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Citations for GE Life Sciences :
1301 - 1350 of 2265 citations for 8 9 5 α CHOLESTEN 24 METHYLENE 3 β OL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Protein purification was performed on HisTrap HP 5 ml Ni-based column (Cytiva, former GE Healthcare) using ÄKTA Explorer System (Cytiva ...
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bioRxiv - Biophysics 2022Quote: ... All different DinB variants were purified using a 5 ml Ni2+-Histrap HP column (GE Healthcare) column equilibrated with buffer A and subsequently eluted with buffer B (buffer A supplemented with 1 M imidazole) ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was clarified by centrifugation and applied onto GSTrap FF 5 mL (GE Healthcare, USA) using a buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2022Quote: ... The clarified cell lysate was first injected onto a 5 ml Ni2+-NTA column (GE Healthcare). The complexes were step-eluted with elution buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... 4°C) and the supernatant was passed over a 5 mL HisTrap FF column (GE Healthcare) by an ÄKTApurifier system (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... 4°C) and the supernatant was passed over a 5 mL StrepTrap HP column (GE Healthcare) by an ÄKTApurifier system (GE Healthcare) ...
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bioRxiv - Neuroscience 2022Quote: ... filtered and injected in a Superdex 200 Increase 5/150 GL column (GE Healthcare Life Sciences) using a 50 μL loop ...
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bioRxiv - Microbiology 2022Quote: ... and applied onto a HisTrap HP 5 ml affinity chromatography column (GE Healthcare, Chicago, United States) equilibrated with binding buffer (20 mM sodium phosphate pH 7.4 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The supernatant was loaded onto a 5 mL Ni2+-affinity column (HiTrap chelating column, GE healthcare), preequilibrated with buffer A ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were loaded on a 5 ml HisTrap™HP column (1 mL/min; GE Healthcare) in a binding buffer containing 100 mM tris-HCl pH 8 ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant of the cell lysate was loaded onto a HisTrap column (5 mL-GE Healthcare) and the bound protein was eluted with an imidazole gradient (0-1 M) ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatants were initially applied to a HisTrap FF crude 5 mL (GE Healthcare UK Ltd.), which were pre-equilibrated with the HisTrap binding buffer (20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysate containing the protein of interest was loaded onto a 5 mL HisTrap column (GE Healthcare) equilibrated in 200 mM potassium phosphate ...
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bioRxiv - Biophysics 2023Quote: ... The filtered supernatant was loaded onto a HisTrap FF 5 mL (GE Healthcare, Marlborough, MA, USA) preequilibrated with 50 mM Tris-HCl (pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... then poured onto loaded onto a pre-equilibrated HiTrap DEAE FF 5 ml column (GE Healthcare) into Buffer B (20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted fractions were pooled and loaded onto a 5 ml anionic exchanger (Q-column; GE Healthcare). A salt gradient of 0-1 M KCl was applied at 5 ml/min over 20 min ...
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bioRxiv - Molecular Biology 2023Quote: ... the clarified lysate was added to a 5 ml HisTrap High Performance column (GE Healthcare/Cytiva), washed with 5 column volumes of lysis buffer supplanted with 6 M urea ...
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bioRxiv - Cell Biology 2022Quote: ... Eluted fractions were pooled and loaded onto a 5 ml anionic exchanger (Q- column; GE Healthcare). A salt gradient of 0-1 M was applied over 20 min and the protein was eluted in 5 ml fractions ...
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bioRxiv - Biochemistry 2023Quote: ... The cell extract was applied onto a HisTrap™ FF crude column (5 ml; GE Healthcare) pre-equilibrated with a buffer containing 50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Microbiology 2023Quote: ... then supernatant was loaded onto a 5 mL Ni2+ affinity column (HisTrap HP, GE Life Sciences) pre-equilibrated with resuspension buffer ...
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bioRxiv - Biophysics 2023Quote: ... and loaded into a Mono Q 5/50 GL (GE Healthcare Life Sciences, Chicago, IL, USA) coupled to an Äkta purifier system (GE Healthcare) ...
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bioRxiv - Biochemistry 2023Quote: ... Supernatant was then circulated over 5 mL HiTrap Chelating column (GE Healthcare, Cat# 17-0409-01) that was inoculated with 100mM CoCl2 for ten minutes ...
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bioRxiv - Bioengineering 2023Quote: ... Affinity purification of the protein was achieved using a StrepTrap HP 5 mL column (GE Healthcare) in binding buffer consisting of 100 mM tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... supernatants filtered through 0.45 µm syringe filter and loaded onto His Trap (5 mL, GE Healthcare) equilibrated with 5% HisB buffer (100% HisB ...
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bioRxiv - Biophysics 2024Quote: ... The PEO solutions are additionally filtered using a 5 µm-pore size membrane from GE Healthcare life sciences ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was loaded on a 5 ml HiTrap Q HP column (GE Healthcare, Pittsburgh, PA), and the flow-through was collected and dialyzed against 20 mM HEPES pH 6.8 ...
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bioRxiv - Biochemistry 2024Quote: ... Antibodies were separated from unincorporated fluorophores on desalting columns (NAP-5 or PD-10, GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... ELISA plates were washed 3 times with PBST and 50 µL of sheep anti-mouse IgG-horseradish peroxidase (HRP) conjugated antibody (GE Healthcare) was added at a dilution of 1:3,000 in blocking solution ...
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bioRxiv - Cell Biology 2020Quote: ... were incubated with GST or GST-tagged angulin-1 cytoplasmic region (409-575 aa or 409-570 aa) for 2-3 h at 4°C and further incubated with Glutathione Sepharose 4B beads (GE Healthcare) for 1 h at 4°C ...
