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Citations for GE Life Sciences :
1301 - 1350 of 3820 citations for 6 Pteridinepropanoic acid 2 4 diamino α 4 methoxycarbonyl phenyl α 2 propyn 1 yl methyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The buffer exchange of fragments produced by limited proteolysis (2 mg/mL) was performed using SEC on a Superdex 200 Increase 3.2/300 column (GE Healthcare Life Science) with 100 mM NH4CH3CO2 at pH 6.8 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 49A were generated by inserting the BamHI–EcoRI PCR fragments amplified from pEGFP plasmids into the BamHI–EcoRI sites of the N-terminal GST-fused protein expression vector pGEX-6P-2 (GE Healthcare, US). Recombinant proteins were obtained as described in Supporting Methods 1 ...
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bioRxiv - Biochemistry 2019Quote: ... 100 μL of purified protein/complex at 2-5 mg/mL was injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in a buffer containing 20 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Genomics 2019Quote: ... and whole genome amplification was achieved by multiple displacement amplification with Phi29 DNA polymerase and the Illustra GenomiPhi v.2 kit (GE Healthcare, 25660032). Amplified genomic DNA (70 ng ...
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bioRxiv - Physiology 2019Quote: ... 20 islets per well (n = 3 biological replicates per group) were transfected with control (non-targeting #2) siRNA or mouse Brd4 SMARTpool siRNA (Dharmacon GE Life Sciences) at 100 nM each with lipofectamine 3000 according to the product instructions ...
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bioRxiv - Immunology 2020Quote: SEC-purified SARS-CoV-2 RBD-SD1 was immobilized to a Ni-NTA sensor chip in a Biacore X100 (GE Life Sciences) to a response level of ~500 RUs ...
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bioRxiv - Synthetic Biology 2021Quote: ... that had been pre-equilibrated with 1.2 column volumes of sizing buffer (25 mM Tris, pH 7.5, 500 mM NaCl, 2 mM DTT, 5% glycerol) using an ATKA FPLC (GE Healthcare, Piscataway, NJ). Protein was eluted isocratically in sizing buffer over 1.3 column volumes at a flow rate of 2 ml/min collecting 3 mL fractions ...
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bioRxiv - Synthetic Biology 2021Quote: ... that had been pre-equilibrated with 1.2 column volumes of sizing buffer (25 mM Tris, pH 7.5, 250 mM NaCl, 2 mM DTT, 5% glycerol) using an ATKA Purifier (GE Healthcare, Piscataway, NJ). Protein was eluted isocratically in sizing buffer over 1.3 column volumes at a flow rate of 2 ml/min collecting 3-ml fractions ...
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bioRxiv - Biochemistry 2020Quote: ... 100 μL purified proteins at 2-5 mg/mL were injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in gel filtration buffer ...
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bioRxiv - Bioengineering 2020Quote: ... Angiography was also performed with a bolus intravenous injection of 2 ml/kg iohexol (Omnipaque™, 300 mg I/ml; GE Healthcare) using a CT-scanner (CT ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 2×106 CAFs were transfected with 3 nM of non-targeting control (D-001810-10-05, GE Healthcare Dharmacon, Inc., Lafayette, CO) or THY1 human siRNA (L-015337-00-0005 ...
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bioRxiv - Molecular Biology 2023Quote: ... 40 W for 2–2.5 h) followed by detection on an Amersham Typhoon Gel and Blot Imaging System (GE Healthcare Life Sciences).
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bioRxiv - Synthetic Biology 2024Quote: ... the pellet was resuspended in 18 mL of MCB1 with 0.1% BSA and 2-4.5 mL of material was layered onto stepwise Percoll (GE Healthcare, Chicago, IL, USA) MCB1 gradients with 0.1% BSA ...
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bioRxiv - Cell Biology 2024Quote: Human HeLa (ATCC® CCL-2™) were cultured in DMEM (PanEco) supplemented with 10% fetal bovine serum (FBS; HyClone/GE Healthcare) and penicillin/streptomycin ...
