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Citations for GE Life Sciences :
1101 - 1150 of 3820 citations for 6 Pteridinepropanoic acid 2 4 diamino α 4 methoxycarbonyl phenyl α 2 propyn 1 yl methyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 N NaOH) and 2 h with transfer buffer (1.5 M NaCl, 0.25 N NaOH) using a VacuGene XL (GE Healthcare). The DNA was fixed to the membrane by soaking it in freshly prepared 0.4 N NaOH for 10 min ...
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bioRxiv - Biochemistry 2024Quote: ... 5 mM 2-mercaptoethanol and desalted into the same buffer using a 5 mL HiTrap desalting column (GE Healthcare) using an AKTA FPLC system (Cytiva ...
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bioRxiv - Microbiology 2023Quote: ... the buffer was exchanged with 1% acetic acid at pH 3 and 0.02% sodium azide by using a HiPrep 26/10 desalting column (GE Healthcare). This ensured that the proteins were maintained in their monomeric form ...
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bioRxiv - Molecular Biology 2023Quote: ... The signals of the transferred radioactively labelled methyl groups were detected by autoradiography using a Hyperfilm™ high performance autoradiography film (GE Healthcare) at −80 °C in the dark ...
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bioRxiv - Cell Biology 2019Quote: ... using a HiTrap NHS-Activated HP affinity column coupled with aIL-4 monoclonal antibodies and an ÄKTA Fast Protein Liquid Chromatography (FPLC) instrument (GE Healthcare, Piscataway, NJ, USA). Immunizations ...
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bioRxiv - Biochemistry 2020Quote: ... lysates were incubated overnight at 4°C with 400 μl of Glutathione Sepharose 4B per l of expression culture (GE Healthcare GE17-0756-01) with gentle rotation ...
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bioRxiv - Microbiology 2021Quote: ... Purified 6x-His-SUMO or 6x-His-SUMO-Pat1 were coupled to NHS-activated Sepharose 4 fast flow resin (GE Healthcare, 17-0906-01) in ligand coupling buffer (200 mM NaHCO3 ...
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bioRxiv - Cancer Biology 2020Quote: ... Western blots were visualized and quantified with enhanced chemiluminescence and imaging on a Fuji LAS 4 000 image station (GE Healthcare, Chicago, IL, US)4.
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bioRxiv - Neuroscience 2022Quote: ... and 500 μg of total protein from the supernatants was precleared with Protein G Sepharose 4 Fast Flow (GE Healthcare, catalog #GE17-0618-01) for 2 h at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... 20 µg of each sample was loaded onto a 4-20% Gel (Bio Rad, Hercules, CA) for electrophoresis and transferred to a PVDF membrane (GE Healthcare Amersham, Chicago, IL). For cryba4 (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... #S0282).[1] Reverse transcription was performed in 1.5 mL microfuge tubes under end-over-end rotation using a modified Reverse Transcription mix (1x Maxima H-RT buffer, 4% Ficoll PM-400 (GE Healthcare, #17-0300-05), 3 mM MgCl2 (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 4000 rpm at 4°C for 20 minutes in order to determine the total protein concentration through NanoVue spectrophotometer (GE Healthcare, Chicago, IL, USA). A total of 40 µg protein was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Biophysics 2020Quote: ... SEC was conducted at 4°C using a Superdex 200 5/150 column on an AKTA Pure System (GE Healthcare Life Sciences, Marlborough, MA) with a flow rate of 0.15 mL/min and 0.5 s exposures ...
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bioRxiv - Bioengineering 2020Quote: ... depending on machine availability and the type of imaging performed: LOGIQ Book XP with 8L-RS 4-12 MHz linear array probe (GE Healthcare, Milwaukee, WI, USA), Aixplorer with L14-5 probe (Supersonic Imagine ...
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bioRxiv - Molecular Biology 2023Quote: ... Concentrated media was then subjected to size exclusion chromatography using Sepharose 4 fast flow column connected to the ÄKTA prime system (both GE Healthcare, Chicago, IL, USA) and eluted in PBS with a flow rate of 0.5 ml/min ...
