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Citations for GE Life Sciences :
1251 - 1300 of 3676 citations for 5 1 3 benzothiazol 2 ylamino pentanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... 2 μg of patient-derived AABs were dotted on a nitrocellulose membrane (GE Healthcare), membranes were probed with mouse anti-human IgG1-POD ...
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bioRxiv - Plant Biology 2023Quote: ... The extracted protein solution was quantified using the 2-D Quant Kit (GE Healthcare) following the manufacturer’s instruction.
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bioRxiv - Immunology 2022Quote: ... for 10 min and blocked in PBS containing 2% bovine serum albumin (GE Healthcare) and 0.01% Tween 20 (National Diagnostics ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The samples were then treated with the 2-D Clean-Up Kit (GE Healthcare) to eliminate impurities not compatible with mass spectrometry analyses and recovered in 50 mM ammonium bicarbonate buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse anti-BrdU (1:1; GE Healthcare, RPN202); mouse anti-PCNA (1:500 ...
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bioRxiv - Microbiology 2020Quote: ... ELISA plates were washed 3 times with PBST and 50 µL of sheep anti-mouse IgG-horseradish peroxidase (HRP) conjugated antibody (GE Healthcare) was added at a dilution of 1:3,000 in blocking solution ...
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bioRxiv - Biophysics 2022Quote: ... the volume was reduced to a volume of 400 ml using the ÄktaFlux system with a 3 kDa MWCO cartridge (GE Healthcare). Afterwards the system was used for buffer exchange with 50 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... 5’-FAM-UGUUCUUCUGGAAGUCCAGTT-3’) was separated by gel electrophoresis (20% polyacrylamide gel containing 7 M Urea) and visualized with a Typhoon FLA9500 (GE Healthcare).
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bioRxiv - Molecular Biology 2021Quote: ... The reaction took place for 3 h and the radio labeled kCR probe purified in a Sephadex G25 column (Amersham, GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... Purified 6-His-PI3Kγ catalytic subunit17,18 was used as the soluble analyte and was injected at different concentrations over the immobilized GRK2 in SPR buffer (0.01 M HEPES pH 7.4, 0.15 M NaCl, 3 mM EDTA, 0.005% surfactant P-20, GE Healthcare Life Sciences, Biacore Inc.). Binding experiments were performed at 25°C in SPR buffer with a flow rate of 20 μl/min ...
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bioRxiv - Biophysics 2021Quote: FH1FH2 constructs of Cdc12p (residues 882-1375) and Bni1p (residues 1227-1776) were expressed from pGEX-4T-3 plasmids (GE Healthcare) in BL21(DE3 ...
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bioRxiv - Cell Biology 2020Quote: ... N571 and N442 in frame with N-terminal tag glutathione S-transferase (GST) in pGEX-6P-3 expression plasmids (GE healthcare) were expressed in BL21-CodonPlus-RP E ...
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bioRxiv - Biochemistry 2022Quote: ... The fractions containing the protein of interest were pooled and concentrated to ∼3 mg/mL using a 100kDa MWCO Vivaspin protein concentrator (GE Healthcare). The concentrated protein was subjected to size exclusion chromatography on Superose 6 increase 10/300 column (GE Healthcare).
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bioRxiv - Immunology 2022Quote: ... ELISA plates were afterwards washed 3 times with PBST and 50 μL of anti-mouse IgG-horseradish peroxidase (HRP) conjugated antibody (Cytiva, GE Healthcare) was added at a dilution of 1:3,000 in blocking solution ...
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bioRxiv - Biochemistry 2021Quote: ... Active HAP fractions eluting from 0.2–0.3 M KPi were pooled and separated on a Superose 6 XK 16/70 gel filtration column (130 ml, GE Healthcare) equilibrated with buffer D + 0.1 mM EDTA at 0.15 M KCl ...
