Labshake search
Citations for GE Life Sciences :
1601 - 1650 of 2500 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... The supernatant of the cell lysate was incubated overnight at 4°C on a wheel with Glutathione Sepharose 4B resin (GSTrap 4B, GE Healthcare). After several washes ...
-
bioRxiv - Microbiology 2023Quote: ... The clarified lysate was incubated (overnight, at 4 °C, with gentle agitation) with 1 ml bed volume of GSH-Sepharose (GE Healthcare). Next ...
-
bioRxiv - Biochemistry 2023Quote: ... the N-terminal His tag was cleaved by overnight dialysis with TEV protease at 4 °C and purified by size exclusion chromatography (SEC) (HiLoad Superdex-200 16/600 column, GE Healthcare) in CHIP storage buffer (50 mM HEPES ...
-
bioRxiv - Cell Biology 2023Quote: ... actin Ac-74 or Pol II S5P) overnight at +4°C and immune-complexes were collected with 50 μl of protein A sepharose (GE Healthcare) at +4°C for two hours on rotation ...
-
bioRxiv - Molecular Biology 2023Quote: ... CpxA31-163-GST was purified at 4°C using a protocol modified from the GST Gene Fusion System Handbook (GE Healthcare). Briefly ...
-
bioRxiv - Microbiology 2023Quote: ... HA-UL47-containing protein complexes were immunoprecipitated by incubating extracts with HA-specific antibodies and protein G-coupled sepharose beads (4 Fast Flow, GE Healthcare). The sepharose-bound proteins were analyzed by standard immunoblotting for the presence of HA-UL47 ...
-
bioRxiv - Cell Biology 2023Quote: ... hDrp1 was eluted by cleavage from the affinity resin using 30 mM DTT at pH 8 for 48 hours at 4 °C and further purified using anion exchange chromatography (HiTrap Q FF, GE Healthcare) at pH 8 ...
-
bioRxiv - Biophysics 2023Quote: ... protein samples were purified by size-exclusion chromatography at 4°C using a Superose 6 10/300 GL column (GE Healthcare), equilibrated with a buffer containing 0.03% (w/v ...
-
bioRxiv - Cell Biology 2023Quote: ... and indicated prey proteins at a 5X molar excess to tepsin (mATG8 and/or AP-4 β4 appendage) were incubated with glutathione sepharose resin (GE Healthcare) for 2 hours at 4°C in 20 mM HEPES (pH 7.5) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Wells were washed twice with room temperature PBS and then 4 fields of view per well imaged on an InCell 6000 (GE Healthcare). Images were segmented with two custom Cellpose[45] models to segment the nucleus (from DAPI channel ...
-
bioRxiv - Neuroscience 2024Quote: ... The protein bands were detected by incubating the membrane with primary antibodies (see below) overnight at +4°C and horse radish peroxidase-conjugated secondary antibodies (1:5000, NA934 or NA931, GE Healthcare) for 1 h at RT ...
-
bioRxiv - Immunology 2024Quote: ... An affinity matrix was prepared by coupling 50 mg of His-tagged GST-RAP to 20 ml of NHS-activated Sepharose 4 Fast Flow (GE Healthcare), according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2024Quote: ... The protein was concentrated using a 100 kDa filter to approximately 1 mL and further purified by size-exclusion chromatography at 4°C using a Superose 6 10/300 GL column (GE Healthcare) equilibrated with buffer containing 0.06% digitonin ...
-
bioRxiv - Developmental Biology 2024Quote: ... The GST-fused Ccrk protein was expressed in Escherichia coli strain BL21 (DE3) and purified using glutathione Sepharose 4 B (GE Healthcare). An antibody against Ccrk was obtained by immunizing guinea pigs with purified GST-fused Ccrk.
-
bioRxiv - Microbiology 2024Quote: ... The 6ξ-His tag was cleaved for 16 h at 4 °C using 1U of thrombin per 100 µg of total protein (GE Healthcare). The cleaved proteins were then passed through a Ni-NTA column to remove the cleaved 6ξ-His tag and any uncleaved protein ...
-
bioRxiv - Plant Biology 2024Quote: ... The mixture was centrifuged at 2,000 g and 4 °C for 10 min and eventually filtered with 0.43 μm organic phase medium (GE Healthcare, 6789-0404). The column was a 100×2 mm Phenomenex Luna 3 μm NH2 (Catalog No. ...
