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Citations for GE Life Sciences :
1301 - 1350 of 3676 citations for 5 1 3 benzothiazol 2 ylamino pentanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: Purified protein or cell lysates were injected onto a Superose 6 increase 5/150 column (GE Healthcare), running in a buffer containing 20 mM Tris (pH 7.5) ...
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bioRxiv - Molecular Biology 2020Quote: ... The cell-free lysate was applied into a 5 ml Hi Trap Chelating HP column (GE Healthcare) equilibrated in start buffer ...
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bioRxiv - Biophysics 2020Quote: ... the solution was loaded on a 5 mL Ni-NTA HiTrap chelating column (GE Healthcare, Chicago IL) that was equilibrated in Ni-NTA base buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... The labeled viruses were purified from excess dyes on a Nap-5 gel filtration column (GE Healthcare) that was equilibrated with 50 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... the supernatant was loaded onto a HisTrap column (5 mL, cat. No. 17-5248-01, GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2020Quote: ... After cell disruption the cleared lysate was loaded on a 5 ml HisTrap FF column (GE Healthcare) equilibrated in lysis buffer A and the protein was eluted with a gradient of 25 – 250 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... followed in tandem by a HiTrap™ Q Sepharose HP column (5 ml/min, GE Life Sciences). The SP Sepharose column was removed before the protein was stepped off from the Q sepharose with Buffer B2 (20 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting protein fraction was further purified on a 5 ml Hi-Trap HP SP (GE Healthcare) cation exchange column using an Akta Pure FPLC ...
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bioRxiv - Molecular Biology 2020Quote: ... Rubisco-containing fractions were applied to an anion exchange column (MONO Q 5/50 GL, GE Healthcare) and eluted with a linear salt gradient from 30 to 500 mM NaCl in lysis buffer.
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bioRxiv - Microbiology 2020Quote: ... Purification was done using 5 mL pre-packed Ni-sepharose AKTA Hi-TRAP column (GE Healthcare, USA) equilibrated (100 mM NaH2PO4 ...
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bioRxiv - Cell Biology 2021Quote: ... The next morning the cleaved protein was loaded on a 5 ml HiTrap HeparinHP column (GE Healthcare). The protein was eluted over a gradient from 100 mM to 2M KCl ...
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bioRxiv - Biophysics 2021Quote: ... the solution was loaded onto a 5 mL Ni-NTA HiTrap chelating column (GE Healthcare, Chicago IL) that was equilibrated in Ni-NTA base buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... 100 mM NaCl pH 7.0 and further purified on a 5 ml Heparin affinity column (GE Healthcare) with a 100 ml gradient of 0.1 to 1.5 M NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... and 5 was further purified by gel filtration on a 16/600 Superdex 200 column (GE Healthcare) in S200 buffer ...
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bioRxiv - Biochemistry 2022Quote: ... or using an ÄKTA Prime purification system (Amersham Biosciences) equipped with 5 mL HisTrap column (GE Healthcare), with an imidazole gradient of buffer B (20 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was then loaded onto a 5 ml HiTrap SP FF column (GE Healthcare Life Sciences) followed by gradient elution with elution buffer SB (50 mM Tris pH 8 ...
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bioRxiv - Immunology 2022Quote: ... the sample was again loaded onto a HisTrap™ HP 5 ml column (GE Healthcare, Diegem, Belgium). The flow-through was collected and concentrated before injection onto a HiLoad® 16/600 Superdex® 75 pg column (GE Healthcare Diegem ...
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bioRxiv - Physiology 2022Quote: ... Leukocytes were separated from debris by resuspending the pellet in 5 ml 33% Percoll solution (GE Healthcare). Erythrocytes were lyzed using an ACK lysis buffer (155 mM NH4Cl ...
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bioRxiv - Cell Biology 2022Quote: ... then loaded onto a 5 mL Histrap column (Cytiva) connected to an FPLC AKTAxpress system (GE Healthcare). After affinity binding ...
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bioRxiv - Biochemistry 2022Quote: ... pH 8.0 and 5 mM β-mercaptoethanol) containing 100 mM NaCl by using desalting column (GE Healthcare) and further purified by affinity chromatography with HiTrap heparin column (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... and 0.5 mM EDTA) and loaded onto a 5 mL RESOURCE Q anion exchange chromatography (GE Healthcare). Hsp104 was eluted via linear gradient of buffer Q+ (20 mM Tris pH = 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... 5 mL of supernatant was collected and loaded to a Sephacryl S-300 HR column (GE healthcare) pre-balanced with G-buffer ...
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bioRxiv - Immunology 2022Quote: ... Filtrated culture supernatants were purified with a 5 ml HisTrap FF Crude column (GE Healthcare Life Sciences) previously equilibrated in Buffer A (20 mM NaH2PO4 ...
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bioRxiv - Neuroscience 2021Quote: ... Sample was concentrated to ∼5 mg/mL and injected onto a Superose 6 Increase column (GE Healthcare) equilibrated in SEC buffer ...
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bioRxiv - Biochemistry 2020Quote: ... the proteins were buffer exchanged into the appropriate buffer using an Illustra NAP-5 column (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... Periplasmic proteins were extracted and CusF was purified on a 5 ml StrepTrap HP column (GE healthcare) equilibrated with buffer A (10 mM NaPi ...