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bioRxiv - Neuroscience 2022Quote: ... apposed them to a BAS-SR2040 phosphor screen (Fujifilm) for 3 days and imaged the slides using a phosphorimager (Typhoon FLA 7000; GE Healthcare). We drew ROIs onto the sections with standards using Multigauge software (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... The samples were then applied to IPG strips and separated in the first dimension with the IPGphor 3 system from GE Healthcare according to their isoelectric focusing (20 C ...
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bioRxiv - Cell Biology 2020Quote: Computerized axial tomography scanning (CT scan) was performed on anesthetized P30 mice [3% of Isoflurane (Isoba Vet)] using an eXplore ista PET-CTscan (GE Healthcare) with the following parameters ...
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bioRxiv - Cell Biology 2020Quote: ... antigen was CENP-AHCP-3(105-183)::6xHis) and detected using an HRP-conjugated secondary antibody (rabbit or mouse; GE Healthcare). For antibody production CENP-AHCP-3(105-183)::6xHis was expressed in E ...
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bioRxiv - Biochemistry 2021Quote: ... with the addition of 1 mg of 3C protease per 3 L of culture volume and further separated on a Superdex 200 HiLoad column (GE Healthcare) in 50mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: ... 50 mM NaCl) at approximately 3 mg/mL and then further purified by size-exclusion chromatography using a Superdex-200 column (GE Healthcare) equilibrated in solubilization buffer ...
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bioRxiv - Cell Biology 2020Quote: ... A probe was generated from a PCR fragment homologous to the 3’ sequence just outside of the targeted region using the AlkPhos direct labelling kit (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... injection of 185 MBq [123I]-2ß-carbometoxy-3ß-[4-iodophenyl]-N-[3-fluoropropyl]nortropane ([123I]FP-CIT, GE Healthcare, Amersham, UK). The SPECT data acquisition was begun after exactly 3 hours ...
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bioRxiv - Microbiology 2020Quote: ... the pirA gene was cloned into XhoI and EcoRI sites of pGEX-4T-3 vector (GE Healthcare Life Sciences, Freiburg, Germany), encoding recombinant PirA with N-terminal-fused GST-tag ...
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bioRxiv - Microbiology 2023Quote: ... The eluted fractions were concentrated to 3 ml and applied to 120 ml HiLoad 16/60 Superdex 200 size-exclusion column (GE Healthcare) equilibrated with 10 mM Tris-HCl ...
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bioRxiv - Biophysics 2024Quote: ... His6-GB1-tag was subsequently cleaved by TEV protease (3 mM) and separated from bZIP on a cation exchange column (HiTrap SP; GE Healthcare) using a 0–2 M gradient of sodium chloride ...
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bioRxiv - Microbiology 2024Quote: ... The eluted Cas1-Cas2/3 complex was concentrated and further purified by gel filtration chromatography (Superdex200 10/300 GL, GE Healthcare) in buffer C ...
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bioRxiv - Biochemistry 2022Quote: ... The mixture was incubated for 3 h at room temperature and loaded onto a Superdex 200 10/300 GL (GE Healthcare) sizeexclusion chromatography column using buffer B as mobile phase ...
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bioRxiv - Genomics 2022Quote: ... Samples were then loaded onto a 1% agarose gel containing 3% formaldehyde and 1x MOPS and run at 75 V in 1x MOPS for ~3 hs after which RNA was transferred onto a Hybond N+ membrane (GE Healthcare) overnight ...
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bioRxiv - Synthetic Biology 2022Quote: ... then wrapped into a clear foil and exposed in the cassette with the phosphor screen for 3 days and visualized on a Phosphorimager (Typhoon FLA 7000, GE Healthcare). prDC1416 was used to generate the blot in Fig ...
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bioRxiv - Cell Biology 2023Quote: ... control HUVEC or HUVEC 48 hr post-siRNA treatment were incubated with cytodex 3 microcarrier beads (17048501, GE Healthcare Life Sciences) overnight then resuspended in 2.2 mg/ml fibrinogen (820224 ...
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bioRxiv - Cell Biology 2024Quote: ... Parasites were synchronized to a 3-h age window by isolating late schizonts from a 60% Percoll (GE Healthcare, Life Sciences) gradient and culturing these for 3 h with fresh erythrocytes (104) ...
