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Citations for GE Life Sciences :
101 - 150 of 3148 citations for 6 Methylimidazo 1 2 a pyridine HCl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... Products of were separated using 6% denaturing sequencing gel for 1 hr at 2000 V and detected at fluorescence filter in the Typhoon FLA (GE Healthcare). Rate of cleavage (nucleotide/minutes ...
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bioRxiv - Microbiology 2021Quote: ... The His-tag itself and traces of uncleaved protein were subsequently removed by nickel affinity chromatography on 1 ml Ni-Sepharose 6 FF resin (GE Healthcare) pre-equilibrated in buffer A ...
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bioRxiv - Biophysics 2021Quote: ... Fifty microliters of the purified RXFP1(1-72) and RXFP2(1-65) at 5 mg/ml was injected over a Superose 6 5/150 increase column (GE Healthcare) equilibrated and eluted with TBS (pH 7.4 ...
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bioRxiv - Plant Biology 2020Quote: ... The resulting supernatant (∼1 ml) was incubated with 20 µl of dimethyl pimelimidate (DMP) cross - linked IgG Sepharose 6 Fast Flow (GE healthcare) resin for 2 h with gentle rotation at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated for 1 h at 4 °C and purified on a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated with 25 mM sodium phosphate pH7.4 ...
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bioRxiv - Molecular Biology 2019Quote: ... and boiled for 5 min at 95°C were separated on 6-8% polyacrylamide/7 M Urea denaturing gel in 1× TBE and transferred onto an Amersham HybondN+ membrane (GE Healthcare). RNA was UV-crosslinked to the membrane ...
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bioRxiv - Biochemistry 2021Quote: ... Mer2 constructs were eluted with a lysis buffer containing 1 mM maltose and passed through a 6 mL ResourceQ column (GE Healthcare) equilibrated in 50 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... were acquired every 1–3 min over a total time period of 4–6 h and deconvolved using the softWoRx software (Applied Precision). In addition ...
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bioRxiv - Genetics 2022Quote: ... Products of were separated using 6% denaturing sequencing gel for 1 hr at 2000 V and detected at fluorescence filter in the Typhoon FLA (GE Healthcare).
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bioRxiv - Plant Biology 2022Quote: ... purified PIN1 and sybody-21 were mixed together with a molar ratio of ∼1:3 and then applied to size-exclusion chromatography using a Superose 6 Increase column (GE Healthcare) in buffer containing 25 mM HEPES pH 7.4 ...
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bioRxiv - Neuroscience 2023Quote: ... Reaction products were electrophoretically resolved on 6% denaturing sequencing gel for 1 hour at 2000 V and detected at fluorescence filter in the Typhoon FLA (GE Healthcare). Nuclease activity quantification compared the densitometric intensity of cleaved versus un-cleaved DNA (ImageQuant ...
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bioRxiv - Biochemistry 2023Quote: ... plate 2 - 1.6 μg/μL carboxylate beads (1:1 mixture of hydrophilic and hydrophobic beads [mass/mass], GE Healthcare Cat ...
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bioRxiv - Cell Biology 2020Quote: ... was exchanged into labelling buffer (20 mM Tris-HCl pH 7.5, 100 mM NaCl) using size exclusion NAP10 columns (GE Healthcare). Protein-containing fractions were identified by protein electrophoresis and pooled ...
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bioRxiv - Microbiology 2019Quote: ... Purified protein was immediately exchanged into TEV cleavage buffer (50 mM Tris-HCl pH 8.0, 10% glycerol) using a 50 ml desalting column (GE Healthcare). For His-tag cleavage ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were eluted from the column with a 10–200 mM imidazole gradient (100 mM Tris-HCl, pH 8.0, 200 mM NaCl) using an ÄKTA Prime Plus chromatography system (GE Healthcare). Fractions were collected and stored at 4°C.
