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Citations for GE Life Sciences :
1201 - 1250 of 1348 citations for IL 23 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The final purification step was size-exclusion chromatography on a HiLoad 26/600 Superdex 75/200 pg column (GE Healthcare, Chicago, IL, USA) in a buffer containing 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Immunology 2023Quote: ... The EROS-NOX2-p22phox-7G5 Fab complex was concentrated and applied onto a Superose 6 Increase 10/300 GL column (GE HealthCare, Chicago, IL) in Buffer D (20 mM Tris pH 8.0 ...
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bioRxiv - Bioengineering 2024Quote: ... 2 μg DNA loaded per lane was separated by electrophoresis on 1% agarose gels and transferred to Hybond-N membranes (GE Healthcare, Chicago, IL) using capillary flow transfer method ...
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bioRxiv - Biochemistry 2024Quote: ... protein was loaded onto a pre-equilibrated (50 mM Tris-HCl 7.5 at 4 °C and 100 mM sodium chloride) Superdex 16/600 size exclusion column (GE Life Sciences, Chicago, IL) following Ni-NTA affinity chromatography to remove any protein aggregate ...
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bioRxiv - Cancer Biology 2024Quote: ... Twenty micrograms of total protein were loaded either on a 12% polyacrylamide-SDS gel and transferred to a 0.2 µm nitrocellulose membrane (No10600001, GE Healthcare; Chicago, IL, USA). Membranes were blocked with 5% bovine serum albumin (BSA ...
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bioRxiv - Immunology 2024Quote: ... 1:3 blood dilutions were made with sterile Roswell Park Memorial Institute (RPMI) 1640 medium (HyClone; GE Healthcare Life Sciences, Chicago, IL, USA) supplemented with 100 unit/mL penicillin and 100 mg/mL streptomycin (Gibco Life Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... cDNA clones of human Sec22b and Syntaxin2 and Syntaxin3 were obtained from Dharmacon (GE Healthcare) (Sec22b ...
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bioRxiv - Cell Biology 2020Quote: ... a set of ON-TARGET human siRNAs against Arp3 (J-012077-08, Dharmacon, GE Healthcare), Myh9 (J-007668-07 ...
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bioRxiv - Biochemistry 2020Quote: ... The cDNA of human gelsolin (UniProt ID: P06396) was purchased from Open Biosystems (GE Healthcare). The fragment encoding subdomains 4-6 (residues 434-782 ...
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bioRxiv - Immunology 2019Quote: Healthy human monocytes were isolated from whole blood by Ficoll-Paque PLUS (17144003, GE Healthcare) density centrifugation followed by magnetic selection with CD14 Human MicroBeads (130-050-201 ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: Human peripheral blood neutrophils were obtained from consenting healthy volunteers using discontinuous Percoll (GE Healthcare) density gradient centrifugation ...
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Metabolomics reveals nucleoside analogs for regulating mucosal-associated invariant T cell responsesbioRxiv - Immunology 2023Quote: ... We isolated human peripheral blood mononuclear cells (PBMCs) using the Ficoll-paque gradient (GE Healthcare) and removed the adhered monocytes after induced plastic adherence with cell culture flasks ...
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bioRxiv - Biophysics 2023Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll-Paque (GE Healthcare Life Science) from buffy coats donated by healthy ...
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bioRxiv - Microbiology 2019Quote: Isothermal titration calorimetry (ITC) experiments were performed at 20°C following standard procedures using a Microcal VP-ITC calorimeter (GE Healthcare, Chicago, IL, US). The E ...
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bioRxiv - Developmental Biology 2020Quote: ... The membranes were washed with Tris buffered saline containing Tween 20 (AppliChem; TBST) and incubated with secondary anti-rabbit (GE Healthcare; Chicago, IL, US) or anti-mouse (GE Healthcare ...
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bioRxiv - Physiology 2022Quote: ... 75 V) and gel image was taken using an Image Quant LAS 500 chemo luminescence CCD camera (GE healthcare, life sciences, Chicago, IL, USA).