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bioRxiv - Molecular Biology 2020Quote: ... 20 µl IgG Sepharose 6 Fast Flow beads (GE Healthcare) were washed twice with 1 ml ice-cold PA-150 buffer [150 mM KCl ...
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bioRxiv - Bioengineering 2019Quote: ... 6% Ficoll PM-400 (17-0300-10, GE Healthcare, Sweden), 0.2% sarcosyl (L7414 ...
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bioRxiv - Microbiology 2019Quote: ... Superose 6 10/300 GL (GE Healthcare, Chicago, IL, USA) equilibrated with 10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2019Quote: ... 6 mL cesium trifluoroacetate solution (CsTFA, GE Healthcare, Buckinghamshire, UK) and gradient buffer solution ...
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bioRxiv - Biochemistry 2021Quote: ... and finished with SEC (Superose 6 Increase, GE Life Sciences). Recombinant PA28γ was expressed in Rosetta E ...
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bioRxiv - Biochemistry 2021Quote: ... size-exchange chromatography (SEC) (Superose 6 Increase, GE Life Sciences) was performed on pooled fractions with proteolytic activity after Resource Q ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were loaded in a SuperoseTM 6 Increase (GE Healthcare) column ...
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bioRxiv - Microbiology 2020Quote: ... Affinity chromatography using Ni Sepharose 6 Fast Flow (GE Healthcare Life Sciences ...
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bioRxiv - Biophysics 2022Quote: ... SUR1core was further purified using Superose 6 increase (GE Healthcare) column running with TBS supplemented with 0.006% GDN ...
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bioRxiv - Cell Biology 2022Quote: ... before subjecting it to SEC (Superose 6 column, GE healthcare). For labeling purified KASH5 used in TIRF experiments ...
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bioRxiv - Immunology 2020Quote: ... Size exclusion chromatography using Superose 6 Increase column (GE Healthcare) yielded final purified protein.
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bioRxiv - Molecular Biology 2020Quote: ... followed by Superose 6 10/300 GL column (GE Healthcare) to a final concentration of 180 μg/ml in PBS.
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bioRxiv - Immunology 2022Quote: ... and a Superose 6 10/300 GL column (GE Healthcare) in 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2022Quote: Purified ORF8N78Q was loaded onto a Superose 6 (GE Healthcare) gel filtration column and eluted in 20 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: A nickel column (Ni Sepharose 6 Fast Flow, GE Healthcare) was washed with deionized water at pH 7.0 ...
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bioRxiv - Cell Biology 2019Quote: ... Lysates were incubated with IgG sepharose 6 beads (GE Healthcare) in XB buffer (100 mM KCl ...
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bioRxiv - Biophysics 2021Quote: ... using a Superose 6 increase 10/300 column (GE Healthcare) and an ÄKTA system in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... BLM was further purified using a Superose 6 (GE Healthcare) in buffer D (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... and injected to Superose 6 10/300 column (GE Healthcare) previously equilibrated in size exclusion chromatography (SEC ...
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bioRxiv - Genetics 2022Quote: ... or IgG Sepharose® 6 Fast Flow Beads (GE Healthcare) was added ...
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bioRxiv - Biochemistry 2023Quote: ... using a Superose 6 increase 10/300 column (GE Healthcare) and an Agilent LC-1220 system in 20 mM Mes-Tris pH 6.5 ...
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bioRxiv - Biochemistry 2023Quote: ... using a Superose 6 increase 10/300 column (GE Healthcare) and an Agilent LC-1220 system in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... lysates in PBS with protease and phosphatase inhibitor cocktails were injected (500 µL per injection, corresponding to 1–3 mg total protein) onto a Superose® 6 10/300GL column (GE Life Sciences) equilibriated with PBS (pH 7.2 ...
