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Citations for GE Life Sciences :
1401 - 1450 of 2265 citations for 8 9 5 α CHOLESTEN 24 METHYLENE 3 β OL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... followed in tandem by a HiTrap™ Q Sepharose HP column (5 ml/min, GE Life Sciences). The SP Sepharose column was removed before the protein was stepped off from the Q sepharose with Buffer B2 (20 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting protein fraction was further purified on a 5 ml Hi-Trap HP SP (GE Healthcare) cation exchange column using an Akta Pure FPLC ...
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bioRxiv - Molecular Biology 2020Quote: ... Rubisco-containing fractions were applied to an anion exchange column (MONO Q 5/50 GL, GE Healthcare) and eluted with a linear salt gradient from 30 to 500 mM NaCl in lysis buffer.
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bioRxiv - Microbiology 2020Quote: ... Purification was done using 5 mL pre-packed Ni-sepharose AKTA Hi-TRAP column (GE Healthcare, USA) equilibrated (100 mM NaH2PO4 ...
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bioRxiv - Cell Biology 2021Quote: ... The next morning the cleaved protein was loaded on a 5 ml HiTrap HeparinHP column (GE Healthcare). The protein was eluted over a gradient from 100 mM to 2M KCl ...
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bioRxiv - Biophysics 2021Quote: ... the solution was loaded onto a 5 mL Ni-NTA HiTrap chelating column (GE Healthcare, Chicago IL) that was equilibrated in Ni-NTA base buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... 100 mM NaCl pH 7.0 and further purified on a 5 ml Heparin affinity column (GE Healthcare) with a 100 ml gradient of 0.1 to 1.5 M NaCl ...
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bioRxiv - Genomics 2020Quote: ... The oligonucleotide pool was purified using 2 x illustra NAP-5 Columns (GE Life Sciences, PN: 17085302). The resulting solution was evaporated to dryness ...
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bioRxiv - Biochemistry 2022Quote: ... and 5 was further purified by gel filtration on a 16/600 Superdex 200 column (GE Healthcare) in S200 buffer ...
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bioRxiv - Biochemistry 2022Quote: ... or using an ÄKTA Prime purification system (Amersham Biosciences) equipped with 5 mL HisTrap column (GE Healthcare), with an imidazole gradient of buffer B (20 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was then loaded onto a 5 ml HiTrap SP FF column (GE Healthcare Life Sciences) followed by gradient elution with elution buffer SB (50 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2022Quote: ... and loaded at 1 mL/min onto a Ni-NTA column (HisTrap HP, 5 mL; GE Healthcare) that had been previously equilibrated with Ni-NTA Buffer (NB ...
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bioRxiv - Immunology 2022Quote: ... the sample was again loaded onto a HisTrap™ HP 5 ml column (GE Healthcare, Diegem, Belgium). The flow-through was collected and concentrated before injection onto a HiLoad® 16/600 Superdex® 75 pg column (GE Healthcare Diegem ...
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bioRxiv - Physiology 2022Quote: ... Leukocytes were separated from debris by resuspending the pellet in 5 ml 33% Percoll solution (GE Healthcare). Erythrocytes were lyzed using an ACK lysis buffer (155 mM NH4Cl ...
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bioRxiv - Cell Biology 2022Quote: ... then loaded onto a 5 mL Histrap column (Cytiva) connected to an FPLC AKTAxpress system (GE Healthcare). After affinity binding ...
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bioRxiv - Biochemistry 2022Quote: ... and 0.5 mM EDTA) and loaded onto a 5 mL RESOURCE Q anion exchange chromatography (GE Healthcare). Hsp104 was eluted via linear gradient of buffer Q+ (20 mM Tris pH = 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... 5 mL of supernatant was collected and loaded to a Sephacryl S-300 HR column (GE healthcare) pre-balanced with G-buffer ...
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bioRxiv - Immunology 2022Quote: ... Filtrated culture supernatants were purified with a 5 ml HisTrap FF Crude column (GE Healthcare Life Sciences) previously equilibrated in Buffer A (20 mM NaH2PO4 ...
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bioRxiv - Neuroscience 2021Quote: ... Sample was concentrated to ∼5 mg/mL and injected onto a Superose 6 Increase column (GE Healthcare) equilibrated in SEC buffer ...
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bioRxiv - Biochemistry 2020Quote: ... the proteins were buffer exchanged into the appropriate buffer using an Illustra NAP-5 column (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... Nucleic acid contaminants were removed by directly binding CPSF4-YTH onto a 5 ml heparin (GE healthcare) and eluting by a 40 ml step NaCl gradient (150 mM to 2M ...
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bioRxiv - Microbiology 2022Quote: ... Periplasmic proteins were extracted and CusF was purified on a 5 ml StrepTrap HP column (GE healthcare) equilibrated with buffer A (10 mM NaPi ...
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bioRxiv - Immunology 2022Quote: ... 5% Glucose for further purification by cation-exchange chromatography using HiTrap SP High-Performance columns (GE Healthcare), using a gradient elution with 20mM HEPES pH 6.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... Survivin was purified by immobilized metal ion chromatography (IMAC) with a 5 ml HisTrap FF (GE Healthcare) column followed by overnight dialysis in 50 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... The cleaved receptor solution was then loaded onto a 5 mL HiTrap SP HP column (GE Healthcare) using an Akta Start system (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... Size exclusion chromatography was performed on Superdex™ 200 Increase 5/150 GL (GE Healthcare, Chicago, IL).