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bioRxiv - Molecular Biology 2020Quote: ... 250 μl were applied to an Äkta pure chromatography system equipped with a Superdex 200 Increase 10/300 GL column (GE Healthcare, Chicago, IL, USA) and run at 6 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... Staining was detected using an HRP-conjugated anti-rabbit secondary antibody and Chemi-Lumi One L assay kit (Nacalai) on a ImageQuant LAS 4000 imager (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Microbiology 2019Quote: ... 75-90 μl of affinity-purified protein sample was loaded onto a Superdex 200 Increase 10/300 GL SEC column (GE Healthcare, Chicago, IL, USA) previously equilibrated with 20 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2020Quote: ... The recombinant protein was purified from the cleared lysate using a combination of metal affinity chromatography using nickel-affinity (GE Healthcare, Chicago, IL, USA) and size exclusion chromatography using 16/600 Superdex 75 prep grade columns (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: Fusion proteins of glutathione-S-transferase and mouse Clstn3 (JK091; aa 21–244) were produced in BL21 Escherichia coli and purified using a glutathione-Sepharose column (GE Healthcare, Chicago, IL, USA). Clstn1 peptides (JK025 ...
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bioRxiv - Neuroscience 2020Quote: ... in which they were precleared for 1 h using 30 μl (dry volume) of a 1:1 mixture of protein-A and protein-G-agarose beads (GE Healthcare, Chicago, IL, USA). Thereafter ...
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bioRxiv - Biochemistry 2020Quote: ... The clear upper phase of the lysate was desalted by size exclusion chromatography using a PD MiniTrap G-25 prepacked column (GE Healthcare, Chicago, IL, USA) with assay buffer as eluent ...
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bioRxiv - Bioengineering 2020Quote: Binding affinity characterizations of transcribed RNA biosensors were performed using a SPR assay on the Biacore X100 instrument from GE Healthcare (Chicago, IL, USA) as previously described8 ...
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bioRxiv - Biophysics 2021Quote: ... The SΔTM protein was then further purified by size exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare, Chicago, IL, USA) (Fig ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies were HRP-conjugated and bands were visualized by the ECL Western Blotting Detection Reagent (GE Healthcare, IL, USA Catalog number: 95038–560) and bands were quantified with Gel Doc system (Bio Rad ...
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bioRxiv - Immunology 2021Quote: ... and the homogeneity and the size of the antibodies were analyzed by using an analytical Superdex 200 10/300 column (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Cancer Biology 2020Quote: ... Western blots were visualized and quantified with enhanced chemiluminescence and imaging on a Fuji LAS 4 000 image station (GE Healthcare, Chicago, IL, US)4.
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bioRxiv - Biophysics 2022Quote: ... were purified by immobilized metal affinity chromatography (IMAC) using a HisTrap HP 5 mL column mounted on an Äkta Purifier System (GE Healthcare, Chicago, IL, USA). Fractions containing the His6-Sumo-peptide fusion in elution buffer (20 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... The sample was then injected in an Äkta Purifier System equipped with a Superdex Peptide 10/300 GL column (GE Healthcare, Chicago, IL, USA) equilibrated with ultrapure water ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction was performed at 37 °C for 16 h and terminated by desalting it to 50 mM ABC buffer with HiTrap Desalting column (GE Healthcare: Chicago, IL, USA). It was further purified using Ni-NTA resin and collected the flow-through to remove the side product ...
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bioRxiv - Microbiology 2020Quote: ... the samples were resuspended in mobile phase A and filtered through a 0.45 μm nylon membrane syringe filter (GE Healthcare, Chicago, IL, United States). Mobile phase A consisted of 0.15 M triethylammonium acetate (TEAA ...
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bioRxiv - Immunology 2020Quote: Blood samples were obtained from four healthy donors with approval of the local Ethics Committee and PBMC were isolated via density gradient centrifugation with Ficoll Paque Plus (GE Healthcare, Chicago, IL, USA). Monocytes were enriched by negative selection using a pan monocyte isolation kit (MiltenyiBiotec ...
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bioRxiv - Microbiology 2020Quote: ... the cDNA obtained in the section “Realtime and Endpoint RT-PCR detection of deltaviruses from animal specimens” was amplified using illustra GenomiPhi V2 Kit (GE healthcare; Chicago, IL, USA). The amplified DNA was then purified with innuPREP PCRpure Kit (Analytik Jena ...
