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Citations for GE Life Sciences :
1301 - 1350 of 3780 citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... Fusion proteins were first immobilized at a coupling density of ∽1000 response units (RU) on a series S sensor chip protein A (GE Healthcare) in PBS and 0.02% (w/v ...
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bioRxiv - Microbiology 2021Quote: ... The liposomes were then purified by gel filtration using a Hi Prep 16/60 Sephacryl S-300 HR column (GE Healthcare) in 150 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... iMAC samples were thawed on ice and loaded into a purification column packed with 10 mL of Sephacryl S-100 (GE Healthcare) equilibrated with 25 mM Tris-HCl ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were treated with RNase I (5U/OD260) and ribosome-protected fragments were isolated via centrifugation through Microspin S-400 HR columns (GE Healthcare) and purified using the RNA Clean and Concentrator kit (Zymo Research) ...
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bioRxiv - Biophysics 2021Quote: ... The three encapsulins used in this study were purified via size exclusion chromatography (SEC) using a HiPrep 26/60 Sephacryl S-500 HR column (GE Healthcare) equilibrated with 50 mM HEPES pH 7.4 ...
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bioRxiv - Biophysics 2021Quote: ... Fractions containing the protein of interest were pooled together and loaded onto a pre-equilibrated size exclusion column (HiPrep 16/60 Sephacryl S-300, GE Healthcare). The protein was then eluted using HSU buffer and the protein fractions were used immediately or frozen in liquid nitrogen and stored at −80°C.
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bioRxiv - Biophysics 2020Quote: ... and non-deamidated (pure N373 wildtype) GII.4 P dimer species was achieved by cation exchange chromatography using a 6 ml Resource S column (GE Healthcare) at 6 °C ...
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bioRxiv - Cell Biology 2021Quote: ... The sample was loaded onto either a 1-ml Mono Q (ParAF proteins and ParBF1-42-mCherry-EcoRIE111Q) or Mono S (other ParBF proteins) 5/50 GL ion exchange column (GE Healthcare) pre-equilibrated with Mono Q/S-buffer containing a NaCl concentration to match the conductivity of the sample ...
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bioRxiv - Biochemistry 2022Quote: ... Further polishing of the complex prior SEC and after tag cleavage with PreScission protease was carried out by loading onto a cation-exchange (CE) Resource-S column (GE Healthcare) equilibrated in 10mM HEPES (pH 7.5) ...
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bioRxiv - Immunology 2022Quote: ... and by size-exclusion chromatography using Superdex 200 (RBD and NTD) or Superose 6 (S-2P and ferritin) column (GE Healthcare) in 10 mM Tris pH=8 ...
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bioRxiv - Bioengineering 2022Quote: ... were immobilized on individual flow cells at 5 μl/min using a streptavidin-coated sensor chip (Series S Sensor chip SA, GE Healthcare). Three-fold serial dilutions of recombinant DLL4 proteins (N-EGF5 ...
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bioRxiv - Immunology 2020Quote: ... Conditioned medium was clarified by centrifugation and protein was sequentially purified by a HisTrap HP column and a HiPrep 16/60 Sephacryl S-300 HR size exclusion chromatography (SEC) column (both from GE Healthcare). Purified protein was first analyzed by Native-PAGE and Western blot ...
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bioRxiv - Immunology 2021Quote: The purification of S protein from cell broth was performed using on AKTA Pilot 600R system (GE Healthcare, Little Chalfont, UK). After harvesting ...
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bioRxiv - Biochemistry 2022Quote: ... The N-terminal His6 tag was removed by incubating the purified proteins with ∼3% (w/w) thrombin (4°C, overnight) and then subjected to SEC using a Superdex S-200 16/60 column (GE Healthcare) pre-equilibrated with the running buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Biophysics 2019Quote: ... and a final purification step was done for the Hbs expressed from the pHE2 and pHE9 plasmids using a S Sepharose cation exchange column (GE healthcare) to eliminate Hbs with uncleaved initiator Met residues ...
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bioRxiv - Microbiology 2019Quote: ... the His6-tag was removed using TEV protease and the protein was subjected to size exclusion chromatography (HiPrep 26/60 Sephacryl S-200; GE Healthcare) using a buffer consisting of 40 mM HEPES pH 7.4 and 150 mM NaCl ...