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bioRxiv - Biochemistry 2019Quote: ... the dialysed WT RAD51 containing sample was loaded onto a 5 ml HiTrap Heparin column (GE Healthcare) and eluted via a linear gradient of 200 mM – 600 mM KCl ...
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bioRxiv - Biochemistry 2019Quote: ... FLAG eluate was loaded onto a 1 mL HisTrap™ High-Performance 5 mL columns (GE Healthcare) using a peristaltic bump and washed with 5 column volumes of 26S NiA buffer (30 mM HEPES pH 7.6 ...
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bioRxiv - Biochemistry 2019Quote: ... Lystate was clarified by centrifugation and loaded onto HisTrap™ High-Performance 5 mL columns (GE Healthcare) using a peristaltic pump ...
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bioRxiv - Biochemistry 2019Quote: ... pH 5.2) for two hours and then loaded onto a MonoS 5/50 GL column (GE Healthcare) equilibrated to the low-salt ion-exchange buffer ...
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bioRxiv - Zoology 2020Quote: ... and 3500 V/2 mA/5 W for 6 h using a Multiphor II system (GE Healthcare). The IEF strip was then equilibrated for 15 min in 5 ml equilibration stock solution (6 M urea ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysates were cleared by centrifugation and loaded onto a 5 ml HisTrap HP column (GE Healthcare). After washing with buffer A (50 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2019Quote: ... and the supernatant loaded onto a freshly charged 5 mL HisTrap FF Ni-affinity column (GE Healthcare) at 1 mL/min at 4 °C (50 mM Tris buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Eluted protein was further purified using an anion exchange column (HiTrap Q HP 5 ml; GE Healthcare) before buffer exchange into PBS and storage at –20°C.
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bioRxiv - Biochemistry 2021Quote: ... Then the mixture was purified by Mono Q 5/50 GL ion-exchange chromatography (GE Healthcare, USA), resulting in a stable nsp7-nsp8-nsp12 complex ...
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bioRxiv - Biochemistry 2020Quote: ... and the cleaved sample was loaded onto a HisWash2 equilibrated 5 ml HisTrap HP column (GE Healthcare). The flow-through was collected as 2 ml fractions ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were diluted to 75 mM NaCl and loaded onto a MonoQ 5/50 GL (GE Healthcare) equilibrated in QO buffer (20 mM Tris pH 8 ...
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bioRxiv - Immunology 2021Quote: ... Supernatant was harvested after 4-5 days and antibody purified on Protein G Sepharose (Abcam, GE Healthcare) following standard protocols ...
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bioRxiv - Immunology 2020Quote: ... Microglia were separated from debris by resuspending the pellet in 5 ml 33% percoll solution (GE Healthcare). Mast cells and macrophages were obtained from the peritoneal cavity by lavage with 5 ml cold PBS + EDTA (2 mM ...
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bioRxiv - Molecular Biology 2019Quote: ... The cleared lysate was filtered (0.8 µM) and applied to a 5 ml GSH column (GE Healthcare) equilibrated in washing buffer (lysis buffer without DNAse and PMSF) ...
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bioRxiv - Biochemistry 2019Quote: ... passed through a 0.2 µm filter and applied to a 5 ml HisTrap column (GE healthcare, USA). The bound protein was eluted with a gradient of buffer B (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Membranes were washed (6×5 min) with TBS-T and proteins were visualized by quimioluminiscence (GE Healthcare Amersham™ ECL™ Western Blotting Detection Reagents ...
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bioRxiv - Biochemistry 2021Quote: ... The soluble fraction was applied to a 5 mL HisTrap HP Ni2+-NTA IMAC column (GE Healthcare) pre-equilibrated with lysis buffer and washed with 50 mM imidazole ...
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bioRxiv - Biophysics 2020Quote: ... and the clarified cell extract was loaded onto a 5-mL HisTrap HP column (GE Life Sciences) equilibrated in HisTrap Buffer A ...
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bioRxiv - Genetics 2019Quote: ... This diluted sample was then loaded onto a 5 mL Hi-Trap Q HP column (GE Healthcare) and eluted over a linear gradient of 150 mM to 1000 mM NaCl with the protein eluting at 225 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... 100μg/ml of streptomycin and 5% fetal calf serum (FCS; HyClone, GE Healthcare, South Logan, UT, U.S.A.) at 37°C in a humidified atmosphere with 5% CO2.
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bioRxiv - Immunology 2020Quote: IgGs were purified from plasma samples using 5-mL HiTrap MabSelect SuRe columns (GE Healthcare Life Sciences). Heat-inactivated plasma was diluted 10-fold with cold PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... the HisTrap FF column was additionally coupled to a HiTrap Heparin HP 5-ml column (GE Healthcare). Both proteins were subjected to TEV protease cleavage at 4 °C overnight and another HisTrap FF 5-ml column purification step ...
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bioRxiv - Molecular Biology 2021Quote: ... Extract was prepared as above and was passed through a 5 mL HisTrap HP column (GE Healthcare). The column was washed with 10 CV of IMAC buffer ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... CirpA4 and CirpA5 were further purified by ion exchange chromatography (Mono Q 5/50 column, GE Healthcare). Homogeneous protein populations eluted in the peak (CirpA3 ...