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bioRxiv - Biophysics 2021Quote: ... to 100% buffer B (6 M urea, 20 mM Tris pH 9.0, 1 M NaCl) of an AKTA FPLC system (GE Healthcare). Fractions containing full-length H1 were pooled and purified on a semi-preparative C18 HPLC column on a gradient of 0—70% buffer B and freeze-dried until use ...
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bioRxiv - Cell Biology 2020Quote: 80 μg of the purified recombinant DNC-1 fragments were loaded on a Superose 6 10/300 column (GE Healthcare) equilibrated with storage buffer (25 mM HEPES ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1 mM DTT.) Cascade was further purified by size exclusion chromatography using a Superose 6 increase column (GE healthcare) in SEC buffer (25 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: Protein samples within the concentration range 0.5-1 mg/mL were injected onto pre-equilibrated Superose 6 10/300 size-exclusion chromatography column (GE Healthcare) in buffer containing 20 mM Tris-Cl ...
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bioRxiv - Cell Biology 2020Quote: ... followed by purification via Superose 6 (GE Healthcare). The proteins were frozen in liquid nitrogen and stored at −80 °C.
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bioRxiv - Molecular Biology 2021Quote: ... IgG Sepharose 6 Fast Flow beads (GE Healthcare) were used ...
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bioRxiv - Immunology 2021Quote: ... or pH 6-11 (GE Healthcare, Uppsala, Sweden). With IPG strip reduction ...
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bioRxiv - Neuroscience 2019Quote: ... 6% Ficoll PM-400 (GE Healthcare/Fisher Scientific), 0.2% sarkosyl (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... or IgG Sepharose (6 Fast Flow, GE Healthcare) (500 μl per 500 ml culture) ...
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bioRxiv - Microbiology 2021Quote: ... Ni Sepharose 6 Fast flow resin (GE Healthcare) affinity exclusion chromatography was performed to obtain the tagless protein (containing the tag C-terminal sequence ...
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bioRxiv - Bioengineering 2023Quote: ... and AKTA flux 6 system (GE Healthcare, USA). The system and cassette were sanitized with 1.0 N NaOH before and after diafiltration ...
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bioRxiv - Biochemistry 2023Quote: ... or Superpose 6 10/300 GL (GE Healthcare) size exclusion column ...
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bioRxiv - Molecular Biology 2023Quote: ... 6-mm-diameter Whatman paper discs (GE Healthcare) were placed on top of the plate and wetted with 5 μl of a 10 mM TcmX solution in DMSO or 300 μg of erythromycin diluted in 10 μl of ethanol ...
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bioRxiv - Immunology 2023Quote: ... Superose 6 Increase 10/300 GL (GE Healthcare) in a mobile phase of PBS containing 300 parts per million of sodium azide and at a flow rate of 0.75 mL/min (Dionex Ultimate 3000 pump ...
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bioRxiv - Synthetic Biology 2024Quote: ... IMAC Sepharose 6 Fast Flow beads (GE Healthcare) was added into (Ribosome purification ...
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Molecular insights into the atypical activation mechanism of GPR156 in maintaining auditory functionbioRxiv - Molecular Biology 2024Quote: ... filter and subjected to Superose 6 (GE Healthcare) Increase column (GE Healthcare ...
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bioRxiv - Neuroscience 2019Quote: ... Each probe contains a 3’-end amine-modified nucleotide that allows directly couple to an NHS-ester Cy3 dye (GE Healthcare, PA23001) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... Unconjugated TFP ester dyes were separated from labeled proteins using HiTrap Desalting columns with Sephadex G-25 resin (GE Healthcare Life Sciences). Alexa Fluor™ 647 C2 Maleimide (A20347 ...
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bioRxiv - Biophysics 2019Quote: ... we use this amino linker to fluorescently tag the probes with a Cy3B NHS ester purchased from GE Healthcare (Mississauga, ON, Canada) and dissolved at a concentration of 1 mg/mL in dimethyl sulfoxide (DMSO) ...
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bioRxiv - Cell Biology 2022Quote: For whole mount staining of embryonic hearts Ad2 was directly labelled via an NHS ester by the fluorescent dye Cy2 according to the instructions of the manufacturer (GE Healthcare, #32000). Ad2-Cy2 was applied at a concentration of 6 μg/ml in PBS/0.1%Tx100/5% goat serum for 2 hr at room temperature to paraformaldehyde fixed E10.5 hearts followed by several washing steps ...