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bioRxiv - Biochemistry 2021Quote: ... The periplasmic extract was adjusted to 50 mM Tris/HCl pH 9.0 (loading buffer) and loaded onto a 5 mL Q Sepharose anion exchange column (GE Healthcare). Proteins were eluted using a linear salt gradient (0-500 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... the purified proteins were dialyzed against TN200 buffer (20 mM Tris-HCl pH 7.5, 200 mM NaCl) using a HiTrap Dessalting column (GE Healthcare), and the protein was further purified by gel filtration chromatography on a HiLoad 16/600 Superdex 75 or HiLoad 16/600 Superdex 200 column (GE Healthcare ...
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bioRxiv - Plant Biology 2020Quote: ... in a high-salt buffer containing 20 mM Tris-HCl (pH 8.0) and 50 mM NaCl in an ÄktaPrime or ÄktaExplorer system (GE Healthcare). The column was calibrated with bovine erythrocyte carbonic anhydrase ...
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bioRxiv - Bioengineering 2022Quote: ... the supernatant was filtered using 0.2 μm filters and buffer exchanged in storage buffer (20 mM Tris-HCl pH7.4, 200 mM NaCl and 5% glycerol) using PD-10 desalting columns (GE healthcare). The desalted sample then was aliquoted and stored at -80°C until the one-step purification/ immobilisation.
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bioRxiv - Biophysics 2022Quote: ... the supernatant was dialyzed against 20 mM Tris-HCl pH 8.0, centrifuged (15 min, 48000xg, 4 °C) and loaded onto a 15 ml Q-sepharose FF (GE Healthcare) column equilibrated with the same buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleaved protein was diluted with 20 mM Tris-HCl pH 7.5 to reach 150 mM NaCl and loaded onto pre-equilibrated HiTrap Q HP column (GE Healthcare). The column was washed with 200 mM NaCl Buffer R (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... and were eluted with CA loading buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 5 mM BME) using AKTAprime plus (GE Healthcare). CA monomer fractions were concentrated using Amicon Ultra-10K device (Merck Millipore ...
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bioRxiv - Microbiology 2021Quote: ... The eluate was then diluted 3x with 20 mM Tris-HCl pH7.5 and passed over a 5 mL Q-sepharose column (GE Healthcare). The Flow through containing the MIF protein was then again loaded on a 5 mL CM sepharose column ...
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bioRxiv - Molecular Biology 2020Quote: ... loaded complexes were further purified in a final size exclusion chromatography step (20 mM Tris-HCl pH 8.5, 150 mM NaCl) using a Superdex 200i column (GE Healthcare), before being used in down-stream applications.
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bioRxiv - Biophysics 2022Quote: ... Obtained PAR was diluted with 20mM Tris-HCl buffer pH 7.5 and was loaded into Resource Q anion exchange 1ml column (GE Healthcare) for fractionation with a linear gradient of 0 - 1M NaCl at 0.25ml/min flow rate ...
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bioRxiv - Biophysics 2022Quote: ... the supernatant was dialyzed against 10 mM Tris-HCl pH 8.0, centrifuged (15 min, 48000xg, 4 °C) and passed over a Q-sepharose FF (GE Healthcare) column ...
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bioRxiv - Biochemistry 2022Quote: ... Further purification was performed in column buffer (25 mM Tris HCl pH 8.0 and 150 mM NaCl) using HiLoad Superdex 200 gel-filtration chromatography (GE Healthcare). The peak fractions were collected ...
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bioRxiv - Genetics 2022Quote: ... Gels were washed for 15 min in 0.25 N HCl before an overnight transfer to a charged nylon membrane (Hybond-XL, GE Healthcare) in 0.4 N NaOH ...
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bioRxiv - Microbiology 2023Quote: ... The eluted fractions containing Cas9-NLS-His were diluted 4-fold with 50 mM Tris-HCl (pH 7.5) and subjected to cation exchange chromatography (Hitrap S HP, GE healthcare) pre-equilibrated with 50 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... and 0.0036% H2O2 in 100 mM Tris-HCl (pH 9.35)) and imaged the chemiluminescence using an ImageQuant LAS-4000 mini Imaging System (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... The purified protein was then dialyzed against 10 mM Tris HCl pH 8.0 and loaded on MonoQ 5/15 column (GE Healthcare) to be eluted using a step gradient of 240 mM and 400 mM NaCl in the same buffer.
