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Citations for GE Life Sciences :
101 - 150 of 550 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Gephyrin and LS variants were further purified via size exclusion chromatography (Superdex 16/600 prep grade, GE Healthcare, Chicago, Illinois) in ITC-buffer ...
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bioRxiv - Plant Biology 2022Quote: ... and 250 mM imidazole and further purified by size exclusion chromatography (Hiload 16/60 Superdex 200 prep grade, GE Healthcare) in a buffer containing 10 mM Bis-Tris pH 6.0 ...
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bioRxiv - Microbiology 2023Quote: ... His6-tagged protein was further purified using a HiLoad 16/60 Superdex 200 prep-grade size exclusion column (GE Healthcare) in 50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... Eluate fractions were pooled and loaded onto a size exclusion column (HiLoad Superdex 26/600 200 prep grade, GE Healthcare) pre-equilibrated with Tris buffer (50 mM Tris ...
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bioRxiv - Microbiology 2023Quote: ... the viral lysate was filtered through a 1.2 µm pore size glass microfiber filter (grade GF/C, GE Healthcare Whatman) followed by a 0.45 µm pore size Nalgene Rapid-Flow filter unit (PES ...
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bioRxiv - Biochemistry 2024Quote: ... and injected onto either a Superdex 75 or Superdex 200 Hiload 16/60 prep grade gel-filtration column (GE Healthcare), pre-equilibrated with gel filtration buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... using 10mM sodium acetate pH4.0 solution (GE Healthcare). The surface of the sensor chip was blocked with 1M ethanolamine-HCl pH8.5 (GE Healthcare) ...
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bioRxiv - Biophysics 2024Quote: ... 1% Antibiotic Antimycotic Solution (GE Healthcare Life Sciences), and 0.5 mg/mL of the aminoglycoside antibiotic G418 (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using GFXTM PCR DNA and Gel Band Purification Kit (GE Healthcare). Restriction endonucleases and T4 DNA ligase were purchased from Thermo Fisher Scientific and PrimeSTAR DNA polymerase from Takara Bio ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant containing the eluted and cleaved protein was concentrated down and applied onto a Superdex 75 column (16/60 prep grade, GE Healthcare) and eluted with a buffer containing 25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... the affinity tag was removed from complexes collected after the nickel elution by an overnight TEV digestion against lysis buffer followed by size-exclusion chromatography using a HiLoad 16/60 Superdex 75 prep grade column (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... The eluted fractions were concentrated to 5 ml and loaded onto a HiLoad 26/600 Superdex 200 prep grade (GE Healthcare) equilibrated with the SEC buffer (50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Biochemistry 2021Quote: ... MBP-PIPI was subjected to HRV-3C cleavage at 4°C overnight and was further purified in HiLoad 16/60 Superdex 200 preparatory grade size exclusion column (GE Healthcare) to separate fractions of polymerase proteins from cleaved MBP and protease ...
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bioRxiv - Biophysics 2021Quote: ... Pooled protein fractions were deglycosylated with endoglycosidase F1 overnight at 4C and then concentrated for further purification with a size exclusion column (HiLoad 16/60 or 26/60 superdex 75 prep grade columns, GE Healthcare) in a buffer containing 20 mM Hepes (pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... EDTA was added to 1 mM and the PykA was then loaded onto a HiLoad 26/60 Superdex 200 Prep Grade gel filtration column (GE Healthcare) equilibrated in buffer C (500 mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... The His6-tag was removed by thrombin cleavage and the proteins further purified with a HiLoad 16/60 Superdex 75 prep grade column (GE Healthcare) in 20 mM Tris HCl (pH 7.4) ...
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bioRxiv - Microbiology 2021Quote: ... HisTRAP eluted peak fractions were pooled and applied to a Hi-Load 16/600 Superdex-200 (preparation grade) size-exclusion column (GE Healthcare) equilibrated with the gel filtration buffer (50 mM Tris-HCl ...
