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Citations for GE Life Sciences :
1151 - 1200 of 6604 citations for B TFIID TATA Box Binding Protein Associated Factor 1 BTAF1 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... the membranes were then incubated for 1 h with a secondary antibody (goat anti-rabbit horseradish peroxidase-linked IgG [NA934V GE Healthcare] or sheep anti-mouse horseradish peroxidase-linked IgG [NA931V GE Healthcare] ...
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bioRxiv - Physiology 2022Quote: ... membranes were washed three times with 1X TBST and incubated with secondary antibodies in 5% milk in TBST for one hour at room temperature with the following concentration of secondary antibodies: donkey anti-rabbit IgG (1:5000, NA934, GE Healthcare) and goat anti-mouse IgG (1:5000 ...
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bioRxiv - Molecular Biology 2020Quote: ... The membranes were then incubated with HRP-conjugated anti-mouse or anti-rabbit secondary antibodies (1:2500, GE Healthcare Life Sciences) diluted in 2% non-fat milk prepared in PBS for 1 hour at RT followed by 3 washes with TBS containing 0.1% Tween-20 for 5 minutes each at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... After incubating with horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG as a secondary antibody at 1:2000 dilution (GE Healthcare.), protein bands were visualized by chemiluminescence and the image was captured by ImageQuant LAS 4000 mini (GE Healthcare).
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bioRxiv - Physiology 2020Quote: ... Blots were then washed in TBST and incubated for 1 h at 22-24°C with secondary HRP-conjugated antibodies (GE Healthcare) diluted 5000-fold in 5% skim milk in TBST ...
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bioRxiv - Molecular Biology 2021Quote: ... Binding was detected by incubating the plates sequentially with either mouse anti-Histidine Tag antibody (MCA1396, Abd Serotec) followed by horseradish peroxidase (HRP)-linked anti-mouse IgG (1/2000, NXA931, GE Healthcare) or Streptavidin-HRP (554066 ...
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bioRxiv - Cancer Biology 2021Quote: ... or HRP-conjugated anti-mouse (CST, 7076; 1:1000) IgG secondary antibodies and visualized using ECL Prime Western Blotting Detection Reagent (GE Healthcare) and ImageQuant LAS 4000 Mini (FUJIFILM) ...
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bioRxiv - Microbiology 2022Quote: ... and bands visualized with (HRP)- coupled secondary antibody (1:5,000) (Cell Signalling, rabbit 7074S, mouse 7076S) on an ImageQuant LAS 4000 Mini (GE Life Sciences) with Luminata Forte Western HRP Substrate (Sigma WBLUF0500 ...
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bioRxiv - Immunology 2022Quote: ... Membranes were then incubated with the corresponding HRP-tagged secondary antibodies in BSA (5% w/v) PBS-T for 1 hour before visualisation using Amersham ECL prime detection reagent (GE healthcare) and a G:Box Chemi XX6 system (Syngene) ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies for Western blotting were HRP-linked sheep anti-mouse IgG and donkey anti-rabbit (both GE Healthcare; 1:5000) and HRP-linked mouse anti-rabbit ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were washed 3x for 10 min with TBST and subsequently incubated for 1 hr at room temperature with appropriate horseradish peroxidase-conjugated secondary antibodies (GE Healthcare) diluted (1:10,000 ...
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bioRxiv - Cell Biology 2023Quote: ... The following secondary antibodies were used in immunoblotting: HRP-conjugated donkey anti-rabbit IgG (1:2000) (GE Healthcare, catalog number AB_772206), HRP-conjugated goat anti-mouse IgG (1:5000 ...
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bioRxiv - Neuroscience 2023Quote: ... Blots were washed three times in PBST for 15 min and were then incubated with HRP-conjugated secondary antibodies (1:2,500 dilution; GE Healthcare, NA9340V) for 45 min at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The following secondary antibodies were used for immunoblotting: HRP-conjugated donkey anti-rabbit IgG (1:2000) (GE Healthcare, catalog number NA934) and HRP-conjugated donkey anti-mouse IgG (1:5000 ...