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bioRxiv - Molecular Biology 2023Quote: ... Fractions were analyzed for UL12 presence by western blotting using an anti-6xHis antibody and elution fractions from 200 and 300 mM imidazole were combined and the buffer exchange to storage buffer (20 mM Tris-HCL pH7.9, 500mM NaCl, 10% glycerol) using PD-10 desalting columns (GE Healthcare). Protein concentration was calculated using NanoDrop using the UL12 protein extinction coefficient ...
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bioRxiv - Bioengineering 2020Quote: ... Small- (Superose 6 10/300 GL) or large-scale (Superose 6 pre-packed XK 26/70) SEC columns (GE Healthcare, Chicago, IL) were run with 500 µL or 15 mL of supernatant ...
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bioRxiv - Cell Biology 2022Quote: ... 300 μl plasma from WT and KO mice after 6 months of WD feeding were analyzed by FPLC column [Superose™ 6 Increase 10/300 GL FPLC column (GE Healthcare)] ...
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bioRxiv - Biochemistry 2020Quote: ... and applied to Superose 6 16/50 (GE Healthcare) column equilibrated with gel filtration buffer (20 mM HEPES pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... Superose 6 increase 3.2/300 GL (29091598, GE Healthcare) and Superdex 200 increase 3.2/300 GL (28990946 ...
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bioRxiv - Biochemistry 2020Quote: ... and further purified by Superose 6 increase (GE Healthcare) running in HBS (20 mM Hepes pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... and further purified by Superose 6 increase (GE Healthcare) running in a buffer containing 20 mM Tris (pH 7.5) ...
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bioRxiv - Biophysics 2020Quote: ... p97 was purified by Superose 6 column (GE Healthcare).
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bioRxiv - Biophysics 2020Quote: ... and further purified by Superose 6 increase (GE Healthcare) running in a buffer containing 20 mM Tris (pH 7.5) ...
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bioRxiv - Biochemistry 2022Quote: ... loaded onto a Superose 6 Increase column (GE Healthcare) pre-equilibrated with FSEC buffer (20 mM HEPES ...
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bioRxiv - Molecular Biology 2022Quote: ... Superose 6 Increase 10/300 GL column (GE Healthcare) was equilibrated with the twice volume of 1× lysis buffer without PMSF ...
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bioRxiv - Biochemistry 2020Quote: ... or Superose 6 10/300GL column (GE Life Sciences). There are 24 100ul fractions collected by using MAbPac SEC-1 ...
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bioRxiv - Molecular Biology 2019Quote: ... The Superose 6 increase 10/300 column (GE Healthcare) was pre-equilibrated with running buffer (50 mM HEPES pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... Ni2+-Sepharose™ 6 fast flow resin (GE Healthcare) was equilibrated with 10 column volumes of 40 mM KPi ...
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bioRxiv - Biochemistry 2022Quote: ... The Superose 6 increase 10/300 column (GE Healthcare) was pre-equilibrated with glycerol-free Ndc80 buffer (50 mM HEPES pH 8.0 ...
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bioRxiv - Molecular Biology 2019Quote: ... and α-mouse HRP-linked F(ab’)2 (GE Life Sciences NA9310; 1:5000). Blots were visualized by chemiluminescence with the SuperSignal West Femto kit (Pierce ...
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were purified over 1-2 ml His-Trap FF columns (GE Healthcare) with elution buffer containing 250 mM Imidazole ...
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bioRxiv - Cell Biology 2021Quote: ... For co-immunoprecipitation of endogenous SQSTM1/p62 the cell lysates were incubated for 2-4 h with specific antibody followed by 1-2 h with protein-G coupled Sepharose beads (GE Healthcare, 17-0885-01). To resolve protein complexes for denaturing immunoprecipitation ...
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bioRxiv - Microbiology 2021Quote: ... The reaction was stopped by addition of 6 mM β-mercaptoethanol and mixtures were applied to a 1 ml HisTrap HP Ni2+ column (GE Healthcare) before elution using a gradient of Buffer B (50 mM Tris pH 7.5 ...