Labshake search
Citations for GE Life Sciences :
1151 - 1200 of 1348 citations for IL 23 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2022Quote: ... The eluate was concentrated and then applied to an initial size-exclusion chromatography (SEC) step using HiLoad 16/60 Superdex 200 (GE Healthcare, Chicago IL) in SEC200 buffer (50 mM Tris-HCl ...
-
bioRxiv - Plant Biology 2020Quote: Protein extracts (150 mg per sample) were incubated with 300 µl of IgG-Sepharose 6 Fast Flow beads (GE Healthcare, Chicago, IL, USA) for 2 h at 4°C with gentle rotation (1000 rpm) ...
-
bioRxiv - Physiology 2020Quote: Distinct bands of correct size were cut from 1.5% agarose gel and isolated using illustra GFX PCR and Gel Band Purification Kit (GE Healthcare, Chicago, IL, USA), following manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2019Quote: ... centrifuged at 17,500 RPM for 60 minutes and the clarified lysate loaded onto a 5 mL HisTrap HP (GE Healthcare, Chicago, IL, USA). The HisTrap HP column was washed with wash buffer (25 mM HEPES (pH 7.5) ...
-
bioRxiv - Plant Biology 2019Quote: ... centrifuged at 17,500 RPM for 60 minutes and the clarified lysate loaded onto a 5 mL GSTrap HP (GE Healthcare, Chicago, IL, USA). The GSTrap HP column was washed with wash buffer (25 mM Tris (pH 8.0) ...
-
bioRxiv - Plant Biology 2019Quote: Samples each containing 2 μg Chl a of ΔpsbO-PSII-M and ΔpsbO-NRC were precipitated using the 2D Cleanup Kit (GE Healthcare, Chicago, IL) according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2020Quote: ... containing 10 mM Tris pH 8.0 and 20 mM NaCl and purified using a SEC HiLoad 26/600 Superdex 75 column (GE Healthcare, Chicago, IL, USA) in 25 mM sodium phosphate pH 6.4 with 100 mM NaCl for wt-PD-1 and PD-1 mutants or in PBS pH 7.4 for PD-L1 and PD-L1-Long.
-
bioRxiv - Biochemistry 2021Quote: ... The protein was concentrated to 2 mL in a 30 kDa MWCO spin concentrator and applied onto a HiLoad 16/600 Superdex 200 pg size exclusion chromatography (SEC) column (GE healthcare, IL, USA) equilibrated in 20 mM Tris-HCl pH 7.4 ...
-
bioRxiv - Biochemistry 2021Quote: ... The self-assembled crude octamers were purified by size-exclusion chromatography on a Superdex-200 10/300 GL column attached to an AKTA FPLC (GE Healthcare, Chicago, IL). Pure fractions were identified by 15% SDS-PAGE after coomassie staining ...
-
bioRxiv - Biochemistry 2020Quote: ... a single 0.5 ml aliquot of the final protein was thawed and analyzed by analytical size-exclusion chromatography using a 10/300 Superdex200 analytical column (GE Healthcare, Chicago, IL), with a flow rate of 0.5 ml/min.
-
bioRxiv - Biochemistry 2020Quote: ... TFF-treated culture supernatant was adjusted to 25 mM imidazole and applied to a 5 ml Ni Sepharose High Performance nickel-charged column (GE Healthcare, Chicago, IL) previously equilibrated in Buffer A + 25 mM imidazole ...
-
bioRxiv - Biochemistry 2021Quote: ... PCR reactions were analyzed by agarose gel electrophoresis with ethidium bromide staining and purified using the Illustra GFX PCR DNA and Gel Band Purification kit (GE Healthcare, Chicago, IL). Reactions (10 μL ...
-
bioRxiv - Microbiology 2020Quote: ... through three in-line HEPA vent filters (0.3-µm glass-fiber to 0.2-µm PTFE to 0.1-µm PTFE; Whatman, GE Healthcare Life Sciences, Chicago, IL, USA) and stored at 4°C until use ...
-
bioRxiv - Microbiology 2020Quote: ... Membranes were washed using 0.1% PBSt and secondary antibody incubated 1h at room-temperature using Dako Polyconal Goat Anti-Rabbit or Anti-Mouse Immunoglobulins/HRP (Aglient Technologies, Santa Clara, CA, USA) washed using 0.1% PBSt and visualized using ECL or ECL Select (GE Healthcare, Chicago, IL, USA) on ChemiDoc XRS+ System (Bio-Rad Laboratories ...
-
bioRxiv - Biophysics 2020Quote: ... Protein separation was carried out by SDS-PAGE in 10% bis-acrylamide gels and transferred to nitrocellulose membranes (Amersham Protran; pore size, 0.45 μm; GE Healthcare, Chicago, IL, USA). The membranes were blocked with 5% fat free milk in Tris-buffered saline (TBS ...
