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Citations for GE Life Sciences :
1201 - 1250 of 4827 citations for 7 Methyl 1 5 dioxo 1 2 3 5 tetrahydro indolizine 6 carbonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The cleaved dCas9 protein was further purified with a 5 ml SP Sepharose HiTrap column (GE Life Sciences) in 20 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatants containing the protein extracts were then applied onto a HisTrap FF 5 ml column (GE Healthcare) loaded with nickel ions using an ÄKTA Start liquid chromatography machine (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... the supernatant containing complexes of Panx and Sov was loaded onto 5 ml HisTrap Fastflow column (GE Healthcare). After extensive washing with Binding buffer ...
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bioRxiv - Microbiology 2020Quote: ... BHK-21 cells were grown at 37 °C with 5% CO2 in DMEM (HyClone, GE Healthcare life sciences) supplemented with 10% bovine serum and 1 U/mL penicillin/streptomycin ...
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bioRxiv - Cell Biology 2019Quote: ... Equal amount of 5-10µg proteins were resolved by SDS-PAGE and transferred to nitrocellulose membranes (GE Healthcare). Membranes were revealed using a chemiluminescent HRP substrate (Millipore ...
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bioRxiv - Molecular Biology 2019Quote: ... 10 μM ZnCl2 and 5 mM β-mercaptoethanol) for XPA and Superdex 200 10/300 increase (GE Healthcare) equilibrated in a different storage buffer (400 mM NaCl ...
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bioRxiv - Genomics 2019Quote: ... The diluted supernatants were applied to a 5 mL HiTrap heparin column (HiTrap™ Heparin HP, GE Healthcare) coupled to an ÄKTA purifier system (ÄKTA explorer ...
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bioRxiv - Biophysics 2019Quote: ... The mixture of tetramers was then purified by anion exchange chromatography (MonoQ 5/50 GE Healthcare Life Sciences) using a column gradient from 0.1 to 0.4 M NaCl ...
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bioRxiv - Molecular Biology 2019Quote: ... Purification was done using an AKTA Purifier FPLC system on a HisTrap FF 5 ml column (GE Healthcare). The column was washed and equilibrated with 10 volumes of LB prior to sample loading ...
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bioRxiv - Biophysics 2020Quote: ... The mixture of tetramers was then purified by anion exchange chromatography (MonoQ 5/50 GE Healthcare Life Sciences) using a column gradient from 0.1 to 0.4 M NaCl ...
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bioRxiv - Biophysics 2022Quote: ... Supernatants were filtered by 0.45 μm and loaded onto a 5 mL HiTrap MabSelect SuRe column (GE Healthcare) equilibrated with COP Wash buffer (20 mM Hepes pH 7.4 and 500 mM NaCl) ...
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bioRxiv - Biochemistry 2021Quote: ... 30 min) and the supernatant was loaded onto a 5 mL HisTrap HP Ni Sepharose column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was loaded on a Ni-IMAC-HisTrap™ FF 5 mL column (GE Healthcare, Chicago, IL) at 3.5 mL/min ...
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bioRxiv - Cell Biology 2021Quote: ... Gene-edited NIH3T3 cells were cultured at 37°C in 5% CO2 in DMEM (GE Healthcare, Chicago, IL) containing 10% FBS ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing the complex at reasonable purity were loaded onto a 5 ml HiTrap Heparin column (GE Healthcare), and the bound protein was eluted with a gradient from buffer A to buffer B (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... and then loaded on to a HiTrap™ SP Sepharose HP column (5 ml/min, GE Life Sciences) followed in tandem by a HiTrap™ Q Sepharose HP column (5 ml/min ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was then passed over a HisTrap™ FF Ni2+ column (5 ml/min, GE Life Sciences) and washed with Buffer B (20 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2019Quote: ... supplemented with 20 mM imidazole and loaded onto a 5 mL HisTrap HP Ni2+ affinity column (GE Healthcare). After washing with several column volumes (CV ...
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bioRxiv - Biophysics 2021Quote: ... and the final product was obtained by running the solution through an 5 mL Hi-Trap (GE Healthcare) desalting column (Figure S1).
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bioRxiv - Biophysics 2020Quote: ... the medium was loaded overnight onto a ~5 ml anti-CD9 AR40A746.2.3 affinity column (7.5 mg antibody/ml CNBr Activated Sepharose 4B beads; GE Healthcare) in line of an AKTA explorer (GE) ...
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bioRxiv - Bioengineering 2022Quote: ... Further purification was performed by ion-exchange chromatography using mono Q 5/50 GL (GE HealthCare, Uppsala, Sweden) for each fusion protein in 20 mM Tris-HCl (pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting supernatant was incubated for 4-5 h at 4 °C on glutathione sepharose 4B (GE Healthcare) (3 mL per 2 L expression) ...
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bioRxiv - Biochemistry 2022Quote: ... The clarified supernatant was then passed over a Ni-NTA His- Trap FF 5 ml column (GE Healthcare) which was pre-equilibrated with loading buffer composed of 25 mM HEPES pH 7.0 ...
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bioRxiv - Microbiology 2022Quote: Solubilized porins were loaded onto an anionic-exchange column (Hitrap HQ, 5 mL, GE Healthcare Life Science, France) and subjected to detergent exchange with 0.1 M MES buffer pH 6.5 complemented with 0.1% lauryldimethylamine-oxide (LDAO ...
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bioRxiv - Biophysics 2022Quote: ... The clarified supernatant was then passed over a Ni-NTA His-Trap FF 5 ml column (GE Healthcare) which was pre-equilibrated with loading buffer composed of 25 mM HEPES pH 7.0 ...