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bioRxiv - Biochemistry 2019Quote: ... the beads were centrifuged and the supernatant was run over a HiPrep Sephacryl 16/60 S-100 HR column (GE Healthcare) in Crystal Buffer 1 (10 mM Tris-HCl pH 8.3 ...
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bioRxiv - Immunology 2019Quote: Phl p 5 mannan neoglycoconjugates (P5-MN) were subjected to size exclusion chromatography using a 16/60 Sephacryl S-300 HR column (GE Healthcare) (Suppl ...
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bioRxiv - Cell Biology 2019Quote: ... Southern analysis was performed by separating DNA fragments in 1% agarose/1X TBE gels using pulse-field electrophoresis with a 5-45 s ramp and blotted onto Hybond-XL membranes (GE Healthcare) with alkaline transfer.
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bioRxiv - Biophysics 2020Quote: ... Cleaved protein was eluted from the beads and further purified on a cation exchange column (Mono S 10/100, GE Healthcare) in MES pH 6.5 ...
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bioRxiv - Synthetic Biology 2020Quote: ... The gel filtration was performed with a HiPrep 16/60 Sephacryl S-100 column on an AKTA chromatography system (GE Healthcare), and the size of the proteins in each eluted fraction were verified by SDS-PAGE.
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bioRxiv - Cell Biology 2020Quote: Frozen aliquots of SARS-CoV-2 Spike trimer (Wuhan) and D614G mutant S trimer were thawed and purified by size exclusion chromatography (Superose 6 Increase 10/300, GE Healthcare) using 2 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2020Quote: ... FP3 or FP4 from the Mono S column were pooled and further purified by SEC performed on a 24 mL Superdex 200 increase column (GE Healthcare) at 4 °C with a flow rate of 0.25 mL/min in a buffer containing 20 mM HEPES ...
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bioRxiv - Cell Biology 2020Quote: ... All capture surfaces were regenerated with one 30-s pulse of 10 mM glycine–HCl (pH 1.5, (GE Healthcare, Marlborough, MA). Kinetic parameters were obtained by globally fitting the data to a 1:1 binding model with mass transport limitation using Scrubber 2.0c Evaluation Software ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was eluted with 240 mM imidazole and further purified over a Superdex S-75 gel filtration column (GE Healthcare) equilibrated in 150 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: For crystallization trials the ATAD2 BRD was further purified by gel-filtration using a HiPrep 16/60 Sephacryl S-100 High Resolution column (GE Healthcare). The purified ATAD2 BRD 966-1112 ...
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bioRxiv - Pathology 2021Quote: ... and 3500 V for 28 kVh (linear) with a Hoefer™ IEF 100 (M/s. GE Healthcare Life Sciences, Amersham, UK) at 20 °C ...
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bioRxiv - Immunology 2020Quote: ... Purified mAbs were first immobilized at coupling densities of 800-1200 response units (RU) on a series S sensor chip protein A (GE Healthcare) in PBS and 0.02% sodium azide buffer ...
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bioRxiv - Microbiology 2020Quote: ... the recovered recombinant proteins were further purified by size exclusion chromatography using a Sephacryl S-400 16/60 column (GE Healthcare). Fractions containing the protein were pulled ...
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bioRxiv - Microbiology 2021Quote: Purified TlpALBD was immobilized on a CM5 series S sensor chip and binding affinities tested using a BIAcoreTM S200 instrument (GE Healthcare) as described (Day et al. ...
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bioRxiv - Immunology 2021Quote: ... and ACE2(HH:NN)-Fc constructs were captured on flow cell 2 of a Series S Protein A sensor chip (GE Healthcare – 29127555) to a density of 500 RU using a Biacore 8K instrument (GE Healthcare) ...
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bioRxiv - Bioengineering 2021Quote: ... Sonications (10-ms bursts applied at 2 Hz for 100 s) were applied coincident with an injection of the MB ultrasound contrast agent Optison (GE Healthcare, Little Chalfont ...
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bioRxiv - Biophysics 2022Quote: ... Aggregates were removed by centrifugation (20 min, 48000xg, 4° C) and the protein solution loaded onto a 6 ml Resource S column (GE Healthcare) equilibrated in 20 mM MOPS-NaOH pH 7.0 ...