-
bioRxiv - Bioengineering 2022Quote: T1-weighted axial images of the non-dominant arm were obtained using a 3T MRI scanner (Discovery MR750w, GE Healthcare, Chicago, IL, USA) and a receiver 16-channel flex coil ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... or medium alone for 30 min at room temperature and added to 30 μL of pre-equilibrated streptavidin-sepharose matrix (GE Healthcare, IL, USA). Following 1 h at 4 °C ...
-
bioRxiv - Biochemistry 2021Quote: ... Equal amounts of protein (10–50 μg) from the total extract were separated on 8–10% SDS–polyacrylamide gels and transferred onto nitrocellulose blotting membranes (GE Healthcare, Chicago, IL) at 12 V for 20 min in a semi-dry Trans-Blot Turbo system (Bio-Rad ...
-
bioRxiv - Cell Biology 2022Quote: ... and expressed proteins were purified from the lysis supernatant by Nickel affinity and gel filtration (Superdex 75 26/60, GE Healthcare, Chicago, IL) chromatography ...
-
bioRxiv - Synthetic Biology 2020Quote: ... for 1 minute in the dark and either exposed to an X-ray film or scanned in an Amersham Imager 600 (GE Healthcare, IL, USA). Protease accessibility assays were performed as follows ...
-
bioRxiv - Cancer Biology 2019Quote: ... the gel was visualized with the Typhoon FLA 9500 laser scanner (FAM filter, 50 µm pixel and 450 V unless otherwise noted, GE Healthcare, Chicago, IL).
-
bioRxiv - Neuroscience 2019Quote: ... each volunteer received a high-resolution structural 3D T1-weighted magnetic resonance scan acquired on a General Electric MR750 3T scanner (MR750; GE Healthcare, Chicago, IL).
-
bioRxiv - Biochemistry 2020Quote: ... TFF-treated culture supernatant was adjusted to 25 mM imidazole and applied to a 10 ml Ni Sepharose High Performance nickel-charged column (GE Healthcare, Chicago, IL) previously equilibrated in Buffer A + 25 mM imidazole ...
-
bioRxiv - Microbiology 2020Quote: ... and same protein quantities were separated on a 10% SDS-PAGE gel under reducing conditions and transferred to 0.45 μm PVDF blotting membrane (GE healthcare, Chicago, IL, USA). Total ERK1/2 and phospho-ERK1/2 were detected using p44/42 MAPK antibody (1:1000 in 5% BSA/TBS-T ...
-
bioRxiv - Microbiology 2021Quote: ... The entire ectodomain of IFX was used as the analyte which was first purified and resolved by size exclusion chromatography on a Superdex 200 Increase 10/300 column (GE Healthcare, Chicago, IL) in HBS-EP (10 mM HEPES ...
-
bioRxiv - Immunology 2020Quote: ... proteins were purified with Ni-NTA resin and purified with size exclusion chromatography on an AKTAPURE FPLC S200 increase column (GE Healthcare; Chicago, IL).
-
bioRxiv - Microbiology 2019Quote: ... The bacterial protein PA1217 was fused to a GST-tag by cloning it into a pGEX-6P-1 vector (GE Healthcare, Chicago, IL) using the BamHI and EcoRI restriction sites (Thermo Scientific) ...
-
bioRxiv - Microbiology 2019Quote: Slides for cell surface curvature enrichment analysis were imaged on a DeltaVision OMX V4 BLAZE 3D microscope (GE Healthcare Life Sciences, Chicago, IL) equipped with Photometrics Evolve 512 emCCD cameras and a UPlanApo 100x/1.42 oil objective with oil matched for the sample refractive index ...
-
Hypertension drives microbial translocation and shifts in the fecal microbiome of non-human primatesbioRxiv - Pathology 2021Quote: Echocardiography was performed on each of the vervet monkeys in study 1 using a GE Logiq S8 ultrasound unit (GE Healthcare, Chicago, IL) using an approach that has been previously described.20 Cardiac morphometric and functional phenotypes include ...
-
bioRxiv - Biochemistry 2021Quote: ... concentration to 1-2 mL in 10 kDa MWCO spin concentrators and applied to either a HiLoad Superdex 75 pg 16/600 or a Superdex 75 Increase 10/300 GL SEC column (GE Healthcare, IL, USA) equilibrated in 20 mM Tris-HCl pH 7.4 and 150 mM NaCl ...
-
bioRxiv - Microbiology 2019Quote: ... Eluted proteins were further purified via size exclusion chromatography using a HiLoad 16/60 Superdex 200 gel filtration column (GE Healthcare, Chicago, IL) using an AKTAExplorer 100 FPLC (GE Healthcare) ...
-
bioRxiv - Molecular Biology 2019Quote: Epithelial skin cells were collected from male and female volunteers using a Whatman® FTA® Sterile Omni Swab (GE Healthcare, Chicago, IL). Participants were asked to swab the sides of their nose and behind their ears for approximately thirty seconds using swabs in order to maximize skin cell yield ...