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bioRxiv - Plant Biology 2020Quote: ... the cells were lysed by sonication and the fusion protein was purified using HisTrap HP histidine-tagged protein purification column of AKTA pure FPLC system (GE Healthcare, Pittsburgh, PA). The purified protein was further dialyzed overnight and further purified with HiLoad 16/600 Superdex 200 pg column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... Cleavage of the GST tag to induce cleaved fusion proteins was performed using PreScission protease (10 U/mg of fusion protein, GE Healthcare, Uppsala, Sweden) or thrombin protease (10 U/mg of fusion protein ...
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bioRxiv - Biochemistry 2020Quote: ... This cell supernatant was then loaded into protein A affinity column (HiTrap protein A high performance, GE29-0485-76; GE Healthcare Life Sciences,) using liquid chromatography system (GE AKTA Prime Plus) ...
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bioRxiv - Microbiology 2022Quote: ... Protein-protein interaction and protein complex sizes were estimated by comparing the stained protein bands with the native protein molecular weight markers range between 60 to 690 kDa (Amersham, GE Healthcare Life Sciences). Protein bands excised from the stained native protein gels were identified by MS analysis.
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bioRxiv - Molecular Biology 2023Quote: ... The MBP-Q44-HttEx1 protein was purified by fast protein liquid chromatography (FPLC) using a 5 ml HisTrap HP nickel column (GE Healthcare, Uppsala, Sweden) with 0.5 M imidazole gradient (SKU I5513-100G ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant proteins were further purified by gel filtration on an ÄKTA Pure fast protein liquid chromatography (FPLC) system (GE Healthcare Life Sciences). The principal peaks were collected ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were separated on SDS-PAGE and fluorescence was detected from protein gels using the Typhoon FLA 9000 scanner (GE Healthcare Life Sciences) using Cy3 settings (532nm excitation and 610PB filter).
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bioRxiv - Immunology 2024Quote: ... IgG were purified from cell culture supernatant 6-7 days after transfection via Protein A affinity chromatography using 2 mL or 5 mL column HiTrap MabSelect SuRe Protein A column (GE Healthcare, Life Sciences) dependent on culture volume ...
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bioRxiv - Biochemistry 2019Quote: The interaction of the recombinant heterodimeric (‘monomer’) and homodimeric (‘dimer’) human G6b-B extracellular domain with different ligands was quantified using a BIAcoreTM 8K instrument (GE Healthcare, Little Chalfont, UK). Recombinant G6b-B proteins were immobilized on CM5 sensor chips (GE Healthcare ...
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bioRxiv - Plant Biology 2023Quote: ... Active fractions were combined and exchanged into buffer B (50 mM Tris-HCl, pH 7.5) before being applied to a Mono Q™ HR 5/5 column (GE Healthcare, Piscataway, NJ, USA) equilibrated with the same buffer ...
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bioRxiv - Plant Biology 2021Quote: ... Monoclonal anti-actin (plant) antibody detected by the secondary antibody (GE Healthcare; NA931-1ML) was used as a loading control (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: ... The secondary antibodies were HRP-linked antibodies against rat or mouse IgG (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... antibodies and the secondary anti-rabbit horseradish peroxidase-linked IgG antibody (NA944V, GE Healthcare for western blotting and 7074S Cell signaling Technology for IHC ...
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bioRxiv - Neuroscience 2022Quote: ... and 500 μg of total protein from the supernatants was precleared with Protein G Sepharose 4 Fast Flow (GE Healthcare, catalog #GE17-0618-01) for 2 h at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... Single labeled protein was isolated via ion-exchange chromatography (Mono S 5/50 GL, GE Healthcare - protein bound in buffer A (+5 mM βME) and eluted with 0-40% buffer B (buffer A + 1 M NaCl ...
