazole ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were applied to Nickel-Nitrilotriacetic (Ni-NTA) His-Trap FF column (GE Healthcare) and 6xHis-tagged-mCherry-p62 was eluted via a stepwise imidazole gradient (50 ...
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bioRxiv - Molecular Biology 2023Quote: ... Supernatant was filtered and loaded onto a Nickel Sepharose Fast Flow column (GE Healthcare). Column was washed with binding buffer and the proteins of interest were eluted with the binding buffer supplemented with 250 mM imidazole and applied to size exclusion chromatography column Hi load 16/60 Sephacryl S-100 column (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... Supernatant was loaded to a 5 ml Nickel-Sepharose HisTrap Chelating column (GE Healthcare) pre-equilibrated with lysis buffer at flow rate of 1.0 ml per minute ...
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bioRxiv - Biophysics 2021Quote: ... This lysate was applied to a nickel affinity column (HisTrap FF 5mL ©Ge healthcare) pre-equilibrated with Ni-A buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cancer Biology 2020Quote: ... purified by nickel-affinity and size-exclusion chromatography on an AKTAxpress system (GE Life Sciences), which consisted of a 1mL nickel affinity column followed by a Superdex 200 16/60 gel filtration column ...
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bioRxiv - Bioengineering 2021Quote: ... and purified by his tag affinity to a nickel column (Histrap, GE healthcare, Chicago, Illinois) via fast protein liquid chromatography (FPLC) ...
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bioRxiv - Bioengineering 2020Quote: All protein constructs were initially purified by IMAC on Nickel Sepharose Excel resin (GE Healthcare). 0.2 µm-filtered supernatants were applied to columns by gravity flow ...
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bioRxiv - Biochemistry 2020Quote: ... the protein supernatant was loaded onto a nickel-nitrilotriacetic acid (Ni-NTA) column (GE Healthcare) containing 0.1 M Ni2+ and equilibrated with the same loading buffer ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was applied to a 5 mL nickel column (HisTrap FF columns, GE Healthcare). The column was washed with 15 mL of W1 buffer (Ni-A + 20 mM imidazole) ...
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bioRxiv - Microbiology 2020Quote: ... The KLKs were isolated via the 6 × His tag using nickel resin (GE Healthcare, Poland) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the supernatant was loaded onto 5 mL Nickel Sepharose 6 fast flow resins (GE Healthcare) in a gravity flow column ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10 mM imidazole and passed over a Nickel column (GE Healthcare Life Sciences). Proteins were purified by affinity chromatography (ÄKTA Pure ...
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bioRxiv - Immunology 2020Quote: ... the His-tagged proteins were purified using nickel-affinity chromatography (HiTrap chelating, HP; GE Healthcare) with an imidazole gradient (10-250 mM ...
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bioRxiv - Biochemistry 2021Quote: ... Filtered lysate was loaded on a pre-equilibrated HisTrap nickel column (GE life sciences 17524701) using ÄKTA Start protein purification system ...
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bioRxiv - Microbiology 2021Quote: The enzyme was purified by nickel affinity chromatography using HisTrap™ HP column (GE Healthcare). Briefly ...
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bioRxiv - Biochemistry 2022Quote: ... onto a pre-equilibrated 5 mL nickel nitrilotriacetic acid affinity column (HisTrap HP, GE Healthcare) with a running buffer (20 mM Tris buffer at pH 7.5 containing 200 mM NaCl and 30 mM imidazole) ...
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bioRxiv - Molecular Biology 2024Quote: ... Nickel affinity chromatography columns (His-Trap HP columns, GE Healthcare, Chalfont St Giles, Bucks., UK) were used to purify the polyhistidine-tagged roGFP2-GPX fusion proteins with a gradient elution (50 mM Tris ...
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bioRxiv - Neuroscience 2023Quote: ... The protein purification was achieved by using the HisTrap nickel column (GE Life Sciences 17524701) for FPLC (ÄKTA start protein purification system ...
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bioRxiv - Biochemistry 2023Quote: ... The recombinant proteins were batch purified on Nickel-Sepharose beads 6 Fast Flow (GE Healthcare) and eluted with 6xHis-TEV protease ...
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bioRxiv - Biochemistry 2023Quote: ... incubated for 40 min at 4°C with nickel phosphate loaded Sepharose beads (GE Healthcare) to separate the His tag ...
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bioRxiv - Synthetic Biology 2023Quote: ... Each protein was purified on a 5ml nickel nitrilotriacetic acid (Ni2+-NTA) resin (GE Healthcare). The column was washed stepwise with 20 ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were purified by nickel affinity chromatography using Ni Sepharose 6 Fast Flow (GE Healthcare) and eluted with 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Molecular Biology 2024Quote: ... filtered with 0.45μm syringe filters and purified using a HisTrap HP nickel column (GE Healthcare) on an Akta FPLC system ...