-
bioRxiv - Bioengineering 2022Quote: ... dialysed into a Tris-saline buffer (Tris 10 mM, NaCl 150 mM, pH 7.4) then affinity purified on a protein A sepharose column (GE Healthcare, Chicago, IL, USA). Bound antibody was eluted with 0.1 M Glycine pH 2.4 and neutralised with 1 M Tris pH 8.5 ...
-
bioRxiv - Immunology 2022Quote: ... mFcRn) were purified using a HisTrap Ni NTA column followed by size exclusion chromatography (Superdex 200 Increase column; both GE Healthcare, Chicago, IL) using 20 mM phosphate pH 8.0 ...
-
bioRxiv - Plant Biology 2022Quote: ... HA-MED14 prey proteins were incubated with the affinity-purified GST-PIF4 or GST-HMR bait proteins immobilized on glutathione Sepharose 4B beads (GE Healthcare, Chicago, IL) at 4 °C for 2 h ...
-
bioRxiv - Physiology 2022Quote: ... and ran this mixture through handcast Tris-glycine gels prior to blotting onto nitrocellulose membranes with 0.1 µm pore size (GE Healthcare, Chicago, IL, US). Subsequently ...
-
bioRxiv - Microbiology 2023Quote: ... The blots were incubated with γ32-P 5’-labelled probe at 42°C overnight and imaged using a phosphor screen on a Typhoon FLA 7000 Variable Mode Imager System (GE Healthcare, Chicago, IL). Probe sequences are listed in Table S2.
-
bioRxiv - Neuroscience 2022Quote: ... were purified from bacterial lysate by affinity chromatography on a GSTrap FF column (Cat. No. 71-5016-96 AM, GE Healthcare, Chicago, IL) followed by dialysis against multiple changes of phosphate buffered saline (PBS) ...
-
bioRxiv - Microbiology 2022Quote: ... after which chemiluminescence reaction was conducted using Chemi-Lumi One (Nacalai Tesque) and was subsequently viewed using Amersham Imager 680 (GE Healthcare, IL, USA).
-
bioRxiv - Immunology 2022Quote: ... Protein was concentrated in 10 kDa molecular weight cutoff centrifugal concentrators and purified by size exclusion chromatography on an AKTAPURE FPLC (GE Healthcare, Chicago IL), using an S200 column followed by a S75 column ...
-
bioRxiv - Immunology 2022Quote: ... and purified from pre-conditioned cell media supernatant using Ni-NTA resin and size exclusion chromatography with a S200 column on an AKTAPure FPLC (GE Healthcare; Chicago, IL).
-
bioRxiv - Microbiology 2023Quote: WB was performed on a semi-dry western blot device transferred to an Amersham Protran 0.45 mm nitrocellulose (NC) blotting membrane (GE Healthcare, Chicago, IL, USA) at 52 mA for 45 min ...
-
bioRxiv - Immunology 2023Quote: ... membranes were washed 3x in PBS-T and then incubated with appropriate secondary antibody conjugated with horseradish peroxidase (GE Healthcare, Chicago, IL, USA). Chemiluminescence was accomplished using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare ...
-
bioRxiv - Neuroscience 2023Quote: ... The adeno-associated virus (AAV) vectors were produced by helper-free triple transfection and purified using affinity chromatography (GE Healthcare, Chicago, IL, USA). For production of AAV2.1 vector ...
-
bioRxiv - Immunology 2023Quote: ... The culture medium containing the secreted LALA-B3B9 recombinant immunoglobulin G (rIgG) was purified using the protein A affinity column (GE Healthcare, Chicago, IL). The eluted fractions from purification were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine the purity of the purified antibody ...
-
bioRxiv - Plant Biology 2023Quote: ... and their fluorescence was recorded by direct scanning of the gel using an image analyser (Typhoon 9600, GE Healthcare Bio-Science, Chicago, IL) as described (Tasaki et al ...
-
bioRxiv - Molecular Biology 2023Quote: ... The gel was exposed to a phosphoimager screen overnight at -20°C and the phosphoimager screen was scanned using the Typhoon FLA 9000 phosphoimager (GE Healthcare, Chicago, IL). Experiments were performed in triplicate to establish reproducibility ...
-
bioRxiv - Immunology 2023Quote: Peripheral blood mononuclear cells (PBMC) were isolated from heparinized blood samples using standard Ficoll-Paque Plus gradient separation (GE Healthcare, Chicago, IL, USA). Cells were stored in 10% dimethyl sulfoxide in fetal bovine serum (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2023Quote: ... Plasma was separated from the entire blood by a density gradient centrifugation using the Ficoll-Paque™ PLUS (GE healthcare, Chicago, IL, USA), and plasma aliquots were stored at −80°C.
-
bioRxiv - Cancer Biology 2023Quote: ... Plasma was separated from the entire blood by a density gradient centrifugation using the Ficoll-Paque™ PLUS (GE healthcare, Chicago, IL, USA), and plasma aliquots were stored at −80°C.