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bioRxiv - Microbiology 2021Quote: 30 ml PBS flow-through plasmapheresis filters or peripheral blood diluted in PBS (1:3) were collected in 50 ml tubes and underlaid with Ficoll-Paque (GE Healthcare). PBMC-containing buffy coat was obtained after centrifugation for 30 min at room temperature at 1200 RCF ...
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bioRxiv - Biophysics 2022Quote: ... the volume was reduced to a volume of 400 ml using the ÄktaFlux system with a 3 kDa MWCO cartridge (GE Healthcare). Afterwards the system was used for buffer exchange with 50 mM HEPES ...
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bioRxiv - Cell Biology 2019Quote: Each oligonucleotide contained an amino group at the 3’ end for fluorophore coupling using either Cy5 NHS ester (GE Healthcare, PA15102) or rhodamine NHS ester (ThermoFisher ...
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bioRxiv - Cell Biology 2019Quote: ... Protein containing fractions were concentrated to 3 mL and further purified by size exclusion using a HiPrep 16/60 Sephacryl S-500 HR (GE Healthcare) in Buffer D (20 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2020Quote: ... 5’-FAM-UGUUCUUCUGGAAGUCCAGTT-3’) was separated by gel electrophoresis (20% polyacrylamide gel containing 7 M Urea) and visualized with a Typhoon FLA9500 (GE Healthcare).
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bioRxiv - Molecular Biology 2021Quote: ... The reaction took place for 3 h and the radio labeled kCR probe purified in a Sephadex G25 column (Amersham, GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... Purified 6-His-PI3Kγ catalytic subunit17,18 was used as the soluble analyte and was injected at different concentrations over the immobilized GRK2 in SPR buffer (0.01 M HEPES pH 7.4, 0.15 M NaCl, 3 mM EDTA, 0.005% surfactant P-20, GE Healthcare Life Sciences, Biacore Inc.). Binding experiments were performed at 25°C in SPR buffer with a flow rate of 20 μl/min ...
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bioRxiv - Biophysics 2021Quote: FH1FH2 constructs of Cdc12p (residues 882-1375) and Bni1p (residues 1227-1776) were expressed from pGEX-4T-3 plasmids (GE Healthcare) in BL21(DE3 ...
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bioRxiv - Cell Biology 2020Quote: ... N571 and N442 in frame with N-terminal tag glutathione S-transferase (GST) in pGEX-6P-3 expression plasmids (GE healthcare) were expressed in BL21-CodonPlus-RP E ...
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bioRxiv - Biochemistry 2022Quote: ... The fractions containing the protein of interest were pooled and concentrated to ∼3 mg/mL using a 100kDa MWCO Vivaspin protein concentrator (GE Healthcare). The concentrated protein was subjected to size exclusion chromatography on Superose 6 increase 10/300 column (GE Healthcare).
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bioRxiv - Immunology 2022Quote: ... ELISA plates were afterwards washed 3 times with PBST and 50 μL of anti-mouse IgG-horseradish peroxidase (HRP) conjugated antibody (Cytiva, GE Healthcare) was added at a dilution of 1:3,000 in blocking solution ...
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bioRxiv - Neuroscience 2020Quote: Dynamic PET/CT imaging (2-3h) with arterial blood sampling was performed on Monkey 3 and Monkey 4 using a Discovery MI (GE Healthcare). Each animal had two baseline scans which were separated by one month for Monkey 3 and by one year for Monkey 4 ...
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bioRxiv - Biochemistry 2021Quote: ... Active HAP fractions eluting from 0.2–0.3 M KPi were pooled and separated on a Superose 6 XK 16/70 gel filtration column (130 ml, GE Healthcare) equilibrated with buffer D + 0.1 mM EDTA at 0.15 M KCl ...
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bioRxiv - Neuroscience 2022Quote: ... apposed them to a BAS-SR2040 phosphor screen (Fujifilm) for 3 days and imaged the slides using a phosphorimager (Typhoon FLA 7000; GE Healthcare). We drew ROIs onto the sections with standards using Multigauge software (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... The samples were then applied to IPG strips and separated in the first dimension with the IPGphor 3 system from GE Healthcare according to their isoelectric focusing (20 C ...
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bioRxiv - Microbiology 2019Quote: ... were loaded on 7-cm long immobilized pH gradient (IPG) strips with non-linear (NL) 3-10 pH gradient (GE Healthcare). Isoelectric focusing was carried out in Multiphor II electrophoresis system (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... the His6-tagged 14-3-3ε was allowed to equilibrate with the excess of untagged 14-3-3γ (overnight at 4 °C) and then loaded on a 1 ml HisTrap HP column (GE Healthcare) while the flowthrough fraction containing 14-3-3γ was collected ...
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bioRxiv - Biophysics 2019Quote: ... The reaction was incubated at 37°C for 2-3 hours on an orbital shaker and loaded onto an anion-exchange colum (1 ml DEAE FF, GE Healthcare) equilibrated in 50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Pathology 2019Quote: ... proteins were separated based on their isoelectric point on 18-cm immobilized pH gradient (IPG) gel strips of pH 3-10 (GE Healthcare). Subsequent to overnight rehydration ...
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bioRxiv - Biophysics 2019Quote: ... 50 mM NaCl to a final concentration of ∼3 mg/ml further purified by size-exclusion chromatography using a Sepharose 6 10/300 (GE Healthcare) column equilibrated in solubilization buffer ...
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bioRxiv - Cell Biology 2020Quote: Computerized axial tomography scanning (CT scan) was performed on anesthetized P30 mice [3% of Isoflurane (Isoba Vet)] using an eXplore ista PET-CTscan (GE Healthcare) with the following parameters ...