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bioRxiv - Developmental Biology 2024Quote: ... a cDNA fragment encoding the C-terminal portion of mouse Ccrk (residues 289-346) was amplified by PCR and subcloned into the pGEX-4T-3 vector (GE Healthcare). The GST-fused Ccrk protein was expressed in Escherichia coli strain BL21 (DE3 ...
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bioRxiv - Neuroscience 2024Quote: All scans were conducted in a 3 Tesla General Electric scanner with an eight-channel head coil (GE MR750; GE Healthcare). Anatomical images were acquired via T1-weighted ...
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bioRxiv - Cell Biology 2024Quote: ... cleared extracts were alkylated twice with 20 mM iodoacetamide (3 h at RT in the dark) and then cleaned by two passages on PD-SpinTrap G-25 columns (GE Healthcare). Samples were then clicked as explained before ...
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bioRxiv - Cell Biology 2020Quote: ... Gels were UV irradiated for 5 minutes and imaged using an Amersham Imager 600 (GE Life Sciences). Gels were then equilibrated in Tris-glycine transfer buffer for 5 minutes and transferred onto nitrocellulose membranes ...
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bioRxiv - Cell Biology 2020Quote: ... 5 mM β-Mercaptoethanol) with 3C protease and then loaded onto a HisTrap HP column (GE Healthcare). The protein was then concentrated and loaded on a Superdex 200 Increase 10/300 GL (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... The first purification step was affinity chromatography with a 5 mL HisTrap™ FF column (GE Healthcare) precharged with Ni2+ and equilibrated in buffer A (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... elution from the HisPur™ Cobalt Resin was applied to a 5 ml streptactin column (GE Healthcare) and washed with HBS ...
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bioRxiv - Biochemistry 2020Quote: ... The filtered sample was applied to a 5 mL HisTrap HP column (GE Healthcare, #17-5248-02), pre-equilibrated with Buffer N and 40 mM imidazole using a peristaltic pump ...
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bioRxiv - Biochemistry 2021Quote: ... The target was captured from the cleared lysate on a 5 ml HisTrap FF column (GE Healthcare) and eluted with a linear gradient from 10 to 250 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... followed by hydrophobic interaction chromatography (HIC) using a 5 mL HiTrap Phenyl FF (HS) column (GE Healthcare). For the ion exchange procedure ...
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bioRxiv - Biochemistry 2021Quote: AsChi18A was purified by immobilized metal affinity chromatography using a HisTrap FF 5 mL column (GE Healthcare). The periplasmic extract containing AsChi18A was applied on the column using a binding buffer consisting of 20 mM Tris-HCl pH 8.0 and 5 mM imidazole ...
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bioRxiv - Genomics 2021Quote: ... The diluent was reloaded on AKTA with 5 mL Hitrap Q HP column (GE Healthcare Life Sciences), followed by a wash with 35 mL buffer containing 20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... combined and further purified by anion-exchange chromatography using a MonoQ 5/50 GL column (GE Healthcare, buffer A ...
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bioRxiv - Biochemistry 2020Quote: ... The FdR9-containing flow-through was loaded on a 5 mL HiTrap Q HP column (GE Healthcare) and elution was done using a linear NaCl gradient (0.04 - 1 M ...
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bioRxiv - Biophysics 2020Quote: ... the mixture of tetramers was purified by anion exchange chromatography (MonoQ 5/50 GE Healthcare Life Sciences) using a column gradient from 0.1 to 0.4 M NaCl ...
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bioRxiv - Bioengineering 2020Quote: ... The 0.2 μm filtered cleaved product was injected in a 5 mL HiTrap SP HP (GE Healthcare) cationic exchange column and eluted with a gradient to buffer F (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was loaded into a 5 mL Ni–NTA affinity chromatography column (GE Healthcare, United States) that was pre-equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2021Quote: ... 300 mM NaCl (IMAC B buffer) and loaded onto a 5 mL HiTrap IMAC column (GE Healthcare) using an Äkta Start Chromatography system ...