-
bioRxiv - Plant Biology 2024Quote: ... 2D gel electrophoresis was performed as described previously (Bhattacharya et al., 2019) using 11 cm Immobiline DryStrips pH 4-7 (GE Healthcare) in Ettan IPGphor3 IEF system (GEHealthcare) ...
-
bioRxiv - Cell Biology 2024Quote: ... The membrane was incubated first with primary Abs for 24 hours at 4°C and then with HRP–conjugated secondary Abs (GE Healthcare) for 1 hour at room temperature ...
-
bioRxiv - Cell Biology 2024Quote: ... the protein debris was removed by centrifugation (30000×g, 20 min at 4 °C) and the supernatants were applied to His-Trap chelating column (GE Healthcare), washed by refolding buffer and eluted with the same buffer containing 30 mM imidazole ...
-
bioRxiv - Biochemistry 2024Quote: ... pH 7.4) using a HiLoad 16/60 Superdex 200 pg gel filtration column operated on an ÄKTA liquid chromatography system (GE Healthcare).
-
bioRxiv - Pathology 2024Quote: ... 125 µg of biotinylated PAI-1WT (containing the 4-stabilizing substitutions (20)) was bound to 125 µL of streptavidin-sepharose beads (GE Healthcare). PAI-1-streptavidin-sepharose beads were added to 500 µL of lung supernatant and incubated at 37°C for 1 hour with gentle agitation ...
-
bioRxiv - Immunology 2024Quote: ... Cells were filtered through 70 µm cell strainers and then centrifuged at 300xg for 10 minutes at 4°C before being resuspended in 22% Percoll (GE Healthcare) and centrifuged at 950xg for 20 minutes (acceleration=4 ...
-
bioRxiv - Immunology 2024Quote: ... Samples were centrifuged at 220xg for 10 min at 4°C and then resuspended in a 22% Percoll solution (GE Healthcare) with a PBS layer floated on top ...
-
bioRxiv - Systems Biology 2024Quote: ... overnight at 4°C followed by a 1/10.000 dilution of anti-mouse IgG HRP-linked whole secondary antibody (GE Healthcare NA931), during 1 hour at room temperature ...
-
bioRxiv - Biochemistry 2024Quote: ... Gels were run for 45 min at 150 V at 4°C before detecting the HEX-label using a Typhoon FLA 9500 instrument (GE Healthcare) at 532 nm wavelength and BPG1 emission filter ...
-
bioRxiv - Biochemistry 2024Quote: ... and subsequently concentrated by Amicon Ultra-4 centrifugal filter (MWCO 100 kDa) before being injected into a Superose 6 Column (GE Healthcare). The size exclusion chromatography (SEC ...
-
bioRxiv - Biochemistry 2024Quote: ... Lysates were clarified by centrifugation at 50,000⍰×⍰g for 1⍰h at 4⍰°C and the supernatant was used for affinity purification on HisTrap FF columns (GE Healthcare) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... Cellular debris was pelleted by centrifugation for 20 min (18000 × g at 4°C) and GST and MBP fusion proteins were purified using glutathione- Sepharose (GE Healthcare) or amylose (New England Biolabs ...
-
bioRxiv - Biochemistry 2024Quote: ... incubated at 4 °C for 30 min and purified using gel filtration (Superdex 200 Increase 10/300 GL) columns (GE Healthcare).
-
bioRxiv - Cell Biology 2024Quote: ... Between 5-20 μg of total protein per sample were resolved by SDS-PAGE (4-20% gels) and transferred to Amersham Hybond P 0.45 PVDF blotting membranes (GE Healthcare, 10600023). Membranes were blocked overnight at 4°C in 5% skim milk powder or 5% BSA in PBS 0.1% Tween when working with phospho-specific antibodies ...
-
bioRxiv - Biochemistry 2024Quote: Wild-type BbZIP and the H149A/H151A variant were purified as described above and the N-terminal His6-tag was removed by incubation with thrombin overnight at 4°C before it was loaded to a Superdex Increase 200 column (GE Healthcare) equilibrated with a Cd-free solution containing 10 mM Hepes (pH 7.3) ...
-
bioRxiv - Developmental Biology 2024Quote: ... Normalized protein samples were resolved on 4-20% tris-glycine gels and transferred to Amersham Hybond P 0.45 PVDF blotting membranes (GE Healthcare, 10600023). Membranes were blocked for 1h at room temperature in PBS-T with 5% milk ...
-
bioRxiv - Plant Biology 2020Quote: ... the supernatant was collected and incubated for 5 min on 1 mL Ni Sepharose® 6Fast Flow (GE Healthcare) equilibrated with 10 mL binding buffer ...