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bioRxiv - Microbiology 2022Quote: ... The top fractions after IMAC were pooled and desalted using a 5 ml HiTrap Desalting column (GE Healthcare) equilibrated with 25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was added to 5 mL of equilibrated Glutathione Sepharose 4B resin (GE Healthcare Bio-sciences AB). The beads were washed in a column 3 times with 50 mL of Buffer C (10 mM phosphate buffer pH 7.4 ...
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bioRxiv - Biophysics 2022Quote: ... the α-Syn solution was subjected to anion exchange column (AEC) (HiTrap Q Hp 5 ml, GE healthcare) followed by size exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was then applied onto a Hi Trap affinity (5 ml) (Ni2+) column (GE Healthcare Life Sciences) The column was washed with 10 column volumes of buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... Dried membranes were scanned using maximum quality and resolution settings with an Amersham Typhoon 5 imager (GE Healthcare) outfitted with 785 nm and 685 nm lasers and IRlong 825BP30 and IRShort 720BP20 filters ...
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bioRxiv - Microbiology 2020Quote: ... Beads were isolated from the suspension by centrifugation (500 x g, 5 minutes) through Percoll Plus (GE Healthcare) in a 50 mL conical tube ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was filtered and loaded onto a 5 mL HisTrap HP column (GE Life Sciences, now Cytiva), followed by washing [0.05 M Tris pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... Cleaved DEPTOR protein was then collected and applied to a 5 mL HiTrap Heparin HP column (GE Healthcare) equilibrated with HEP-A buffer (50 mM HEPES pH 8 ...
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bioRxiv - Biochemistry 2021Quote: ... 4 °C) and the supernatant applied to a GSTrap 5 ml FF column affinity purification column (GE Healthcare) at 4 °C equilibrated with Tris-Cas-A buffer ...
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bioRxiv - Bioengineering 2019Quote: ... 5% CO2 in a humidified incubator in CMRL 1066 media (Mediatech) supplemented with 10% FBS (Hyclone, GE Healthcare), 20□mM HEPES buffer ...
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bioRxiv - Immunology 2022Quote: ... 5% glucose) and the proteins purified by affinity chromatography using a HiTrap Heparin High-Performance column (GE Healthcare) and gradient elution (0-100% NaCl ...
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bioRxiv - Biochemistry 2019Quote: ... His6–Ub-tagged SbtB7001 was purified from cell lysate using a 5 ml HisTrap FF column (GE Healthcare): the pre-equilibrated and lysate-loaded column was washed with binding buffer (20 mM NaH2PO4 ...
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bioRxiv - Biochemistry 2019Quote: ... Lysate was clarified by centrifugation and loaded onto a HisTrap™ High-Performance 5 mL columns (GE Healthcare) using a peristaltic pump ...
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bioRxiv - Molecular Biology 2019Quote: ... The proteins from the cleared cell lysate were captured in a HisTrap HP 5 ml column (GE Healthcare) in His buffers (20 mM MOPS ...
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bioRxiv - Microbiology 2019Quote: Purified capsids (100 µg) were loaded onto a 5-ml HiTrap™ Heparin-Sepharose HP Column (GE Healthcare), which then washed with Ca2+ and Mg2+-free PBS to remove unbound molecules and eluted with gradually linear increasing concentrations of NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were grown at 37°C and 5% CO2 in high-glucose (4.5 g/L) DMEM (GE Healthcare) supplemented with 10% fetal calf serum (Biochrom ...
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bioRxiv - Biochemistry 2020Quote: ... and the crude lysates were applied to 5 ml HiTrap Capto Q column (GE Healthcare Life Sciences, USA) equilibrated with 5 volumes of buffer A (20 mM Tris (pH 8.0) ...
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bioRxiv - Molecular Biology 2021Quote: ... the supernatant containing complexes of Panx and LC8 was loaded onto 5 ml HisTrap Fastflow column (GE Healthcare). After extensive washing with Binding buffer ...
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bioRxiv - Pathology 2021Quote: ... diluted five-fold with extraction buffer and applied to a 5 ml HisTrap column (GE Healthcare, Chicago, IL) equilibrated with extraction buffer ...
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bioRxiv - Biophysics 2020Quote: ... The EcA2 used for these experiments were previously desalted in a 5-mL HiTrap Desalting (GE Healthcare, USA) and eluted in 10 mM sodium phosphate at pH 7.0 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The lysate obtained was loaded onto the pre-equilibrated nickel affinity column (HisTrap FF, 5 mL, GE Healthcare) with loading buffer (50 mM KPi ...
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bioRxiv - Molecular Biology 2019Quote: ... The cleared lysate was filtered (0.8 µM) and applied to 5 or 10 ml HisTrap FF (GE Healthcare) equilibrated in washing buffer (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was then purified by anion exchange chromatography on a MonoQ 5/50 GL column (GE Healthcare) using buffers AEX-A and AEX-B with a gradient of 7.5-50% B over 20 CV at a flow rate of 1 ml/min ...
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bioRxiv - Biophysics 2020Quote: ... Fractions containing Pol I were then loaded onto a 5-mM HiTrap Heparin HP column (GE Life Sciences) equilibrated in Heparin BufferA(50mMTris-HCl ...
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bioRxiv - Genetics 2019Quote: The clarified lysate containing mZCWPW1 was then loaded onto a 5 mL His-Trap HP column (GE Healthcare) using NGCTM chromatography system (BioRad) ...