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Citations for GE Life Sciences :
151 - 200 of 3237 citations for 6 Methylimidazo 1 2 a pyridine HCl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were purified over 1-2 ml His-Trap FF columns (GE Healthcare) with elution buffer containing 250 mM Imidazole ...
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bioRxiv - Cell Biology 2021Quote: ... For co-immunoprecipitation of endogenous SQSTM1/p62 the cell lysates were incubated for 2-4 h with specific antibody followed by 1-2 h with protein-G coupled Sepharose beads (GE Healthcare, 17-0885-01). To resolve protein complexes for denaturing immunoprecipitation ...
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bioRxiv - Microbiology 2021Quote: ... The reaction was stopped by addition of 6 mM β-mercaptoethanol and mixtures were applied to a 1 ml HisTrap HP Ni2+ column (GE Healthcare) before elution using a gradient of Buffer B (50 mM Tris pH 7.5 ...
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bioRxiv - Genetics 2024Quote: ... Cell debris was removed by centrifugation and the supernatant was incubated with 1 mL of equilibrated Ni-Sepharose 6 Fast Flow resin (GE Life Sciences) and gently mixed in batch for 1 hr at 4° C ...
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bioRxiv - Cell Biology 2020Quote: ... All capture surfaces were regenerated with one 30-s pulse of 10 mM glycine–HCl (pH 1.5, (GE Healthcare, Marlborough, MA). Kinetic parameters were obtained by globally fitting the data to a 1:1 binding model with mass transport limitation using Scrubber 2.0c Evaluation Software ...
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bioRxiv - Biophysics 2024Quote: ... pure fractions were buffer-exchanged into the final buffer (20 mM Tris-HCl, pH 8, and 150 mM NaCl) using a HiPrep Desalting column (GE Healthcare) and stored at -20°C in the final buffer ...
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bioRxiv - Microbiology 2023Quote: ... equilibrated with buffer A (50 mM Tris-HCl at the appropriate pH, 300 mM NaCl) on an AKTÄ explorer 10S FPLC system (GE Healthcare). The column was washed with 10 column volumes of buffer A ...
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bioRxiv - Biophysics 2023Quote: ... The fractions containing fusion protein were pooled and exchanged into a SUMO protease cleavage buffer (20 mM Tris-HCl at pH 8.0, 0.15 M NaCl) using a HiPrep 26/10 column (GE Healthcare Life Sciences). The cleavage reaction was initiated by adding 6×His-tagged SUMO protease to a final concentration of ∼8 μg/mL to the protein solution ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... with 10 mM PTI and PTI alone in water (2 mL) were loaded into 2 mL mini-dialysis tubes with 1 kDa molecular weight cut-off (GE Healthcare Bio-sciences Corp. USA). The tube was immersed in a 200 mL closed Duran flask which was placed in a water bath at 37°C for the duration of the experiment ...
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bioRxiv - Molecular Biology 2020Quote: ... 20 µl IgG Sepharose 6 Fast Flow beads (GE Healthcare) were washed twice with 1 ml ice-cold PA-150 buffer [150 mM KCl ...
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bioRxiv - Bioengineering 2019Quote: ... 6% Ficoll PM-400 (17-0300-10, GE Healthcare, Sweden), 0.2% sarcosyl (L7414 ...
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bioRxiv - Microbiology 2019Quote: ... Superose 6 10/300 GL (GE Healthcare, Chicago, IL, USA) equilibrated with 10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2019Quote: ... 6 mL cesium trifluoroacetate solution (CsTFA, GE Healthcare, Buckinghamshire, UK) and gradient buffer solution ...
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bioRxiv - Biochemistry 2021Quote: ... and finished with SEC (Superose 6 Increase, GE Life Sciences). Recombinant PA28γ was expressed in Rosetta E ...
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bioRxiv - Biochemistry 2021Quote: ... size-exchange chromatography (SEC) (Superose 6 Increase, GE Life Sciences) was performed on pooled fractions with proteolytic activity after Resource Q ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were loaded in a SuperoseTM 6 Increase (GE Healthcare) column ...
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bioRxiv - Microbiology 2020Quote: ... Affinity chromatography using Ni Sepharose 6 Fast Flow (GE Healthcare Life Sciences ...
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bioRxiv - Biophysics 2022Quote: ... SUR1core was further purified using Superose 6 increase (GE Healthcare) column running with TBS supplemented with 0.006% GDN ...
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bioRxiv - Cell Biology 2022Quote: ... before subjecting it to SEC (Superose 6 column, GE healthcare). For labeling purified KASH5 used in TIRF experiments ...
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bioRxiv - Immunology 2020Quote: ... Size exclusion chromatography using Superose 6 Increase column (GE Healthcare) yielded final purified protein.
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bioRxiv - Molecular Biology 2020Quote: ... followed by Superose 6 10/300 GL column (GE Healthcare) to a final concentration of 180 μg/ml in PBS.
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bioRxiv - Immunology 2022Quote: ... and a Superose 6 10/300 GL column (GE Healthcare) in 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2022Quote: Purified ORF8N78Q was loaded onto a Superose 6 (GE Healthcare) gel filtration column and eluted in 20 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: A nickel column (Ni Sepharose 6 Fast Flow, GE Healthcare) was washed with deionized water at pH 7.0 ...
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bioRxiv - Cell Biology 2019Quote: ... Lysates were incubated with IgG sepharose 6 beads (GE Healthcare) in XB buffer (100 mM KCl ...
