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Citations for GE Life Sciences :
1351 - 1400 of 2500 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: The HMWP2-Cy2 supernatant was loaded at 1 ml/ min onto a 5 mL HisTrap column (GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Filtered lysate was loaded onto a pre-equilibrated 1 mL or 5 mL HisTrap HP column (GE Healthcare) charged with Ni2+ ...
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bioRxiv - Microbiology 2022Quote: Solubilized porins were loaded onto an anionic-exchange column (Hitrap HQ, 5 mL, GE Healthcare Life Science, France) and subjected to detergent exchange with 0.1 M MES buffer pH 6.5 complemented with 0.1% lauryldimethylamine-oxide (LDAO ...
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bioRxiv - Biophysics 2022Quote: ... The clarified supernatant was then passed over a Ni-NTA His-Trap FF 5 ml column (GE Healthcare) which was pre-equilibrated with loading buffer composed of 25 mM HEPES pH 7.0 ...
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bioRxiv - Microbiology 2022Quote: ... The top fractions after IMAC were pooled and desalted using a 5 ml HiTrap Desalting column (GE Healthcare) equilibrated with 25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was added to 5 mL of equilibrated Glutathione Sepharose 4B resin (GE Healthcare Bio-sciences AB). The beads were washed in a column 3 times with 50 mL of Buffer C (10 mM phosphate buffer pH 7.4 ...
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bioRxiv - Biophysics 2022Quote: ... the α-Syn solution was subjected to anion exchange column (AEC) (HiTrap Q Hp 5 ml, GE healthcare) followed by size exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was then applied onto a Hi Trap affinity (5 ml) (Ni2+) column (GE Healthcare Life Sciences) The column was washed with 10 column volumes of buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... Dried membranes were scanned using maximum quality and resolution settings with an Amersham Typhoon 5 imager (GE Healthcare) outfitted with 785 nm and 685 nm lasers and IRlong 825BP30 and IRShort 720BP20 filters ...
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bioRxiv - Microbiology 2020Quote: ... Beads were isolated from the suspension by centrifugation (500 x g, 5 minutes) through Percoll Plus (GE Healthcare) in a 50 mL conical tube ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was filtered and loaded onto a 5 mL HisTrap HP column (GE Life Sciences, now Cytiva), followed by washing [0.05 M Tris pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... Cleaved DEPTOR protein was then collected and applied to a 5 mL HiTrap Heparin HP column (GE Healthcare) equilibrated with HEP-A buffer (50 mM HEPES pH 8 ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM DTT) and then loaded on a HiTrap Heparin column (CV, column volume 5 ml; GE Healthcare), equilibrated with buffer A ...
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bioRxiv - Immunology 2022Quote: ... 5% glucose) and the proteins purified by affinity chromatography using a HiTrap Heparin High-Performance column (GE Healthcare) and gradient elution (0-100% NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were grown at 37°C and 5% CO2 in high-glucose (4.5 g/L) DMEM (GE Healthcare) supplemented with 10% fetal calf serum (Biochrom ...
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bioRxiv - Biochemistry 2020Quote: ... and the crude lysates were applied to 5 ml HiTrap Capto Q column (GE Healthcare Life Sciences, USA) equilibrated with 5 volumes of buffer A (20 mM Tris (pH 8.0) ...
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bioRxiv - Molecular Biology 2021Quote: ... the supernatant containing complexes of Panx and LC8 was loaded onto 5 ml HisTrap Fastflow column (GE Healthcare). After extensive washing with Binding buffer ...
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bioRxiv - Pathology 2021Quote: ... diluted five-fold with extraction buffer and applied to a 5 ml HisTrap column (GE Healthcare, Chicago, IL) equilibrated with extraction buffer ...
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bioRxiv - Biophysics 2020Quote: ... The EcA2 used for these experiments were previously desalted in a 5-mL HiTrap Desalting (GE Healthcare, USA) and eluted in 10 mM sodium phosphate at pH 7.0 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The lysate obtained was loaded onto the pre-equilibrated nickel affinity column (HisTrap FF, 5 mL, GE Healthcare) with loading buffer (50 mM KPi ...
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bioRxiv - Biophysics 2020Quote: ... Fractions containing Pol I were then loaded onto a 5-mM HiTrap Heparin HP column (GE Life Sciences) equilibrated in Heparin BufferA(50mMTris-HCl ...
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bioRxiv - Cell Biology 2021Quote: Approximately 5 μg of GST or GST-tagged protein fragments were incubated in Glutathione-Sepharose beads (GE Healthcare) in GST-lysis buffer at 4°C overnight ...
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bioRxiv - Microbiology 2023Quote: ... proteins were separated by immobilized-metal affinity chromatography (IMAC) on a 5 mL HisTrap HP column (GE Healthcare), previously washed with ddH2O and equilibrated with buffer B (25 mM HEPES/NaOH pH 7.5 ...
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bioRxiv - Neuroscience 2023Quote: ... ATXN1 blots were then placed in 5% milk PBST plus 1:2500 rabbit specific HRP antibodies (GE Healthcare) while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare ...
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bioRxiv - Molecular Biology 2022Quote: ... Chemiluminescence was detected after 5 min of incubation with ECL™ Select Western Blotting Detection Reagent (GE Healthcare) in a ChemiDoc chemiluminescence detector (BioRad) ...