-
bioRxiv - Microbiology 2020Quote: ... coli BL21(DE3) and were isolated under native purification conditions using a 5 HisTrap High Performance Column (GE Healthcare) with imidazole gradient (10-250 mM ...
-
bioRxiv - Biophysics 2021Quote: ... 10 μM ZnCl2 and 1 mM DTT) and loaded into a HiTrap Heparin HP 5 ml column (GE Healthcare) equilibrated with 6.25% of buffer HepB (buffer HepA supplemented with 2 M NaCl) ...
-
bioRxiv - Neuroscience 2021Quote: ... filtered through 0.45 μm cut-off filters and loaded onto a HiTrap SP HP 5 ml column (GE Healthcare) for cation exchange ...
-
bioRxiv - Cell Biology 2020Quote: ... Antibody dilutions in 5% milk/PBST: anti-MBP HRP conjugated, 1:50,000 (NEB, and anti-GST HRP conjugates, 1:2,000 (GE Healthcare,). Scanned western blot films were quantified using the gel analysis tool in ImageJ.
-
bioRxiv - Molecular Biology 2021Quote: ... The protein fraction eluted with 250 mM imidazole was purified further by MonoQ 5/50 GL column (GE Healthcare) at pH 8.0 ...
-
bioRxiv - Cell Biology 2022Quote: ... Filtered supernatant was loaded into a 5 mL Histrap column (Cytiva) connected to an FPLC AKTAxpress system (GE Healthcare). After affinity binding ...
-
bioRxiv - Neuroscience 2021Quote: ... They were maintained within 37°C incubators at 5% CO2 in DMEM + high glucose (GE Healthcare Bio-Science, SH30022FS) supplemented with 9.09% fetal bovine serum (50 mL added to 500 mL DMEM ...
-
bioRxiv - Molecular Biology 2020Quote: ... Sucrose gradient fractions containing ODA complexes were further purified over a MonoQ 5/50 anion exchange column (GE Healthcare) to separate out other axonemal dynein species ...
-
bioRxiv - Biochemistry 2020Quote: ... purification of the protein was performed with a histidine affinity column (HisTrap FF crude 5 ml, GE Healthcare Amersham) taking advantage of the natural His present in the octapeptide repeat region of PrP ...
-
bioRxiv - Immunology 2020Quote: ... Dilution series of TBK1 or IRF-3 (1.25, 2.5, 5, 10, 20 μM) in 1× HBS-EP+ buffer (GE Healthcare) were injected over the sensor chip at a flow rate of 30 μL/min ...
-
bioRxiv - Biochemistry 2021Quote: ... The cleaved protein was then passed over a HisTrap™ FF Ni2+ column (5 ml/min, GE Life Sciences) and washed with Buffer B and the cleaved topo VI collected in the flow through ...
-
bioRxiv - Biochemistry 2021Quote: AsLMO10A and AsLPMO10B were purified by anion exchange chromatography using a 5 mL HiTrap DEAE FF column (GE Healthcare) followed by hydrophobic interaction chromatography (HIC ...
-
bioRxiv - Biochemistry 2021Quote: ... The protein was additionally purified by ion-exchange chromatography on a “Mono S 5/50 GL” column (GE healthcare) at 0-100% B gradient over 15 column volumes at 0.5 ml/min flow rate using 20 mM sodium phosphate buffer (pH 6.0 at 25 °C ...
-
bioRxiv - Biochemistry 2020Quote: ... and 1 mM DTT and loaded directly onto a 5 ml HiTrap SP Sepharose fast flow column (GE Healthcare). Bound Nup84-Nup133 was eluted from the column using a 15 column volume (CV ...
-
bioRxiv - Biochemistry 2020Quote: ... The sample was diluted to 70 mM NaCl and injected into an HiTrap Heparin HP 5 ml (GE Healthcare) column equilibrated with 20 mM HEPES pH 7.9 ...
-
bioRxiv - Biochemistry 2020Quote: Soluble protein was manually applied at room temperature to a 5 mL HiTrapQ anion exchange column (GE Healthcare # 29051325) that had been pre-equilibrated in buffer B ...
-
bioRxiv - Biochemistry 2021Quote: The filtered lysates were subjected to IMAC using a His-Trap 5 mL Nickel column (GE Healthcare Life Sciences) on an ÄKTA Pure system (GE Healthcare Life Sciences) ...