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Citations for GE Life Sciences :
101 - 150 of 1700 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Blots were imaged using Amersham ECL prime solution (GE Life Sciences) and a Fusion Pulse 6 imager (Vilber Lourmat) ...
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bioRxiv - Immunology 2020Quote: ... Complexes were detected with the chemiluminescent substrate solution ECL (GE Healthcare).
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bioRxiv - Pathology 2021Quote: ... signals were detected with ECL solution (GE Healthcare, Little Chalfont, UK). Protein levels on the membrane were quantified by densitometry using Gel-Doc software (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting protein solution was purified by Histrap column (GE Healthcare) and then buffer exchanged into DPBS by using desalting column (Thermo) ...
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bioRxiv - Molecular Biology 2022Quote: ... The solutions were loaded onto a Mono Q column (GE Healthcare). Proteins were eluted with a linear gradient to 60% Buffer C (50 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2023Quote: ... Western blots were developed with freshly mixed ECL solutions (GE Healthcare). The antibodies used are the following ...
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bioRxiv - Immunology 2023Quote: ... After centrifugation cells were resuspended in 37.5% Percoll-solution (GE Healthcare) and centrifuged for 5min at 1800rpm ...
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bioRxiv - Genomics 2024Quote: ... Western blots were developed with freshly mixed ECL solutions (GE Healthcare). The following antibodies were used ...
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bioRxiv - Biophysics 2020Quote: ... α-Syn fibrils were formed by buffer exchange of purified monomers into fibril forming buffer (20mM Sodium phosphate, pH7.5) using a PD-10 column (GE Healthcare). The resulting α-Syn solution was passed through a 0.2 µm syringe filter ...
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bioRxiv - Biophysics 2022Quote: ... The excess Cy3-NHS was removed and the buffer was exchanged with a 20 mM MES buffer at pH 5.5 using a PD-10 column (GE Healthcare). The typical labeling efficiency ...
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bioRxiv - Biochemistry 2020Quote: ... 2.5 ml of 20 µM purified Sup35NM were buffer exchanged into Fibril Forming Buffer (FFB - 20 mM sodium phosphate buffer pH 7.4, 50 mM NaCl) using a PD-10 column (GE Healthcare) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... beads were washed once in lysis buffer and rotated at 4ºC overnight in wash buffer containing 8 U/ml thrombin (GE healthcare) to remove the GST tag ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were eluted with 500 mM imidazole followed by buffer exchange to buffer (50mM Tris, 300 mM NaCl, pH 8.0) with HiTrap 26/10 Desalting column (GE Healthcare). To obtain protein without sumo tag ...
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bioRxiv - Biochemistry 2024Quote: ... nanobody was eluted using 2.5 ml of base buffer + 500 mM imidazole directly into base buffer equilibrated PD-10 columns (GE healthcare). 3.5 ml of base buffer was then applied to PD10 columns to elute the nanobody protein base buffer without imidazole ...
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bioRxiv - Biochemistry 2024Quote: ... protein was eluted using 2.5 ml of base buffer + 500 mM imidazole directly into base buffer equilibrated PD-10 columns (GE healthcare). 3.5ml of base buffer was then applied to PD10 columns to elute the RECQL5 protein in base buffer ...
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bioRxiv - Biophysics 2020Quote: ... cysteine variants of α-synuclein were buffer exchanged into PBS or sodium phosphate buffer by use of P10 desalting columns packed with Sephadex G25 matrix (GE Healthcare). The protein was then incubated with an excess of Alexa-488 or Alexa-546 dyes with maleimide moieties (Thermofisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Pooled fractions containing target protein was subjected to buffer exchange with buffer A using a HiPrep 26/10 desalting column (GE Healthcare). Next ...
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bioRxiv - Bioengineering 2021Quote: ... pre-equilibrated with five volumes of imidazole binding buffer (PBS, 10 mM imidazole buffer, pH 7.4) using a fast protein liquid chromatography (FPLC) system (AKTA Pure; GE Healthcare systems). The column was washed with twenty bed volumes of binding buffer (PBS ...
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bioRxiv - Microbiology 2021Quote: ... Bound protein was eluted with elution buffer (lysis buffer containing 250 mM imidazole) and loaded onto glutathione Sepharose resin (GE Healthcare) to separate bound NEC from excess His6-SUMO-UL31 ...
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bioRxiv - Biochemistry 2023Quote: ... 5mM BME) with elution buffer B (buffer A with 500mM Imidazole) on a 5mL His-Trap FF Crude Nickel Column (GE Healthcare). Fractions containing Pol θ were pooled and were separated further on a 5mL HiTrap Heparin HP (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... at 8000 × g then subjected to two rounds of buffer exchange using equimolar Tris-KCl buffer and a 5-mL desalting column (HiTrap Desalting; GE Healthcare) pre-equilibrated with four column volumes of Tris-KCl ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing aerolysin were buffer-exchanged into aerolysin buffer (20 mM Tris, pH 8, 150 mM NaCl) using a HiPrep Desalting column (GE Healthcare). Purified protein was frozen in liquid nitrogen and stored at –20 °C until further usage.