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bioRxiv - Immunology 2021Quote: ... The peak fractions were collected and purified by size exclusion chromatography using Hi-Load 16/60 Superdex-200 (prep-grade) gel filtration column (GE Healthcare) in a buffer containing 25 mM Tris·HCl (pH 8.0 ...
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bioRxiv - Immunology 2021Quote: ... Peak protein fractions were pooled and loaded onto the size-exclusion chromatography column using Hi-Load 16/60 Superdex-200 (prep-grade) gel filtration column (GE Healthcare) in a buffer containing 25 mM Tris·HCl (pH 8.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... size-exclusion chromatography of all proteins was carried out on either HiLoad 26/60 or 16/600 Superdex 75 prep grade column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2022Quote: ... After dialysis the supernatant containing folded tetramers and dimers were subjected to Superose 6 prep grade XK 16/70 size exclusion column (GE Healthcare) purification using HFB buffer ...
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bioRxiv - Biochemistry 2021Quote: ... His-SUMO-Tau peptide was purified by affinity chromatography on Ni-NTA resin followed by size exclusion chromatography (Hiload 16/60, Superdex 75, prep grade, Cytiva) in SPR buffer (HBS-EP+, GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... 3 µl of protein sample diluted to a concentration of between 0.02 mg/ml and 0.05 mg/ml was added to a grid and then manually blotted away with Whatman Grade 1 filter Paper (GE Healthcare). 3 µl of 0.75% Uranyl Formate was then added to the grid and blotted away ...
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bioRxiv - Biochemistry 2023Quote: ... Then the protein was subjected to size-exclusion chromatography using HiLoad Superdex 200 prep-grade 16/600 column (GE Healthcare Biosciences) pre-equilibrated with buffer F (50 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... stored in sample loops on the system and injected into a Gel Filtration column (HiLoad 16/60 Superdex 200 prep grade, GE Healthcare). The protein sample was concentrated using Vivaspin 20 filter concentrators (VivaScience ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was concentrated and further purified by size-exclusion chromatography using a HiLoad 16/600 Superdex 200 prep grade column (GE Healthcare) in 20 mM Tris ...
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bioRxiv - Biophysics 2023Quote: VD1 hydrodynamic properties were approximated by SEC using a HiLoad 16/60 Superdex 75 prep grade (S-75) column (GE Healthcare) in 20 mM Tris ...
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bioRxiv - Microbiology 2023Quote: ... All SpyCEP constructs were subsequently concentrated and purified by size exclusion chromatography on a HiLoad Superdex 200 or 75 prep grade column (GE Healthcare) dependent on molecular weight.
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bioRxiv - Biochemistry 2023Quote: ... The untagged sF or sG was further purified with size-exclusion column (HiLoad 16/60 Superdex 200 prep grade XK 16 gel filtration column (GE Healthcare). The buffer PBS was used for sG and the PBS with 0.01% Triton X-100 was used for sF purification.
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bioRxiv - Molecular Biology 2024Quote: ... All proteins underwent an additional purification step by size exclusion chromatography (SEC) on a HiLoad 16/60 Superdex 200 prep grade column (GE Healthcare) in SEC Buffer (50 mM Na2HPO4 ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was further purified using ion exchange chromatography preceding size exclusion chromatography on a HiLoad 16/600 Superdex 75 prep grade column (GE Healthcare) into CRY buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... A final size exclusion chromatography (SEC) step was performed using a HiLoad 16/600 Superdex 200 prep grade column (GE Healthcare), with running buffer 20mM HEPES 7.4 ...
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Faa1 membrane binding drives positive feedback in autophagosome biogenesis via fatty acid activationbioRxiv - Biochemistry 2023Quote: ... The cleaved protein was concentrated and loaded onto a Superose 6 Increase size exclusion chromatography column (10/300 prep grade, GE Healthcare). Elution was carried out with 150 mM NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... As done in our previous experiments (Cattaneo et al., 2022) stimuli aliquots were spread on grade 1 – 20 mm circles filter paper (GE Healthcare Life Science ...