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bioRxiv - Immunology 2022Quote: ... 5% Glucose for further purification by cation-exchange chromatography using HiTrap SP High-Performance columns (GE Healthcare), using a gradient elution with 20mM HEPES pH 6.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... Survivin was purified by immobilized metal ion chromatography (IMAC) with a 5 ml HisTrap FF (GE Healthcare) column followed by overnight dialysis in 50 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... The cleaved receptor solution was then loaded onto a 5 mL HiTrap SP HP column (GE Healthcare) using an Akta Start system (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... Size exclusion chromatography was performed on Superdex™ 200 Increase 5/150 GL (GE Healthcare, Chicago, IL).
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bioRxiv - Molecular Biology 2021Quote: ... The lysates were cleared by centrifugation and loaded onto a 5 ml HisTrap HP column (GE Healthcare). After washing with buffer A (50 mM Tris pH 8.0 ...
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bioRxiv - Cancer Biology 2020Quote: ... Eluted protein was further purified using an anion exchange column (HiTrap Q HP 5 ml; GE Healthcare) before buffer exchange into PBS and storage at –20°C.
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bioRxiv - Biochemistry 2021Quote: ... Then the mixture was purified by Mono Q 5/50 GL ion-exchange chromatography (GE Healthcare, USA), resulting in a stable nsp7-nsp8-nsp12 complex ...
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bioRxiv - Biochemistry 2020Quote: ... and the cleaved sample was loaded onto a HisWash2 equilibrated 5 ml HisTrap HP column (GE Healthcare). The flow-through was collected as 2 ml fractions ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were diluted to 75 mM NaCl and loaded onto a MonoQ 5/50 GL (GE Healthcare) equilibrated in QO buffer (20 mM Tris pH 8 ...
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bioRxiv - Immunology 2021Quote: ... Supernatant was harvested after 4-5 days and antibody purified on Protein G Sepharose (Abcam, GE Healthcare) following standard protocols ...
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bioRxiv - Immunology 2020Quote: ... Microglia were separated from debris by resuspending the pellet in 5 ml 33% percoll solution (GE Healthcare). Mast cells and macrophages were obtained from the peritoneal cavity by lavage with 5 ml cold PBS + EDTA (2 mM ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Membranes were washed (6×5 min) with TBS-T and proteins were visualized by quimioluminiscence (GE Healthcare Amersham™ ECL™ Western Blotting Detection Reagents ...
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bioRxiv - Biochemistry 2021Quote: ... The soluble fraction was applied to a 5 mL HisTrap HP Ni2+-NTA IMAC column (GE Healthcare) pre-equilibrated with lysis buffer and washed with 50 mM imidazole ...
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bioRxiv - Biophysics 2020Quote: ... and the clarified cell extract was loaded onto a 5-mL HisTrap HP column (GE Life Sciences) equilibrated in HisTrap Buffer A ...
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bioRxiv - Microbiology 2021Quote: ... 100μg/ml of streptomycin and 5% fetal calf serum (FCS; HyClone, GE Healthcare, South Logan, UT, U.S.A.) at 37°C in a humidified atmosphere with 5% CO2.
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bioRxiv - Immunology 2020Quote: IgGs were purified from plasma samples using 5-mL HiTrap MabSelect SuRe columns (GE Healthcare Life Sciences). Heat-inactivated plasma was diluted 10-fold with cold PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... the HisTrap FF column was additionally coupled to a HiTrap Heparin HP 5-ml column (GE Healthcare). Both proteins were subjected to TEV protease cleavage at 4 °C overnight and another HisTrap FF 5-ml column purification step ...
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bioRxiv - Molecular Biology 2021Quote: ... Extract was prepared as above and was passed through a 5 mL HisTrap HP column (GE Healthcare). The column was washed with 10 CV of IMAC buffer ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... CirpA4 and CirpA5 were further purified by ion exchange chromatography (Mono Q 5/50 column, GE Healthcare). Homogeneous protein populations eluted in the peak (CirpA3 ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... protein extract was fractionated by anion exchange chromatography using a MonoQ 5/50 GL column (GE Healthcare), washed with 10 column volumes (CV ...
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bioRxiv - Immunology 2021Quote: ... affinity chromatography with in-line dialysis in PBS via HiTrap 5 ml desalting columns (GE Healthcare – 29048684) using an Akta™ Pure system (GE Healthcare) ...
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bioRxiv - Immunology 2021Quote: ... IFN-α2 protein was column-purified using Nap-5 columns (GE Healthcare, Chicago, IL; #17-0853-01), incubated with 2.5ul serum ...
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bioRxiv - Immunology 2020Quote: ... 5-10 μg denaturated proteins were resolved by SDS-PAGE and transferred to nitrocellulose membranes (GE Healthcare). Antibodies against HOIL1 (H-1 ...
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bioRxiv - Immunology 2020Quote: ... Culture supernatants were clarified by centrifugation and purified through a 5 mL HisTrap FF column (GE Healthcare), using theÄKTA Pure chromatography system (Cytiva) ...