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bioRxiv - Biochemistry 2024Quote: ... Removal of nucleic acid contaminants was performed using 1 ml or 5 ml HiTrap Heparin column (GE Healthcare) and eluted using a NaCl gradient from 50 mM to 1 M ...
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bioRxiv - Biochemistry 2024Quote: ... The Spt4/5 heterodimer was further purified by size exclusion chromatography (Superdex 75 10/300 GL, GE Healthcare) through isocratic elution in low salt buffer ...
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bioRxiv - Cell Biology 2024Quote: ... We then applied the supernatant onto two tandem 5 mL HiTrap Q HP anion exchange columns (GE Healthcare) pre-equilibrated with 20 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2024Quote: ... blots were incubated at room temperature for 5 min in the dark with ECL detection reagent (GE Healthcare), made up in accordance with manufacturers’ instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Then the solution was injected onto a prewashed SP Sepharose HiTrap column (5 ml, GE Healthcare, Chicago, IL). The column was washed with 20 CV of Buffer D (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Aurora A protein was thawed and buffer exchanged in the experimental buffer using NAP-5 Columns (GE Healthcare). Experiments typically involved titrating 25 µM of protein in the sample cell with 300 µM of compound in the syringe ...
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bioRxiv - Biochemistry 2023Quote: The ammonium sulfate treated hMDH preps were loaded onto a 5 mL HiTrap Butyl HP column (GE Healthcare). An inverse linear gradient of ammonium sulfate from 1.5 M to 0 M was used to purify hMDH where it eluted at a conductivity of 135 mS/cm ...
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bioRxiv - Biophysics 2023Quote: ... centrifuged and applied to a cation exchange chromatography column (5 mL HiTrap SP HP, GE Healthcare Life Science) equilibrated with 25 mM sodium phosphate ...
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bioRxiv - Biochemistry 2023Quote: ... a second nickel-affinity chromatography was performed (His Trap, 5 mL, GE Healthcare; equilibrated in 10% HisB buffer). Sample was loaded and unbound protein further washed out with 10% HisB ...
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bioRxiv - Microbiology 2022Quote: ... Strep-tagged proteins were affinity-purified on an ÄKTA Purifier using 5 ml StrepTrap HP columns (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant Sau-MenD was purified at room temperature by IMAC with 5 mL HisTrap HP columns (GE Healthcare) and an imidazole gradient of 20–500 mM over 50 mL ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was added to a 5 mL Ni-Sepharose fast-flow resin (GE Healthcare Life Sciences) pre-equilibrated with buffer A at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Samples (50 μl) were loaded on a Superdex 200 Increase 5/150 column (GE Healthcare, Chicago, Illinois, USA) pre-equilibrated with a 20 mM Tris-HCl buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... The shRNA constructs targeting mouse ezrin (5’-ATTTCCTTGTTATAATCTCCG-3’) in a pLKO-puro.1 vector from GE Healthcare and described in27 ...
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bioRxiv - Biophysics 2022Quote: ... with a cut-off of 5 kDa and injected into HiLoad Superdex 200 prep grade chromatography (GE Healthcare) equilibrated with buffer C (20 mM Hepes-NaOH ...
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bioRxiv - Microbiology 2023Quote: ... The cell-free lysate was applied to a 5 ml His Trap™ HP column (GE Healthcare, USA) equilibrated in the same buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then transferred to a 5 mL tube containing 300 μL CaM-Sepharose resin (GE Healthcare) equilibrated in 50 mM HEPES (pH 7.2 ...
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bioRxiv - Cell Biology 2024Quote: Time-lapse images were collected at 37°C with 5% CO2 using a DeltaVision ELITE microscope (Applied Precision). We used an apochromatic 100x objective lens (Olympus ...
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bioRxiv - Cell Biology 2024Quote: ... and subsequently loaded onto a HiTrap SP (CEH-37)/Q (POT proteins) HP 5 mL column (GE Healthcare). An appropriate salt gradient was applied using 20 mM HEPES pH 7.5 buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... passed through a 0.22 μm filter and applied to a 5 mL HiTrap Q HP column (GE Healthcare). The column was washed with 2 CV of buffer Q ...
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bioRxiv - Biochemistry 2024Quote: ... RRM2 (>5 mg mL-1) was injected onto a 24 mL Superdex 75 size exclusion column (GE Healthcare) at a flow rate of 1 mL min-1 ...
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bioRxiv - Biochemistry 2024Quote: ... TEV protease and non-cleaved protein was removed by IMAC (HisTrap FF Crude column, 5 ml, GE Healthcare) with a linear gradient of imidazole (0-500 mM) ...
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bioRxiv - Biochemistry 2024Quote: ... The eluents were pooled and directly loaded onto HiTrap Q HP 5 mL anion exchange column (GE Healthcare) pre-equilibrated with buffer D [50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Biochemistry 2024Quote: ... The dialyzed solution was then loaded on a 5 ml HiTrap SP FF column (GE Healthcare, Pittsburgh, PA) and eluted using a linear gradient of NaCl (20-500 mM) ...
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bioRxiv - Biochemistry 2024Quote: ... Excess HaloTag ligand was then removed by purification on desalting NAP-5 or PD-10 columns (GE Healthcare). To generate the HRP-his6Halo-hPKR-N conjugate ...