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bioRxiv - Biophysics 2021Quote: ... using a Superose 6 increase 10/300 column (GE Healthcare) and an ÄKTA system in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... BLM was further purified using a Superose 6 (GE Healthcare) in buffer D (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... and injected to Superose 6 10/300 column (GE Healthcare) previously equilibrated in size exclusion chromatography (SEC ...
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bioRxiv - Genetics 2022Quote: ... or IgG Sepharose® 6 Fast Flow Beads (GE Healthcare) was added ...
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bioRxiv - Biochemistry 2024Quote: ... using a Superose 6 increase 10/300 column (GE Healthcare) and an Agilent LC-1220 system in 20 mM Mes-Tris pH 6.5 ...
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bioRxiv - Biochemistry 2024Quote: ... using a Superose 6 increase 10/300 column (GE Healthcare) and an Agilent LC-1220 system in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... IgG Sepharose 6 Fast Flow beads (20 µl, GE Healthcare) were equilibrated by one wash step using 1 ml of ice-cold TST buffer (50 mM Tris pH 7 ...
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bioRxiv - Cell Biology 2020Quote: ... the corresponding cDNAs of mouse angulin-1 were amplified by PCR using KOD-Plus-Ver.2 DNA polymerase and subcloned into pGEX-6P-1 (GE Healthcare). To construct an expression vector for MBP-tagged N-ZO-1 (aa 1-862) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg lysates were incubated with the appropriate agarose-conjugated primary antibody for 3-4 h at 4°C or with unconjugated antibody (1-2 mg) overnight at 4°C followed by 1 h incubation with Protein G Sepharose beads (GE Healthcare). Immuno-complexes were washed four times with NETN buffer (20 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... containing 1 mM 2-ME) and Superdex 200 Increase 10/300 GL column (GE Healthcare; 10 mM KPB [pH 7.0] containing 50 mM NaCl and 1 mM 2-ME) ...
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bioRxiv - Plant Biology 2022Quote: ... The primary antibody was diluted 1:10,000 in TBST-Buffer with 2% Blocking (GE Healthcare) and incubated on the membrane for 2 h ...
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bioRxiv - Biochemistry 2022Quote: ... lysates in PBS with protease and phosphatase inhibitor cocktails were injected (500 µL per injection, corresponding to 1–3 mg total protein) onto a Superose® 6 10/300GL column (GE Life Sciences) equilibriated with PBS (pH 7.2 ...
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bioRxiv - Immunology 2020Quote: Purified CoV-S trimers were concentrated to 1 mg/mL and loaded onto a Superose 6 Increase 10/300 GL column (GE Healthcare Life Sciences) and passed through a Wyatt DAWN coupled to a Wyatt UT-rEX differential refractive index detector (Wyatt Technology) ...
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bioRxiv - Biochemistry 2020Quote: ... equilibrated in protein buffer (10 mM Tris-HCl pH 8.0, 150 mM NaCl) and connected to an Äkta Purifier FPLC system (GE Healthcare, Boston, U.S.A.). The column was washed with five column volumes and subsequently eluted with three column volumes of protein buffer containing 5 mM d-desthiobiotin ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were eluted from the column with a 10–200 mM imidazole gradient (100 mM Tris-HCl, pH 8.0, 200 mM NaCl) using an ÄKTA Prime Plus chromatography system (GE Healthcare, Chicago, IL). Fractions were collected and stored at 4°C and the presence of GSTU7-His protein was confirmed by analyzing the collected fractions by SDS-PAGE.
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bioRxiv - Bioengineering 2023Quote: ... 35 mM NaCl and 20 mM Tris HCl pH 8.3) and then purified by ion exchange using a Hi Prep Q FF 16/10 column (GE Healthcare Life Sciences) connected to an Akta Pure system (Cytiva) ...
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bioRxiv - Cancer Biology 2021Quote: ... incubated at 4°C overnight with primary antibody (1:1000) and incubated for 1 hour at RT with secondary antibodies in either 2% PBA (GE healthcare, RPN418), 5% ELK or OBB (LI-COR ...
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bioRxiv - Biochemistry 2021Quote: Three microliters of S-D614G in 1.2 mg/ml was mixed with 1.2 equivalent of RBD-chAb45 and incubated at room temperature for 1 hr before being separated by SEC using a size-exclusion column (Superose 6 increase 10/300 GL; GE Healthcare, U. S. A.) in TN buffer ...
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bioRxiv - Biochemistry 2021Quote: ... and incubated at room temperature for 1 hr before being separated by SEC using a size-exclusion column (Superose 6 increase 10/300 GL; GE Healthcare, U. S. A.) in TN buffer ...
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bioRxiv - Cell Biology 2020Quote: ... 2) the reaction mix was purified using anion exchange column (HiTrap QFF 1 mL; GE Healthcare); 3 ...
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bioRxiv - Cancer Biology 2021Quote: ... and after 1 and 2 days of co-culture on an INCell Analyzer 2200 (GE Healthcare), with a large field-of-view sCMOS camera (resolution 2048×2048 pixels ...
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bioRxiv - Microbiology 2021Quote: ... 2 mM β-mercaptoethanol) and applied to a 1-ml Hi-Trap Heparin column (GE Healthcare) equilibrated with TGED ...
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bioRxiv - Neuroscience 2020Quote: The two animals (Monkey 1 and Monkey 2) were scanned on a Discovery MI (GE Healthcare) PET/CT scanner ...
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bioRxiv - Biochemistry 2020Quote: ... applied onto a Superdex 6 column (10/300 Increase, GE Healthcare) and eluted in a buffer containing 25 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2019Quote: ... Size exclusion chromatography (Superose-6 increase 10/300 column, GE Healthcare) was then performed in Gel Filtration Buffer with a Shimadzu High Performance Liquid Chromatograph ...