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bioRxiv - Immunology 2020Quote: Purified CoV-S trimers were concentrated to 1 mg/mL and loaded onto a Superose 6 Increase 10/300 GL column (GE Healthcare Life Sciences) and passed through a Wyatt DAWN coupled to a Wyatt UT-rEX differential refractive index detector (Wyatt Technology) ...
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bioRxiv - Microbiology 2021Quote: ... The MxiN and Spa33 were combined with an excess of MxiN and the mixture was concentrated to a final volume of 2 mL for injection onto a Hi Load 26/600 Superdex 200 pg column (GE Healthcare Life Sciences). The protein absorbance at 280 nm was monitored to detect protein elution on an AKTA system (GE Healthcare Life Sciences) ...
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bioRxiv - Plant Biology 2019Quote: Samples each containing 2 μg Chl a of ΔpsbO-PSII-M and ΔpsbO-NRC were precipitated using the 2D Cleanup Kit (GE Healthcare, Chicago, IL) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... DNA was stained with 0.2 µg/ml ethidium bromide (EtBr) (Nacalai Tesque, 14631-94) and detected using a Typhoon FLA9000 gel imaging scanner (GE Healthcare, Chicago, Illinois). Gel images were processed using ImageJ 1.8.0 (NIH ...
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bioRxiv - Cell Biology 2021Quote: ... of SARS-CoV-2: the sequence encoding the N-terminally GST-tagged cytoplasmic domain of S were cloned into the vector pGEX6p2 (GE Healthcare Life Sciences). For GST-S (1255-1273 ...
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bioRxiv - Biochemistry 2021Quote: ... of L-enantiomeric peptides CDP1 and CDP7 binding to SARS-CoV-2 3CLpro was determined by Surface Plasmon Resonance Spectroscopy (SPR) using a Biacore 8K instrument (GE Healthcare, Uppsala, Sweden). The peptides were immobilised on two separate channels on a series S CM-5 sensor chip (Cytiva ...
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bioRxiv - Cell Biology 2021Quote: ... hMBs and C2C12 cells were differentiated in a differentiation induction medium (DIM) consisting of DMEM with 2% horse serum (HyClone; GE Healthcare, UT, USA) and PS (Nihashi et al. ...
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bioRxiv - Neuroscience 2023Quote: ... A 20-μl droplet of virus inoculum containing 8X106 plaque-forming unit (PFU) of PRV-Becker, resuspended in medium (Dulbecco modified Eagle medium, 2% fetal calf serum and antibiotics) (Hyclone, GE Healthcare life sciences), was applied onto the abraded area of the skin ...
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bioRxiv - Neuroscience 2022Quote: ... and soluble form of VAMP-2 lacking the transmembrane domain (VAMP-21-96) were purified using a Mono S column (GE Healthcare, Piscataway, NJ) as described previously26 ...
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bioRxiv - Immunology 2023Quote: ... Correctly conformed HLA.A2-peptide complexes were purified using Ni2+-NTA agarose chromatography (HisTrap excel, 2 × 5 ml2 columns connected in series; GE Healthcare Life Sciences) followed by size-exclusion chromatography (Superdex 200 10/300 GL ...
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bioRxiv - Biochemistry 2023Quote: The Exosc3 open reading frame encoding the mouse EXOSC3 protein was cloned into pGEX-6P-2 plasmid (GE Healthcare Life Sciences (now Cytiva)) to create an N-terminally glutathione-S-transferase (GST ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated to 2-3 mL for separation on exclusion size chromatography (HiLoad 16/600 SuperdexTM 75pg, GE Healthcare; ÄKTA Avant). Finally ...
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bioRxiv - Biophysics 2023Quote: ... size exclu-sion chromatography was conducted by injecting 2-5 mL sample onto HiLoad 16/600 Superdex 200 Column (GE Healthcare Life Sciences) connected to a BioRad NGC Quest 10 FPLC system ...