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bioRxiv - Cell Biology 2022Quote: ... epifluorescence microscopy as well as time-lapse experiments (500 ms time-lapse for 60 s) were performed on a Deltavision OMX V4 microscope (GE Healthcare) equipped with three water-cooled PCO edges CMOS cameras ...
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bioRxiv - Microbiology 2022Quote: ... Size exclusion chromatography was performed in 20 °C ÄKTA purifier system (Amersham) and a prepacked Hi-Prep 16/60 Sephacryl S-200 high-resolution column (GE Healthcare). Flow rate was 0.5 ml/min ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and resuspended in 10 mL 1X S basal buffer for COPAS biosorter analysis and imaging on a GE InCell analyzer 2200 (GE Healthcare). See Supplementary Methods for full details.
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bioRxiv - Molecular Biology 2022Quote: ... The purified MUS81-EME1 was subjected to cation-exchange chromatography (CEC) with a Mono S 4.6/100 PE column (GE Life Sciences) on ÄKTA explorer FPLC system ...
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bioRxiv - Biochemistry 2023Quote: ... The flow through (containing soluble free histones) was filtered with 0.22 μ filters and loaded on a cation exchange Resource S column (GE Healthcare) equilibrated with the dilution buffer EB5_1 (50 mM Tris-HCl pH8) ...
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bioRxiv - Molecular Biology 2024Quote: ... The final solution was then purified to greater than 95 % purity over a Sephadex S-300HR gel filtration column (GE Healthcare) and buffer exchanged at the same time into binding buffer (20 mM phosphate buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... The final solution was then purified to greater than 95 % purity over a Sephadex S-300HR gel filtration column (GE Healthcare) and buffer exchanged at the same time into binding buffer (20 mM phosphate buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Concentrated FhES (10 mg total protein) was fractionated (2 mL/fraction) by SEC on a Sephacryl S-200 HR 26/60 column (GE Healthcare) connected to an AKTA Start automated liquid chromatography system (GE Healthcare) ...
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bioRxiv - Cell Biology 2024Quote: ... We pooled the α-syn-containing fractions and further purified the protein by size-exclusion chromatography on a HiPrep Sephacryl S-200 HR 16/60 gel filtration column (GE Healthcare) in 50 mM NH4Ac pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... of SOD1 is further purified by size-exclusion chromatography (SEC) using a custom made gel filtration column (dimensions: id 1.2×100cm) packed with SephacrylTM S-200 (GE Healthcare, USA) in order to to isolate the dimeric protein fraction in TBS buffer ...
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bioRxiv - Biochemistry 2023Quote: ... monomeric actin was separated from oligomeric species by passing it through a HiPrep 26/60 Sephacryl S-200 HR size-exclusion column (GE Healthcare) equilibrated in G-buffer ...
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bioRxiv - Biochemistry 2023Quote: ... and separated from high-molecular weight contaminants by size-exclusion chromatography using a Sephacryl S-200 HR column (Cytiva/GE Healthcare). The purified CapZ was flash-frozen in liquid N2 and stored at −80 °C in a buffer containing 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... The refolded WT- and E111V-GCAP1 variants were purified by size exclusion chromatography (SEC, HiPrep 26/60 Sephacryl S-200 HR, GE Healthcare) followed by anion exchange chromatography (AEC ...
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bioRxiv - Immunology 2023Quote: SPR measurements were performed using a Biacore T200 (GE Health-care) instrument docked with an S CM5 series sensor chip (GE Healthcare), as described (Murugan et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... Cat#47410-26615 or Cat#47410-07595) or with a FUSION Solo S imaging system (VILBER) using ECL reagents (GE Healthcare). Quantification was performed via densitometry using ImageJ40 ...
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bioRxiv - Molecular Biology 2023Quote: ... The fusion protein was eluted using binding buffer with 250 mM imidazole and then further purified using size-exclusion chromatography on a Hi load 16/60 Sephacryl S- 100 column (GE Healthcare) in the binding buffer with pH adjusted to 7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... To remove any residual impurities final step of size-exclusion chromatography on a Hi load 16/60 Sephacryl S-100 column (GE Healthcare) in the binding buffer with pH adjusted to 7.4 was employed ...