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bioRxiv - Neuroscience 2021Quote: Whole-brain images will be acquired at the Lane Neuroimaging Laboratory at the University of Wisconsin–Madison HealthEmotions Research Institute (Department of Psychiatry) using a 3 Tesla GE MR scanner (Discovery MR750; GE Healthcare, Chicago, IL, USA). A high-resolution T1 image will be acquired with a fast spoiled gradient recalled echo sequence (8.2 ms TR ...
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bioRxiv - Developmental Biology 2022Quote: ... 20 µg of each sample was loaded onto a 4-20% Gel (Bio Rad, Hercules, CA) for electrophoresis and transferred to a PVDF membrane (GE Healthcare Amersham, Chicago, IL). For cryba4 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... The blots were developed using Radiance Plus chemiluminescent substrate (Azure Biosystems, Dublin, CA, USA) and the signals quantified using the ImageQuant LAS-4000 mini Imager (GE Healthcare, Chicago, IL, USA). Primary hepatocytes were used as control.
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bioRxiv - Developmental Biology 2022Quote: Unbiotinylated ectodomain transfections were collected and quantified by ELISA using nickel-coated microplates and pooled for purification using nickel-sepharose columns (HisTrap HP, GE Healthcare, Chicago, IL, USA) and an AKTAxpress chromatography system (GE Healthcare) ...
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bioRxiv - Immunology 2022Quote: ... the target dsDNA from the PCR amplification was first concentrated by ethanol precipitation and then purified on an Akta Pure 150 FPLC instrument (GE Healthcare, Chicago, IL, USA) with a GE HiPrep 26/60 Sephacryl S-500 High Resolution size exclusion column and 0.3M NaCl running buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein were separated on 4-15 % Tris-glycine (TG) gels (#45101080003-2, SMOBIO, Hsinchu, Taiwan) and electro-transferred onto polyvinylidene fluoride (PVDF) membranes (#A16646282, GE Healthcare, Chicago, IL, USA) in TG buffer ...
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bioRxiv - Physiology 2019Quote: The optical density of cell suspensions was measured at a wavelength of 600 nm using an Ultrospec 10 spectrophotometer (GE Healthcare, Chicago, IL, USA). The cell dry weight (CDW ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were transferred to nitrocellulose membranes and visualized by enhanced chemiluminescence using ECL Prime Western Blotting Detection Reagent (#RPN2232, GE Healthcare; Chicago, IL, USA) and imager ImageQuant LAS 4000 (GE Healthcare ...
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bioRxiv - Cancer Biology 2019Quote: ... for 1 h incubated with the appropriate antibodies and then visualized using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Physiology 2021Quote: ... Membranes were again washed with 4 x 15 min TBS-T washes and a final 15 min TBS wash prior to band development with an ECL Prime Western Blot Detection Kit (GE Healthcare, Chicago, IL, USA) and imaged using a Chemidoc Imaging system (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... the samples were separated on an SDS-15% polyacrylamide gel and then electrophoretically transferred onto the nitrocellulose membrane Amersham Protran 0.45 µm NC (GE Healthcare Lifesciences, Chicago, IL, USA). Membrane-immobilized proteins were probed with mouse anti-linker histone H1 monoclonal antibody clone AE-4 (Upstate Biotechnology ...
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bioRxiv - Genomics 2021Quote: ... as follows: The proteinase K-treated reaction was combined with 1 μL of Sera-Mag carboxylate-modified magnetic particles (GE Healthcare, Chicago, IL, USA.) and 72 μL of binding buffer (300 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 4000 rpm at 4°C for 20 minutes in order to determine the total protein concentration through NanoVue spectrophotometer (GE Healthcare, Chicago, IL, USA). A total of 40 µg protein was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cellular lysates were cleared by centrifugation and 300 μg of total protein were added to 30 μL of pre-equilibrated streptavidin-sepharose matrix (GE Healthcare, Chicago, IL, USA). Following 1 h at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... and the size and purity of each antibody were determined by size exclusion chromatography using a Superdex 200 10/300 column (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Immunology 2021Quote: ... The blots were washed in TBS-T and incubated in secondary antibody in antibody buffer for 2 hours before washing again in TBS-T before visualising with ECL (GE Healthcare, Chicago, IL, USA) and imaging in an ALLIANCE 6.7 Chemiluminescence Imaging System (UVITEC ...