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bioRxiv - Microbiology 2021Quote: ... The resultant nickel column eluate was loaded onto a 5 ml SOURCE 15Q column (GE Healthcare), instead of 15S column ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant protein was purified by chromatography using a Nickel-loaded Hitrap Chelating HP column (GE Healthcare). Unbound material was extensively washed using Tris 20mM pH8 ...
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bioRxiv - Biochemistry 2021Quote: ... The nickel elution was applied to a HiLoad 16/600 Superdex 200 pg column (GE Healthcare) and run in dPBS (GIBCO 14190-144 ...
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bioRxiv - Microbiology 2021Quote: ... The cleared supernatant was loaded on a 5 ml HisTrap FF nickel affinity column (GE Healthcare), pre-equilibrated with buffer A (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was then applied to Ni2+-NTA (Nickel-Nitrilotriacetic acid) sepharose column (GE Healthcare, USA) pre-equilibrated with the lysis buffer at a flow rate of 0.2 ml/min ...
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bioRxiv - Cancer Biology 2022Quote: ... The lysates was subjected to Nickel gravity column using 5 ml His-Trap columns (GE Healthcare), and protein was eluted with 300 mM imidazole in column volumes ...
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bioRxiv - Immunology 2020Quote: ... Secreted ACE2 PD was isolated from conditioned cell culture medium using Nickel Excel resin (GE Healthcare) followed by gel filtration chromatography on a Superdex200 10/30 column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... and the supernatant was loaded onto a HiTrap chelating HP column charged with nickel (GE Healthcare). Proteins were eluted from the column by a linear gradient (0 to 100% ...
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bioRxiv - Biophysics 2020Quote: ... The soluble fraction of the lysate was loaded on a nickel column (HisTrap Hp, GE healthcare). The column was extensively washed with wash buffer containing 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... supernatant was filtered with a glass fiber filter and loaded over Nickel Excel resin (GE Healthcare) equilibrated with 30 mM MES pH 6.5 ...
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bioRxiv - Biophysics 2020Quote: ... Insoluble particulates were removed by centrifugation and the supernatant applied to nickel-charged agarose (GE Healthcare) preequilibrated in solubilization buffer ...
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bioRxiv - Microbiology 2020Quote: ... The medium was passed over a 5 ml HiTrap Chelating column charged with nickel (GE healthcare), washed with 30 ml of buffer containing 20 mM Tris pH 8 ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was applied to 1 ml Nickel resin (Ni Sepharose 6 Fast Flow, GE Healthcare) preequilibrated with 20 ml buffer A and 20 mM imidazole ...
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bioRxiv - Neuroscience 2023Quote: ... The monomeric proteins were then purified by using the HisTrap nickel column (GE Life Sciences 17524701) for FPLC (ÄKTA start protein purification system ...
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bioRxiv - Cell Biology 2023Quote: ... and the supernatant was injected on HiTrap Chelating HP column loaded with nickel sulfate (GE Healthcare). Proteins were eluted by an imidazole gradient using a fast protein LC Äkta System (GE ...
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bioRxiv - Molecular Biology 2023Quote: ... mv4Int was purified on nickel-nitrilotriacetic acid affinity resin (1ml His-trap HP, GE Healthcare, USA). Column equilibration was performed by injecting 10 column volumes of buffer A ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were purified by nickel affinity chromatography using a HisTrap FF pre-packed column (GE Healthcare). The column was equilibrated with the starting Buffer A ...
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bioRxiv - Microbiology 2021Quote: ... His-tagged Nrf2 protein and MBP-tagged Rev protein were purified with nickel affinity (HisTrap; GE Healthcare), eluted with imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... and the His or His-MBP tag fused proteins by nickel chelated HiTrap Chelating HP (GE Healthcare), followed by a TEV protease cleavage at 16°C overnight ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were purified by immobilized metal-affinity chromatography (IMAC) using pre-packed nickel Sepharose columns (GE Healthcare), or in batch mode on TALON® beads (Takara Bio ...
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bioRxiv - Molecular Biology 2020Quote: ... The soluble supernatants were purified by nickel-affinity chromatography (HiTrap Chelating HP column, GE Healthcare Life Science) using an NGC Quest 10 Plus Chromatography System (BIO-RAD) ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteins were then purified using immobilized metal affinity chromatography (His-Trap-Nickel-chelating columns, GE Healthcare) followed by treatment with TEV protease and a second immobilized metal affinity chromatography step to remove the His-tag ...
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bioRxiv - Microbiology 2021Quote: ... recombinant proteins were purified by nickel affinity chromatography using HisTrap Column (GE Healthcare Life Sciences Pittsburgh, PA). Rat polyclonal antisera against L ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... SE001 was purified from cell supernatants after 5 days of induction using Nickel Excel resin (GE Healthcare), followed by size exclusion chromatography with a Sephadex S200 column (GE Healthcare) ...