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bioRxiv - Molecular Biology 2019Quote: ... concentrated to a volume of 2 ml and applied onto a S75 16/600 pg size exclusion column (GE Healthcare), equilibrated with 10 mM HEPES pH 7.2 ...
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bioRxiv - Biochemistry 2020Quote: ... and 2 mM DTT for further purification by anion exchange on a Mono Q 5/50 GL column (GE Healthcare) with a gradient from 80 mM to 1 M NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... Cleaved protein was applied to a Source 15 Q anion exchange column and eluted with a gradient of 200 mM-300 mM NaCl in 20 mM HEPES (pH 7.0) and 2 mM DTT followed by size exclusion chromatography using a Superdex 200 prepgrade column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was collected and incubated with 2 column volume (for 20 mL supernatant) of Gluthatione Sepharose 4B (GE Healthcare) for 1h30 on a rotating wheel ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted protein sample was dialyzed against 20 mM Tris-HCl (pH 8.0) and 2 mM CaCl2 (buffer B) and applied to a Mono Q 10/100 GL column (GE Healthcare) equilibrated with buffer B ...
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bioRxiv - Biophysics 2021Quote: ... Proteins were expressed in Rosetta 2 bacteria and purified by affinity chromatography using a HiTrap™ chelating column (GE Healthcare) according to the manufacturer’s instructions in 50 mM Tris at pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... was expressed in E.Coli Rosetta 2 (DE3) and purified by affinity chromatography over a Sepharose 4B GSH affinity column (GE healthcare) followed by gel filtration over a Hiload Superdex 200 column (GE healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... Purified Ycf1p NBD1 and NBD1-R were exchanged into the fluorescence buffer (20 mM Na3PO4, 2% glycerol, 150 mM NaCl, pH 7.3) by size exclusion chromatography (Superdex75, GE Healthcare). MgATP-bound and apo NBD1 and NBD1-R were generated by adding 2 mM MgATP or 2 mM EDTA ...
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bioRxiv - Biochemistry 2020Quote: ... and the resulting supernatant was passed through either a 0.45 or 0.8 μm pore size filter and loaded onto a 5 mL Ni+2-NTA HisTrap HP column (GE Healthcare) pre-equilibrated with binding buffer (20 mM imidazole ...
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bioRxiv - Cell Biology 2021Quote: ... Cleaved protein was applied to a Source 15 Q anion exchange column and eluted with a gradient of 150 mMà350 mM NaCl in 20 mM Immidazole (pH 8.0) and 2 mM DTT followed by size exclusion chromatography using a Superdex 200 prepgrade column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Immunology 2021Quote: ... Nbs and their mixture (1.6 nmol of SARS-CoV-2 RBD mixed with 1.6 nmol of Nbs) were run over a Superdex 75 column (GE healthcare) at a flow rate of 0.5 mL/min with AKTA pure.
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bioRxiv - Cell Biology 2021Quote: ... Plasmids encoding for GST fusion proteins of Cobl-like1-411 were generated by subcloning into pGEX-4T-2 (GE Healthcare). A plasmid encoding for TrxHis-Cobl-like1-411 was generated by PCR and subcloning into pET-32 (Novagen ...
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bioRxiv - Immunology 2020Quote: The binding kinetics and affinity of mAbs to SARS-CoV-2 RBD were analyzed by SPR (Biacore T200, GE Healthcare). Specifically ...
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bioRxiv - Cell Biology 2022Quote: ... Time-lapse images (5-10 z-sections, 0.5–2 μm separation) were deconvolved using SoftWoRx software (Applied Precision, GE Healthcare) and processed with FIJI/ImageJ ...
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Bridging of nucleosome-proximal DNA double-strand breaks by PARP2 enhances its interaction with HPF1bioRxiv - Biochemistry 2019Quote: ... Clear lysates were filtered through 0.45 μm and immediately applied at 2 mL/min onto a HisTrap HP 5 mL column (GE Healthcare) previously equilibrated in lysis buffer ...