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bioRxiv - Biochemistry 2021Quote: ... Sample was incubated with PreScission Protease (GE Healthcare) overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were transferred to PVDF membranes (GE Healthcare), blocked in 5% non-fat milk (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were transferred to nitrocellulose membranes (GE Healthcare) and incubated in blocking buffer (PBS with 0.05% [w/v] Tween 20 [PBST] with 5% milk for Cas9 and 1% milk for L8C4 ...
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bioRxiv - Immunology 2023Quote: ... Samples then underwent Percoll (GE Healthcare Life Sciences) density gradient centrifugation to remove myelin contamination ...
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bioRxiv - Cancer Biology 2022Quote: ... Live cells were enriched using Ficoll solution (GE Healthcare, 17-5446-02). For density gradient based enrichment of cells ...
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bioRxiv - Biophysics 2022Quote: ... The protein solution was applied to a Phenyl-sepharose HP (GE Healthcare) column equilibrated with 20 mM MES-NaOH pH 6.0 ...
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bioRxiv - Cell Biology 2020Quote: ... The solution was added to glutathione Sepharose 4B (GE Healthcare, Chicago, IL) and incubated 4 h at 4°C under agitation ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein bands were detected using enhanced chemiluminescence (ECL) Select Solution (GE healthcare) and an ECL Pico System (ECL-PS100 ...
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bioRxiv - Neuroscience 2020Quote: ... IPG strips were rehydrated overnight with DeStreak™ Rehydration Solution (GE Healthcare) and 1% (v/v ...
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bioRxiv - Biophysics 2020Quote: ... we load the solution on a 1 mL MonoQ column (GE Healthcare) using an FPLC system (Äkta Explorer) ...
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bioRxiv - Biochemistry 2021Quote: ... and the filtered solution was loaded into a HisTrap column (GE Healthcare). The proteins were eluted with the imidazole gradient ...
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bioRxiv - Plant Biology 2022Quote: ... Resulting solution was applied to SP Sepharose FF (GE Healthcare Life Sciences) equilibrated with the buffer C containing 75 mM (NH4)2SO4 ...
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bioRxiv - Immunology 2022Quote: ... All solutions used were purchased from the equipment supplier (GE Healthcare Biosciences). The procedures described here were performed automatically with the Biacore instrument using pre-established programs for binding amines in peptides or proteins.
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bioRxiv - Biophysics 2023Quote: ... The solution was bound to a HiTrap Butyl HP column (GE Healthcare), washed with 30 cv of high salt buffer (1.5 M NH4SO4 ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions were pooled and buffer-exchanged to sodium carbonate/hydrogen carbonate buffer pH 10 using a Sephadex G-25 column (GE Healthcare, UK). Recombinant protein was stored at −20 °C until required ...
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bioRxiv - Systems Biology 2021Quote: ... GTP-loaded protein was buffer exchanged into either NMR buffer or the GTPase assay buffer using NAP-5 Sephadex G-25 DNA Grade columns (GE Healthcare # 17085301). We were unable to obtain sufficient material for some mutants (H141E/I ...
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bioRxiv - Immunology 2021Quote: ... the protein was reduced with DTT and buffer-exchanged into 100 mM sodium phosphate buffer using a Hi-Trap® desalting column (GE Healthcare). Reduced Hypocrates (0.5 μM ...
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bioRxiv - Bioengineering 2020Quote: ... The running buffer used was HBS-N (GE Healthcare) for the SPR assay ...
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bioRxiv - Biochemistry 2020Quote: ... Buffer was exchanged to PBS with PD10 (GE Healthcare) or CentriPure P96 (emp Biotech ...
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bioRxiv - Biophysics 2022Quote: ... Buffer was exchanged via a PD10 column (GE Healthcare). For tag cleavage ...
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bioRxiv - Microbiology 2020Quote: ... in buffer P using an ÄKTA pure (GE Healthcare) at 0.25 mL min-1 constant flow ...
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bioRxiv - Neuroscience 2020Quote: ... treated with ECL prime blocking buffer (GE healthcare RPN418) for 1 hour and incubated with antibody at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were prehybridized in Rapid-hyb Buffer (GE Healthcare) for 30 minutes at 65°C ...
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bioRxiv - Bioengineering 2024Quote: ... Fc-fusion proteins in HBS-EP+ buffer (GE Healthcare) were immobilized at a capture level of 600-800 response units (RU ...
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bioRxiv - Biochemistry 2023Quote: ... Buffer was exchanged via a PD10 column (GE Healthcare). For tag cleavage ...
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bioRxiv - Microbiology 2023Quote: ... equilibrated in buffer A using AKTA system (GE Healthcare). His-column was washed with buffer A (80 ml) ...
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bioRxiv - Biochemistry 2022Quote: ... pre-equilibrated with buffer B (buffer A supplemented with 1 mM DTT and 2 mM EDTA) connected to an AKTA FPLC system (GE Healthcare Life Sciences). Fractions corresponding to the elution peaks were analyzed by electrophoresis to assess the presence of MreB ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Each Hb solution was then desalted using a PD-10 column (GE Healthcare) equilibrated with 0.01 M HEPES/0.5mM EDTA (pH 7.4 ...