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bioRxiv - Neuroscience 2023Quote: ... The cleaved protein was then separated from tag and TEV by a second Ni-NTA affinity column and further purified using size exclusion chromatography on a HiLoad 16/600 Superdex 75 prep grade column (GE Healthcare) in 50 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: ... Eluates containing myrArf1•GDP were further purified by size exclusion chromatography (SEC) using a HiLoad 26/600 Superdex 75 prep grade column (GE Healthcare) pre-equilibrated and then run in an isocratic manner using fresh TNMD buffer at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Final polishing of the target proteins was done through size exclusion on a HiLoad 26/600 Superdex 75 prep grade column (GE Healthcare) eluting in PBS with 1 mM DTT ...
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bioRxiv - Biophysics 2023Quote: ... The cleaved protein of interest was then purified by size exclusion chromatography using a Superdex 75 prep grade column (GE Healthcare). Where necessary ...
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bioRxiv - Biophysics 2022Quote: ... cleaved proteins were separated by a reverse Ni-NTA affinity chromatography step and subsequently purified via a HiLoad prep grade 16/60 Superdex200 or 16/60 Superdex75 column (GE Healthcare) equilibrated with 20 mM Tris pH 7 ...
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bioRxiv - Biochemistry 2024Quote: ... Lysate was loaded on a HiLoad™ 16/600 Superdex 200 preparation grade gel filtration column and installed in a liquid chromatography system (Aekta Purifier) from GE Healthcare. A protein size standard was used as a reference ...
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bioRxiv - Biochemistry 2024Quote: Gel filtration was performed using concentrated IMAC purified proteins of 500µl in volume using a Superdex 75 GL column and Superdex-200 10/300 GL column preparative grade size exclusion chromatography column (GE Healthcare). The column was pre-equilibrated with SEC buffer composed of 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... The concentrated proteins were finally purified by gel filtration chromatography using Hiload 16/60 Superdex 200 prep grade column (GE healthcare) in buffer C and concentrated in the same way as the binary complex ...
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bioRxiv - Biochemistry 2024Quote: ... The peak fractions were further purified by size exclusion column chromatography using a Hiload 16/60 Superdex-200 prep grade column (GE healthcare) in buffer C (50 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... Biotin-labelled PCR amplicons were captured by mixing PCR products with streptavidin-sepharose beads (GE Healthcare). Vacuum Prep Tool with filter probes was used to capture sepharose beads with biotin labelled PCR amplicons ...
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bioRxiv - Microbiology 2021Quote: ... The DNA of purified PCR products (GFX PCR DNA and Gel Band Purification Kit GE Healthcare) was sequenced using primer Map Tn5 B fwd.
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bioRxiv - Cell Biology 2020Quote: ... membranes were incubated with enhanced chemiluminescence solution (GE Healthcare) and exposed to X-ray films (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... and exposed to enhanced chemiluminescence solution (RPN2235, GE Healthcare). Immunoreactive protein bands were imaged using an iBright FL1500 instrument (ThermoFisher ...
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bioRxiv - Evolutionary Biology 2022Quote: ... before being rehydrated in Destreak rehydration solution (GE healthcare) containing 1% IPG (immobilised pH gradient ...
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bioRxiv - Biophysics 2021Quote: ... Excess free dye was removed by size-exclusion chromatography using the Superdex® 200 Increase 10/300 GL (prep-grade) gel filtration column (GE Healthcare) and gel filtration buffer at a flow rate of ~0.4 mL/min for separation of free dye from labeled protein ...
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bioRxiv - Cell Biology 2022Quote: ... The peak fractions were pooled and loaded onto a Superdex 200 HiLoad 16/60 preparative-grade column (GE Healthcare, 17-1069-01) in 5 mL injections and developed at room temperature with the size exclusion buffer (20 mM Tris ...