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bioRxiv - Microbiology 2023Quote: ... Importin-α and Ran proteins lacking GST were prepared by cleavage with PreScission protease (20 U/mg of fusion protein; GE Healthcare, Uppsala, Sweden) at 4 °C for 12 h ...
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bioRxiv - Biophysics 2024Quote: ... Single labeled protein was isolated via ion-exchange chromatography (Mono Q 5/50 GL, GE Healthcare - protein bound in buffer A (+5 mM BME) and eluted with 10-40% buffer B (buffer A + 1 M NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... HRP linked antibodies (GE Healthcare), respectively.
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bioRxiv - Biochemistry 2023Quote: ... Anti-GST antibody (GE Healthcare) was used for the detection of GST-tagged bromodomains.
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bioRxiv - Microbiology 2020Quote: ... Proteins were transferred onto polyvinylidene difluoride (PVDF) membrane (GE healthcare). The membrane was blocked with 5% dry milk in PBS containing 0.1% v/v Tween 20 (PBST ...
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bioRxiv - Biophysics 2021Quote: ... on an AKTA Pure 25L protein purification system (GE Healthcare). The column was washed with 10 column volume (CV ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein was purified on an equilibrated HisTrap (GE Healthcare) and step-eluted in wash buffer (100 mM HEPES ...
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Receptor tyrosine kinases regulate signal transduction through a liquid–liquid phase separated statebioRxiv - Cell Biology 2019Quote: ... Proteins were immobilized on Glutathione Sepharose (GE Healthcare Life Science) or StrepTactin™ Sepharose (GE Healthcare Life Science ...
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bioRxiv - Cell Biology 2019Quote: ... Protein A sepharose CL-4B was purchased from GE Healthcare. Antibodies directed toward the following antigens were also purchased ...
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bioRxiv - Cell Biology 2020Quote: ... and proteins were transferred to nitrocellulose membranes (GE Healthcare, 10600007). Antibodies used for western blots are listed in Supplementary Table 1 ...
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bioRxiv - Immunology 2021Quote: ... and protein bands were visualized with ECL Prime (GE Healthcare) or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... supernatants were purified using Protein G Sepharose beads (GE Healthcare), dialyzed against PBS ...
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bioRxiv - Genetics 2021Quote: ... proteins were detected with Amersham ECL Prime (GE Life Sciences). The following antibodies were used ...
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bioRxiv - Genetics 2021Quote: ... Proteins were transferred to Hybond-P PVDF membranes (GE Healthcare) in 1 × NuPAGE Transfer Buffer (Invitrogen by ThermoFisher ...
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bioRxiv - Biophysics 2021Quote: ... Protein was purified via FPLC (AKTA prime system, GE Healthcare) using HisTrap HP columns and eluted in 25 mM Tris ...
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bioRxiv - Biophysics 2020Quote: ... The digested protein was subjected to Superdex 200 (GE Healthcare) size exclusion chromatography in PBS to yield to pure anchor unit.
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bioRxiv - Microbiology 2020Quote: ... Proteins were transferred on a PVDF membrane (GE Healthcare, 10600021) via wet electroblotting at 100 V in transfer buffer (20% methanol ...
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bioRxiv - Neuroscience 2021Quote: ... and proteins were electrophoretically transfected onto nitrocellulose membranes (GE Healthcare). Membranes were blocked in blocking buffer (Li-COR Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were detected with the AI-600 image (GE Healthcare) using SuperSignal West Dura Extended Duration Substrate (Thermo) ...
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bioRxiv - Neuroscience 2021Quote: ... Separated proteins were transferred onto nitrocellulose membranes (GE Healthcare Amersham) which were subsequently blocked in 5% milk PBS for 1 h at room temperature before being probed with relevant antibodies O/N at 4°C in 5% milk PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... on Protein G Sepharose 4 Fast Flow beads (GE healthcare). The samples were incubated at 4°C overnight and then washed with progressively more stringent conditions ...